Leica M205 A manual

Leica M125 Leica M165 C Leica M205 C Leica M205 A Manual[...]

Leica M series Manual 2 General Instructions Safety concept Before using your microscope for the first time, please read the "Safety concept" br ochure included with your instrument. It contains addi - tional information about handling and care . Use in clean rooms The Leica M series can be used in clean rooms without any problems . Clea[...]

Leica M series Manual 3 Safety concept The individual modules of the Leica M stereo - microscopy series include an interactive CD-ROM with all r elevant user manuals in 20 other languages. Keep it in a safe place, and readily ac cessible to the user . User manuals and updates are also available for you to download and print from our web site at w w[...]

Leica M series Manual 4 Symbols used W arning of a danger  This symbol indicates especially impor - tant information that must be read and complied with. F ailure to comply can cause the following: Hazards to personnel ★ F unctional disturbances or damaged instru - ★ ments W arning of hazardous electrical voltage This symbol indicates especi[...]

Leica M series Manual 5 Safety regulations Description The individual modules fulfill the highest requirements for observation and documen - tation of Leica stereo microscopes of the M series. Designated use See “Safety concept” booklet ★ Non-intended use See “Safety concept” booklet ★ Never use M series microscopes or their compo - ne[...]

Leica M series Manual 6 Safety regulations (con tinued) Repair , ser vice work See “Safety concept” booklet ★ Only original Leica M icrosystems spare ★ parts may be used. Before opening the instruments, switch off ★ the power and unplug the pow er cable. T ouching the live circuit can cause injury . ★ T ranspor t Use the original packa[...]

Leica M series Manual 7 Safety regulations (con tinued) Direct contact with eyepieces can be a poten tial transmission path for bacterial and viral infec - tions of the eye. The risk can be kept to a minimum by using personal eyepieces for each individual or detachable eyecups. Light sources: saf ety instructions Prot ective measures of the manufac[...]

Leica M series Manual 8 Safety regulations (con tinued) Supply unit Always unplug the supply unit from the power supply: When installing and disassembling the ★ lamp housing Before opening the lamp housing ★ When replacing the high-pressure mercur y ★ lamp and other parts, such as the heat-ab - sorbing filter or the collector during maintena[...]

Leica M series Manual 9 T able of C ontents T ransmitted-light Base TL BFDF: Befor e First U se 3 0 T ransmitted-light Base TL BFDF 3 1 TL RC™ / TL RCI™ 3 2 IsoPro™ Manual Cr oss-stage: Assembly 3 3 IsoPro™ Motoriz ed Cross-stage: Assembly 3 6 Cables: T erminals 4 0 Cables: Cable Duct 4 1 Cables: Diagram 4 2 Leica LED5000 MCI™ 4 3 Leica L[...]

Leica M series Manual 10 Eyepiec e s Magnification F ac tors of the Eyepiec es 5 8 Health Notes 5 9 Dioptric correction 6 0 Dioptric Correction and P arfocality 6 1 Graticules 6 2 Photograph y & V ide o Photograph y & Video 6 4 Photo tubes and C-mounts 6 5 T rinocular video/phototube 50% 6 6 T rinocular video/phototube 100% 6 7 Microscope [...]

Leica M series Manual 11 Acc essorie s Leica PSC C ontroller 9 5 Dimensional Drawing s Leica M125 / M165 C 9 7 Leica M205 C 10 1 Leica LED5000 MCI 10 4 Leica LED5000 RL 10 5 Specications for the Base s T ransmitted-light Base TL ST 10 7 T ransmitted-light Base TL BFDF 10 8 TL RC™ / TL RCI™ 10 9 IsoPro™ Motoriz ed XY Stage 11 0 Appendi x Ca[...]

Leica M series Manual 12 T he L eica M S eries[...]

Leica M series Manual 13 Congr atula tions! Congra tulations on obtaining your new Leica M series stereomicroscope . W e are convinced it will exceed your expectations, as never before have we applied our decades of experience in the areas of optics, mechanical engineering and ergonomics in such an uncompr omising manner . The M series embodies all[...]

Leica M series Manual 14 A Step T owar ds Infinity Ever since their introduction by Horatio S. Greenough, stereomicroscopes have worked according to the optical principles based primarily on Ernst Abbe's research. For over a century, ingenious optics designers and engi - neers hav e w orked t o push magnification, r eso - lution and image ?[...]

