Leica DM750 Bedienungsanleitung

Leica DM750 Manual[...]

Leica DM750 Manual 2 Published in Nov ember 2010 by: Leic a Microsystems (Schweiz) A G Industry Division Max Schmidheiny Strasse 201 CH-9435 Heerbrugg (Switzerland) Responsible for conten ts: Dietmar Gnass (Product Complianc e) Vincen t V accarelli (P roduct Management) In case of questions, please contact the hotline: mqm-hotline@leica-microsystem[...]

Leica DM750 Manual 3 Chapter ov erview Safety regulations 5 The Leica DM750 17 Ready! 20 Set! 30 Go! 43 Care of the Microsc ope 45 T r oubleshooting 48 Dimensions 51[...]

Leica DM750 Manual 4 Con tents Safety regulations 5 Safety concept 6 Symbols used in this instruction manual 7 Impor tant Notes 8 Intended Purpose of the Microscope 9 Instructions on use 10 Health risks and dangers of use 12 Information for the person r esponsible for the instrument 13 Care instructions 14 Accessories , maintenance and r epair 15 E[...]

Leica DM750 Manual 5 S afety r egula tions[...]

Leica DM750 Manual 6 Safety concept The individual modules of the Leica DM microscopy series include an in teractive CD-ROM with all relevant user manuals in several languages . Keep it in a safe place, and readily accessible t o the user . User manuals and updates are also a vailable for y ou to download and print from our web sit e at ww w .leica[...]

Leica DM750 Manual 7 Symbols used in this instruction manual W arning of a danger  This symbol indicates especially impor - tant information that is manda tory to read and observe. F ailure to comply can cause the following: Ϙ Hazards to personnel Ϙ F unctional disturbances or damaged inst - ruments W arning of hazardous electrical voltage [...]

Leica DM750 Manual 8 Impor tant Notes Description The Leica DM750 micr oscope meets today's state of the art of technology . Never theless, hazards may still arise during opera tion. The potential risks are described belo w .  Before installing , operating or using the instrument, it is mandatory to read this user manual. In particular , pl[...]

Leica DM750 Manual 9  The Leica DM750 micr oscope, f or which this instruction manual has been writ - ten, is intended f or biological routine and research applica tions. This includes exami - ning specimens taken from the human body for the purpose of gaining information about physiological or patholog ical conditions or inborn anomalies, or te[...]

Leica DM750 Manual 10 Instructions on use  The Leica DM750 micr oscope may be used only in closed rooms and must be placed on a solid substrate . The Leica DM750 micr oscope can be used in clean rooms without any pr oblems.  Always position the L eica DM750 microscope so that y ou can disconnect it from the pow er supply at any time . The pow[...]

Leica DM750 Manual 11 Instructions on use (continued)  The responsible L eica affiliate must be consulted whenever the instrumen t is altered , modified or used in conjunction with non-Leica components tha t are outside of the scope of this manual!  Unauthorized alterations t o the instru - ment or noncompliant use shall void all rights to a[...]

Leica DM750 Manual 12 Health risks and dangers of use Health risks  W orkplaces with microscopes facilitat e and improv e the viewing task, but they also impose high demands on the eyes and holding muscles of the user . Depending on the duration of uninterrupt ed work, asthenopia and musculoskeletal problems may oc cur . For this reason, appropr[...]

Leica DM750 Manual 13 Information f or the person responsible for the instrument Information for the person r esponsible for the instrument Ϙ Ensure that the L eica DM750 microscope is used only by qualified personnel. Ϙ Ensure that this user manual is alwa ys available at the L eica DM750 microscope . Ϙ Carry out regular inspections to make ce[...]

Leica DM750 Manual 14 Care instructions General instructions Ϙ Prot ect the Leica DM750 microscope against damp, v apors, acids, alkalis, and corrosiv e substances. Do not stor e chemi - cals in the vicinity . Ϙ Prot ect the Leica DM750 microscope fr om oil and grease . Never grease or oil mechani - cal parts or sliding sur faces. Ϙ F ollow the [...]

Leica DM750 Manual 15 Ac cessories , maintenanc e and repair Acc essories Only the following acc essories may be used with the Leica DM750 microsc ope: Ϙ The Leica ac cessories described in this user manual. Ϙ Other accessories, pro vided that these have been expressly appro ved by L eica as being technically safe in this cont ext. Maintenance Ϙ[...]

Leica DM750 Manual 16 Electric al data and ambient conditions  In order to maintain this condition and t o ensure safe opera tion, the user must follow the instructions and warnings contained in this instruction manual. Environmen t T emperature f or use +10 °C … +40 °C Storage temper ature -20 °C … +52 °C +50 °F to +104 °F Manipulatio[...]

Leica DM750 Manual 17 T he L eica DM750[...]

