Leica DM750 manuel d'utilisation

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Un bon manuel d’utilisation

Les règles imposent au revendeur l'obligation de fournir à l'acheteur, avec des marchandises, le manuel d’utilisation Leica DM750. Le manque du manuel d’utilisation ou les informations incorrectes fournies au consommateur sont à la base d'une plainte pour non-conformité du dispositif avec le contrat. Conformément à la loi, l’inclusion du manuel d’utilisation sous une forme autre que le papier est autorisée, ce qui est souvent utilisé récemment, en incluant la forme graphique ou électronique du manuel Leica DM750 ou les vidéos d'instruction pour les utilisateurs. La condition est son caractère lisible et compréhensible.

Qu'est ce que le manuel d’utilisation?

Le mot vient du latin "Instructio", à savoir organiser. Ainsi, le manuel d’utilisation Leica DM750 décrit les étapes de la procédure. Le but du manuel d’utilisation est d’instruire, de faciliter le démarrage, l'utilisation de l'équipement ou l'exécution des actions spécifiques. Le manuel d’utilisation est une collection d'informations sur l'objet/service, une indice.

Malheureusement, peu d'utilisateurs prennent le temps de lire le manuel d’utilisation, et un bon manuel permet non seulement d’apprendre à connaître un certain nombre de fonctionnalités supplémentaires du dispositif acheté, mais aussi éviter la majorité des défaillances.

Donc, ce qui devrait contenir le manuel parfait?

Tout d'abord, le manuel d’utilisation Leica DM750 devrait contenir:
- informations sur les caractéristiques techniques du dispositif Leica DM750
- nom du fabricant et année de fabrication Leica DM750
- instructions d'utilisation, de réglage et d’entretien de l'équipement Leica DM750
- signes de sécurité et attestations confirmant la conformité avec les normes pertinentes

Pourquoi nous ne lisons pas les manuels d’utilisation?

Habituellement, cela est dû au manque de temps et de certitude quant à la fonctionnalité spécifique de l'équipement acheté. Malheureusement, la connexion et le démarrage Leica DM750 ne suffisent pas. Le manuel d’utilisation contient un certain nombre de lignes directrices concernant les fonctionnalités spécifiques, la sécurité, les méthodes d'entretien (même les moyens qui doivent être utilisés), les défauts possibles Leica DM750 et les moyens de résoudre des problèmes communs lors de l'utilisation. Enfin, le manuel contient les coordonnées du service Leica en l'absence de l'efficacité des solutions proposées. Actuellement, les manuels d’utilisation sous la forme d'animations intéressantes et de vidéos pédagogiques qui sont meilleurs que la brochure, sont très populaires. Ce type de manuel permet à l'utilisateur de voir toute la vidéo d'instruction sans sauter les spécifications et les descriptions techniques compliquées Leica DM750, comme c’est le cas pour la version papier.

Pourquoi lire le manuel d’utilisation?

Tout d'abord, il contient la réponse sur la structure, les possibilités du dispositif Leica DM750, l'utilisation de divers accessoires et une gamme d'informations pour profiter pleinement de toutes les fonctionnalités et commodités.

Après un achat réussi de l’équipement/dispositif, prenez un moment pour vous familiariser avec toutes les parties du manuel d'utilisation Leica DM750. À l'heure actuelle, ils sont soigneusement préparés et traduits pour qu'ils soient non seulement compréhensibles pour les utilisateurs, mais pour qu’ils remplissent leur fonction de base de l'information et d’aide.

Table des matières du manuel d’utilisation

  • Page 1

    Leica DM750 Manual[...]

  • Page 2

    Leica DM750 Manual 2 Published in Nov ember 2010 by: Leic a Microsystems (Schweiz) A G Industry Division Max Schmidheiny Strasse 201 CH-9435 Heerbrugg (Switzerland) Responsible for conten ts: Dietmar Gnass (Product Complianc e) Vincen t V accarelli (P roduct Management) In case of questions, please contact the hotline: mqm-hotline@leica-microsystem[...]

  • Page 3

    Leica DM750 Manual 3 Chapter ov erview Safety regulations 5 The Leica DM750 17 Ready! 20 Set! 30 Go! 43 Care of the Microsc ope 45 T r oubleshooting 48 Dimensions 51[...]

  • Page 4

    Leica DM750 Manual 4 Con tents Safety regulations 5 Safety concept 6 Symbols used in this instruction manual 7 Impor tant Notes 8 Intended Purpose of the Microscope 9 Instructions on use 10 Health risks and dangers of use 12 Information for the person r esponsible for the instrument 13 Care instructions 14 Accessories , maintenance and r epair 15 E[...]

  • Page 5

    Leica DM750 Manual 5 S afety r egula tions[...]