Leica M series Manual 15 The Electronics: Comf ort, Con v enience and Safety for y our Experiments Never before have electronics been used as extensively in a Leica series as in the new M series . Optics carrier , tripod, base and illu - minator are all connected using electrical contacts—which provides a number of advan - tages. Reliability for [...]

Leica M series Manual 16 Have a special r equest? Let us know! Leica Microsystems enjo ys an exceptional repu - tation when it comes to devising customer- specific solutions. If you have a special request that cannot be met with standard parts, contact your Leica consultant . We have a solution for every problem. The Modular Design: E verything is[...]

Leica M series Manual 17 Leica engineers were careful to ensure that the new M series—like its predecessors—remains compatible with existing series. This means that objectives, bases, tubes and so on can be reused. Objectives All new objectives of the M series are par focal, meaning that when used with the objec tive nosepiece, they can be repl[...]

Leica M series Manual 18 On W e Go If your new Leica microscope has already been assembled and commissioned by your Leica consultant, click her e to skip through the instal - lation instructions and go directly to the Quick Start Guide on page 46 . If, on the other hand, you are assembling the microscope yourself , continue with the " Assem - [...]

Leica M series Manual 19 Assembly[...]

Leica M series Manual 20 The first step is to c onnect the focusing column of the M series to the corresponding base . T ools used Hex socket screw driver , 3 mm ★ Assembling the column adapter 1. Securely install the column adapter on the column using the four included screw s. Assembling the focusing drive 2. Securely screw the focusing column[...]

Leica M series Manual 21 T ools used Hex socket screw driver , 4 mm ★ Assembling the optics carrier 1. Place the optics carrier on the focusing column so that the screw fit int o the thread provided and the lug fits int o the groov e. 2. Pr ess the optics carrier backwards to the focusing column and scr ew it in place using your other hand. Opt[...]

Leica M series Manual 22 T ube All intermediate tubes that fit between the optics carrier and the binocular tube are fitted in the same manner . T ools used No tools requir ed. ★ Prepara tions 1. Unscrew the positioning screw and r emove the protective co ver . Assembling the tube 2. Push the tube (for example , the inclined binocular tube) int[...]

Leica M series Manual 23 Ey epieces T ools used No tools requir ed. ★ Magnification range Y ou can ex tend the overall mag nification range using available 10×, 16×, 25× and 40× wide - field eyepieces for persons w earing glasses. Prepara tion 1. If you want to use an optional gra ticule, insert it now (page 62). 2. Remove the plastic tube[...]

Leica M series Manual 24 Objective T ools used No tools requir ed. ★ Prepara tion 1. Remove the protective cap on the optics carrier by turning it. Attaching the objective  Hold the objective firmly during assem - bly and disassembly so that it does not fall onto the stage plate. This applies par ticu - larly to the 2× planapochromatic objec[...]

Leica M series Manual 25 Prepara tions  Hold the objectives firmly during assembly and disassembly so that they do not fall onto the stage plate . Move the drive housing all the way upwards ★ and remove the optics carrier , if the carrier has already been installed. Assembly 1. Remove the transport anchor from the objective nosepiece. 2. Rota[...]

Leica M series Manual 26 Objective Nosepiece – A djusting Parfocality The following procedure only has to be carried out once. Afterwards, both objectives are par fo - cal, meaning that the specimen remains in focus when the objective nosepiece is rotated . This procedure must be repeated if you replace either of the two objectives with another .[...]

Leica M series Manual 27 T ools Hex socket screw driver , 2.5 mm ★ Preparing the A X carrier Before the AX carrier is installed, it may first have to be prepared for the optics carrier to be used (Leica M125, M205 C or M165 C). 1. Check the switch position. If the swit ch position corresponds to the ★ optics carrier used, skip the following st[...]

Leica M series Manual 28 T ools Hex socket screw driver , 4 mm ★ Philips screwdriv er ★ Once the AX carrier has prepared for the corre - sponding optics carrier , it can be installed. 1. Move the slide of the AX carrier into mid position. 2. Install the AX carrier on the focusing column. 3. Screw the objective to the A X carrier . 4. Unscrew th[...]

