Dynex 91000051 manual

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Buen manual de instrucciones

Las leyes obligan al vendedor a entregarle al comprador, junto con el producto, el manual de instrucciones Dynex 91000051. La falta del manual o facilitar información incorrecta al consumidor constituyen una base de reclamación por no estar de acuerdo el producto con el contrato. Según la ley, está permitido adjuntar un manual de otra forma que no sea en papel, lo cual últimamente es bastante común y los fabricantes nos facilitan un manual gráfico, su versión electrónica Dynex 91000051 o vídeos de instrucciones para usuarios. La condición es que tenga una forma legible y entendible.

¿Qué es un manual de instrucciones?

El nombre proviene de la palabra latina “instructio”, es decir, ordenar. Por lo tanto, en un manual Dynex 91000051 se puede encontrar la descripción de las etapas de actuación. El propósito de un manual es enseñar, facilitar el encendido o el uso de un dispositivo o la realización de acciones concretas. Un manual de instrucciones también es una fuente de información acerca de un objeto o un servicio, es una pista.

Desafortunadamente pocos usuarios destinan su tiempo a leer manuales Dynex 91000051, sin embargo, un buen manual nos permite, no solo conocer una cantidad de funcionalidades adicionales del dispositivo comprado, sino también evitar la mayoría de fallos.

Entonces, ¿qué debe contener el manual de instrucciones perfecto?

Sobre todo, un manual de instrucciones Dynex 91000051 debe contener:
- información acerca de las especificaciones técnicas del dispositivo Dynex 91000051
- nombre de fabricante y año de fabricación del dispositivo Dynex 91000051
- condiciones de uso, configuración y mantenimiento del dispositivo Dynex 91000051
- marcas de seguridad y certificados que confirmen su concordancia con determinadas normativas

¿Por qué no leemos los manuales de instrucciones?

Normalmente es por la falta de tiempo y seguridad acerca de las funcionalidades determinadas de los dispositivos comprados. Desafortunadamente la conexión y el encendido de Dynex 91000051 no es suficiente. El manual de instrucciones siempre contiene una serie de indicaciones acerca de determinadas funcionalidades, normas de seguridad, consejos de mantenimiento (incluso qué productos usar), fallos eventuales de Dynex 91000051 y maneras de solucionar los problemas que puedan ocurrir durante su uso. Al final, en un manual se pueden encontrar los detalles de servicio técnico Dynex en caso de que las soluciones propuestas no hayan funcionado. Actualmente gozan de éxito manuales de instrucciones en forma de animaciones interesantes o vídeo manuales que llegan al usuario mucho mejor que en forma de un folleto. Este tipo de manual ayuda a que el usuario vea el vídeo entero sin saltarse las especificaciones y las descripciones técnicas complicadas de Dynex 91000051, como se suele hacer teniendo una versión en papel.

¿Por qué vale la pena leer los manuales de instrucciones?

Sobre todo es en ellos donde encontraremos las respuestas acerca de la construcción, las posibilidades del dispositivo Dynex 91000051, el uso de determinados accesorios y una serie de informaciones que permiten aprovechar completamente sus funciones y comodidades.

Tras una compra exitosa de un equipo o un dispositivo, vale la pena dedicar un momento para familiarizarse con cada parte del manual Dynex 91000051. Actualmente se preparan y traducen con dedicación, para que no solo sean comprensibles para los usuarios, sino que también cumplan su función básica de información y ayuda.

Índice de manuales de instrucciones

  • Página 1

    Opsys MW ™ Microplate Washer User’s Guide IMPORTANT Please read carefully before using the Opsys MW[...]

  • Página 2

    Part No. 91000051, Revision B This manual is published by DYNEX Technologies, Inc. Questions or comments regarding the content of this manual can be directed to the address below or to your DYNEX Technologies representative. DYNEX Technologies 14340 Sullyfield Circle Chantilly, VA 20151-1683 USA Tel. (703) 631-7800 (800) 288-2354 Fax. (703) 803-144[...]

  • Página 3

    Warranty and Special Provisions Limited Warranty DYNEX Technologies products are fully guaranteed for one year against defects in parts, materials, and workmanship. Defe ctive parts and materials will be replaced or, at the discretion of DYNEX Technologi es, repaired at no charge for a period of one year and labor required for such repl acement or [...]

