Hach DR 5000 manuel d'utilisation

DOC022.53.00654 DR 5000 USER MANUAL January 2008, Edition 2 © HACH Company , 2007–2008. All rights reserved. Printed in Germany .[...]

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3 T able of Content s Section 1 Specification s ............ ................ ................ ................ ............. ................ ................. ................ .... .......... 7 Section 2 General Information ..... ................ ................ ................. ................ ................ ................ ...............[...]

4 T able of Content s 5.4 Stored Programs ....... ................ ................ ................ ................... ................. ................ ......... ............. ............. 42 5.4.1 Select a saved test/method; enter user-specific basi c data ............. ............. ................ ................ .... ...... 42 5.4.2 Stor[...]

5 T able of Content s 6.4 Single Wavelength (absor bance, concentration and transmittance measurements) .... ................... ................ 87 6.4.1 Set up single wavelength mode ........... ................ ................ ................ ................ ................ ... ................ 87 6.4.2 Take single wavelengt h measurements ([...]

6 T able of Content s[...]

7 Section 1 S pecifications S pecifications are subjec t to change without notice. Performance specificatio ns Operating mode T ransmittance (%), Absorban ce and Concentration Source lamp Gas-filled T ungsten (visible) and Deute rium (UV) W avelength rang e 190–1 100 nm W avelength accu racy ± 1 nm in W avelength Range 200–900 nm W avelength r[...]

8 Specifications[...]

9 Section 2 General Information 2.1 Safety information Please read this entire manual befo re unpacking, setting up o r operating this equipment. Pay attention to all danger , warning and caution statem ents. Failure to do so cou ld result in serious injury to the operator or damage to th e equipment. Make sure that the protection prov ided by this[...]

10 General Information 2.1.3 Class 1 LASE R A Class 1 LASER is installed in this instrument. Class 1 LASERS are produc ts where the rad iant power of the LAS ER beam accessible (the accessible emissi on) is always below the Maximum Permissible Exposure value. Therefore, for Class 1 LASERS the output power is below the level at which it is believed [...]

11 General Information 2.2 Overview of product The DR 5000 S pectrophotometer is a complete scanning UV/VIS spectrophotometer with a wavelength r ange of 190 to 1 100 nm. The instrument comes with a complete set of application programs a nd multi-language support. The DR 5000 S pectrophotome ter contains the following application modes: S tored Pro[...]

12 General Information[...]

13 Section 3 Inst allation WA R N I N G Electrical and F ire Hazards. Use only the pr ovided power supply . Only qualified per sonnel should conduct the tasks described in this sect ion of the manual. 3.1 Unp ack the instrument The DR 5000 S pectrophotometer comes packaged with the following items: • DR 5000 S pectrophotometer • Mains power cab[...]

14 Inst allation 3.3 Connection The DR 5000 has three USB interf aces as a standard feature, located on the back of the instrument ( Figure 1 ). The USB T ype A interfaces are used for communications with a printer , USB memory stick or ke yboard. A USB memory stic k is used to update instrument sof tware. The USB T ype B interface is used for comm[...]

15 Inst allation 3.4 Cell comp artment, Multi Cell Ho lder , 100 mm Cell Adapter (optional) The DR 5000 S pectr ophotometer comes equippe d with a Multi Cell Holder ( Figure 3 on page 16 ) which is the S tandard Holder , provided with each instrume nt . The Multi Cell Holder can accommodate the following cell types: • 10 mm square cells • 20/50[...]

16 Inst allation 3.4.1 100 mm adapter (optional) Figure 3 Multi Cell Holder (T op and Bottom) 1 10 mm square cell 4 1“ round cell 2 20 mm rectangular cell 5 1“ square cell 3 50 mm rectangular cell Figure 4 100 mm Cell Adapter 1 100 mm rectangular cell[...]

17 Inst allation 3.4.2 Inst allation Multi Cell Holder Assembly of the Multi Cell Holder: 1. Open the cell compartmen t. 2. Identify the correct opening for the selected cell ty pe in the Multi Cell Holder . 3. Insert the Multi Cell Holder in the cell compartment in such a way that the nam e of the selecte d cell type can be read directly and the c[...]

18 Inst allation 3.5 Beam p ath Figure 6 shows the be am path of the DR 5000: Figure 6 Be am path 1 Deuterium lamp 10 Exit slit 2 Lamp selection mirror 11 Cell compartment 3 T ungsten l amp 12 Lens 4 Mirror 13 Round cuvette/vial compartment 5 Grating 14 Measurement element 6 Reference element 15 Monochromator 7 Mirror 16 Mirror 8 Sp l i t t e r m i[...]