Leica M series Manual 29 T ransmitted-light Base TL ST Unpacking the base The base is deliver ed with the adapter plat e installed. M ake sure the devices are unpacked on a flat , sufficiently dimensioned , and non-slip sur face. Focusing driv e and column 1. Unscrew the ex tension plate from the base using the Allen key provided . 2. Attach your [...]

Leica M series Manual 30 Removing the transport anchors  Before you can use the transmitted- light base for the first time, it is abso - lutely necessar y to remove the two transpor t anchors as follows . T ransmitted-light Base TL BFDF: Befor e First U se Anchor of switching slide Anchor of mirror[...]

Leica M series Manual 31 T ransmitted-light Base TL BFDF Standard delivery The base is deliver ed with the adapter plat e installed. The selec ted stage (IsoPro™ cross- stage or standard stage 10 447 269), and the focusing drive will ha ve to be mount ed later . Stage assembly The TL BFDF transmitted-light base can be equipped with three differ [...]

Leica M series Manual 32 The base is deliver ed with the adapter plat e installed. The selec ted stage (IsoPro™ cross- stage or standard stage 10 447 269), and the focusing drive will ha ve to be mount ed later . Ensure that the instruments are unpacked on a level, adequa tely sized, and nonskid underlay . Stage assembly The transmitt ed-light ba[...]

Leica M series Manual 33 IsoPro™ Manual Cr oss-stage: Assembly Before the IsoPr o™ cross-stage is mounted to the base, the axis containing the control buttons is attached either on the left or the right side of the cross-stage. IsoPro™ Cross-stage Before the IsoPr o™ cross-stage is mounted to the base, the axis containing the control button[...]

Leica M series Manual 34 IsoPro™ Manual Cr oss-stage: Assembly (continued) 1. T ake the glass plate fr om the cr oss-stage and turn it around. 2. Change the gear rod from the left to the right-hand side. Control assembly 1. T ake the glass plate fr om the cr oss-stage and turn it around. 2. Attach the axis with the control buttons to the desired [...]

Leica M series Manual 35 IsoPro™ Manual Cr oss-stage: Assembly (continued) 3. Screw the cross-stage evenly onto the thr ee threaded holes. 4. Push the cross-stage all the way back towar ds the column. 5. Reinsert the glass plate back into the stan - dard stage. Focusing driv e and column 1. Unscrew the ex tension plate from the base using the All[...]

Leica M series Manual 36 Basics The transmitted light bases of the TL series ( TL BFDF , TL RC™, TL RCI™) are supplied with an installed extension plate. The selec ted stage (IsoPro™ cross-stage or standard stage 10 447 269), and the focusing drive will have to be mounted later . The motorized cross-stage is a sensitive preci - sion instrumen[...]

Leica M series Manual 37 3. Remove the two M3 screws and the holder from the cross-stage . 4. Remove the two M4 screws and the sleeve from the cross-stage . 5. Remove the four shock-absorbing cartons from the cross-stage . After removal, keep all the trans - port anchors in the plastic bag for future transport. IsoPro™ Mot orized Cross-stage: Ass[...]

Leica M series Manual 38 IsoPro™ Mot orized Cross-stage: Assembly (c ontinued) The bases The TL BFDF , TL RC™ and RCI™ transmitted light bases can be equipped with three different stages: standard stage, manual and IsoPro™ automated cross-stage. The selected stage is mounted on the base befor e commissioning . Y ou can switch between the s[...]

Leica M series Manual 39 IsoPro™ Mot orized Cross-stage: Assembly (c ontinued) Cross-stage for X - Y Stage Control Module 1. Plug the CTL2 plug of the cross-stage motor into one of the three available C TL2 inter - faces. 2. Plug the CTL2 plug of the Leica PSC contr ol - ler into another CTL2 inter face. The third C TL2 interface is available for[...]

Leica M series Manual 40 The new Leica M-series features extensive encoding with which various microscope data and settings can be read out, transferred to the PC and reproduc ed later . The inter face to the optics carrier is on the ★ column. The T erminals The connection to the PC and to other instru - ments is made using the terminals on the r[...]

Leica M series Manual 41 The integrated cable duct in the column enables a neat cable layout around the microscope. F or example, the USB or FireW ire cables of the camera can be stow ed in the cable duct. Feeding the cables 1. Unscrew the three screws on the cable duct. 2. Remove the c over of the cable duct. 3. Place the cables in the cable duct [...]