  • Página 4

    [...]

  • Página 5

    Table of Contents T able of Content s About this Manual iii Chapter 1 Introduction 1 Description 1 Programming 2 Wash Head 3 Wash Head Positions 4 Wash Fluid 5 Waste 5 Features 7 Specifications 9 Warning Labels 11 Chapter 2 Installation 13 Unpacking 13 Materials Provided 13 Hardware Components 15 Positioning the Instrument 18 Installing the Wash He[...]

  • Página 6

    Table of Contents Cleaning and Decontamination 69 Cleaning the Containers 70 Replacing the Tubing 71 Pump Calibration 74 Chapter 7 Troubleshooting 77 Operational Problems 77 Chapter 8 Service 81 Service Procedures 81 Spare Parts 82 Returning a Washer for Service 83 Contact DYNEX Technologies 85 Index 86 ii Opsys MW™ User’s Guide[...]

  • Página 7

    About this Manual About this Manual This manual has been written for laboratory technicians and provides detailed instructions for using the Opsys MW™ system. This manual gives you the information needed to: • Review safety precautions. • Install the Opsys MW™ system. • Understand the Opsys MW™ menus. • Set the Opsys MW™ system up t[...]

  • Página 8

    About this Manual This page is intentionally left blank iv Opsys MW™ User’s Guide[...]

  • Página 9

    Description Chapter 1 Introduction The Opsys MW™ is a microprocessor-controlled microplate washing system that performs wash protocols that are defined by the user. It is designed to wash all of the wells in one column or one row of a 96-well plate at once. The washing protocol can be programmed so that all of the columns (or rows) are washed in [...]

  • Página 10

    Description Programming All wash protocols on the Opsys MW™ Microplate Washer are user-defined. Up to 40 wash protocols can be contained on the system.  Note: Memory limitations m ay restrict the size and complexity of an individual wash protocol. In addition, the system can be configured with up to ten different plate types so that the wash h[...]

  • Página 11

    Description Wash Head The wash head on the Opsys MW™ Microplate Washer contains multiple sets of dual wash pins . Each set of dual wash pins consists of a dispense pin and an aspirate pin that are closely spaced so that fluid can be aspirated from and dispensed into a well simultaneously. The following wash heads can be fitted on the Opsys MW™:[...]

  • Página 12

    Description Wash Head Positions The vertical positions that the wash head can assume are described below. Each wash head position can be specified by the user to within 0.1 mm.  Note : Procedures for specifying wash head positions for various plate types are described on page 30. Wash Head Position Description Dispense Height Positions the aspir[...]

  • Página 13

    Description Wash Fluid A single wash fluid is used on the Opsys MW™ Microplate Washer. The wash fluid is contained in the Wash Container and is dispensed into the wells whenever a Dispense or Fill operation is specified in the wash protocol. In addition, wash fluid is dispensed into the purge tray during a Purge operation. Dispensing of the fluid[...]

  • Página 14

    Description This page is intentionally left blank 6 Opsys MW™ User’s Guide[...]

  • Página 15

    Features Features The Opsys MW™ system has a number of performance and convenience features. These are summarised below: • Small footprint • Up to 40 wash protocols can be programmed • Up to ten plate types can be programmed • Two wash head configurations (1 x 8 and 1 x 12) • On-board self diagnostics • SHOW function allows visual ver[...]

  • Página 16

    Features This page is intentionally left blank 8 Opsys MW™ User’s Guide[...]

  • Página 17

    Specifications Specifications Dimensions and Weight Length 49 cm (19.3 in) Width 38 cm (15.0 in) Height 19 cm (7.5 in) Weight 9.1 kg (20 lbs) Operation Dispense Range 50 – 999 µ L Dispense Precision 5% CV (with 300 µ L distilled w ater) Residual Aspirate Volumes < 1 µ L for U and V bottom plates < 5 µ L for flat bottom plates Washing Ti[...]

  • Página 18

    Specifications Input Ranges Assay Programming Assay Name Up to 10 characters Password Up to 8 characters Move: Start Strip 1 - 8 for 1 x 8 heads and 1 - 12 for 1 x 12 heads (Increment: 1) Move: End Strip 1 - 8 for 1 x 8 heads and 1 - 12 for 1 x 12 heads (Increment: 1) Move: #Cycles 1 - 9 (Increment: 1) Aspirate: Cycles 1 - 9 (Increment: 1) Dispense[...]