19 Section 4 S t a rt Up 4.1 Power the ins trument on and off 1. Plug in power supply . 2. Close the empty cell compartment. 3. T urn the instrument on by pressing the power switch on the back. Note: Do not turn the instrument off an d on in rapid succession. Always wait about 20 seconds before turning th e instrument on again, otherwise the electr[...]

20 Star t U p[...]

21 Section 5 S t andard Operations 5.1 Overview 5.1.1 Tip s for the use of the touch screen The entire screen is to uch-activate d. T o make a se lection, pres s the screen with a fingernail, fingertip, pencil eraser or a stylus. Do not press the screen with a sharp object, such as the tip of a ball point pen. • Do not place anything on top of th[...]

22 St an dard Operations 5.1.3 Main Menu A variety of modes may be selected fr om the Main Menu. The following table br iefly describes each menu option. T a ble 2 Main Menu options Option Function Stored Programs (HACH LANGE Programs) S tored programs are pre-programmed methods that make use of HACH reagents and LANGE cuvette tests and pipette tes[...]

23 St andard Operations 5.2 Instrument Setup mode 1. Select Instrument Setup in the Main Menu. A selection of functions appears in order to configure the functions of the instrument. This display appears when the mult i-cell holder is installed or the 13 mm round cuvette/vial comp artmen t is use d. If the imstrument recognizes a Sipper module or a[...]

24 St an dard Operations 5.2.2 Sample ID Use this option to ente r up to 30 Sample Identification tags (up to 13 character s each) into th e instru ment. Sample IDs can be used to specify the sample location or ot her sample specif ic information. 1. Press Sample ID in the Instrument Setup. 2. Press New to enter a new Sample I D. 3. Use the alphanu[...]

25 St andard Operations There are two ways to enter or change a Sample ID in the Measurement Mode: Option 1 In the result display , press Options > More > Instrument Setup . If a Sample ID is already assigned, sele ct the Sample ID symbol in the result display . Option 2 With an USB keyboard (with US ke yb oard layout) or a scanner a Sample I[...]

26 St an dard Operations 2. Select Long to change the number of audio signa ls. Use the alphanumeric keyp ad to enter/specify the number of audio sign als (4–25). Note: A high number of audio signals increases the duratio n of the tones and a small number of audio signals reduces the duration o f the tones. 3. Press OK to confirm. The s elected n[...]

27 St andard Operations 5. Select the length of time the lamp will be switched on. Note: After this period of time the lamp automatically turns off, after no measurement has been made o utside the corresponding range (UV/VIS). 6. T ouch OK to confirm. 7. To u c h UV Switch key to select the wavelength value be tween 320 nm and 360 nm, at which the [...]

28 St an dard Operations When the lamp is warmed up and re ady , the UV icon stops flashing. Note: If both lamps are On, the icon UV -VIS is displayed in the selected Measurement Mode. Note: Alternatively you can touch the UV- or VIS-Lamp icon on the measurement screen to change the actu al setting.[...]

29 St andard Operations 5.2.6 PC and printer The instrument is provided with 3 USB interfaces, which are located on the back of the instrument (see Figure 1 on page 14 ). These interfaces c an be used for expor ting data and graphics to a pr inter , updating dat a and for dat a communication to a personal computer . These interfaces can be used for[...]

30 St an dard Operations Printer Setup: • Resolution: Print quality • Paper: Paper size Note: If an optional Thermal Pri nter is connected, the function "Auto Send" on/off is available. 4. Select Auto-Send: On to send all measured dat a automatically to the Thermal printer . Note: The option Auto-Send is not available for any othe r p[...]

31 St andard Operations 7. Press Paper to select the paper size. Select between • Letter • Legal • Executive • A4 8. Press OK to confirm. Note: Press OK again to return to the PC & Printer menu. 5.2.6.2 Print dat a 1. Press Recall Dat a in the Main Menu. 2. Select the data sou rce, where the data to be printed are stored. 3. A list is d[...]

32 St an dard Operations 4. Select Auto-Send: On to send all measured dat a automatically to the PC. Note: If Auto-Send: Off is selected, the PC & Printer icon must be pressed, in order to send data to the PC. Note: The remote funct ion is onl y fo r monitoring the data transfer . 5.2.7 Password The Password menu cont ains a variety of security[...]

