Princeton Digital (USA) 4411-0062 manual

4411-0062 Version 3.A February 11, 2004 *4411-0062*[...]

© Copyrig ht 2001-2004 Princeton Instrum ents, a div ision of Roper Scientific, Inc. 3660 Quakerbridge Rd Trenton, NJ 08619 TEL: 800-874- 9789 / 609-587- 9797 FAX: 609-587- 1970 All rights reserv ed. No part of this publication m ay be reproduced by any m eans without the w ritten permission of Princeton I nstrum ents, a div ision of Roper Scienti[...]

...SEE the Future 3660 Quakerbridge Road | Trenton, NJ 08619-1208 | tel 609.587.9797 | fax 609.587.1970 | www.princetoninstruments.com Camera Detection Wizard Introduction Version 2.5.19.6 of the WinX software (WinView , WinSpec, and WinXTest) introduced enhancements to the former Hardware Setup Wizard. Now called th e Camera Detection Wizard, this[...]

...SEE the Future Camera Detection Wizard July 27, 2005 2 of 2 Princeton Instruments E:/Manuals/Tech Notes Camera Detectio n Wiz ard supplement.doc Camera Detection Wizard Flowchart . Optional Configuration Disk? Yes Make sure camera system is connected to host computer and system is turned ON. Computer Interface Selection (RS PCI) Controller Sele[...]

iii Table of Contents Chapter 1 Introduction .........................................................................................9 Descrip tion.................................................................................................................... ...... 9 About this Manual ..........................................................[...]

iv I - Penta MA X System Manual Version 3.A Chapter 4 Temperature Control ........................................................................33 Introduc tion................................................................................................................... .... 33 Air Cooling ....................................................[...]

Table of Contents v Im age Readout with Binning...................................................................................... 66 Binning in Software ................................................................................................... 67 Frame Transfer Readout ...................................................................[...]

vi I-Pe n t a MAX System Manual Version 3.A Version 4 ...................................................................................................................... ..... 87 Version 5 ...................................................................................................................... ..... 87 Appendix D Two-Shot Kinetics M[...]

Table of Contents vii Figure 14. Chart of Full Speed and Safe Mode Operation .............................................. 53 Figure 15. Freerun Timing chart ( pa rt of the chart in Figure 14) ................................... 54 Figure 16. Freerun Ti ming diag ram................................................................................. [...]

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9 Chapter 1 Introduction Figure 1. I-PentaMAX Sys tem Descriptio n The Princeton Instrum ents I - Penta MAX System consists of an intensified cam era, an external temperature controller/power supply , a com puter, a high- voltag e power supply and the necessary sy stem cables. I t is desig ned for general m acro- im aging and microscopy im aging ap[...]

10 I- Penta MA X System Manual Version 3.A Switching between the two ch annels is com pletely under software control for total experiment autom ation. Data is transferred di rectly to the host com puter m emo ry v ia a high- speed serial link. Standard com pos ite video, either RS- 170 (EI A) or CCI R, whichever was ordered, is also prov ided. The [...]

Chapter 1 Introduction 11 About this Manua l Manual Organization This manual prov ides the user with all th e inform ation needed to install an I - Penta MAX system and place it in operation. Topics covered include a detailed description of the camera, the tem perature/power supply unit, installation, cleaning, specifications and more. Chapter 1, I[...]

12 I- Penta MA X System Manual Version 3.A Safety Re lated Symbols Used Manua l Caution! The use of this sym bol on equi pment indicates that one or more nearby item s should not be operated without first consulting the manual. The sam e sym bol appears in the m anual adjacent to the text that discusses the hardware item(s) in question. Caution! Ri[...]

Chapter 1 Introduction 13 Camera Back Panel The camera’s connectors and coolant ports ar e located on the back panel as shown in Figure 2. A brief description of each feature follow s. Fan: There is an internal fan located behind th e back panel next to the ventilation g rill. Its purpose is: • to remo ve heat from the Peltier device that cools[...]

14 I- Penta MA X System Manual Version 3.A HIGH SPEED SERI AL Connector: The cable that goes from the C amera to the computer connects to this 9- pin connector . Again, it is essential that the cable connector locking screws be tightened securely to ensure reliable operation. I f the application requires use of the optional fiber-optic data link to[...]

Chapter 1 Introduction 15 LOGIC OU T BN C Connector: This TTL output (formerly labeled NOTSCAN ) is provided to allow m onitoring of the cam era’s status. The camera state reported at this connector is selected by the applic ation software. A brief description of the available sig nals follows. NOTREADY: After a Start Acquisition comm and, th is [...]

