SSI America 90-2581 REV B Bedienungsanleitung

Binary Solvent Delivery Module Dual Series-III Pumps with Serial PC and Voltage Control Operator's Manual 90-2581 rev B Scientific Systems, Inc. 349 N. Science Park Road State College, P 1 6803[...]

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1. INTRODUCTION This operator's manual contains inform ation needed to install, operate, and perform minor m aintenance on the Binary Solvent Delivery Module. The figures below are for reference of items described in the manual. Left Drawer Right Drawer Control Ke yp ads Outlet Filters Prime Purge V alves “T” With Outlet Power Switch RS-23[...]

1-2 1.1 Description of the Binary Solvent Delivery Module The system consists of two Series III high performance m etering pumps, in a compact package with pulse dam pener and an off-line pressure transducer. The pumps use a drawer system for easy access to internal components for troubleshooting & service. These features allow for: f Fast &[...]

Flow adjustment in 0.01 ml increm ents, from 0.01 to 10.00 ml/min with a precision of 0.2% RSD (10m L/min heads) • Microprocessor advanced control • Digital stepper motor design to prevent flow rate drift over time and temperature • Back panel RS232 serial communications port for complete control and status monitoring • Remote analog inputs[...]

1-4 1.1.4 Self-Flush and Seal Life It is recommended that the Self Flush feature be used to im prove seal life in a number of applications. In particular, (as stated above) if pumping Buffers, Acids/Bases or any inorganic solution near saturation, the pump should utilize the Self Flush feature. With every piston stroke, an extremely thin film of so[...]

1-5 1.2 Specifications for the Bi nary Solvent Delivery Modules Flow Rates ............ 0.000 to 5.000 mL/min for 5mL/min heads 0.00 to 10.00 mL/min for 10mL/min heads Pressure ................ 0 to 6,000 p.s.i. for SS pump heads, 0 to 5,000 p.s.i. for PEEK™ heads, Pressure Accuracy… . ± 1% of full-scale pressure Pressure Zero Offset. . ± 2 p[...]

3-1 2. INSTALLATION 2.1 Unpacking and Inspection Prior to opening the shipping container, inspect it for damage or evidence of mishandling. If it has been damaged or m ishandled, notify the carrier before opening the container. Once the container is opened, inspect the contents for damage. Any damage should be reported to the carrier immediately. S[...]

3-2 SELF-FLUSH Connect wash solution inlet and outlet tubing (opaque) to the flush housing as shown. Make sure ferrule is in the correct position. Screw syringe on luer fitting. Insert wash outlet tubing end into clear adapter. PUMP Connect pump inlet tubing as shown. Make sure ferrule is in the correct position. Ensure inlet line filter is submers[...]

3-3 2.4 Electrical Connection The system utilizes Universal Switching Power Supply, and will accept voltages from 90 – 260 VAC, 50-60 Hz. WARNING: Do not bypass the safety ground connection as a serious shock hazard could result. 2.5 Solvent Preparation Proper solvent preparation will prevent a great number of pum ping problems. The most com mon [...]

3-4 2.5.2 Cavitation Cavitation occurs when inlet conditions restrict the flow of solvent and vapor bubbles are formed during the inlet stroke. The key to preventing cavitation is to reduce inlet restrictions. The most comm on causes of inlet restrictions are crimped inlet lines and plugged inlet filters. Inlet lines with tubing longer than 48"[...]

3-5 2.6.2 Self-Flush Solution Self-flush heads require 250-500 mL of flushing solution. See section 1.1.4 for self-flush solution recommendations. A pH indicator that will indicate the concentration of salts in the solution is recommended as a rem inder to change the solution. This flush solution should be replaced with a fresh solution weekly to a[...]

2.7 Preparation for Storage or Shipping 2.7.1 Isopropanol Flush Disconnect the outlet tubing from the pump. Place the inlet f ilter in isopropanol. Use a syringe to draw a minimum of 50 ml through the pump. Pump a m inimum of 5 m l of isopropanol to exit. Leave the inlet tubing connected to the pump. Place the inlet filter in a small plastic bag an[...]