33 St andard Operations 4. Press Security List to lock various functions for unauthorized users. 5. Highlight the d esired funct ions to cont rol. 6. Confirm the Security List wi th OK to return to the Password menu. 7. Press On to highlight the new settings of the Security List. 8. Enter the n ew Password again to conf irm. 9. Press OK to return t[...]

34 St an dard Operations 3. Press Off to deactivate the settings of the Security List. 4. Press OK to return to Instrument Setup. Note: Use this function to dele te the former Password or to enter a new one.[...]

35 St andard Operations 5.3 S tore, recall, send and delete dat a 5.3.1 The Dat a Log The Data Log will store up to 1000 readings taken in the modes: S tored Pro grams, User Programs, Favorite Programs, Single W avelength and Multi W avelength . A complete record of the analysis is stored, including the Date, T ime, Res ults, Sample ID, and Operato[...]

36 St an dard Operations 4. The function Filter Settings is used to search for specific items. 5. Highlight On to turn on the filt ers to select data by • Sample ID • Operator ID • St a r t D a t e • Parameter or any combination of the four . 6. Press OK to confirm the se lection. The ch osen items are liste d. 7. Press Vie w De tai ls to g[...]

37 St andard Operations 5.3.1.4 Delete stored dat a from the dat a log 1. Press Recall Dat a in the Main Menu. 2. Press Dat a Log>Options>Delet e . 3. Highlight Single Po int or Filtered data or All dat a and press OK to confirm. Note: The number in parentheses is the total number of data set s assigned to this selection.[...]

38 St an dard Operations 5.3.2 S tore, recall, send and delete dat a from wavelength scan and time course The instrument can store 20 W avelength Scans and 20 T ime Course Data sets.The dat a can be stored manually at the user's discretion after viewing the da ta. 5.3.2.1 Dat a storage from waveleng th scan or time course 1. Press the Stor e i[...]

39 St andard Operations 2. Press Graph to look at det ails. Note: Press V iew Summary to return to the Recall Data list. 3. Press Ta b l e to look at deta ils. Note: Press V iew Summary to return to the Recall Data list. 5.3.2.3 Send dat a from wavelength scan or time course There are two ways to recall sent dat a to a USB memory stick, printer or [...]

40 St an dard Operations • When a printer is conn ected, select how to send the dat a to the printer (graph, t able or both grap h and table). • When a USB memory stick is connected, the files will be automatically sent as CSV f iles (Comma Separ ated V alue) to a file ”WLData“ (W avelengt h Scan Data) or ”TCData“ (T ime Cour se Data) t[...]

41 St andard Operations 5.3.2.4 Delete stored dat a from wavelength scan or time c ourse 1. Press Recall Dat a from the Main Menu and then Wa v e l e n g t h Scan or T ime Course or Options>M ore>Recal l Data . A listing of the stored data is displayed . 2. Highlight any dat a to delete. 3. Press Delete in the Options menu and press OK to con[...]

42 St an dard Operations 5.4 S tored Programs The instrument contains more than 200 programmed procedures. They can be accessed th rough the St ored Programs menu. 5.4.1 Select a saved test/method; enter user-specific basic dat a 4. Press Stored Programs in the Main Menu to view an alphabetical list of stored programs with pr ogram numbers. The S t[...]

43 St andard Operations Store Off/On With the Store On setting, all measu rement data ar e stored automatically . With the Sto r e O ff setting, no measurement data are stored. % T rans/Conc/Abs To switch to % transmittance, conc entration o r absorbance readi ngs Send Dat a icon / Send Dat a T o send Data to a printer , comp uter or USB memory sti[...]

44 St an dard Operations 5.4.3 Use of program timers Some procedures do not r equire the use of timers. Other procedures require se veral timers. T hese timers are pre-progr ammed into e ach Stored Program , alo ng with a description of the activity to be performed durin g the timed period. 1. Press the Tim er icon o n the display . 2. Press OK to [...]

45 St andard Operations 5.4.4.1 T ake single wavelength measurement s (single reading) 1. Insert the blank cuve tt e into the cuvette/ sample cell holder. Press Zero . Note: The Read key is only active after the zero measurement has been carried out. 2. Insert the samp le cuvette into the cuve tte/sampl e cell holder. Press Read . Note: For data st[...]

46 St an dard Operations 3. Press Return to return to the result display . 4. Confirm ’S tore present reading again?’ to save the current measured value with the dilu tion factor . Note: When a dilution is in effe ct, the dilution icon will appe ar on the display . Note: If using undiluted samples, set the dilution factor off ag ain. 5.4.6 Run [...]