  • Page 6

    Leica DM750 Manual 6 Safety concept The individual modules of the Leica DM microscopy series include an in teractive CD-ROM with all relevant user manuals in several languages . Keep it in a safe place, and readily accessible t o the user . User manuals and updates are also a vailable for y ou to download and print from our web sit e at ww w .leica[...]

  • Page 7

    Leica DM750 Manual 7 Symbols used in this instruction manual W arning of a danger  This symbol indicates especially impor - tant information that is manda tory to read and observe. F ailure to comply can cause the following: Ϙ Hazards to personnel Ϙ F unctional disturbances or damaged inst - ruments W arning of hazardous electrical voltage [...]

  • Page 8

    Leica DM750 Manual 8 Impor tant Notes Description The Leica DM750 micr oscope meets today's state of the art of technology . Never theless, hazards may still arise during opera tion. The potential risks are described belo w .  Before installing , operating or using the instrument, it is mandatory to read this user manual. In particular , pl[...]

  • Page 9

    Leica DM750 Manual 9  The Leica DM750 micr oscope, f or which this instruction manual has been writ - ten, is intended f or biological routine and research applica tions. This includes exami - ning specimens taken from the human body for the purpose of gaining information about physiological or patholog ical conditions or inborn anomalies, or te[...]

  • Page 10

    Leica DM750 Manual 10 Instructions on use  The Leica DM750 micr oscope may be used only in closed rooms and must be placed on a solid substrate . The Leica DM750 micr oscope can be used in clean rooms without any pr oblems.  Always position the L eica DM750 microscope so that y ou can disconnect it from the pow er supply at any time . The pow[...]

  • Page 11

    Leica DM750 Manual 11 Instructions on use (continued)  The responsible L eica affiliate must be consulted whenever the instrumen t is altered , modified or used in conjunction with non-Leica components tha t are outside of the scope of this manual!  Unauthorized alterations t o the instru - ment or noncompliant use shall void all rights to a[...]

  • Page 12

    Leica DM750 Manual 12 Health risks and dangers of use Health risks  W orkplaces with microscopes facilitat e and improv e the viewing task, but they also impose high demands on the eyes and holding muscles of the user . Depending on the duration of uninterrupt ed work, asthenopia and musculoskeletal problems may oc cur . For this reason, appropr[...]

  • Page 13

    Leica DM750 Manual 13 Information f or the person responsible for the instrument Information for the person r esponsible for the instrument Ϙ Ensure that the L eica DM750 microscope is used only by qualified personnel. Ϙ Ensure that this user manual is alwa ys available at the L eica DM750 microscope . Ϙ Carry out regular inspections to make ce[...]

  • Page 14

    Leica DM750 Manual 14 Care instructions General instructions Ϙ Prot ect the Leica DM750 microscope against damp, v apors, acids, alkalis, and corrosiv e substances. Do not stor e chemi - cals in the vicinity . Ϙ Prot ect the Leica DM750 microscope fr om oil and grease . Never grease or oil mechani - cal parts or sliding sur faces. Ϙ F ollow the [...]

  • Page 15

    Leica DM750 Manual 15 Ac cessories , maintenanc e and repair Acc essories Only the following acc essories may be used with the Leica DM750 microsc ope: Ϙ The Leica ac cessories described in this user manual. Ϙ Other accessories, pro vided that these have been expressly appro ved by L eica as being technically safe in this cont ext. Maintenance Ϙ[...]

  • Page 16

    Leica DM750 Manual 16 Electric al data and ambient conditions  In order to maintain this condition and t o ensure safe opera tion, the user must follow the instructions and warnings contained in this instruction manual. Environmen t T emperature f or use +10 °C … +40 °C Storage temper ature -20 °C … +52 °C +50 °F to +104 °F Manipulatio[...]

  • Page 17

    Leica DM750 Manual 17 T he L eica DM750[...]

  • Page 18

    Leica DM750 Manual 18 Thank you f or purchasing the Leica DM750 Compound Microscope fr om Leica Microsys - tems. This model’ s exclusiv e design featur es and full range of accessories make it a truly versatile , high quality-instrument for educatio - nal and routine clinical applications .  The instrument is to be used only as described. Haza[...]

  • Page 19

    Leica DM750 Manual 19 Ϙ Carefully r emove the micr oscope and any components from the packing carton. Ϙ V erify that all components ar e intact. Ϙ Check the components against the planned configuration. Ϙ Optional items such as contrast ac cessories, camera adapters, camer as, and carrying cases are not shipped as part of the stan - dard equip[...]

  • Page 20

    Leica DM750 Manual 20 Ready![...]