3-7 3. OPERATION 3.1 Pump Front Panel Controls and Indicators 3.1.1 Control Panel 3.1.1.1 Digital Display The 3-digit display shows the pump flow rate (mL/m in), system pressure (psi), or the set upper or lower pressure limit (psi) when operating. Choice of display is selected with the MODE key. 3.1.1.2 Digital Display Pump Keypad ∆ When pressed,[...]

3-8 3.1.1.3 Status LEDs Q ml/min When lit, the d ig ital d isplay shows flow rate in mL/min. Q PSI When lit, the digital display show s system pressure in psi. Q HI PR When lit, the display shows the use r-set upper pressure limit in psi. Q LO PR When lit, the d isp lay sh ows the user-set lower pr essure limit in psi. Q RUN Lights to indicate t ha[...]

Align Refill Switch Mode: The signal that initiates the refill phase can be displayed during power-up by pressing and holding the PRIME and the UP-ARROW buttons when the power is switched on. Release the buttons when the display displays "rFL". When the slotted disk allows the light beam to pass from the emitter to the detector on the slo[...]

4-1 4. THEORY OF OPERATION 4.1 Pump Mechanical Operation 4.1.1 Liquid System Flow Path The flow path of the Binary Solvent Delivery Module starts at the inlet reservoir filter passes through the inlet check valve, then through the pump head, and finally exits through the outlet check valve. 4.1.2 Pump Cycle The pump cycle consists of two phases, th[...]

4-2 4.2 Electronic Control 4.2.1 Microprocessor Control The pump is controlled by hybrid microprocessor circuitry which (1) provides control signals to the motor drive circuitry, (2) interfaces with the keyboard/display, (3) receives signals from the refill flag, and (4) provides external input/output (RS-232) interfacing. Firmware programming is s[...]

4-3 Motor Stall Detector press the DOWN-ARROW button and the display will display "OFF". To exit this mode and store the current setting in non-volatile memory, press the RUN/STOP button. The Motor Stall Detector uses a timer to determine if the camshaft has stopped turning or if the refill switch is defective. The timer begins timing aft[...]

7-1 5. MAINTENANCE Cleaning and minor repairs of the Binary pump can be perform ed as outlined below. NOTE: Lower than normal pressu re, pressure variations, or leaks in the pumping system can all indicate possible problems with the piston seal, piston, or check valves. Piston seal replacement coul d be necessary after 1,000 hours of running time. [...]

7-2 CAUTION: Check that the All en nuts at the front of the pump head are secure before re moving any tubing from the pump head. 3. Remove the inlet line from the inlet check valve. 4. Remove the outlet line from the outlet check valve. 5. Remove inlet and outlet self-flush check valves. 6. Momentarily turn ON the Binary pump and quickly turn OFF t[...]

7-3 PUM P HEA D OU T L ET C HECK V ALV E IN LET C H EC K V ALVE SE AL SEA L BA C KU P WASH ER O-RING OUTLET FLUS HING CH ECK VALV E INL ET F LUSHI NG CHECK VAL VE SELF-FLUSH HOUSIN G SELF- FLUSH SEAL SE AL B AC KU P PLAT E PIS TO N Figure 5-1. Stainless Steel Self-Flushing Pump Head Assembly PUM P H EA D OUT L ET CH ECK VA L VE INLET CHECK VALV E S[...]

7-4 INLE T F LUSHI NG CHECK VA LVE SEAL SEAL BAC KUP W ASHER SELF FLUS H HOU SIN G SEL F- FLU SH S E AL SEA L BACK UP PLATE PISTO N OUTLE T F LUS HING CHECK VALV E O-RING OUT LET CHE CK VA LV E PUMP HEAD IN LET CH ECK VA L VE Figure 5-3. Bioclean (PEEK™) Self-Flushing Pump Head Assembly O-RING SE AL BACK UP WASHE R SEAL SELF-FLUS H HOUSING SE AL [...]

7-5 5.2.2 Cleaning the Pump Head Assembly NOTE: If you choose to remove the piston seal or self-flush seals, you should have a new se t on hand to install after cleaning. It is not recommended that you reinstall used piston or self-flush seals since they are likely to be scratched and damaged during re moval and would not provide a reliable seal if[...]