16 I- Penta MA X System Manual Version 3.A CCD and Intensifier Enc losure The camera is shipped back filled to 1 psi (gaug e), which is g ood for the lifetime of the camera. I f the cam era intensifier/CCD enclosure should accidentally be opened to the atmosphere, im m ediately shut down the system . Contact Prin ceton I nstrum ents Customer Suppor[...]

Chapter 1 Introduction 17 may still differ slig htly once tem perature lock has been established, typically by 0.1 ° C. This difference stems from the panel- m eter limitations. ACTUAL vs. SET POI NT Switch : This switch allows the user to choose either the Set Point tem perature or the Actual tem perature for display . TEMP SET Knob: This knob di[...]

18 I- Penta MA X System Manual Version 3.A Temperature/Power Supply Back Panel Fan: The internal fan that cools the Temper ature/Power Supply elect ronics is located directly behind the back -p anel grill. This fan runs continuously w henever the power is on. For proper operation, it is essen tial that free circulation be maintained between the rea[...]

Chapter 1 Introduction 19 Note that there is no provision for setting the operating line voltag e. None is required because the I- Penta MA X Temp erature/Power Supply unit auto- senses the applied vo ltage and automatically configures itself according ly. However, the line fuse v alue does depend on the line voltag e as indicated to the right of t[...]

20 I- Penta MA X System Manual Version 3.A ESD Precautio ns The CCD and other sy stem electronics are ex tremely sensitive to electrostatic discharg e (ESD). To avoid perm anently dam aging the system , please observ e the following precautions: • When using hig h- voltag e equipm ent (such as an arc lamp) with y our I - Penta MAX system , be sur[...]

Chapter 1 Introduction 21 Computer Re quireme nts Host Computer Type Note: Computers and operating sy stem s all undergo frequent rev ision. The following information is only intended to giv e an approximate indication of com puter requirements. Please contact the factory to determ ine your specific needs. PC Type: PCI -bus based Pentium (or better[...]

22 I- Penta MA X System Manual Version 3.A Cleaning a nd Maintena nce Turn off all power to the equipment and secu re all cov ers before cleaning the units. Otherwise, damag e to the equipm ent or injury to you could occur. Temperature/Power Supply Periodic cleaning of the Tem perature/Power Supply unit filter is required to ensure adequate airflow[...]

23 Chapter 2 Installation Overview The list and diagram s below briefly describe the sequence of actions required to hookup your system and prepare to gather da ta. Refer to the indicated references for more detailed inform ation. Action Reference 1. If the sy stem com ponents hav e not already been unpack ed, unpack them and inspect their carton(s[...]

24 I- Penta MA X System Manual Version 3.A Action Reference 12. Connect the TAXI ™ cable to the camera and the interface card in the host computer. Then tig hten down the locking hardware. Chapter 3, page 31 13. If the sy stem is cooled by cool ant circulation, mak e the tubing connections between the coolant circulator and the cam era. Chapter 4[...]

25 Chapter 3 Hardware Setup Introduct ion This chapter is provided to help y ou get started with y our I- Penta MAX System . In addition to descriptions of such basics as unpacking and g rounding safety , the chapter includes discussions of the requirements that hav e to be met before the cam era can be powered. Included are env i ronmental, power,[...]

26 I- Penta MAX System Manual Version 3.A • Camera to High Volt age supply cable: Normally supplied with the high -v oltage power supply or pulser. • System Dependent Interf ace Components: Note: A n I-P e n ta MAX system requires a computer, which could be any one of several different ty pes, each requiring a di fferent application software pa[...]

Chapter 3 Hardware Setup 27 Figure 6. Power Input As sembly (Fus e Access) 4. Remov e the fuse and check to be sure th at its current rating is correct for the intended operating v oltage. Replace the fuse if necessary . 5. After verify ing that the fuse is correct, or after installing the new fuse in the carrier, should that be necessary, insert t[...]

28 I- Penta MAX System Manual Version 3.A Mounting the Ca mera General The I- Penta MAX cam era can be mounted either horiz ontally or v ertically (nose up or nose down). The camera can rest on any secure surface. Also, there is a standard ¼ ″ × 20 UNC thread 5/8 ″ deep hole on the bottom of the cam era behind the lens mount for mounting vers[...]

Chapter 3 Hardware Setup 29 Lens release lever Front part of adapter for adjusting focus Set screws (4) to lock front part of adapter in place Figure 7. F-mount L ens Adapter To install an F-mount lens on the camera: 1. Locate the large indicator dot on the side of the lens. 2. Note the corresponding dot on the front side of the camera lens m ount.[...]

30 I- Penta MAX System Manual Version 3.A Installing the Ap plication Softw are Driver Installation Installation is perform ed via the WinView/ 32 installation process, which should be done before the interface card is installed in the host computer. On the Select Components dialog box (see below), click on the button appropriate for the interface [...]

