Dynex 91000051 manuel d'utilisation
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Un bon manuel d’utilisation
Les règles imposent au revendeur l'obligation de fournir à l'acheteur, avec des marchandises, le manuel d’utilisation Dynex 91000051. Le manque du manuel d’utilisation ou les informations incorrectes fournies au consommateur sont à la base d'une plainte pour non-conformité du dispositif avec le contrat. Conformément à la loi, l’inclusion du manuel d’utilisation sous une forme autre que le papier est autorisée, ce qui est souvent utilisé récemment, en incluant la forme graphique ou électronique du manuel Dynex 91000051 ou les vidéos d'instruction pour les utilisateurs. La condition est son caractère lisible et compréhensible.
Qu'est ce que le manuel d’utilisation?
Le mot vient du latin "Instructio", à savoir organiser. Ainsi, le manuel d’utilisation Dynex 91000051 décrit les étapes de la procédure. Le but du manuel d’utilisation est d’instruire, de faciliter le démarrage, l'utilisation de l'équipement ou l'exécution des actions spécifiques. Le manuel d’utilisation est une collection d'informations sur l'objet/service, une indice.
Malheureusement, peu d'utilisateurs prennent le temps de lire le manuel d’utilisation, et un bon manuel permet non seulement d’apprendre à connaître un certain nombre de fonctionnalités supplémentaires du dispositif acheté, mais aussi éviter la majorité des défaillances.
Donc, ce qui devrait contenir le manuel parfait?
Tout d'abord, le manuel d’utilisation Dynex 91000051 devrait contenir:
- informations sur les caractéristiques techniques du dispositif Dynex 91000051
- nom du fabricant et année de fabrication Dynex 91000051
- instructions d'utilisation, de réglage et d’entretien de l'équipement Dynex 91000051
- signes de sécurité et attestations confirmant la conformité avec les normes pertinentes
Pourquoi nous ne lisons pas les manuels d’utilisation?
Habituellement, cela est dû au manque de temps et de certitude quant à la fonctionnalité spécifique de l'équipement acheté. Malheureusement, la connexion et le démarrage Dynex 91000051 ne suffisent pas. Le manuel d’utilisation contient un certain nombre de lignes directrices concernant les fonctionnalités spécifiques, la sécurité, les méthodes d'entretien (même les moyens qui doivent être utilisés), les défauts possibles Dynex 91000051 et les moyens de résoudre des problèmes communs lors de l'utilisation. Enfin, le manuel contient les coordonnées du service Dynex en l'absence de l'efficacité des solutions proposées. Actuellement, les manuels d’utilisation sous la forme d'animations intéressantes et de vidéos pédagogiques qui sont meilleurs que la brochure, sont très populaires. Ce type de manuel permet à l'utilisateur de voir toute la vidéo d'instruction sans sauter les spécifications et les descriptions techniques compliquées Dynex 91000051, comme c’est le cas pour la version papier.
Pourquoi lire le manuel d’utilisation?
Tout d'abord, il contient la réponse sur la structure, les possibilités du dispositif Dynex 91000051, l'utilisation de divers accessoires et une gamme d'informations pour profiter pleinement de toutes les fonctionnalités et commodités.
Après un achat réussi de l’équipement/dispositif, prenez un moment pour vous familiariser avec toutes les parties du manuel d'utilisation Dynex 91000051. À l'heure actuelle, ils sont soigneusement préparés et traduits pour qu'ils soient non seulement compréhensibles pour les utilisateurs, mais pour qu’ils remplissent leur fonction de base de l'information et d’aide.
Table des matières du manuel d’utilisation
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Page 1
Opsys MW ™ Microplate Washer User’s Guide IMPORTANT Please read carefully before using the Opsys MW[...]
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Part No. 91000051, Revision B This manual is published by DYNEX Technologies, Inc. Questions or comments regarding the content of this manual can be directed to the address below or to your DYNEX Technologies representative. DYNEX Technologies 14340 Sullyfield Circle Chantilly, VA 20151-1683 USA Tel. (703) 631-7800 (800) 288-2354 Fax. (703) 803-144[...]