Leica M series Manual 42 Cables: Diagram TL RCI™ DCI Controlbo x PSC PC USB (Connect to fr ee CTL2-port on last footswitch.) Connector from Cr oss Stage Smart Move Pow er/USB LED5000 RL LED5000 MCI™ Footswit ch 1 Footswit ch 2... Please use the Leic a Application Suite to configur e the functions of the system. Alternative installa tion 115/23[...]

Leica M series Manual 43 The L eica LED5000 MCI™ (f or "Multi C ontrast Illumination") is installed using t wo screws. For optimum accessibility, the optics carrier should be remov ed during installation (see page 15 .) Constrain ts The Leica LED5000 MCI™ cannot be used together with the objective nosepiece. Assembly  1. Hold the L[...]

Leica M series Manual 44 Under certain cir cumstances, the light source must not be moved along with the optics carrier . A typical example is multifocus images in which the Z-stack changes while the angle of incidence of the light must remain the same. F or such purposes, the Leica LED5000 MCI™ is directly fastened to the column. Installation on[...]

Leica M series Manual 45 The ring illuminator , the Leica LED5000 RL ("Ring Light"), is installed on the objective using a single scr ew . It has been optimized for a work - ing distance between 60 and 70 mm. Constrain ts The Leica LED5000 RL can be used in conjunc - tion with the planapochromat 1× and planapo - chromat 0.63× objectives[...]

Leica M series Manual 46 Q uick Star t Guide[...]

Leica M series Manual 47 The F astest Route to Suc cess Y our Leica stereomicroscope has been delivered in completely assembled condition by your Leica par tner , and naturally you want to get right to work. Ther efore , your next step should be to study the Q uick Star t Guide, which outlines the most important steps at a glance. This manual will [...]

Leica M series Manual 48 Overview of an M series Microscope 1 Magnification changer (zoom) 2 Switch click stops on/off 3 Coarse/fine focusing 4 F astening screw for the binocular tube (or acc essories) 5 Interchangeable objective 6 Adjustable eyepiec e tubes 7 Eyepiec es for spectacle wearers with dioptric correction and eyecups 8 F astening scr[...]

Leica M series Manual 49 The C orrect Interpupillary D istance The interpupillary distance is correctly set if you see a single circular image field when look ing at a specimen. If y ou ar e still a novice microscope user , you may need a shor t time to become accustomed to this. Not to worry—after a little while, it will become automatic . Refe[...]

Leica M series Manual 50 If you wear glasses: 1. Hold the eyepiece firmly and rotate the eyecups counter clockwise t owards the rear , as other wise the viewing distance is too great. 2. If an eyepiece is equipped with the inte - grated dioptric c orrection, turn the value to the "0" mark. By the way , one benefit of viewing with eyegla[...]

Leica M series Manual 51 F ocusing F ocusing raises or lowers the stereomicroscope using the focusing drive. The specimen detail is brought into sharp focus as soon as it is in the focal point of the objective. The focusing drive can be operated either ★ left- or right-handed. Focusing The inner , coarse adjustment is used for ★ covering g reat[...]

Leica M series Manual 52 Adjusting the r esistance of the focus driv e Adjusting the resistance Is the focus movement too loose or too tight? Does the outfit tend to slide downwards? The resistance can be adjusted individually depend - ing on the equipment weight and personal prefer ences as follow s: 1. Grip the outer driv e knobs with both hands[...]

Leica M series Manual 53 Changing the magnification (zoom) All M series microscopes have an integrated zoom. The name indicates the zoom range cover ed: Leica M125 = 16.5:1 ★ Leica M165 C = 16.5:1 ★ Leica M205 C = 20.5:1 ★ The rotar y knob for the z oom can be used either left or right-handed. Zooming 1. Look int o the eyepieces . 2. F ocus [...]

Leica M series Manual 54 The zoom butt on can optionally be operat ed either with or without ratchet steps. Continu - ous z oom is possible when the ratchet steps are disabled, which many users find convenient. On the other hand, when the ratchet steps are enabled, photographs , measurement results etc. can be repr oduced more ac curately . Enabli[...]