7-6 3. If the check valves have been removed, tighten the check valves on stainless steel pumps to 75 inch-pounds or enough to seal at maxim um pressure. Do not exceed maximum torque. For Bioclean (PEEK™) pumps, tighten each check valve firmly by hand. Do not go ¼ turn past finger tight. NOTE: The inlet check valve has a larger opening (1/4"[...]

7-7 F L O W RI NG OUTL E T CH EC K VA L VE (PE EK ) F L O W RING INLE T CHECK VA LVE (PEEK) F L O W CROSS BALL RETAINER INLE T SEL F-FLU SH CH EC K VALVE F L O W TRANSPARENT W ASHER OUTLET SELF-FLU SH C H ECK VA LVE SINGLE INLET H OLE INLET CHECK VAL VE (S S) MULT IPL E OUTL ET HO L ES OUT LET CHECK V ALV E (S S) F L O W F L O W Figure 5-5. Check V[...]

7-8 5.2.3.1 Removing the Seals 1. Remove the pump head as described in Section 5.2.1. Use caution so as not to damage the sapphire piston. 2. Insert the flanged end of the seal insertion/removal tool into the seal cavity on the pump head. Tilt it slightly so that flange is under the seal and pull out the seal. CAUTION: Using any other “tool” wi[...]

7-9 2. Place a self-flush replacement seal on the seal insertion/removal tool so that the spring in the seal is visible when the seal is on the tool. As in the previous step, insert the tool and seal into the seal cavity on the flushing housing, taking care to line up the seal with the cavity, and then withdraw the tool. When the seal is fully inse[...]

7-10 5.2.5 Replacing the Pump Head CAUTION: The sapphire piston is fragile. Twisting the pump head during replacement can cause the piston to break. Closely follow instructions during head removal and replacement to avoid breakage. 1. Make sure that the inlet valve is on the bottom and the outlet valve is on the top. Carefully align the self-flush [...]

7-11 5.4 Check Valve Cleaning and Replacement Many check valve problems are the result of small particles interfering with the operation of the check valve. As a result, most problems can be solved by pumping a strong solution of liquid, laboratory grade detergent through the check valves at a rate of 1 mL/min for one hour. After washing with deter[...]

7-12 rebuilt. Do not attempt to refill or refurbish the pulse damper until you have a refurbishing kit. Instructions are furnished with the kit. 5.5.3 Pulse Damper Installation 1. Position the pulse damper, aligning it with the four mounting holes in the bottom of the cabinet. The pressure transducer should be pointed toward the rear of the cabinet[...]

7-13 10. If storing the pump for more than 12 hours fill each pum p head with 100% isopropanol then seal the inlets and outlet with the supplied plugs. 5.7 Lubrication The Binary pump has modest lubrication requirem ents. The bearings in the pump housing and piston carrier are permanently lubricated and require no maintenance. A small dab of light [...]

7-14 The Pump mechanism , Control Boards, & Pulse Dampener (with Pressure Transducer) are accessible by removing pump drawer.[...]

7-15 5.9 Fuse Replacement Three fuses protect the Binary Solvent Delivery Module. Two of the fuses are located in the power entry module at the rear of the cabinet and are in series with the AC input line. The other fuse is located on the circuit board and is in series with the 24 VDC supply. Troubleshooting the fuses is straightforward. If the pow[...]

7-16 5.10 Battery Replacement (If applicable) See attached photo for battery option. Depending on the version of drive board assembly installed, the board may not have a battery. If the printed circuit board does not have a battery, it is designed with circuitry that does not require a battery backup and you should disregard the following instructi[...]

7-17 6. PROBLEM SOLVING Quick Guide to Problem Solving You Notice 1. Uneven pressure trace. 2. Pressure drops. 3. No flow out the outlet check valve. This May Mean 1. Bubble in check valve. 2. Leaks in system. 3. Dirty check valve. 4. Bad check valve. Possible Cause 1. Solvent not properly degassed. 2. Fittings are not tight. 3. Mobile phase not pr[...]