  • Página 19

    Warning Labels Warning Labels The Opsys MW™ or its components may contain certain labels that that either warn the user of a hazard or note an electrical connection. The labels that may be used on the Opsys MW™ system are described below. Label Description Alternating current is present. ( English ) Caution symbol. Refer to the Routine Maintena[...]

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    Warning Labels This page is intentionally left blank 12 Opsys MW™ User’s Guide[...]

  • Página 21

    Unpacking Chapter 2 Inst allation Unpacking Materials Provided The main carton contains the instrument. The contents of this carton are listed below: Article Quantity Opsys MW™ System 1 Instrument Power Cable 1 Cleaning Wire, Aspirate 1 Cleaning Wire, Dispense 1 Instrument Configuration Report 1 User’s Guide 1 Declaration of Conformity 1 Wash H[...]

  • Página 22

    Unpacking To unpack the components: 1. Place the Opsys MW™ instrument in the position where it will be located for use. 2. Place the Wash Container, Waste Container and Foam/Aerosol Trap at the rear of the instrument. 3. Examine the packaging to be sure that the power cord and other materials have been removed. Please save packaging material for [...]

  • Página 23

    Hardware Components Opsys MW™ User’s Guide 15 Hardware Components Hardware components of the Opsys MW™ instrument are shown in Figure 2 and described below.  Note: The Wash Container, Waste Container and Foam/Aerosol Trap are external to the instrument and are not shown in Figure 2. Wash Head Microtiter ® Plate Plate Carrier Display Panel[...]

  • Página 24

    Hardware Components • Keypad . The keypad is used by the operator to select commands and enter wash parameters. • RS232 Interface . The system includes an RS232 interface so that an external computer can be connected for technical service purposes. • Wash Container . (Shown in Figure 1). The wash container contains the wash fluid that is used[...]

  • Página 25

    Hardware Components Connectors at the rear of the Opsys MW™ system are shown in Figure 3. Connections for the power cord, dispense pump power, waste bottle level sensor, waste bottle vacuum line and an external computer are made at the rear of the system.  Important : Be sure that the vacuum exhaust is not obstructed. Figure 3. Rear View of th[...]

  • Página 26

    Positioning the Instrument Positioning the Instrument Determine the area where the system will be located. You will need a firm and level area that is approximately 51 cm (20 inches) wide, 62 cm (24 inches) deep, and 40 cm (16 inches) high for the Opsys MW™. 18 Opsys MW™ User’s Guide[...]

  • Página 27

    Installing the Wash Head Installing the Wash Head The Wash Head must be located on the Wash Head Arm. To install the Wash Head: 1. Position the Arm over the Purge Reservoir portion of the Plate Holder. 2. Place the Wash Head on the two Arm Pins, being careful to properly seat the Wash Head on the support pins. 3. Level the Wash Head in relation to [...]

  • Página 28

    Installing the Wash Container Installing the Wash Container The dispense tubing and dispense pump power cable must be connected. To install the Wash Container: 1. Fill the Wash Container with the wash solution that is to be used.  Note : The Wash Container contains up to 4 liters. 2. Insert the Pick-Up Filter into the Wash Container and secure t[...]

  • Página 29

    Installing the Waste Container Installing the Waste Container To install the Waste Container and Foam/Aerosol Trap: 1. Insert the Quick-Connect end of the vacuum tubing on the Foam/Aerosol Trap to the Quick-Connect vacuum connector at the rear of the instrument Figure 3). 2. Screw on the Waste Container cap. 3. Route the waste tubing through the lo[...]

  • Página 30

    Turning on the System Turning on the System  Note : Depending upon local electrical codes, an uninterruptible power system (UPS) may be required in your laboratory. A UPS is not provided with the Opsys MW™ system. 1. Connect the Opsys MW™ to the laboratory electrical supply outlet.  CAUTION: The Opsys MW™ instrument m ust be connected t[...]

  • Página 31

    The Keypad Opsys MW™ User’s Guide 23 Chapter 3 Configuration The Keypad The keypad is used to access the menus and commands for setting up the Opsys MW™ entering new wash protocols and running the wash protocols. The keypad contains scroll keys , function keys , and alphanumeric key s . The keys on the keypad are shown in Figure 4. 1 ABC DEF [...]