Leica M series Manual 55 Parfocality: More Comf or t and Con venience for Y our W ork All Leica stereomicroscopes are parfocally matched, meaning that you can view a focused specimen from the lowest to the highest magni - fication without having to refocus . There is no need to readjust the focus to view of specimen location that is located higher[...]

Leica M series Manual 56 The iris diaphragm in the optics carrier of your M series microscope has the same purpose as those in a camer a shutter: it regulates the avail - able light, which changes the depth of field. The "depth of field" is the area of a specimen that is brought into sharp f ocus. Closing the iris diaphragm Close the ir[...]

Leica M series Manual 57 E y epiec es[...]

Leica M series Manual 58 Magnification F ac tors of the Ey epieces An eyepiece not only makes it possible to look passively into the micro - scope, but also has a critical effect on the maximum magnification. The magnification factor is between 10x and 40x. The following e yepieces ar e available f or the M series: Magnification Dioptric corre[...]

Leica M series Manual 59 Health Notes Pot ential sources of infection  Direct contact with eyepieces is a potential transmission method for bacterial and viral infections of the ey e. The risk can be kept to a minimum by using individual eyepieces or detachable eyecups. Eyecups can be ordered separately . Please contact your Leica partner . Sepa[...]

Leica M series Manual 60 All Leica eyepieces are also available with built-in dioptric correction, allowing the micro - scope to be used without glasses ev en by those with vision problems. The correction comprises ±5 diopter settings. Using the Dioptric Corr ection 1. Set the dioptric correction of both eyepieces to the mid position ("0"[...]

Leica M series Manual 61 Adjusting 1. Set the dioptric correction for both eyepieces to "0" . 2. Select the lowest magnification and focus on a flat specimen. 3. Select the highest magnification and read - just the sharpness. 4. Select the lowest magnification again, but do not look into the eyepiec es. 6. Rotate the eyepieces c ounte[...]

Leica M series Manual 62 Graticules 2. Clamp the graticule on the insert, applying moderate pressure. Ensure that the gr ati - cule fits tightly . 3. Screw the insert and graticule firmly in to place and replace the ey epiece in the tube. 4. Y ou can now align the graticule by rotating the eyepiece in the tube and then tighten - ing it using the [...]

Leica M series Manual 63 Photogr aph y & V ideo[...]

Leica M series Manual 64 F or most microscope users, digital documen - tation has become an invaluable par t of their work. Research results can be presented in an attractive manner ; measurements on the digi - tal image provide clarity and, in conjunction with the motorized IsoPro™ cross-stage, even images of large specimens can be captured step[...]

Leica M series Manual 65 Photo tubes and C-mounts Application All Leica DFC cameras are equipped with a standardized C-mount inter face. In turn, the C-mount adapter for the respective trinocular tube is connected t o this int er face. This adapter creates a solid mechanical c onnection between the microscope and camera and ensures opti - mum rende[...]

Leica M series Manual 66 T rinoc ular video/phototube 50% Use With its third beam path, the trinocular video/ phototube 50% enables you to simultaneously view and photograph a specimen. The available light is divided as follows: 50% is available for the two ey epieces. ★ 50% of the light is diver ted to the video/ ★ photo beam path. Assembly F [...]

Leica M series Manual 67 Use With its third beam path, the trinocular video/ phototube 100% enables you to either view or photograph a specimen. This means that 100% of the light is available to one or the other beam path. The other beam path remains opaque or black. Assembly F asten the " trinocular tube 100%" to the optics carrier inste[...]

Leica M series Manual 68 Microsc ope C arrier[...]

Leica M series Manual 69 Stereoscopic viewing A stereoscopic view is required for examining spatial objects. For this purpose, the micro - scope carrier AX must be brought to its home position. 1. Unscrew the clamping scr ew . 2. Push the microscope into the home posi - tion (mid position) to obtain a spatial view and tighten the clamping screw . T[...]

Leica M series Manual 70 Parallax -free images The parallax-free imaging produces more precise results in photography , measuring and polarization. 1. Unscrew the clamping scr ew . 2. Push the microscope towards the lef t as far as it will go and tighten the clamping screw . The objec tive is now located directly beneath either the left or the righ[...]

Leica M series Manual 71 Use The objec tive nosepiece enables you to switch between two objectives using just one hand movement , f or example bet ween a 1× and a 2× planapochromat. Parfocal work With the new M series objectives, the parfo - cality is maintained even when objec tives are changed, meaning that the specimen remains in sharp focus d[...]