Leica DM750 Manual 18 Thank you f or purchasing the Leica DM750 Compound Microscope fr om Leica Microsys - tems. This model’ s exclusiv e design featur es and full range of accessories make it a truly versatile , high quality-instrument for educatio - nal and routine clinical applications .  The instrument is to be used only as described. Haza[...]

Leica DM750 Manual 19 Ϙ Carefully r emove the micr oscope and any components from the packing carton. Ϙ V erify that all components ar e intact. Ϙ Check the components against the planned configuration. Ϙ Optional items such as contrast ac cessories, camera adapters, camer as, and carrying cases are not shipped as part of the stan - dard equip[...]

Leica DM750 Manual 20 Ready![...]

Leica DM750 Manual 21 Substage Illumination  The Leica DM750 is a vailable with two types of Substage Illumination. Identify which type of Illumination you have , as this will be important to know later . T ype 1: Standard illumination Adjustable condenser cen tering with provided tool. T ype 2: Koehler illumination Adjustable condenser cent eri[...]

Leica DM750 Manual 22 T ools used Ϙ Allen key  There ar e two types of viewing tube. Identify which viewing tube you hav e: T ype 1: Leic a EZ Viewing T ube with integrated eyepieces . T ype 2: Standard viewing tube with separ ate eyepieces 1. Loosen the setscrew ( on top of the stand) using the Allen key provided . 2. Set the dovetail into the[...]

Leica DM750 Manual 23 2. Reinstall the Leica EZ Viewing Tube ont o the stand. 3. Tighten the captiv e wingscrew using the wrench included in the delivery package. Leica EZ Viewing T ube – integr at ed ey epieces  T o rotate the L eica EZ viewing tube, either loosen the set screw on the stand or replace the set scr ew with the (optional) captiv[...]

Leica DM750 Manual 24 Leica EZ Viewing T ube – integr at ed ey epieces (con t.)  Y ou can now safely r otate the L eica EZ viewing tube by loosening the captive thumbscrew , rotating the viewing tube, and tightening the captive thumbscr ew again.  The eyepieces ar e integra ted into the Leica EZ Viewing Tube and pr eset; there - fore , ther[...]

Leica DM750 Manual 25 Leica Standard Vie wing T ube – separated e yepiec es 2. Secure the eyepieces in the tubes by tighte - ning the silver screws a t the bottom of the tubes using a standard Phillips screw driver (not included in the delivery pack age).  The eyepieces will still r otate , but they will be captive in the eyetubes .  The st[...]

Leica DM750 Manual 26  If you wear eyeglasses f or microscope viewing, keep the rubber ey ecups folded down. If you do not wear ey eglasses, you ma y find it useful to unfold the rubber ey ecups in order to help block out ambient r oom light. Ey ec ups  If you have pur chased a standard microscope configur ation, you will notice that the ob[...]

Leica DM750 Manual 27 Installing Objectives Installing Objectives  When rotating the objective nosepiece , always use the knurled ring on the objective nosepiece. As you rotate the nosepiec e clockwise, attach the objectives by screwing them into the nosepiec e holes starting with the lowest magnification and advancing to the highest magnific [...]

Leica DM750 Manual 28 Installing the Substage Condenser Substage Condenser  The Leica DM750 has an open substage condenser mount, theref ore the conden - ser needs to be installed. 1. Move the specimen stage upwar ds as far as it will go by using the coarse focusing knob on the side of the microscope . 2. Move the c ondenser holder into the low [...]

Leica DM750 Manual 29 4. Push the substage condenser under the specimen stage into the holder by alig - ning the locating pin on the bottom of the condenser with the slot on the rear side of the fork. 5. Move the c ondenser into the highest posi - tion by using the condenser focusing knob on the left side of the stage mount. 6. Tighten the two sets[...]

Leica DM750 Manual 30 S et![...]

Leica DM750 Manual 31 W ork Surface  Always use y our microscope on a hard , stable sur face. Po wer C ord  If the power cor d is not already atta - ched, attach it secur ely to the back of the microscope . Setting the Illumination Intensity  Set the illumination to the lowest setting to start with, using the control - ler on the bottom le[...]

Leica DM750 Manual 32  If you have pur chased a standard L eica DM750 configuration, the condenser has already been pre-centered by L eica Microsys - tems. 1. Open the condenser aper ture by r otating the knurled ring on the condenser to the right.  Make sure that the condenser is in the highest position. 2. Write an "X" on a piece[...]

Leica DM750 Manual 33 Using the Condenser  The condenser is furnished with an iris diaphragm, which can be adjusted to match the effective numerical aperture of the objective 1. T o open and close this diaphragm, simply turn the knurled ring on the condenser to the right or left so that the line on the ring is aligned with the objective magni?[...]