  • Página 32

    The Keypad The use of each of the keys is summarized below: Key Purpose Scroll keys The functionality of the scroll keys depends upon what is being displayed. During display of the main menu (page 26), pressing the up or down scroll keys will display successive wash protocol numbers and names (or dashes, if a protocol is not yet named). Horizontal [...]

  • Página 33

    The Keypad Key Purpose Alphanumeric Keys The numeral shown on the key is displayed by pressing the key once. If the key is pressed two to seven times rapidly, one of the letters on the key will be displayed. The letter that is displayed depends upon the number of times the key is pressed. Opsys MW™ User’s Guide 25[...]

  • Página 34

    Menus Menus The Opsys MW™ menus are hierarchical and are organised by function. There are two main menus: Setup and Program. Menu Purpose SETUP Configure the Instrument PROGRAM Create or edit plate washing protocols * * A protocol includes general information (e.g. the type of microplate to be washed) as well as a listing of the various steps to [...]

  • Página 35

    Menus The SETUP Menu Press the function key under SETUP to display the SETUP menu (Figure 5). The SETUP menu contains commands to configure the instrument. 1: TESTNAME SETUP PROGRAM Select Option VERSION CAL. Select Option HEAD PLATE Figure 5. SETUP Menu The PROGRAM Menu Press the function key under PROGRAM to display the PROGRAM menu (Figure 6). T[...]

  • Página 36

    Configuring the System Configuring the System The Opsys MW™ must be configured before it is operated for the first time. The commands for doing this are contained in the SETUP menu (Figure 7). Use ARROW keys to scroll through the menus. 1: TESTNAME SETUP PROGRAM Select Option VERSION CAL. Select Option HEAD PLATE Figure 7. SETUP Menu The purpose [...]

  • Página 37

    Configuring the System Specifying the Wash Head The HEAD command allows the installed wash head to be specified: 1. Select SETUP. The display will read: Select option HEAD PLATE  2. Select HEAD. The current wash head selection is displayed in the upper line: Wash head? 8 8  If needed, scroll right to view the remaining selection:  Wash hea[...]

  • Página 38

    Configuring the System Specifying Plate Parameters The PLATE command allows plate parameters to be specified for up to 10 plate types. When setting plate parameters, a default value is displayed for each of the wash head positions. The default values are factory-set and represent approximate starting values for each wash head position.  Note : T[...]

  • Página 39

    Configuring the System To specify parameters for a plate ty pe: 1. Obtain an empty plate of the plate type being specified and place it in the plate holder. 2. Select SETUP (if needed). The display will read: Select option HEAD PLATE  3. Select PLATE. The name of the first plate is displayed in the lower line: Select plate 1: - - - - - - - - - -[...]

  • Página 40

    Configuring the System 7. Enter the dispense height using the keypad and press ENTER. The display will read: Well top? 160 SHOW  Note : The default value is displayed. Select SHOW to move the wash head to the default well top position so that the height can be checked. Then, determine the correct value as outlined on page 30. Repeat the procedur[...]

  • Página 41

    Configuring the System Remember if your protocol requires sweeping, that must first be specified in the plate definition used in the protocol. 11. Select YES to specify a sweep on all aspiration cycles. The display will read: Sweep height? 370 SHOW  Note : The default value is displayed. Select SHOW to move the wash head to the default sweep pos[...]

  • Página 42

    Configuring the System 15. Enter the desired value using the keypad and press ENTER. The display will read: Select option HEAD PLATE   Note : If NO is selected for Bottom wash? (Step 13), the display will read as shown above. 34 Opsys MW™ User’s Guide[...]

  • Página 43

    Configuring the System Displaying the Software Version The VERSION command displays the software version that is currently installed on the Opsys MW™ instrument: 1. Select SETUP (if needed). The display will read: Select option HEAD PLATE  2. Scroll right to display the next selection: Select option  VERSION CAL.  3. Select VERSION. The [...]

  • Página 44

    Configuring the System Specifying the Pump Parameters The CAL. command allows the calibration values for each pump to be specified: 1. Select SETUP (if needed). The display will read: Select option HEAD PLATE  2. Scroll right to display the next selection: Select option  VERSION CAL.  3. Select the pump pressure (high or low). The display [...]