Chapter 3 Hardware Setup 31 To Ins tall a PC I Card : If using WinV iew/32 software, either High Sp eed PCI or PCI( Timer) can be the selected Interface ty pe. T his selection is accessed on the Hardware Set up | Interf ace tab page. High Speed PCI allows data transfer to be interrupt-driv en and giv es the highest performance in m ost situations. [...]

32 I- Penta MAX System Manual Version 3.A Connecting t he Camera to HV Supply Cable To Connect the Camera to HV Pow er Supply Cable: 1. Verify that the HV supply unit power is OFF and that the Temperature/Power Supply unit power is OFF. 2. At the Intensifier H.V.P.S. connector on the rear of the HV power supply, align the cable connector so the plu[...]

33 Chapter 4 Temperature Control Introduct ion As described in Chapter 1, Introduction , temperature control in the I- Penta MAX system is quite straightforward. Typically , if the T em perature Control On/Off switch has been set to ON, it is only necessary to set the temperature display mode switch to SET POINT and then adjust the TEMP SET knob un[...]

34 I- Penta MAX System Manual Version 3.A Water Cooling Supplemental water cooling can be implem ented v ia the camera’s back panel two cooling ports, either of which can be used for inflow or outflow. Connect to the ports using ¼ ″ inner diam eter T yg on tubing secured w ith a suitable clam p (barb-sleev e clamps (2518-0301) are prov ided). [...]

35 Chapter 5 First Light Introduct ion This chapter provides a step- by- step procedure for placing the I- Penta MAX system in operation for the first time. At this point a lens should be m ounted on the camera (or, if necessary, the cam era mounted on a microscope) and y ou should be ready to operate the system and proceed to viewing your first im[...]

36 I- Penta MAX System Manual Version 3.A Over expos ure Pr otec tion Im age intensifiers can be destroyed if continuously exposed to lig ht levels hig her than twice the A/D saturation lev el. T o prevent dam age to the camera, check that the following conditions are m et before making any system connections. ➧ Any HV pulsers present are turned [...]

Chapter 5 First Light 37 Shutter mode defines an intensifier biasing mode. It doesn’t m ean or imply that the system contains a mechanical shutter. In Shutter m ode operation, with no signal applied to the SHUTTER IN connector of the II C- 200 ( II C-300, I IC -100, or MCP- 100), the intensifier is biased on continuously and the cam era “sees l[...]

38 I- Penta MAX System Manual Version 3.A The high voltag e cable should be handled w ith care. Dropping the cable or bang ing the connectors may damage the pins, resulting in a poor or intermittent connection. • Connect one end of the 9-pin serial cable to the HIGH SPEED SERIAL connector. Connect the other end to the computer interface. Note: If[...]

Chapter 5 First Light 39 is turned on, the POWER light at the front panel of the Temperature/Power Supply unit should light. 6. Start the application software (WinView, for exam ple). 7. At the front panel of the Temperature/Po wer Supply , set the temp erature display mode switch to SET POINT and the Temperature Control ON/OFF switch to ON . As so[...]

40 I- Penta MAX System Manual Version 3.A 11. At the computer, config ure the applicati ons software for the I- PentaMAX cam era. The following setting s are for WinView: Hardw are Set up|Controll er/CCD: Controller Type: PentaMAX Controller Versio n: version of your controller CCD Type: appropriate frame transfer array (EEV 512 × 512FT, for examp[...]

Chapter 5 First Light 41 • In crease gain to about 1/2 m axi mum on the dial or the PG-200. • Adjust the lens aperture until a suitable value is found. Check the brightest regions of the im age to determine if the A/D conv erter is at full-scale. Adjust the aperture just slightly sm aller (highe r f-stop) than the setting where maxim um brightn[...]

42 I- Penta MAX System Manual Version 3.A Imaging Field of Vie w When used for two-dim ensional imaging applications, the I-P enta MAX cam era closely imitates a standard 35 m m cam era. Since the CCD is not the same siz e as the film plane of a 35 mm camera, the field of view at a giv en distance is somewhat different. D O B S Object Lens CCD Figu[...]

43 Chapter 6 Microscopy Applications Introduct ion This chapter discusses the setup and optimiz ation of your dig ital im aging system as applied to microscopy . Since scientific grade cooled im aging system s are usually employ ed for low light lev el microscopy , the m ajor goal is to maxim ize th e light throughput to the cam era. In order to do[...]

44 I- Penta MAX System Manual Version 3.A The adapter can be screwed into the camera a nd then the assem bly can be secured to the microscope using the standard set screws on the m icroscope. The camera can be m ounted on the trinocular output port, the side port or the bottom port of the inv erted m icroscope. When mounting the camera perpendicula[...]