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Warranty and Special Provisions Limited Warranty DYNEX Technologies products are fully guaranteed for one year against defects in parts, materials, and workmanship. Defe ctive parts and materials will be replaced or, at the discretion of DYNEX Technologi es, repaired at no charge for a period of one year and labor required for such repl acement or [...]
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Table of Contents T able of Content s About this Manual iii Chapter 1 Introduction 1 Description 1 Programming 2 Wash Head 3 Wash Head Positions 4 Wash Fluid 5 Waste 5 Features 7 Specifications 9 Warning Labels 11 Chapter 2 Installation 13 Unpacking 13 Materials Provided 13 Hardware Components 15 Positioning the Instrument 18 Installing the Wash He[...]
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Table of Contents Cleaning and Decontamination 69 Cleaning the Containers 70 Replacing the Tubing 71 Pump Calibration 74 Chapter 7 Troubleshooting 77 Operational Problems 77 Chapter 8 Service 81 Service Procedures 81 Spare Parts 82 Returning a Washer for Service 83 Contact DYNEX Technologies 85 Index 86 ii Opsys MW™ User’s Guide[...]
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About this Manual About this Manual This manual has been written for laboratory technicians and provides detailed instructions for using the Opsys MW™ system. This manual gives you the information needed to: • Review safety precautions. • Install the Opsys MW™ system. • Understand the Opsys MW™ menus. • Set the Opsys MW™ system up t[...]
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About this Manual This page is intentionally left blank iv Opsys MW™ User’s Guide[...]
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Description Chapter 1 Introduction The Opsys MW™ is a microprocessor-controlled microplate washing system that performs wash protocols that are defined by the user. It is designed to wash all of the wells in one column or one row of a 96-well plate at once. The washing protocol can be programmed so that all of the columns (or rows) are washed in [...]
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Description Programming All wash protocols on the Opsys MW™ Microplate Washer are user-defined. Up to 40 wash protocols can be contained on the system. Note: Memory limitations m ay restrict the size and complexity of an individual wash protocol. In addition, the system can be configured with up to ten different plate types so that the wash h[...]
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Description Wash Head The wash head on the Opsys MW™ Microplate Washer contains multiple sets of dual wash pins . Each set of dual wash pins consists of a dispense pin and an aspirate pin that are closely spaced so that fluid can be aspirated from and dispensed into a well simultaneously. The following wash heads can be fitted on the Opsys MW™:[...]
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Description Wash Head Positions The vertical positions that the wash head can assume are described below. Each wash head position can be specified by the user to within 0.1 mm. Note : Procedures for specifying wash head positions for various plate types are described on page 30. Wash Head Position Description Dispense Height Positions the aspir[...]
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Page 13
Description Wash Fluid A single wash fluid is used on the Opsys MW™ Microplate Washer. The wash fluid is contained in the Wash Container and is dispensed into the wells whenever a Dispense or Fill operation is specified in the wash protocol. In addition, wash fluid is dispensed into the purge tray during a Purge operation. Dispensing of the fluid[...]
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Description This page is intentionally left blank 6 Opsys MW™ User’s Guide[...]
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Features Features The Opsys MW™ system has a number of performance and convenience features. These are summarised below: • Small footprint • Up to 40 wash protocols can be programmed • Up to ten plate types can be programmed • Two wash head configurations (1 x 8 and 1 x 12) • On-board self diagnostics • SHOW function allows visual ver[...]
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Features This page is intentionally left blank 8 Opsys MW™ User’s Guide[...]
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Specifications Specifications Dimensions and Weight Length 49 cm (19.3 in) Width 38 cm (15.0 in) Height 19 cm (7.5 in) Weight 9.1 kg (20 lbs) Operation Dispense Range 50 – 999 µ L Dispense Precision 5% CV (with 300 µ L distilled w ater) Residual Aspirate Volumes < 1 µ L for U and V bottom plates < 5 µ L for flat bottom plates Washing Ti[...]