7-18 7. LIST OF REPLACEMENT PARTS BINARY, STAINLESS STEEL 880201 Seal Kit, Aqueous, 5mL 880202 Seal Kit, Organic, 5mL 880203 Seal Kit, Aqueous, 10mL 880204 Seal Kit, Organic, 10mL 880407 Check Valve Kit – Stainless Steel 880721 Replacement Inlet Filter Elements (Package of 2) 880651 Prime Purge Valve Rebuild Kit – Stainless Steel 880353 Series [...]

7-19 BINARY, PEEK 880201 Seal Kit, Aqueous, 5mL 880202 Seal Kit, Organic, 5mL 880203 Seal Kit, Aqueous, 10mL 880204 Seal Kit, Organic, 10mL 880408 Check Valve Kit - PEEK 880721 Replacement Inlet Filter Elements (Package of 2) 880652 Prime Purge Valve Rebuild Kit - PEEK 880353 Series II-IV, Binary Piston, 5mL 880354 Series II-IV, Binary Piston, 10mL[...]

A-1 APPENDIX A A.1 Rear Panel Serial Communications Port - Pump An RS-232C modular jack is provided on the back panel. A computer, with appropriate software, can be used as a remote controlling device for pump operation via this connection. A.1.1 Hardware Implementation The REMOTE INPUT serial communications port is configured for 9600 baud, 8 data[...]

A-2 output is at a low logic level. A low logic level is -3.0 to -15 volts and a high logic level is 3.0 to 15 volts. A.1.3 Command Interpreter The pump’s high-level comm and interpreter receives and responds to command packets. The pum p will not send a message except when prompted, and it will send a response to every valid comm and as describe[...]

A-3 CS OK,x.xx,y,z,PSI,w,v,u/ (x.xxx, xx.xx, or xxx.x) (y, yy, yyy, or yyyy) (z, zz, zzz, or zzzz) Reads the current pump setup, where: x.xxx, xx.xx, or xxx.x = Flow rate in mL/min y, yy, yyy, or yyyy = Upper pressure limit z, zz, zzz, or zzzz = Lower pressure limit PSI = Units (PSI, ATM, MPA, BAR, or KGC) w = Pump head size (0 = standard, 1 = macr[...]

A-4 PI OK,a.aa,b,c,d,e,f,g,h,i,j,k,l, m,n,o,p,q/ (a.aaa, a.aa, aa.aa, or aa.a) (c or cc) Reads the current pump setup, where: a.aaa, a.aa, aa.aa, or aa.a = Flow rate in mL/min b = Run status (0 = stopped, 1 = running) c or cc = Pressure compensation d = Pump head type (see RH command) e = Pressure Board present = 0; otherwise 1 f = External control[...]

A-5 the EVENT 1 terminal and the EVENT 2 and EVENT 3 terminals is affected. EVENT 2 is Normally Closed (connected to EVENT 1) until a fault occurs and then opens. EVENT 3 is Normally Open (not connected to EVENT 1) until a fault occurs and then closes. A.2.1.1 Upper and Lo wer Pressure Limit Range (pump A only) The pressure sensing transducer provi[...]

A-6 Pin Function 10 VOLTAGE COM 9 VOLTAGE IN 8 FREQ IN 7 ENABLE IN 6 PUMP-RUN 5 PUMP-STOP 4 No connection 3 No connection 2 No connection 1 COM A.2.3 General Information on Run, Stop, and Enable Inputs The PUMP-RUN, PUMP-STOP, and ENABLE IN inputs operate from an internal 5 VDC source and each one draws approximately 0.008 amps when connected to CO[...]

A-7 A.2.4 Enable Input When activated (ENABLE IN is at a low logic level), the ENABLE IN input disables flowrate control on the front panel and enables flowrate control on the back panel. A.2.5 General Informati on on Voltage and Frequency Inputs Special programming and circuitry allows this pum p to be operated remotely with the flowrate controlle[...]

APPENDIX B EZ GRIP FITTING GUIDE A-8[...]

A-9 Scientific Systems Inc. Warranty Statement Scientific Systems, Inc. (SSI) warrants that instruments or equipm ent manufactured by it for a period thirty-six (36) months from date of shipm ent to customer, against defects in materials and workmanship under normal installation, use and m aintenance. Expendable items and physical damage caused by [...]

A-10 IMPLIED, INCLUDING WITHOUT LIMITATION ANY WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE.[...]