  • Página 45

    Configuring the System Selecting Clean The CLEAN command enables the washer to be cleaned automatically at a specified interval. 1. Scroll right to display the next section: Select option  CLEAN 2. Select CLEAN. The display will read: Auto Clean? YES NO 3. Select YES if you want periodic automatic cleaning. The display will read: Interval (Hrs) [...]

  • Página 46

    Configuring the System This page is intentionally left blank 38 Opsys MW™ User’s Guide[...]

  • Página 47

    Wash Protocol Operations Chapter 4 W ash Protocols Wash Protocol Operations A wash protocol consists of a series of Purge , Move , Soak , Aspirate , Dispense and Fill operations. Purge , Move and Soak can be carried out in any sequence. Aspirate , Dispense and Fill can only be carried out within a Move operation, and there cannot be a Move within a[...]

  • Página 48

    Wash Protocol Operations Operation Description Dispense Dispenses a specified amount of fluid into the wells after aspirating the contents of the wells (i.e. wherever a DISPENSE step is added to a protocol, the OPSYS MW will automatically aspirate the wells before dispensing more fluid in order to prevent a well overflow). If a bottom wash is speci[...]

  • Página 49

    Creating a New Wash Protocol Creating a New Wash Protocol Specifying the Header  NOTE: Pressing CANCEL while creating or editing a protocol will bring you back to the main menu without saving your changes.  or  (or use keypad) Select one of the 40 protocol numbers. ↓ PROGRAM Select PROGRAM from the main menu. ↓ (keypad) Enter a protoco[...]

  • Página 50

    Creating a New Wash Protocol To create a new w ash protocol header: 1. Display the main menu. 1: - - - - - - - - - - SETUP PROGRAM 2. Use the up or down scroll keys to display the protocol number (or use the keypad to enter the protocol number) that is to be created: 6: - - - - - - - - - - SETUP PROGRAM  Note : Dashes will be displayed if a prot[...]

  • Página 51

    Creating a New Wash Protocol 7. Re-enter the password and press ENTER. The display will read: Select plate 2: DYNEX_1  Note : The plate name that is shown is an example. The actual name will be the plate name that is entered for that plate type during system configuration (see page 30). 8. Use the up and down scroll keys to select the plate type[...]

  • Página 52

    Creating a New Wash Protocol 10. Select YES if the total elapsed time for washing any number of strips within a plate is to be constant. The display will read: Select option MOVE PURGE  The sequential steps in the wash protocol can now be specified, as outlined on page 45. 44 Opsys MW™ User’s Guide[...]

  • Página 53

    Creating a New Wash Protocol Specifying Wash Cy cle Operations The final step in creating a wash protocol is to specify individual operations within the wash protocol. An example of a wash protocol is shown in 45. In this example, the dispense wash pin s are purged, and then a Dispense and Aspirate are carried out twice on each of the 12 strips (co[...]

  • Página 54

    Creating a New Wash Protocol To specify a Purge:  Note : A purge must be performed outside of a Move cycle. 1. Display the header information for the selected wash protocol, as outlined on page 41. The display will read: Select option MOVE PURGE  2. Select PURGE. The display will read: Fluid volume? 350 3. Use the keypad to enter the volume o[...]

  • Página 55

    Creating a New Wash Protocol To specify a Move cy cle: 1. Display the header information for the selected wash protocol, as outlined on page 41. The display will read: Select option MOVE PURGE  2. Select MOVE. The display will read: Start strip? 1 3. Using the keypad, enter the strip number where the move cycle should start. 4. Press ENTER. The [...]

  • Página 56

    Creating a New Wash Protocol To specify an Aspiration (within a Move):  Note : An Aspirate or Dispense must be performed within a Move cycle. 1. Select ASPIRATE. The display will read: Cycles? 1 2. Using the keypad, enter the number of aspiration cycles that are to be to be performed within the MOVE cycle. 3. Press ENTER. The display will read: [...]

  • Página 57

    Creating a New Wash Protocol To specify a Dispense (within a Move):  Note : A Dispense or Aspirate must be performed within a Move cycle. 1. Select DISPENSE. The display will read: Fluid volume? 350 2. Use the keypad to enter the volume of fluid to be dispensed.  Note : A volume ranging from 50 to 999 microliters can be entered. 3. Press ENTE[...]