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Specifications Input Ranges Assay Programming Assay Name Up to 10 characters Password Up to 8 characters Move: Start Strip 1 - 8 for 1 x 8 heads and 1 - 12 for 1 x 12 heads (Increment: 1) Move: End Strip 1 - 8 for 1 x 8 heads and 1 - 12 for 1 x 12 heads (Increment: 1) Move: #Cycles 1 - 9 (Increment: 1) Aspirate: Cycles 1 - 9 (Increment: 1) Dispense[...]
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Page 19
Warning Labels Warning Labels The Opsys MW™ or its components may contain certain labels that that either warn the user of a hazard or note an electrical connection. The labels that may be used on the Opsys MW™ system are described below. Label Description Alternating current is present. ( English ) Caution symbol. Refer to the Routine Maintena[...]
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Warning Labels This page is intentionally left blank 12 Opsys MW™ User’s Guide[...]
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Unpacking Chapter 2 Inst allation Unpacking Materials Provided The main carton contains the instrument. The contents of this carton are listed below: Article Quantity Opsys MW™ System 1 Instrument Power Cable 1 Cleaning Wire, Aspirate 1 Cleaning Wire, Dispense 1 Instrument Configuration Report 1 User’s Guide 1 Declaration of Conformity 1 Wash H[...]
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Page 22
Unpacking To unpack the components: 1. Place the Opsys MW™ instrument in the position where it will be located for use. 2. Place the Wash Container, Waste Container and Foam/Aerosol Trap at the rear of the instrument. 3. Examine the packaging to be sure that the power cord and other materials have been removed. Please save packaging material for [...]
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Hardware Components Opsys MW™ User’s Guide 15 Hardware Components Hardware components of the Opsys MW™ instrument are shown in Figure 2 and described below. Note: The Wash Container, Waste Container and Foam/Aerosol Trap are external to the instrument and are not shown in Figure 2. Wash Head Microtiter ® Plate Plate Carrier Display Panel[...]
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Page 24
Hardware Components • Keypad . The keypad is used by the operator to select commands and enter wash parameters. • RS232 Interface . The system includes an RS232 interface so that an external computer can be connected for technical service purposes. • Wash Container . (Shown in Figure 1). The wash container contains the wash fluid that is used[...]
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Page 25
Hardware Components Connectors at the rear of the Opsys MW™ system are shown in Figure 3. Connections for the power cord, dispense pump power, waste bottle level sensor, waste bottle vacuum line and an external computer are made at the rear of the system. Important : Be sure that the vacuum exhaust is not obstructed. Figure 3. Rear View of th[...]
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Page 26
Positioning the Instrument Positioning the Instrument Determine the area where the system will be located. You will need a firm and level area that is approximately 51 cm (20 inches) wide, 62 cm (24 inches) deep, and 40 cm (16 inches) high for the Opsys MW™. 18 Opsys MW™ User’s Guide[...]
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Installing the Wash Head Installing the Wash Head The Wash Head must be located on the Wash Head Arm. To install the Wash Head: 1. Position the Arm over the Purge Reservoir portion of the Plate Holder. 2. Place the Wash Head on the two Arm Pins, being careful to properly seat the Wash Head on the support pins. 3. Level the Wash Head in relation to [...]
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Page 28
Installing the Wash Container Installing the Wash Container The dispense tubing and dispense pump power cable must be connected. To install the Wash Container: 1. Fill the Wash Container with the wash solution that is to be used. Note : The Wash Container contains up to 4 liters. 2. Insert the Pick-Up Filter into the Wash Container and secure t[...]
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Page 29
Installing the Waste Container Installing the Waste Container To install the Waste Container and Foam/Aerosol Trap: 1. Insert the Quick-Connect end of the vacuum tubing on the Foam/Aerosol Trap to the Quick-Connect vacuum connector at the rear of the instrument Figure 3). 2. Screw on the Waste Container cap. 3. Route the waste tubing through the lo[...]
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Page 30
Turning on the System Turning on the System Note : Depending upon local electrical codes, an uninterruptible power system (UPS) may be required in your laboratory. A UPS is not provided with the Opsys MW™ system. 1. Connect the Opsys MW™ to the laboratory electrical supply outlet. CAUTION: The Opsys MW™ instrument m ust be connected t[...]