Leica M series Manual 72 Objec tiv es and optic al ac c essories[...]

Leica M series Manual 73 The Differ ent T ypes of Objec tives T o meet the various requirements regarding imaging properties, there is a choice of high- quality interchangeable planachromatic and planapochromatic objectives and also lower- priced inter changeable achromatic objectives. Achroma tic objectives are par ticularly ★ suited for specim[...]

Leica M series Manual 74 Bases[...]

Leica M series Manual 75 T ransmitted-light Base TL ST : Con trols 1 Adapter plate for easy assembly of focusing drives 2 Removable glass plat e 3 Controller for light int ensity 4 Adjustment for deflection mirror Rear side of the transmitted-light base TL ST 1 Screws for changing the halogen lamp 2 Pow er connection socket 3 Pow er switch Extensi[...]

Leica M series Manual 76 T ransmitted-light Base TL ST : Operation Light intensity contr ol The left control adjusts the intensity of the 12 V/20 W halogen illumination. 1. Switch on the illumination of the base at the power switch. 3. F ocus on the specimen. 3. Set the illumination to the desir ed int ensity using the left control. T ransmitted-li[...]

Leica M series Manual 77 Changing the Halogen Lamp  Before you change the lamp , it is abso - lutely nec essary to unplug the pow er plug from the base to prevent the risk of elec - tric shock! The halogen lamp becomes ver y hot during operation. Therefore , to avoid being burned, let the base cool off for approx. 10 minutes after switching it [...]

Leica M series Manual 78 T ransmitted-light Base TL BFDF: C ontrols 1 Adapter plate for easy assembly of focusing drives 2 Standard stage 10 447 269 3 Button to toggle between bright field and dark field 1 2 3 Extension plate of the T ransmitted-light Base TL BFDF Adapter at the focusing drive Button to toggle between bright field/ dark field C[...]

Leica M series Manual 79 Light intensity contr ol  Please observe the user manual—in particular , all safety regulations—fr om the manufacturers of the light guide and cold light source . Switch on the cold ligh t source according to ★ the manufac turer's user manual and adjust the brightness. T ransmitted-light control The TL BFDF tr[...]

Leica M series Manual 80 TL RC™ / TL RCI™: Controls V ertical column - transmitted-light base adapt er plate 1 Heat sink of the integrat ed halogen illumina - tion (only TL RCI™) 2 Extension plate for focusing drives 3 Standard stage 10 447 269 4 Filter holder 5 Control of t op and bottom flaps of the Rottermann Contr ast™ 6 Button for mir[...]

Leica M series Manual 81 TL RCI™: The Deflec tion Mirror Inv erted world? Depending on the properties of the specimen (refractive index of the environment) and the perception of the viewer , it is possible that the switches described below for positive and inverted relief contrast are to be operated in reverse . This means that the lower switch,[...]

Leica M series Manual 82 TL RCI™: Color In tensity and T emperatur e 1 Potentiomet er for controlling the C CIC™ (Constant C olor Intensity Control) illumina - tion intensity 2 Potentiomet er for controlling the IsoC ol™ color temper ature The transmitted-light base TL RCI™ has t wo electronic potentiometers that contr ol the color intensit[...]

Leica M series Manual 83 Setting the relief contrast The two switches on the left side of the trans - mitted-light base TL RC™/TL RCI™ activate two built-in flaps. The outer switch (1) controls the inverted relief contrast, and the inner switch (2) adjusts the positive relief c ontrast. Depending on the flap position, a par t of the opening o[...]

Leica M series Manual 84 TL RCI™: Methods in T ransmitted Light V ertical bright-field illumination Suitable for stained amplitude specimens with sufficient contrast. The light beams are deflected vertically through the specimen. This results in an accurate bright field with maximum brightness. Inclined transmitted light Suitable for semitrans[...]

Leica M series Manual 85 TL RCI™: Relief Images Starting position 1. Push the deflection mirror all the way back towar ds the column. 2. T urn the deflec tion mirr or into the notch position at an angle of 45°. Positiv e relief contr ast Suitable for semitransparent and transparent specimens. The phase structures look like hills. The effect c[...]