  • Página 58

    Creating a New Wash Protocol 6. Press ENTER. The display will read: Cycles? 1 7. Use the keypad to enter the number of bottom wash cycles to be performed and press ENTER. The display will read: Select option ASPIRATE DISPENSE  50 Opsys MW™ User’s Guide[...]

  • Página 59

    Creating a New Wash Protocol To specify a Soak (within a Move):  Note : A Soak can be performed either within or outside of a Move cycle. The procedure below describes the procedure for specifying a soak within a Move cycle. The procedure for specifying a Soak outside of a Move cycle is described on page 50. 1. Scroll right to display the next s[...]

  • Página 60

    Creating a New Wash Protocol To specify a Fill (within a Move):  Note : A Fill must be performed within a M ove cycle. 1. Scroll right to display the next selection. The display will read: Select option  SOAK FILL  2. Select FILL. The display will read: Fluid volume? 50 3. Use the keypad to enter the volume of fluid to be dispensed during [...]

  • Página 61

    Creating a New Wash Protocol Ending a MOVE Cycle 1. Scroll right to display the next selection. The display will read: Select option  SOAK FILL  2. Scroll right to display the next selection. The display will read: Select option  END LOOP 3. Select END LOOP. The display will read: Select option MOVE PURGE  Opsys MW™ User’s Guide 53[...]

  • Página 62

    Creating a New Wash Protocol To specify a Soak (outside of a Move):  Note : A Soak can be performed either within or outside of a Move cycle. The procedure below describes the procedure for specifying a soak outside of a Move cycle. The procedure for specifying a Soak within a Move cycle is described on page 47. 1. Scroll right to display the ne[...]

  • Página 63

    Creating a New Wash Protocol To specify the End of the wash protocol: 1. Scroll right to display the next selection. The display will read: Select option  SOAK END 2. Select END. The display will read: 1: NAME_01 SETUP PROGRAM Opsys MW™ User’s Guide 55[...]

  • Página 64

    Modifying a Wash Protocol Modifying a Wash Protocol An existing wash protocol can be modified by using many of the same commands that are used to create a wash protocol. To modify a wash protocol: 1. Display the main menu: 1: NAME_01 SETUP PROGRAM 2. Use the up and down scroll keys to select the wash protocol to be modified (or enter the number usi[...]

  • Página 65

    Modifying a Wash Protocol 6. The cursor is positioned under the first character of the name. If the name is to be changed, enter the name using the keypad and press ENTER. The first step of the wash protocol will be displayed: MOVE 1-12 1 Cycles DELETE EDIT   Note : The example above shows the MOVE operation as the first step in the protocol.[...]

  • Página 66

    Modifying a Wash Protocol To modify a wash operation: 1. Select EDIT while the operation is displayed. The first step in the operation is displayed: Start strip? 1 Cycles  Note : The actual step that is displayed depends upon the operation being modified. 2. Modify the operation, using the same procedures as when creating a new wash protocol. MO[...]

  • Página 67

    Modifying a Wash Protocol To delete a wash operation: 1. Select DELETE while the operation is displayed. The operation is removed from the wash protocol.  Note : If a MOVE operation is deleted, all operations within the MOVE (i.e., Aspirate , Dispense , Soak and Fill ) are also removed. Opsys MW™ User’s Guide 59[...]

  • Página 68

    Modifying a Wash Protocol To insert a wash operation: 1. Scroll right while the operation that the new operation is to precede is displayed: MOVE 1-10 1 Cycles  INSERT  Note : The inserted operation will take place immediately before the operation that is displayed. 2. Select INSERT. The display will read: Select option MOVE PURGE  3. Sele[...]

  • Página 69

    Copying a Wash Protocol Copying a Wash Protocol An existing wash protocol can be copied to another program number. If a wash protocol is already in that location, it will be overwritten. To copy a wash protocol: 1. Display the main menu: 1: NAME_01 SETUP PROGRAM 2. Use the up and down scroll keys to select the wash protocol to be modified (or enter[...]

  • Página 70

    Copying a Wash Protocol 7. Using the keypad, enter the number of the wash protocol that the commands are to be copied to. If a wash protocol already exists at that location the display will read: Overwrite test? NO YES NO 8. Select YES. The display will read: 7: NAME_04 SETUP PROGRAM 62 Opsys MW™ User’s Guide[...]