Chapter 6 Microscopy Applications 45 This whole assembly can now be placed on the microscope, using the appropriate setscrews on the m icroscope to secure the bottom clam p to the output port of the microscope. The F-m ount is appropriate for any trinocular output port or an y side port. When mounting the camera perpendicular to the microscope on t[...]

46 I- Penta MAX System Manual Version 3.A 1X HRP 100-NIK "L" bottom clamp Figure 11. Bottom C lamp secured to Relay Lens Operatio n Xenon or Mercury Arc Lamp Precautions Before You Start , if your sy stem includes a micros cope Xenon or Hg arc lam p, it is CRITICAL to turn off all electronics adj acent to the arc lamp, especially your dig[...]

Chapter 6 Microscopy Applications 47 Adjusting the Parf ocality of th e Camera On a C-m ount system, the cam era should be very close to parfocal, although som e C- mounts w ill be adjustable using set screws on the m i croscope to secure the adapter slightly higher or lower in position. To adjust the parfocality on an F-m ount system, beg in colle[...]

48 I- Penta MAX System Manual Version 3.A Micros copes a nd Infra red Light Microscope optics have v ery hig h transm issi on efficiencies in the infrared region of the spectrum. Since the lig ht sources are very good em itters in the infrared, some microscopes are equipped with I R blockers or heat filters to prevent heating of optical elements or[...]

49 Chapter 7 Intensifier Overview of In tensifier Operation The modern im age intensifier results from a c oncerted effort undertak en to develop nig ht vision capabilities for m ilitary applications a nd to this day the primary application remains nig ht vision – the ability to see in low lig ht level conditions. Basically , an intensifier is a [...]

50 I- Penta MAX System Manual Version 3.A Figure 13 illustrates m icrochannel operation. T he channels function in much the sam e manner as the dy node stage in a photomultiplier tube throug h the use of a secondary emitter. Each tim e the electron strikes the wall of the tube, it strikes a secondary em itter, which causes a shower of electrons. Th[...]

51 Chapter 8 Timing Modes The Princeton Instrum ents I- Penta MAX sy stem has been designed to allow the g reatest possible flexibility when sy nchronizing da ta collection with an experim ent. The chart below lists the tim ing m ode combina tions. Use this chart in com bination with the detailed descriptions in this chapter to determine the optim [...]

52 I- Penta MAX System Manual Version 3.A Full S peed (syn c) or Safe Mo de (async) Selection of Full Speed (formerly known as Synchronous m ode) or Safe Mode (formerly known as Asy nchronous m ode) determines the ov erall control of experiment timing . In Full Speed mode, the I -Penta MAX runs according to the tim in g of the experiment, with no i[...]

Chapter 8 Timing Modes 53 Background or flatfield on? Start (Saf e Mode) Computer programs camera with exposure and binning parameters ST ART ACQ issued from computer to camera Cleans performed 1 frame collected as per timing mode Background and/or flatfield correction performed Frame displayed Frames complete? Stop Ye s Ye s No No STOP ACQ issued [...]

54 I- Penta MAX System Manual Version 3.A time betw een the External Sync pulse and the ev ent is less than a few m illiseconds, the time it tak es the shutter to open. The shutter timing is shown in the tim ing di agram s that follow. Except for Freerun, where the modes of shutter operation are id entical, both Normal and Preopen lines are shown i[...]

Chapter 8 Timing Modes 55 External Sync Timing In this m ode all exposures are synchronized to an external source. As shown in the flow chart, Figure 17, this m ode can be used in combination with Norm al or Preopen Shutter operation and with Continuous Cleans. In Norm al Shutter mode, the controller waits for an External Sync pulse, then opens the[...]

56 I- Penta MAX System Manual Version 3.A cleaning, g enerating pulses with a frequency equa l to the vertical shift tim e of the device being used. This characteristic m akes NOTSCAN unsuitable for inhibiting a pulser w hile the array is being read out. Use the SHUTT ER signal i nstea d (NOTSCAN a nd SHUTTER can also be provided at the I- Penta MA[...]

Chapter 8 Timing Modes 57 NOTSCAN (Continuous Cleans ON) Shutter (Normal) Open Close Open Close Open Close Read Read Read Shutter (Preopen) Open Close Open Close Open Close External Sync (negative polarity shown) Clean Clean Clean Figure 19. Exter nal Sync (C ontinuous Cleans O N) T iming diagr am Softwar e Trigge r The third timing mode available [...]