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The Keypad Opsys MW™ User’s Guide 23 Chapter 3 Configuration The Keypad The keypad is used to access the menus and commands for setting up the Opsys MW™ entering new wash protocols and running the wash protocols. The keypad contains scroll keys , function keys , and alphanumeric key s . The keys on the keypad are shown in Figure 4. 1 ABC DEF [...]
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The Keypad The use of each of the keys is summarized below: Key Purpose Scroll keys The functionality of the scroll keys depends upon what is being displayed. During display of the main menu (page 26), pressing the up or down scroll keys will display successive wash protocol numbers and names (or dashes, if a protocol is not yet named). Horizontal [...]
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The Keypad Key Purpose Alphanumeric Keys The numeral shown on the key is displayed by pressing the key once. If the key is pressed two to seven times rapidly, one of the letters on the key will be displayed. The letter that is displayed depends upon the number of times the key is pressed. Opsys MW™ User’s Guide 25[...]
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Menus Menus The Opsys MW™ menus are hierarchical and are organised by function. There are two main menus: Setup and Program. Menu Purpose SETUP Configure the Instrument PROGRAM Create or edit plate washing protocols * * A protocol includes general information (e.g. the type of microplate to be washed) as well as a listing of the various steps to [...]
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Menus The SETUP Menu Press the function key under SETUP to display the SETUP menu (Figure 5). The SETUP menu contains commands to configure the instrument. 1: TESTNAME SETUP PROGRAM Select Option VERSION CAL. Select Option HEAD PLATE Figure 5. SETUP Menu The PROGRAM Menu Press the function key under PROGRAM to display the PROGRAM menu (Figure 6). T[...]
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Page 36
Configuring the System Configuring the System The Opsys MW™ must be configured before it is operated for the first time. The commands for doing this are contained in the SETUP menu (Figure 7). Use ARROW keys to scroll through the menus. 1: TESTNAME SETUP PROGRAM Select Option VERSION CAL. Select Option HEAD PLATE Figure 7. SETUP Menu The purpose [...]
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Page 37
Configuring the System Specifying the Wash Head The HEAD command allows the installed wash head to be specified: 1. Select SETUP. The display will read: Select option HEAD PLATE 2. Select HEAD. The current wash head selection is displayed in the upper line: Wash head? 8 8 If needed, scroll right to view the remaining selection: Wash hea[...]
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Configuring the System Specifying Plate Parameters The PLATE command allows plate parameters to be specified for up to 10 plate types. When setting plate parameters, a default value is displayed for each of the wash head positions. The default values are factory-set and represent approximate starting values for each wash head position. Note : T[...]
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Configuring the System To specify parameters for a plate ty pe: 1. Obtain an empty plate of the plate type being specified and place it in the plate holder. 2. Select SETUP (if needed). The display will read: Select option HEAD PLATE 3. Select PLATE. The name of the first plate is displayed in the lower line: Select plate 1: - - - - - - - - - -[...]
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Page 40
Configuring the System 7. Enter the dispense height using the keypad and press ENTER. The display will read: Well top? 160 SHOW Note : The default value is displayed. Select SHOW to move the wash head to the default well top position so that the height can be checked. Then, determine the correct value as outlined on page 30. Repeat the procedur[...]
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Page 41
Configuring the System Remember if your protocol requires sweeping, that must first be specified in the plate definition used in the protocol. 11. Select YES to specify a sweep on all aspiration cycles. The display will read: Sweep height? 370 SHOW Note : The default value is displayed. Select SHOW to move the wash head to the default sweep pos[...]
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Configuring the System 15. Enter the desired value using the keypad and press ENTER. The display will read: Select option HEAD PLATE Note : If NO is selected for Bottom wash? (Step 13), the display will read as shown above. 34 Opsys MW™ User’s Guide[...]
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Configuring the System Displaying the Software Version The VERSION command displays the software version that is currently installed on the Opsys MW™ instrument: 1. Select SETUP (if needed). The display will read: Select option HEAD PLATE 2. Scroll right to display the next selection: Select option VERSION CAL. 3. Select VERSION. The [...]