  • Page 21

    Leica DM750 Manual 21 Substage Illumination  The Leica DM750 is a vailable with two types of Substage Illumination. Identify which type of Illumination you have , as this will be important to know later . T ype 1: Standard illumination Adjustable condenser cen tering with provided tool. T ype 2: Koehler illumination Adjustable condenser cent eri[...]

  • Page 22

    Leica DM750 Manual 22 T ools used Ϙ Allen key  There ar e two types of viewing tube. Identify which viewing tube you hav e: T ype 1: Leic a EZ Viewing T ube with integrated eyepieces . T ype 2: Standard viewing tube with separ ate eyepieces 1. Loosen the setscrew ( on top of the stand) using the Allen key provided . 2. Set the dovetail into the[...]

  • Page 23

    Leica DM750 Manual 23 2. Reinstall the Leica EZ Viewing Tube ont o the stand. 3. Tighten the captiv e wingscrew using the wrench included in the delivery package. Leica EZ Viewing T ube – integr at ed ey epieces  T o rotate the L eica EZ viewing tube, either loosen the set screw on the stand or replace the set scr ew with the (optional) captiv[...]

  • Page 24

    Leica DM750 Manual 24 Leica EZ Viewing T ube – integr at ed ey epieces (con t.)  Y ou can now safely r otate the L eica EZ viewing tube by loosening the captive thumbscrew , rotating the viewing tube, and tightening the captive thumbscr ew again.  The eyepieces ar e integra ted into the Leica EZ Viewing Tube and pr eset; there - fore , ther[...]

  • Page 25

    Leica DM750 Manual 25 Leica Standard Vie wing T ube – separated e yepiec es 2. Secure the eyepieces in the tubes by tighte - ning the silver screws a t the bottom of the tubes using a standard Phillips screw driver (not included in the delivery pack age).  The eyepieces will still r otate , but they will be captive in the eyetubes .  The st[...]

  • Page 26

    Leica DM750 Manual 26  If you wear eyeglasses f or microscope viewing, keep the rubber ey ecups folded down. If you do not wear ey eglasses, you ma y find it useful to unfold the rubber ey ecups in order to help block out ambient r oom light. Ey ec ups  If you have pur chased a standard microscope configur ation, you will notice that the ob[...]

  • Page 27

    Leica DM750 Manual 27 Installing Objectives Installing Objectives  When rotating the objective nosepiece , always use the knurled ring on the objective nosepiece. As you rotate the nosepiec e clockwise, attach the objectives by screwing them into the nosepiec e holes starting with the lowest magnification and advancing to the highest magnific [...]

  • Page 28

    Leica DM750 Manual 28 Installing the Substage Condenser Substage Condenser  The Leica DM750 has an open substage condenser mount, theref ore the conden - ser needs to be installed. 1. Move the specimen stage upwar ds as far as it will go by using the coarse focusing knob on the side of the microscope . 2. Move the c ondenser holder into the low [...]

  • Page 29

    Leica DM750 Manual 29 4. Push the substage condenser under the specimen stage into the holder by alig - ning the locating pin on the bottom of the condenser with the slot on the rear side of the fork. 5. Move the c ondenser into the highest posi - tion by using the condenser focusing knob on the left side of the stage mount. 6. Tighten the two sets[...]

  • Page 30

    Leica DM750 Manual 30 S et![...]

  • Page 31

    Leica DM750 Manual 31 W ork Surface  Always use y our microscope on a hard , stable sur face. Po wer C ord  If the power cor d is not already atta - ched, attach it secur ely to the back of the microscope . Setting the Illumination Intensity  Set the illumination to the lowest setting to start with, using the control - ler on the bottom le[...]

  • Page 32

    Leica DM750 Manual 32  If you have pur chased a standard L eica DM750 configuration, the condenser has already been pre-centered by L eica Microsys - tems. 1. Open the condenser aper ture by r otating the knurled ring on the condenser to the right.  Make sure that the condenser is in the highest position. 2. Write an "X" on a piece[...]

  • Page 33

    Leica DM750 Manual 33 Using the Condenser  The condenser is furnished with an iris diaphragm, which can be adjusted to match the effective numerical aperture of the objective 1. T o open and close this diaphragm, simply turn the knurled ring on the condenser to the right or left so that the line on the ring is aligned with the objective magni?[...]

  • Page 34

    Leica DM750 Manual 34 1. P osition a specimen slide on the specimen stage by sliding it under the slide grips.  Slide grips hold the slide in place. 2. Using the X/Y -stage control , position the specimen slide such that a part of the speci - men is under the objective used. Prepar e to Vie w a Specimen Slide Slide grips[...]