58 I- Penta MAX System Manual Version 3.A less than one divided by the scan time. The m i nimu m exposure tim e is equal to the amount of tim e needed to read out the storage ha lf of the array, unless an external shutter is used. Figure 20 shows an exam ple where t exp + t w1 + t c < t R . t w1 is the time the controller waits for the first pul[...]

Chapter 8 Timing Modes 59 Data not stored External Sync (negative polarity shown) NOTSCAN Read Read Read Read Data stored Data stored Data stored SHUTTER t exp t w1 t R t c Figure 22. Frame T ransfer where Sync. Puls e arrives after Readout Edge vs. Level External Sync In the prev ious figures describing External Sy nc timing modes in the I- Penta [...]

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61 Chapter 9 Exposure and Readout Before each im age from the CCD array appears on the com puter screen, it must first be read, digitiz ed, and transferred to the computer. A block diagram of the path of the image signal is shown in Fig ure 23. Fast Analog Signal Processor Camera Computer Intensifier CCD Preamp Fast A/D Slow A/D HS serial interface[...]

62 I- Penta MAX System Manual Version 3.A Exposur e Introduction Charge coupled dev ices can be roughly t houg ht of as a two-dim ensional grid of individual photodiodes (called pixels), each conne cted to its own charg e storage “well.” Each pixel senses the intensity of lig ht falling on its collection area, and stores a proportional amount o[...]

Chapter 9 Exposure and Readout 63 the II C-200 (I I C- 300, II C-100, or MCP- 100), the in tensifier can be turned ON or OFF in much the sam e way as it is in Gated operati on, but at slower speeds, allowing exposures from 50 µ s to 23 hours to be set from software. "Smearing " of signal features can occur if the CCD is exposed to lig ht[...]

64 I- Penta MAX System Manual Version 3.A Note: Do not be concerned about either the DC level of this back ground or its shape unless it is very high, i.e., > 200 counts. Wh at y ou see is not noise. It is a fully subtractable readout pattern. Each CCD has its own dark charge pattern, unique to that particular device. Sim ply turn off the intens[...]

Chapter 9 Exposure and Readout 65 1 2 3 4 A1 A2 A3 B1 B2 B3 C1 C2 C3 D1 D2 D3 A4 A5 A6 B4 B5 B6 C4 C5 C6 D4 D5 D6 A1 A2 A3 B1 B2 B3 C1 C2 C3 D1 D2 D3 A4 A5 A6 B4 B5 B6 C4 C5 C6 D4 D5 D6 A1 A2 A3 B1 B2 B3 C1 C2 C3 D1 D2 D3 A4 A5 A6 B4 B5 B6 C4 C5 D4 D5 C6 A1 A2 A3 B1 B2 B3 C1 C2 C3 D1 D2 D3 A4 A5 A6 B4 B5 B6 C4 C5 C6 D4 D5 D6 Figure 24. Full Fr ame [...]

66 I- Penta MAX System Manual Version 3.A The readout times for an EEV 512 × 512 array are prov ided in Table 4 below. CCD Array 1 MHz Readout Time 5 MHz Readout Time EEV 512 × 512 0.28 sec. 0.07 sec. Table 4. Approxim ate Readout Time for the CC D Array A subsection of the CCD can be read out at full resolution, sometim es dramatically increasin[...]

Chapter 9 Exposure and Readout 67 1 2 3 4 A1 A2 A3 B1 B2 B3 C1 C2 C3 D1 D2 D3 A4 A5 A6 B4 B5 B6 C4 C5 C6 D4 D5 D6 A1 A2 A3 B1 B2 B3 C1 C2 C3 D1 D2 D3 A4 A5+A6 B4 B5+B6 C4 C5+C6 D4 D5+D6 A1 A2 A3 B1 B2 B3 C1 C2 C3 D1 D2 D3 A4 B4 C4 D4 A1 A2 A3 B1 B2 B3 C1 C2 C3 D1 D2 D3 A4 A5+A6 B4 B5+B6 C4 D4 C5+C6 +D5+D6 A5+A6 +B5+B6 Figure 25. 2 × 2 Binni ng for[...]

68 I- Penta MAX System Manual Version 3.A Software averag ing can improv e the S/N ratio by as m uch as the square root of the number of scans. Unfortunately , with a high number of scans, i.e., abov e 100, camera 1/f noise may reduce the actual S/N ratio to slightly below this theoretical v alue. Also, if the light source used is photon- flicker r[...]

Chapter 9 Exposure and Readout 69 digitiz ed. The percentage of sm earing is given by the equation below, sim ply the time needed to shift all rows from the im agin g area div ided by the exposure tim e. (6) Digitiza tion During readout, an analog si gnal representing the charg e of each pixel (or binned group of pixels) is digitiz ed. The number o[...]