  • Página 71

    Preparation Chapter 5 W ashing a Plate Once the parameters for at least one plate type and one wash protocol have been set, the system can be used to wash a plate. Preparation The only preparation that is required is to check the fluid levels in the Wash Container and the Waste Container. Service the containers if necessary. To empty the waste cont[...]

  • Página 72

    Preparation To refill the wash container: 1. Separate the dispense line quick-connector in the cap of the Wash Container. 2. Unscrew the cap of the Wash Container and lay it and the Pick- Up Filter on a clean paper towel. 3. Fill the Wash Container with the wash fluid that is being used.  Note : If the wash solution is being changed, discard the[...]

  • Página 73

    Washing a Plate Washing a Plate A plate is washed by positioning it on the carrier block, selecting the wash protocol to be run, and pressing the START key. To wash a plate: 1. (If needed) Turn on the instrument. The display will read: 1: TESTNAME SETUP PROGRAM 2. Place the microplate on the plate carrier.  Note : Be sure that the plate position[...]

  • Página 74

    Washing a Plate This page is intentionally left blank 66 Opsys MW™ User’s Guide[...]

  • Página 75

    Routine Maintenance Procedures Chapter 6 Routine Maintenance Routine Maintenance Procedures The Opsys MW™ Microplate Washer is constructed from materials that resist chemical attack. If a spill occurs, it should be cleaned up as soon as possible using a cloth moistened with a mild laboratory detergent (If needed, dilute the laboratory detergent a[...]

  • Página 76

    Routine Maintenance Procedures • Replace the dispense (Wash) tubing. • Replace the aspirate (Waste) tubing.  Note : The dispense tubing and aspiration tubing may need to be replaced more frequently than every six months, depending upon the frequency of use and the severity of operating conditions. Contact DYNEX Technologies for information o[...]

  • Página 77

    Cleaning and Decontamination Cleaning and Decontamination The Opsys MW™ is constructed from materials that resist chemical attack. Spills should be cleaned as soon as possible. If you need to decontaminate the Opsys MW™ instrument (for example, before servicing the instrument), clean the system and then decontaminate it as described below.  [...]

  • Página 78

    Cleaning the Containers Cleaning the Containers Procedures for emptying, cleaning and refilling the Wash Container and the Waste Container are described below. To empty and clean the waste container: 1. Unscrew the cap of the Waste Container. Lay the cap and level sensor assembly on a paper towel. 2. Discard the waste in accordance with local regul[...]

  • Página 79

    Replacing the Tubing Replacing the Tubing To replace the dispense tubing: 1. Separate the dispense line quick-connector in the cap of the Wash Container. Unscrew the cap, lay it and the Pick-Up Filter on a clean paper towel. 2. Discard the contents of the Wash Container. Thoroughly rinse the container with deionized water and let it dry. 3. Disconn[...]

  • Página 80

    Replacing the Tubing To replace the vacuum tubing:  Note : This procedure describes replacement of the vacuum tubing connecting the Waste Container to the Foam/Aerosol Trap. The vacuum tubing connecting the Foam/Aerosol Trap to the rear of the instrument should not require changing. 1. Unscrew the Cap of the Waste Container, lay it and the level[...]

  • Página 81

    Replacing the Tubing To replace the waste tubing: 1. Unscrew the Cap of the Waste Container and lay it with the level sensor assembly on a clean paper towel. 2. Discard the contents of the Waste Container in accordance with local regulations. Thoroughly rinse the container with deionized water and let it dry. 3. Disconnect the waste tubing from the[...]

  • Página 82

    Pump Calibration Pump Calibration Each Opsys MW™ washer manifold, or head, (e.g., 1 x 8, 1 x 12) has three calibration values stored in memory. These values are calculated and stored during manufacture at DYNEX Technologies. When the Opsys MW configuration is changed from one manifold to another, the corresponding calibration settings will be rec[...]

  • Página 83

    Pump Calibration 8. If the dispensed volume is too high, decrease the pump speed using the SETUP/CAL function (refer to page 36). • A pump speed change of -10 units roughly corresponds to a well volume change of -10µl. 9. If the dispensed volume is too low, increase the pump speed using the SETUP/CAL function (refer to page 36). • A pump speed[...]