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Configuring the System Specifying the Pump Parameters The CAL. command allows the calibration values for each pump to be specified: 1. Select SETUP (if needed). The display will read: Select option HEAD PLATE 2. Scroll right to display the next selection: Select option VERSION CAL. 3. Select the pump pressure (high or low). The display [...]
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Page 45
Configuring the System Selecting Clean The CLEAN command enables the washer to be cleaned automatically at a specified interval. 1. Scroll right to display the next section: Select option CLEAN 2. Select CLEAN. The display will read: Auto Clean? YES NO 3. Select YES if you want periodic automatic cleaning. The display will read: Interval (Hrs) [...]
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Configuring the System This page is intentionally left blank 38 Opsys MW™ User’s Guide[...]
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Wash Protocol Operations Chapter 4 W ash Protocols Wash Protocol Operations A wash protocol consists of a series of Purge , Move , Soak , Aspirate , Dispense and Fill operations. Purge , Move and Soak can be carried out in any sequence. Aspirate , Dispense and Fill can only be carried out within a Move operation, and there cannot be a Move within a[...]
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Wash Protocol Operations Operation Description Dispense Dispenses a specified amount of fluid into the wells after aspirating the contents of the wells (i.e. wherever a DISPENSE step is added to a protocol, the OPSYS MW will automatically aspirate the wells before dispensing more fluid in order to prevent a well overflow). If a bottom wash is speci[...]
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Creating a New Wash Protocol Creating a New Wash Protocol Specifying the Header NOTE: Pressing CANCEL while creating or editing a protocol will bring you back to the main menu without saving your changes. or (or use keypad) Select one of the 40 protocol numbers. ↓ PROGRAM Select PROGRAM from the main menu. ↓ (keypad) Enter a protoco[...]
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Creating a New Wash Protocol To create a new w ash protocol header: 1. Display the main menu. 1: - - - - - - - - - - SETUP PROGRAM 2. Use the up or down scroll keys to display the protocol number (or use the keypad to enter the protocol number) that is to be created: 6: - - - - - - - - - - SETUP PROGRAM Note : Dashes will be displayed if a prot[...]
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Creating a New Wash Protocol 7. Re-enter the password and press ENTER. The display will read: Select plate 2: DYNEX_1 Note : The plate name that is shown is an example. The actual name will be the plate name that is entered for that plate type during system configuration (see page 30). 8. Use the up and down scroll keys to select the plate type[...]
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Creating a New Wash Protocol 10. Select YES if the total elapsed time for washing any number of strips within a plate is to be constant. The display will read: Select option MOVE PURGE The sequential steps in the wash protocol can now be specified, as outlined on page 45. 44 Opsys MW™ User’s Guide[...]
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Creating a New Wash Protocol Specifying Wash Cy cle Operations The final step in creating a wash protocol is to specify individual operations within the wash protocol. An example of a wash protocol is shown in 45. In this example, the dispense wash pin s are purged, and then a Dispense and Aspirate are carried out twice on each of the 12 strips (co[...]
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Creating a New Wash Protocol To specify a Purge: Note : A purge must be performed outside of a Move cycle. 1. Display the header information for the selected wash protocol, as outlined on page 41. The display will read: Select option MOVE PURGE 2. Select PURGE. The display will read: Fluid volume? 350 3. Use the keypad to enter the volume o[...]
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Creating a New Wash Protocol To specify a Move cy cle: 1. Display the header information for the selected wash protocol, as outlined on page 41. The display will read: Select option MOVE PURGE 2. Select MOVE. The display will read: Start strip? 1 3. Using the keypad, enter the strip number where the move cycle should start. 4. Press ENTER. The [...]
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Page 56
Creating a New Wash Protocol To specify an Aspiration (within a Move): Note : An Aspirate or Dispense must be performed within a Move cycle. 1. Select ASPIRATE. The display will read: Cycles? 1 2. Using the keypad, enter the number of aspiration cycles that are to be to be performed within the MOVE cycle. 3. Press ENTER. The display will read: [...]
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Creating a New Wash Protocol To specify a Dispense (within a Move): Note : A Dispense or Aspirate must be performed within a Move cycle. 1. Select DISPENSE. The display will read: Fluid volume? 350 2. Use the keypad to enter the volume of fluid to be dispensed. Note : A volume ranging from 50 to 999 microliters can be entered. 3. Press ENTE[...]