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71 Chapter 10 Troubleshooting Introduct ion The following issues have corresponding tr oubleshooting sections in this chapter. Alarm Sounds Sporadically Page 72 Alarm Sounds Continuously Page 72 Baseline Signal Suddenly Changes Page 72 Camera Stops Work ing Page 72 Controller Is Not Responding Page 73 Error Indicator Lig hts on Temperature/Power Su[...]

72 I- Penta MAX System Manual Version 3.A Alarm Sounds Spor adically It is norm al for the alarm to sound briefly when the hig h- voltag e supply is first turned on. However, if the alarm sounds sporadically, c ontact the factory at once. This may indicate intensifier damag e or another situa tion that requires immediate attention. Alarm Sounds Con[...]

Chapter 10 Troubleshooting 73 Controller Is Not Responding If this m essage pops up when you click on OK after selecting the Interface Type during Hardware Setup (under the WinView/32 Setup menu), the system has not been able to comm unicate with the camera. Check to see if the Tem perature/Power Supply unit has been turned ON and if the interface [...]

74 I- Penta MAX System Manual Version 3.A at a temperature colder than the specified lim it. Adjust the operating environm ent temperature. • The Temperature/Power Supply has ov erhe ated; the most likely cause would be obstructed ventilation. There m ust be free access between the air in the room and the air intake on the bottom of the Tem perat[...]

Chapter 10 Troubleshooting 75 available addresses and interrupt lev els and au tomatically assigns them so that there are no PCI address or interrupt conflicts. Howeve r, because the BIOS doesn' t know about the user-assig ned ISA I/O address and interrupt level assig nm ents, it is possible that a PCI card will be assigned an address or inter[...]

76 I- Penta MAX System Manual Version 3.A Note: Changing the order of the PCI cards, that is, plugging them into different slots, could change the address and interrupt assi gnm ents and possibly resolve the conflict. However, this would be a trial and erro r process with no g uarantee of success. Diagnostics Software Many diag nostics program s, b[...]

Chapter 10 Troubleshooting 77 Excessive Readout Noise Excessive readout noise with the intensifier off indicates possible m oisture accum ulation in the CCD. This should be corrected promptly or perm anent damage not cov ered by the Warranty could occur. Norm al cam era noise is a function of the ga in setting and tem perature as well as CCD type, [...]

78 I- Penta MAX System Manual Version 3.A Figure 27. Power Input As sembly: Fuse Acces s Temperature Lock Cannot be Achieved or Maintained Possible causes could include: • High ambient tem perature. • Airflow through the cam era is blocked. • The camera fan is not running . • The Temperature/Pow er Supply filter is dirty . • The connector[...]

Chapter 10 Troubleshooting 79 • You are attem pting to operate at a temp er ature colder than the specified limit. TE-cooled cam eras are equipped with a therma l- protection switch that shuts the cooler circuits down if the internal tem peratur e exceeds a preset limit. Typically , camera operation is restored automatically in about ten minutes.[...]

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81 Appendix A Specifications Intensifier * Types Gen II: 18 m m gatable; resolution 30 line pairs per m m. Hig her resolution tubes are available Gen III: 18 m m gatable; resolution 50 line pairs per m m Spectral Range Gen II: Red- blue enhanced, 180-800 nm; Red enhanced, 360- 920 nm Gen III: 500- 900 nm Method of Coupling 1.5:1 fiber optics Vignet[...]

82 I- Penta MAX System Manual Version 3.A CCD Array EEV CCD-37 Format: 512 × 512 × 2; 7.7 × 7.7 mm overall; 15 × 15 µ m pixels Temperatu re Contro l Setting Mechani sm: Temperature is set by fric tion-lock dial on front of Temperature/Power Supply . Display: Digital display on front of Temperature/Power supply displays either the set temperatu[...]

Appendix A Specifications 83 Outputs VIDEO: 1 V pk- pk from 75 Ω , BNC connector. Either RS- 170 (EI A) or CCIR standard video as specified when system wa s ordered. Requires connection via 75 Ω cable that must be term inated in 75 Ω . LOGIC OU T: TTL output (BNC) for monitoring th e camera status. The camera state being reported is selected [...]

84 I- Penta MAX System Manual Version 3.A Miscella neous Dimensions: See Appendix B. Camera Weight: 4.1 kg . Power Supply Weight : 3.2 kg Power Requirements: 105- 125 or 210- 250 V AC (autos ense selection), 47-63 Hz, 200 watts maxim um; required DC levels prov ided by power supply reg ulators. Power to camera is applied v i a cable between 25- pin[...]

85 Appendix B Outline Drawings of Camera & Temperature/Power Supply Note: Dimensions are in inches (mm ). Figure 28. I-Penta MA X : C-Mount[...]