  • Page 35

    Leica DM750 Manual 35 1. Rotate the objective nosepiece (using the knurled ring) in such a way that the objec - tive with the low est magnification level is rotated in to the working position. 2. Move the specimen stage upwar ds by turning the coarse focusing knob as far as it will go to the maximum position. 3. Look into the eyepieces and adjust [...]

  • Page 36

    Leica DM750 Manual 36 Adjust the ey etubes 1. Adjust the tubes to y our interpupillary distance. F old or unfold the eyetubes to decrease or increase the distanc e between the eyepieces until y ou see one illuminated circle.  The viewing tubes maintain a constant tube length for all interpupillary settings. This means that a change of interpupil[...]

  • Page 37

    Leica DM750 Manual 37  If you are comf ortable wearing your corrective lenses (con tact lenses or eyeglasses) for micr oscope viewing, lea ve them on and your adjustments will be minimal. 2. Using the fine focus adjusting knob , focus on the specimen while looking through only one of the eyepieces (when using one eyepiece that is f ocusable and[...]

  • Page 38

    Leica DM750 Manual 38  If your Leica DM750 is fitted with a field diaphr agm for Koehler Illu - mination, use the following pr ocedure t o assure condenser cen te - ring and focus. 1. Connect the Koehler field diaphragm to the base of the microscope so that the diaphragm leav es are in the field of view when you look through the eyepiec es. [...]

  • Page 39

    Leica DM750 Manual 39 3. T urn the condenser centering wingscrews simultaneously to cen ter the image of the field diaphragm. 4. Open the field diaphragm until the diaphrag m leaves ar e just outside of the field of view . Koehler C onfiguration (cont .)[...]

  • Page 40

    Leica DM750 Manual 40 1. Search for the area on the specimen slide that you want t o study . 2. Move the specimen stage down t o the lowest position using the coarse adjust - ment knob. 3. Add a drop of immersion oil fr om Leica to the area of the specimen slide y ou are studying. 4. Rotate the oil immersion objective (the objective labeled "O[...]

  • Page 41

    Leica DM750 Manual 41 Oil Immersion T echnique (cont .) 5. Slowly move the specimen stage up wards using the coarse adjustment knob until the oil drop on the slide comes int o direct contact with the lens of the oil immersion objective. 6. Hold the k nurled ring on the objective turret and swing the objective back and forth to eliminate air bubbles[...]

  • Page 42

    Leica DM750 Manual 42  The Leica DM750 is equipped with a time delay shutoff capability , which automati - cally turns the illumination off after 2 hours of no changes in the intensity control . Ϙ All four-position nosepiece stands ha ve the time delay shutoff enabled as a default (mostly educational applications). Ϙ All five-position nose[...]

  • Page 43

    Leica DM750 Manual 43 Go![...]

  • Page 44

    Leica DM750 Manual 44 Now all you need to do is change objectives , set the condenser aperture (and field diaphragm if you hav e a DM750 Koehler Stand) appropriat ely for the objective magnification y ou are using, and enjoy the view! Ready! Set! Go![...]

  • Page 45

    Leica DM750 Manual 45 Car e of the M icrosc ope[...]

  • Page 46

    Leica DM750 Manual 46 General maintenanc e Storage and handling  Always use both hands to carry the microscope . There is a handle on the back of the microscope and an under cut in the front for this purpose . Ϙ The cord wr ap allows you t o wrap the cord in such a way that only the length you need is extended. Ϙ Keep all optical components cl[...]

  • Page 47

    Leica DM750 Manual 47 Ϙ No part of the microscope is quite so vulne - rable to collecting dirt, dust, and oil as the front lens of the objective. Whenever you encounter lack of contr ast, cloudiness or poor definition, carefully check the condi - tion of the front lens with a magnifier . Ϙ The lower mag nification objectives have fairly large [...]

  • Page 48

    Leica DM750 Manual 48 T r oubleshooting[...]

  • Page 49

    Leica DM750 Manual 49 T roubleshooting Stand The microscope does not r espond. Make sure that the socket has v oltage. Check the cable connections. Make sure that the stand is connected to the po wer supply . Check whether the fuse is defective and replace it if nec essary (see page 16). Focus The specimen cannot be brought in to focus. Use the cor[...]

  • Page 50

    Leica DM750 Manual 50 T roubleshooting (c ontinued) Polariza tion contrast cannot be adjusted . Bring the polarizer and analyzer int o cross position until they reach maximum darkness (without specimen). Phase contrast Phase contrast cannot be adjusted . The specimen is too thick, too thin, or too brightly stained . Refractive indexes of the mounti[...]

  • Page 51

    Leica DM750 Manual 51 Dimensions[...]

  • Page 52

    Leica DM750 Manual 52[...]

  • Page 53

    Leica DM750 Manual 53[...]

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    Leica DM750 Manual 54[...]