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Creating a New Wash Protocol 6. Press ENTER. The display will read: Cycles? 1 7. Use the keypad to enter the number of bottom wash cycles to be performed and press ENTER. The display will read: Select option ASPIRATE DISPENSE 50 Opsys MW™ User’s Guide[...]
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Creating a New Wash Protocol To specify a Soak (within a Move): Note : A Soak can be performed either within or outside of a Move cycle. The procedure below describes the procedure for specifying a soak within a Move cycle. The procedure for specifying a Soak outside of a Move cycle is described on page 50. 1. Scroll right to display the next s[...]
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Creating a New Wash Protocol To specify a Fill (within a Move): Note : A Fill must be performed within a M ove cycle. 1. Scroll right to display the next selection. The display will read: Select option SOAK FILL 2. Select FILL. The display will read: Fluid volume? 50 3. Use the keypad to enter the volume of fluid to be dispensed during [...]
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Creating a New Wash Protocol Ending a MOVE Cycle 1. Scroll right to display the next selection. The display will read: Select option SOAK FILL 2. Scroll right to display the next selection. The display will read: Select option END LOOP 3. Select END LOOP. The display will read: Select option MOVE PURGE Opsys MW™ User’s Guide 53[...]
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Creating a New Wash Protocol To specify a Soak (outside of a Move): Note : A Soak can be performed either within or outside of a Move cycle. The procedure below describes the procedure for specifying a soak outside of a Move cycle. The procedure for specifying a Soak within a Move cycle is described on page 47. 1. Scroll right to display the ne[...]
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Creating a New Wash Protocol To specify the End of the wash protocol: 1. Scroll right to display the next selection. The display will read: Select option SOAK END 2. Select END. The display will read: 1: NAME_01 SETUP PROGRAM Opsys MW™ User’s Guide 55[...]
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Modifying a Wash Protocol Modifying a Wash Protocol An existing wash protocol can be modified by using many of the same commands that are used to create a wash protocol. To modify a wash protocol: 1. Display the main menu: 1: NAME_01 SETUP PROGRAM 2. Use the up and down scroll keys to select the wash protocol to be modified (or enter the number usi[...]
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Modifying a Wash Protocol 6. The cursor is positioned under the first character of the name. If the name is to be changed, enter the name using the keypad and press ENTER. The first step of the wash protocol will be displayed: MOVE 1-12 1 Cycles DELETE EDIT Note : The example above shows the MOVE operation as the first step in the protocol.[...]
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Modifying a Wash Protocol To modify a wash operation: 1. Select EDIT while the operation is displayed. The first step in the operation is displayed: Start strip? 1 Cycles Note : The actual step that is displayed depends upon the operation being modified. 2. Modify the operation, using the same procedures as when creating a new wash protocol. MO[...]
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Page 67
Modifying a Wash Protocol To delete a wash operation: 1. Select DELETE while the operation is displayed. The operation is removed from the wash protocol. Note : If a MOVE operation is deleted, all operations within the MOVE (i.e., Aspirate , Dispense , Soak and Fill ) are also removed. Opsys MW™ User’s Guide 59[...]
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Modifying a Wash Protocol To insert a wash operation: 1. Scroll right while the operation that the new operation is to precede is displayed: MOVE 1-10 1 Cycles INSERT Note : The inserted operation will take place immediately before the operation that is displayed. 2. Select INSERT. The display will read: Select option MOVE PURGE 3. Sele[...]
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Copying a Wash Protocol Copying a Wash Protocol An existing wash protocol can be copied to another program number. If a wash protocol is already in that location, it will be overwritten. To copy a wash protocol: 1. Display the main menu: 1: NAME_01 SETUP PROGRAM 2. Use the up and down scroll keys to select the wash protocol to be modified (or enter[...]
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Copying a Wash Protocol 7. Using the keypad, enter the number of the wash protocol that the commands are to be copied to. If a wash protocol already exists at that location the display will read: Overwrite test? NO YES NO 8. Select YES. The display will read: 7: NAME_04 SETUP PROGRAM 62 Opsys MW™ User’s Guide[...]