86 I- Penta MAX System Manual Version 3.A Figure 29. I-Penta MA X : F-Mount 1 1.74 (298.2) 4.90 (124.5) 6.42 (162.9) Figure 30. Temper ature/Pow er Supply[...]

87 Appendix C Penta MAX Versions Introduct ion With time, the P enta MAX has ev olved enhanced capabilities as reflected in the Version number of the instrum ent. To access the full ca pabilities, it is necessary that the Version be correctly selected in software. This can be easily done from the Hardware Setup pages of WinView/32 and WinSpec/32. A[...]

88 I- Penta MAX System Manual Version 3.A Read back of Se rial NVR AM System ID informatio n: This EEPROM must be program med by production test/system test using the “NVRAM Windows Application,” provided by the softwa re department. This EEPROM will hold a variety of information about the cam era system w hich will allow the hardw are version [...]

Appendix C Penta MAX Versions 89 Virtual chi p operation: This feature allows a portion of the array to be redefined as a "virtual" chip for enhanced data acquisition speed. Fram e rates in excess of 100 frames per second can be obtained.[...]

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91 Appendix D Two-Shot Kinetics Mode Princeton Instrum ents now supports a 2-shot kinetics m ode in the PentaMAX ICCD system through a mini- application called PMX ICCD Kinetics App. The basic performance is as follow s: ➧ Hardwired for PentaMAX 512 Fram e Transfer Camera, Version 5. This includes the PentaMAX Gen III IC CD, Version 5. ➧ Progra[...]

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93 Appendix E Virtual Chip Mode Introduct ion Virtual Chip mode (a WinView/32 option) is a special fast-acqui sition technique that allows frame rates in excess of 100 fps to be obtained. For the Virtual Chip selection to be present, it is necessary that: • the system be a PentaMAX, I- PentaMAX, Micr oMAX (1 MHz or 5 MHz) or ST-133, • that the [...]

94 I- Penta MAX System Manual Version 3.A REGION msec/ FRAME FPS* 160 × 160 7.07 141 98 × 98 3.08 324 89 × 89 2.72 367 68 × 68 1.74 574 56 × 56 1.39 719 51 × 51 1.14 877 41 × 41 0.92 1087 38 × 38 0.78 1282 32 × 32 0.68 1470 32 binned × 512 0.52 1923 Table 9. I-PentMAX, 5 M Hz: Vir tual Chip Siz e and Approximate N umber of Fr ames per Sec[...]

Appendix E Virtual Chip Mode 95 Assumptions: • You are fam iliar with the WinView/32 software and have read the hardware manuals. • Masking is for a 41x41 pixel V irtual Chip with its origin at 1,1. System Connection Diagram: FROM PO WER SUPPL Y HOST COMPUTER LOGIC OUT** HV CABLE EXPERIMENT TEMPERA TURE/ PO WER SUPPL Y IIC-200 T O CAMERA SHUTTE[...]

96 I- Penta MAX System Manual Version 3.A 6. Connect the Camera-P ower Supply cable to the TO CAMERA connector on the rear of the Temperature/ Power supply unit and to the FROM POWER SUPPLY connector at the rear of the cam era. Tighten down the locking screws. 7. If it has not been installed already , connect a line cord from the Power I nput assem[...]

Appendix E Virtual Chip Mode 97 Controll er/CCD t ab card • Controller: PentaMAX • Controller Versio n: 5 • CCD Type: appropriate frame transfer array (EEV 512x512FT, for this procedure) • Shutter Ty pe: None • LOGIC OUT Output: Shutter • Readout Mode: Fram e T ransfer Interface tab card • Type: the appropriate interface card. For thi[...]

98 I- Penta MAX System Manual Version 3.A • Observe the calculated readout tim e. If you need a shorter period, chang e the settings (for exam ple, enter a smaller Y- dimension or use binning in the Y-direction) and click on the Download Virtual Chip Definition button again. • Click on Close . 18. Fr om the Ac quisition menu, sel ect Exper imen[...]

Appendix E Virtual Chip Mode 99 22. Place a suitable target in front of the camera and click on Focus to verify that the camera is seeing the target. 23. Make any focusing, gain, or other adjustm e nts necessary to fine-tune the im age. 24. Stop running in Focus mode. 25. Now click on Acquire . Exper imenta l Timing Triggering can be achieved throu[...]

100 I- Penta MAX System Manual Version 3.A Chip Y Dimension: This is the Y range established by the external m ask. Chip X Dimension: This is the X range established by the external m ask. ROI: The X and Y Start, End and Binning (Gr oup) values can be entered. The ROI can be as large as the v irtual chip area established by the external m ask or a [...]