Leica M series Manual 86 Dynamic relief contrast Suitable for semitransparent and transparent specimens. By tilting the deflection mirror slightly , you can move the gaps over the entire field of view and quickly toggle bet ween positive and negative relief images. The dynamic effec t makes it easy to distinguish phase struc tures from amplitude[...]

Leica M series Manual 87 Filters f or TL RC™ and TL RCI™ The transmitted-light bases TL RC™ and TL RCI™ can be equipped with up to three filters—available as accessories—simultane - ously . By customer request, the filters are also available as one- off items. 1. Switch off the light sour ce or click ( TL RCI™) the button for the [...]

Leica M series Manual 88 IsoPro™ (Non-mot orized): C ontrols Operating the cross-stage IsoPr o™ 1. T o move the stage in X direction, rotate the outer knob. 2. T o move the stage in Y direction, rotate the inner control ring . 1 2[...]

Leica M series Manual 89 IsoPro™ (Mot orized): C ontrols 1 Motorized cross -stage Leic a IsoPro ™ 2 Housing with motorization 1 Sub-D interface for Leic a SmartMove™ 2 3 CTL2 interfaces 3 USB interface (type B) 4 Socket for grounded pow er cable 1 Quick control/memory function 2 Fine c ontrol in X direction 3 Fine c ontrol in Y direction 1 2 [...]

Leica M series Manual 90 S y st em Illumina tion[...]

Leica M series Manual 91 Use The L eica LED5000 MCI™ (f or "Multi C ontrast Illumination") is a universal high-output illu - minator . Three groups of 3 LEDs each can be arranged as desired around the specimen. The targeted contr ol of the LEDs enables various illumination scenarios to be implement ed. Prepara tion Hold the MCI with bot[...]

Leica M series Manual 92 Using the ke yboard Use the ★ key to switch the illumination on or off . Use the "+" and "–" keys to adjust the ★ brightness in 10 increments . T ouch either of the two butt ons to adjust the intensity in small increments. Hold a key to change the intensity more quickly . The illumination scenario[...]

Leica M series Manual 93 Leica LED5000 RL Use The Leica LED5000 RL ("Ring Light") generates a ver y bright and homogenous incident light. It has a diameter of 80 mm and illuminates the specimen with 48 LEDs that can be switched on and off completely or in various combinations. It is controlled using either the integra ted or via the Leic[...]

Leica M series Manual 94 A cc essories[...]

Leica M series Manual 95 Leica PSC Con troller Use The Leica PSC controller gives you precision control of the Leica IsoPr o™ motorized cross- stage. Connection Connect the Leica PSC controller and the Leica IsoPro™ mot orized cross-stage . Passing ov er the specimen quickly Move the joystick in any desired direction ★ (including diagonally) [...]

Leica M series Manual 96 Dimensional D ra wings[...]

Leica M series Manual 97 Leica M125 / M165 C 120 120 277 24 400 455 292 217 120 120 277 24 400 455 292 217 120 120 277 24 400 455 292 217 Leica M125 / M165 C with incident light base and new focusing c olumn (dimensions in mm)[...]

Leica M series Manual 98 Leica M125 / M165 C (continued) 340 430 85 154 356 154 292 217 106 431 537 340 430 85 154 356 154 292 217 106 431 537 340 430 85 154 356 154 292 217 106 431 537 Leica M125 / M165 C with transmitted-light base TL ST and manual focus (dimensions in mm)[...]

Leica M series Manual 99 Leica M125 / M165 C (continued) 385 154 340 390 440 95 154 116 431 547 292 217 385 154 340 390 440 95 154 116 431 547 292 217 385 154 340 390 440 95 154 116 431 547 292 217 Leica M125 / M165 C with transmitted-light stand TL RC™ and manual focus (dimensions in mm)[...]

Leica M series Manual 100 Leica M125 / M165 C (continued) 497 292 217 187 154 max. 370 X = +-75 mm 370 95 300 249 Y = +-50 mm 116 431 547 497 292 217 187 154 max. 370 X = +-75 mm 370 95 300 249 Y = +-50 mm 116 431 547 497 292 217 187 154 max. 370 X = +-75 mm 370 95 300 249 Y = +-50 mm 116 431 547 Leica M125 / M165 C with transmitted-light stand TL [...]