Leica DM750 Manual 34 1. P osition a specimen slide on the specimen stage by sliding it under the slide grips.  Slide grips hold the slide in place. 2. Using the X/Y -stage control , position the specimen slide such that a part of the speci - men is under the objective used. Prepar e to Vie w a Specimen Slide Slide grips[...]

Leica DM750 Manual 35 1. Rotate the objective nosepiece (using the knurled ring) in such a way that the objec - tive with the low est magnification level is rotated in to the working position. 2. Move the specimen stage upwar ds by turning the coarse focusing knob as far as it will go to the maximum position. 3. Look into the eyepieces and adjust [...]

Leica DM750 Manual 36 Adjust the ey etubes 1. Adjust the tubes to y our interpupillary distance. F old or unfold the eyetubes to decrease or increase the distanc e between the eyepieces until y ou see one illuminated circle.  The viewing tubes maintain a constant tube length for all interpupillary settings. This means that a change of interpupil[...]

Leica DM750 Manual 37  If you are comf ortable wearing your corrective lenses (con tact lenses or eyeglasses) for micr oscope viewing, lea ve them on and your adjustments will be minimal. 2. Using the fine focus adjusting knob , focus on the specimen while looking through only one of the eyepieces (when using one eyepiece that is f ocusable and[...]

Leica DM750 Manual 38  If your Leica DM750 is fitted with a field diaphr agm for Koehler Illu - mination, use the following pr ocedure t o assure condenser cen te - ring and focus. 1. Connect the Koehler field diaphragm to the base of the microscope so that the diaphragm leav es are in the field of view when you look through the eyepiec es. [...]

Leica DM750 Manual 39 3. T urn the condenser centering wingscrews simultaneously to cen ter the image of the field diaphragm. 4. Open the field diaphragm until the diaphrag m leaves ar e just outside of the field of view . Koehler C onfiguration (cont .)[...]

Leica DM750 Manual 40 1. Search for the area on the specimen slide that you want t o study . 2. Move the specimen stage down t o the lowest position using the coarse adjust - ment knob. 3. Add a drop of immersion oil fr om Leica to the area of the specimen slide y ou are studying. 4. Rotate the oil immersion objective (the objective labeled "O[...]

Leica DM750 Manual 41 Oil Immersion T echnique (cont .) 5. Slowly move the specimen stage up wards using the coarse adjustment knob until the oil drop on the slide comes int o direct contact with the lens of the oil immersion objective. 6. Hold the k nurled ring on the objective turret and swing the objective back and forth to eliminate air bubbles[...]

Leica DM750 Manual 42  The Leica DM750 is equipped with a time delay shutoff capability , which automati - cally turns the illumination off after 2 hours of no changes in the intensity control . Ϙ All four-position nosepiece stands ha ve the time delay shutoff enabled as a default (mostly educational applications). Ϙ All five-position nose[...]

Leica DM750 Manual 43 Go![...]

Leica DM750 Manual 44 Now all you need to do is change objectives , set the condenser aperture (and field diaphragm if you hav e a DM750 Koehler Stand) appropriat ely for the objective magnification y ou are using, and enjoy the view! Ready! Set! Go![...]

Leica DM750 Manual 45 Car e of the M icrosc ope[...]

Leica DM750 Manual 46 General maintenanc e Storage and handling  Always use both hands to carry the microscope . There is a handle on the back of the microscope and an under cut in the front for this purpose . Ϙ The cord wr ap allows you t o wrap the cord in such a way that only the length you need is extended. Ϙ Keep all optical components cl[...]

Leica DM750 Manual 47 Ϙ No part of the microscope is quite so vulne - rable to collecting dirt, dust, and oil as the front lens of the objective. Whenever you encounter lack of contr ast, cloudiness or poor definition, carefully check the condi - tion of the front lens with a magnifier . Ϙ The lower mag nification objectives have fairly large [...]

Leica DM750 Manual 48 T r oubleshooting[...]

Leica DM750 Manual 49 T roubleshooting Stand The microscope does not r espond. Make sure that the socket has v oltage. Check the cable connections. Make sure that the stand is connected to the po wer supply . Check whether the fuse is defective and replace it if nec essary (see page 16). Focus The specimen cannot be brought in to focus. Use the cor[...]

Leica DM750 Manual 50 T roubleshooting (c ontinued) Polariza tion contrast cannot be adjusted . Bring the polarizer and analyzer int o cross position until they reach maximum darkness (without specimen). Phase contrast Phase contrast cannot be adjusted . The specimen is too thick, too thin, or too brightly stained . Refractive indexes of the mounti[...]

Leica DM750 Manual 51 Dimensions[...]

Leica DM750 Manual 52[...]

Leica DM750 Manual 53[...]

Leica DM750 Manual 54[...]