Appendix E Virtual Chip Mode 101 ➧ If fram e acquisition appears to be slow in Focus mode, check the Fram es/I nterrupt value on the Env ironment dialog box and reset the value to 1 if it is greater than 1. ➧ When processing larg e stacks of data, y ou may want to use a third- party scientific imag e processing package. ➧ Use the 15' HV [...]

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103 Warranty & Service Limited War ranty Princeton Instrum ents, a division of Roper Scie ntific, Inc. ("Princeton I nstruments," us," "we," "our") m akes the follow ing lim ited warra nties. These limited warranties extend to the original purchaser ("You", "y ou") only a nd no other purcha[...]

104 I- Penta MAX System Manual Version 3.A Sealed Chamber Integrity Limited 24 Month Warranty Princeton Instrum ents warrants the sealed ch amber integrity of all our products for a period of twenty- four (24) months after shipment. I f , at anytim e within twenty-four (24) months from the date of delivery, the dete ctor should experience a sealed [...]

Warranty & Serv ice 105 Owner's Manual and Troubleshooting You should read the owner’s manual thoroug hl y before operating this product. In the unlikely event that y ou should encounter di fficulty operating this product, the owner’s manual should be consulted before contac ting the Princeton Instruments technical support staff or aut[...]

106 I- Penta MAX System Manual Version 3.A 10. All warranties implied by state law or non-U.S. laws, including the im plied warranties of m erchantability and fitness for a particular purpose, are expressly limited to the duration of the lim ited warrantie s set forth above. With the exception of any warranties im plied by state law or non-U.S. law[...]

107 Index A/D con verters, 69 dual, 69 specification s, 83 AC pow er requirem ents , 26 Actual ex posure tim e, 57 Actual vs Set Point sw itch, 17 Air circulati on requi remen t, 13, 18, 19, 22 cooling , 33 filter, 19, 22 Analog chann els, 69 Asynchronous mod e. See Safe Mode Autosensing line voltage, 26 Backfill pressure, 16 Bac kground DC level, [...]

108 I- Penta MAX System Manual Version 3.A Cool ing supplem ental, 34 Dark charg e definition of, 63 dynamic ra nge, 6 3 kT no ise, 63 temperatu re dependence, 63 typical v alues, 63 Dark curren t, 63 Descriptio n of system, 9 Digitization, 69 DMA bu ffer, 98 Dual A/ D converters , 69 Dynamic range , 63 EBI, 62 EISA- bus, 21, 83 EMF spik e, 35, 46 [...]

Index 109 Indicators ERROR, 1 7 POWER, 17 STATUS, 17, 33, 34, 73 Installation interface PCI, 30 Intensifier alarm , 36, 50 descripti on of , 9 EBI, 62 MCP, 62 overview of operation, 49 Interface board, 26 Interface card driver installation, 30 ISA, 30 PCI, 30 High Spee d P CI, 31 PCI(Timer), 31 troubles hooting, 74 Interrupt conflicts, 74 I-PentaM [...]

110 I- Penta MAX System Manual Version 3.A Pow er Suppl y con nector, 13 Po wer suppl y, high volta ge IIC-100, 12, 37, 96 IIC-200, 12, 37 MCP-100, 12, 37, 96 Preopen Sh utt er m ode, 55 Procedures fus e replacemen t, 26, 77 Readout binning, 66 hardw are, 66 digitization, 69 fram e transf er, 68 rate, 69 subs ection of array, 66 Readout rate contro[...]

Index 111 Video BNC con nector, 14 Virtual Chip mode setu p, 94 softw are option, 93 sy stem connection diagram, 95 WXvch ip.opt f ile, 93 War nings cable han dling , 12, 38, 96 cleaning , 22 damage a t high light levels , 36 damage fr om expo sure t o exce ssive light levels, 25 EMF sp ike da mage from Xeno n or H g arc lam ps, 46 high volt age d [...]

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Princeton Instruments 1 of 2 May 12, 2004 E:Man ualsC ontrol lerS T-133BS T-133B Add endum .doc ST-133 Controller Addendum Introduction Thank you for purchasing a Princeton Instruments cameras system. Princeton Instruments is presently phasing in a new improved vers ion of the ST-133 Controller. Depending on the ship date of your system and the[...]

ST-133B Addendum May 12, 2004 2 of 2 Princeton Instruments CE:M anuals Contro ller ST-133B ST-133B Ad dend um.doc Figure 2. Carrying the ST-133B ST-133B Power Switch For the ST-133B, the power switch has been re-l ocated to the back of the Controller and is positioned above the power module. To eliminate unwanted light sources around optical se[...]