Leica M series Manual 101 Leica M205 C 370 154 340 390 90 154 292 217 116 431 547 370 154 340 390 90 154 292 217 116 431 547 370 154 340 390 90 154 292 217 116 431 547 Leica M205 C with transmitted-light stand TL ST and manual focusing drive (dimensions in mm)[...]

Leica M series Manual 102 Leica M205 C (continued) 385 154 340 390 440 95 154 116 431 547 292 217 385 154 340 390 440 95 154 116 431 547 292 217 385 154 340 390 440 95 154 116 431 547 292 217 Leica M205 C with transmitted-light stand TL RCI™ and manual focusing drive (dimensions in mm)[...]

Leica M series Manual 103 Leica M205 C (continued) 497 187 154 max. 410 X = +-75 mm 370 95 300 249 Y = +-50 mm 292 217 116 431 547 497 187 154 max. 410 X = +-75 mm 370 95 300 249 Y = +-50 mm 292 217 116 431 547 497 187 154 max. 410 X = +-75 mm 370 95 300 249 Y = +-50 mm 292 217 116 431 547 Dimensions of Leica M205 C with transmitted-light stand TL [...]

Leica M series Manual 104 Leica LED5000 MCI 132 275.3 157 83.5 112 45 112 90 45 36 22 132 275.3 157 83.5 112 45 112 90 45 36 22 132 275.3 157 83.5 112 45 112 90 45 36 22 Leica LED5000 MCI (dimensions in mm)[...]

Leica M series Manual 105 Leica LED5000 RL 120 ø70 30 153 153 ø80[...]

Leica M series Manual 106 Specific a tions f or the Bases[...]

Leica M series Manual 107 T ransmitted-light Base TL ST Light source Halogen lamp 12V/20W Quick illuminant change Ye s Illuminated area 50 mm Po wer supply unit Input voltage 100 – 240 V~, frequency 50 – 60 Hz energy consumption 30 W max., ambient temperatur e 10–40 °C Connections Po wer plug , power switch W eight 7.4 kg Illumination types [...]

Leica M series Manual 108 T ransmitted-light Base TL BFDF Light source Ex ternal via cold light source Illuminated area 40 mm Connections Connection for cold light guide , active f=10 mm, end tube f=13 mm W eight 5.8 kg Illumination types Bright field Ye s Dark field Ye s Oblique light No Relief Contrast S ystem (RC™) No CCIC ( Constant C olor [...]

Leica M series Manual 109 TL RC™ / TL RCI™ Leica TL RC™ Leica TL RCI™ Light source External via cold light source Halogen lamp 12V/20W Quick illuminant change – Ye s Illuminated area 35 mm 35 mm Po wer supply unit – 100 – 240 V~, frequency 50 – 60 Hz energy consumption 30 W max., ambient temperatur e 10–40 °C Connections Connecti[...]

Leica M series Manual 110 IsoPro™ Mot orized XY Stage Compatibility Leica inciden t light base and TL bases (TL BFDF , TL RC™, TL RCI™) Stage size (L × W × H) 335.5 mm × 370 mm × 41.5 mm Tr a v e l 152 mm × 102 mm (6" × 4") Speed X,Y 30 mm/s Motor resolution X,Y 0.25 µm Repeatability X, Y ± 2 µm Positioning ac curacy X,Y ±[...]

Leica M series Manual 111 Appendix[...]

Leica M series Manual 112 Calculating the T otal Magnific ation and F ield of View Diameter Paramet er MO Magnification of objective ME Magnification of eyepiece z Magnification changer position q T ube factor , e.g . 1.5× for coaxial incident light, 1.6× for 45° Ergo T ube™ r F ac tor 1.25x if the planachromatic and planapochromatic objec[...]

Leica M series Manual 113 Care , Maintenance, C ontac t Persons W e hope you enjoy using your high-per for - mance microscope. Leica microscopes are renowned for their r obustness and long ser vice life. Observing the following care and clean - ing tips will ensure that even after years and decades, your Leica microsc ope will continue to work as w[...]

Leica M series Manual 114 Care , Maintenance, C ontac t Persons (c ontinued) Protection from dirt Dust and dir t will affect the qualit y of your results. Put a dust cover over the instrument when ★ it will not be used for a long time. Use dust caps to protect tube openings, ★ tubes without eyepieces , and eyepieces. Keep accessories in a dust[...]