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Preparation Chapter 5 W ashing a Plate Once the parameters for at least one plate type and one wash protocol have been set, the system can be used to wash a plate. Preparation The only preparation that is required is to check the fluid levels in the Wash Container and the Waste Container. Service the containers if necessary. To empty the waste cont[...]
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Page 72
Preparation To refill the wash container: 1. Separate the dispense line quick-connector in the cap of the Wash Container. 2. Unscrew the cap of the Wash Container and lay it and the Pick- Up Filter on a clean paper towel. 3. Fill the Wash Container with the wash fluid that is being used. Note : If the wash solution is being changed, discard the[...]
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Page 73
Washing a Plate Washing a Plate A plate is washed by positioning it on the carrier block, selecting the wash protocol to be run, and pressing the START key. To wash a plate: 1. (If needed) Turn on the instrument. The display will read: 1: TESTNAME SETUP PROGRAM 2. Place the microplate on the plate carrier. Note : Be sure that the plate position[...]
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Page 74
Washing a Plate This page is intentionally left blank 66 Opsys MW™ User’s Guide[...]
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Routine Maintenance Procedures Chapter 6 Routine Maintenance Routine Maintenance Procedures The Opsys MW™ Microplate Washer is constructed from materials that resist chemical attack. If a spill occurs, it should be cleaned up as soon as possible using a cloth moistened with a mild laboratory detergent (If needed, dilute the laboratory detergent a[...]
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Page 76
Routine Maintenance Procedures • Replace the dispense (Wash) tubing. • Replace the aspirate (Waste) tubing. Note : The dispense tubing and aspiration tubing may need to be replaced more frequently than every six months, depending upon the frequency of use and the severity of operating conditions. Contact DYNEX Technologies for information o[...]
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Page 77
Cleaning and Decontamination Cleaning and Decontamination The Opsys MW™ is constructed from materials that resist chemical attack. Spills should be cleaned as soon as possible. If you need to decontaminate the Opsys MW™ instrument (for example, before servicing the instrument), clean the system and then decontaminate it as described below. [...]
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Page 78
Cleaning the Containers Cleaning the Containers Procedures for emptying, cleaning and refilling the Wash Container and the Waste Container are described below. To empty and clean the waste container: 1. Unscrew the cap of the Waste Container. Lay the cap and level sensor assembly on a paper towel. 2. Discard the waste in accordance with local regul[...]
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Page 79
Replacing the Tubing Replacing the Tubing To replace the dispense tubing: 1. Separate the dispense line quick-connector in the cap of the Wash Container. Unscrew the cap, lay it and the Pick-Up Filter on a clean paper towel. 2. Discard the contents of the Wash Container. Thoroughly rinse the container with deionized water and let it dry. 3. Disconn[...]
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Page 80
Replacing the Tubing To replace the vacuum tubing: Note : This procedure describes replacement of the vacuum tubing connecting the Waste Container to the Foam/Aerosol Trap. The vacuum tubing connecting the Foam/Aerosol Trap to the rear of the instrument should not require changing. 1. Unscrew the Cap of the Waste Container, lay it and the level[...]
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Page 81
Replacing the Tubing To replace the waste tubing: 1. Unscrew the Cap of the Waste Container and lay it with the level sensor assembly on a clean paper towel. 2. Discard the contents of the Waste Container in accordance with local regulations. Thoroughly rinse the container with deionized water and let it dry. 3. Disconnect the waste tubing from the[...]
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Page 82
Pump Calibration Pump Calibration Each Opsys MW™ washer manifold, or head, (e.g., 1 x 8, 1 x 12) has three calibration values stored in memory. These values are calculated and stored during manufacture at DYNEX Technologies. When the Opsys MW configuration is changed from one manifold to another, the corresponding calibration settings will be rec[...]
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Page 83
Pump Calibration 8. If the dispensed volume is too high, decrease the pump speed using the SETUP/CAL function (refer to page 36). • A pump speed change of -10 units roughly corresponds to a well volume change of -10µl. 9. If the dispensed volume is too low, increase the pump speed using the SETUP/CAL function (refer to page 36). • A pump speed[...]