  • Página 84

    Pump Calibration This page is intentionally left blank 76 Opsys MW™ User’s Guide[...]

  • Página 85

    Operational Problems Chapter 7 T roubleshooting Operational Problems Problems that may occur during normal operation of the Opsys MW™ are described below. For each problem, the symptom, probable cause and the means for resolving the problem are shown. Symptom Probable Cause Resolution The instrument fails to power up. The cord is not connected. C[...]

  • Página 86

    Operational Problems Symptom Probable Cause Resolution Liquid is not dispensed from the dispense pins (continued). The wash protocol is incorrectly defined. Check the system configuration and wash protocol for the number fluids and fluid volume. The dispense tubing or the dispense pump power supply is not connected. Verify that the dispense tubing [...]

  • Página 87

    Operational Problems Symptom Probable Cause Resolution Dispense volumes are incorrect. The dispense pins are leaking. Check the pins, seals and tubing for leaks. The dispense pressure is low. Check the seals and tubing for leaks. Check for air leaks. Reseat the tubing in the dispense valve. The dispense pins are clogged. Remove the wash head assemb[...]

  • Página 88

    Operational Problems Symptom Probable Cause Resolution Aspiration is not complete. (continued) Hydrophobic filter may be obstructed. Replace filter. High Waste Level is displayed. The Waste Container is full. Empty the Waste Container (see page 70). The waste level sensor cable is not plugged in. Connect the waste level sensor cable (see page 17). [...]

  • Página 89

    Service Procedures Chapter 8 Service Service Procedures The only user service procedures that can be carried out for the Opsys MW™ are listed below: • Removal and replacement of the wash head assembly • Cleaning of the wash head assembly To remove the wash head assembly: 1. Loosen the two thumbscrews at the front of the Arm/Head assembly. 2. [...]

  • Página 90

    Spare Parts Spare Parts Accessories 13001580 Plate Holder Assembly 13001610 8-Way Head Assembly 13001630 12-Way Head Assembly 352104000 Cleaning Wire, 0.040” 352101800 Cleaning Wire, 0.018” 91000051 User Manual 91000070 OPSYS MW Quick Set Up Instructions 23501800 Bottle Wide Mouth (2120-0010, Nalgene, Wide Mouth Bottle w/Cap) 029006501 Jar, Aer[...]

  • Página 91

    Returning a Washer for Service Returning a Washer for Service If the instrument must be returned for service, it must be cleaned and decontaminated if it has been in contact with potentially infectious body fluids (including human blood), pathological samples, or toxic or radioactive materials.  Note : Refer to page 69 for cleaning and decontami[...]

  • Página 92

    Returning a Washer for Service EQUIPMENT IN TRANSIT IMPORTANT: Please include a copy of this form with each instrument. If your instrument contains a hard drive, please reta in back-up copies of any stored files. Failure to do so may result in the loss of those files . Return Authorization Number: ___________ Contact Technical Service, DYNEX Techno[...]

  • Página 93

    Contact DYNEX Technologies Contact DYNEX Technologies DYNEX Technologies 14340 Sullyfield Circle Chantilly, VA 20151 USA Tel: 703-631-7800 Toll Free: 800-288-2354 Fax: 703-803-1441 www.dynextechnologies.com Opsys MW™ User’s Guide 85[...]

  • Página 94

    Index Index as required maintenance, 68 ASPIRATE, 39 aspiration height , 4 entering, 32 bottom wash specifying, 34 bottom wash height , 4 CAL. command, 36 calibration prototcol , 74 cleaning , 69 Wash Container, 70 Waste Container, 70 configuration, 28 connections, 17 daily maintenance, 67 data bits, 9 decontamination, 69 DISPENSE, 39 dispense heig[...]

  • Página 95

    Index variable strips, 41 warning labels, 11 Wash Container, 5, 16 cleaning, 64 installing, 20 refilling, 64 wash fluid, 5 wash head, 3, 15 cleaning, 81 installing, 19 positions, 4 removal, 81 replacement, 81 types, 3 wash pins, 3 wash pins, 3 wash protocol copying, 61 deleting, 59 inserting, 60 modifying, 56 operations, 39, 45 wash protocols progr[...]