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Operational Problems Chapter 7 T roubleshooting Operational Problems Problems that may occur during normal operation of the Opsys MW™ are described below. For each problem, the symptom, probable cause and the means for resolving the problem are shown. Symptom Probable Cause Resolution The instrument fails to power up. The cord is not connected. C[...]
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Page 86
Operational Problems Symptom Probable Cause Resolution Liquid is not dispensed from the dispense pins (continued). The wash protocol is incorrectly defined. Check the system configuration and wash protocol for the number fluids and fluid volume. The dispense tubing or the dispense pump power supply is not connected. Verify that the dispense tubing [...]
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Page 87
Operational Problems Symptom Probable Cause Resolution Dispense volumes are incorrect. The dispense pins are leaking. Check the pins, seals and tubing for leaks. The dispense pressure is low. Check the seals and tubing for leaks. Check for air leaks. Reseat the tubing in the dispense valve. The dispense pins are clogged. Remove the wash head assemb[...]
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Page 88
Operational Problems Symptom Probable Cause Resolution Aspiration is not complete. (continued) Hydrophobic filter may be obstructed. Replace filter. High Waste Level is displayed. The Waste Container is full. Empty the Waste Container (see page 70). The waste level sensor cable is not plugged in. Connect the waste level sensor cable (see page 17). [...]
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Page 89
Service Procedures Chapter 8 Service Service Procedures The only user service procedures that can be carried out for the Opsys MW™ are listed below: • Removal and replacement of the wash head assembly • Cleaning of the wash head assembly To remove the wash head assembly: 1. Loosen the two thumbscrews at the front of the Arm/Head assembly. 2. [...]
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Page 90
Spare Parts Spare Parts Accessories 13001580 Plate Holder Assembly 13001610 8-Way Head Assembly 13001630 12-Way Head Assembly 352104000 Cleaning Wire, 0.040” 352101800 Cleaning Wire, 0.018” 91000051 User Manual 91000070 OPSYS MW Quick Set Up Instructions 23501800 Bottle Wide Mouth (2120-0010, Nalgene, Wide Mouth Bottle w/Cap) 029006501 Jar, Aer[...]
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Page 91
Returning a Washer for Service Returning a Washer for Service If the instrument must be returned for service, it must be cleaned and decontaminated if it has been in contact with potentially infectious body fluids (including human blood), pathological samples, or toxic or radioactive materials. Note : Refer to page 69 for cleaning and decontami[...]
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Page 92
Returning a Washer for Service EQUIPMENT IN TRANSIT IMPORTANT: Please include a copy of this form with each instrument. If your instrument contains a hard drive, please reta in back-up copies of any stored files. Failure to do so may result in the loss of those files . Return Authorization Number: ___________ Contact Technical Service, DYNEX Techno[...]
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Contact DYNEX Technologies Contact DYNEX Technologies DYNEX Technologies 14340 Sullyfield Circle Chantilly, VA 20151 USA Tel: 703-631-7800 Toll Free: 800-288-2354 Fax: 703-803-1441 www.dynextechnologies.com Opsys MW™ User’s Guide 85[...]
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Page 94
Index Index as required maintenance, 68 ASPIRATE, 39 aspiration height , 4 entering, 32 bottom wash specifying, 34 bottom wash height , 4 CAL. command, 36 calibration prototcol , 74 cleaning , 69 Wash Container, 70 Waste Container, 70 configuration, 28 connections, 17 daily maintenance, 67 data bits, 9 decontamination, 69 DISPENSE, 39 dispense heig[...]
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Page 95
Index variable strips, 41 warning labels, 11 Wash Container, 5, 16 cleaning, 64 installing, 20 refilling, 64 wash fluid, 5 wash head, 3, 15 cleaning, 81 installing, 19 positions, 4 removal, 81 replacement, 81 types, 3 wash pins, 3 wash pins, 3 wash protocol copying, 61 deleting, 59 inserting, 60 modifying, 56 operations, 39, 45 wash protocols progr[...]