47 St andard Operations 5. Press Adjust to enable the S tandard Adjust. The S tandard Adjust icon will appear . Note: The adjustment must be within ce rtain limits, which vary with each program. The allowable perce ntage is shown af ter "Adjustment“. Note: When a S tandard Adjust is in effect, the S tandard Adjust icon will appear on the dis[...]

48 St an dard Operations 5.4.8 Run a reagent blank Some of the stored test s/meth ods include the "Reagent Blank" function. This enables the reagen t blank value to be measur ed and then taken into accoun t in calculating the measurement result. Measurement/analys is of a reagent blank: 1. Prepar e the test/method in accordance with th e [...]

49 St andard Operations 5.4.9 Analysis of samples 1. Press S tored Programs and select a progra m. 2. Insert the blank cuvette/cell into the cuvette/sample cell holder . 3. Press Zero . 4. Remove blank cuvette/cell and in sert sample cuvette/cell into the cell compartment. 5. Press Read . The result will be displayed. 6. For dat a storage, see sect[...]

50 St an dard Operations 5.5 Barcode Programs A special barcode reader in ce ll comp artment #1 automatically reads the barcode on the 13 mm cu vette/vial as the cuvette/vial completes a single rot ation. The instrument uses the barcode identification to automatically set the correct wa velength for the analysis and calculates th e result immed iat[...]

51 St andard Operations 5.5.1 Complete a barc ode 13 mm test/vial Note: The cell compartment must be closed before a mea surement is made! 1. The instrument needs to be at the Main Menu. 2. Prepare the barcode 13 mm cuve tte/vial in accordance with the working procedure and insert the cuvette/vial in ce ll compartmen t #1 and close the cell compart[...]

52 St an dard Operations 1. In the result display , press Options>More>Chemical Form . A list of available evaluation fo rms appears. 2. Select the required chemical form an d press OK to confirm. Note: The selected chemical fo rm is displayed, but does not become the default. T o change the defau lt, see section 5.5.3.1 . 5.5.3.1 Change of t[...]

53 St andard Operations 5.5.5 Sample blank T urbidity and color in t he sample matrix can fa lsify the results of a photometric analysis. The interference factors come from the sample or are cr eated by reactions with the reagents. The influence of tur bidity and/or color can be eliminated or reduced by taking a sample blank reading. In the barcode[...]

54 St an dard Operations procedure. Other barcod e tests use the reagent test vials to determine the sample bla nk. Please refer to the Hach procedure for method-spe cific instructions. 5.5.6 Up date/edit barcode test s 5.5.6.1 Manual up date of a barcode test Using the data provided in th e barcode, the instrument automatically set s the measureme[...]

55 St andard Operations Enter the V ersion 1. Highlight the line cont aining V ersion and press Edit . Use the alphanumeric keyp ad to enter the name indica ted in the working procedure. 2. Press OK to confirm. Enter the Measurement Process For detaile d information on enterin g the measurement process, see section 6.1.2 on page 72 . Check the work[...]

56 St an dard Operations Enter the V ariables (Factors, W avelength and Conversion Factors) For det ailed information on entering the V ariables, see section 6.1.2 on page 72 . Check the working pr ocedure in adva nce to determin e whether this point needs to be cha nged. 1. Highlight the Va r i a b l e s line in the data over view and press Edit .[...]

57 St andard Operations 5.5.6.2 Up date an existing barc ode test Note: Only in very few cases does the re vision of a test require all test specifications to b e updated. Option 1: Manual up date of test data 1. The instrument needs to be at the Main Menu. 2. Insert the blank or sample cuvette/vial (depending on the working procedure) in cell comp[...]

58 St an dard Operations 5.5.6.3 Program a new test 1. The instrument need s to be at the Main Menu. 2. Insert the sample cuve tte/vial or blank (depending on the working procedure) into th e cell compartm ent #1 and close the cell compartment. 3. After "Progra m not available", a data overview is displayed, including the specifications o[...]

59 St andard Operations 6. Press OK to return to the System Checks menu. Note: When the instrument software has been updated, a prompt to restart the instrument is displayed.[...]

60 St an dard Operations[...]

61 Section 6 Advanced Operations 6.1 User Programs User programs provide the op portunity to complete "made to measure" analysis. The User Progra ms database is em pty when the instrum ent leaves the factory and is used to acco mmodate programs cre ated by users specific need s. Here are a few examp les of entrie s: • Programming of use[...]

62 Advanced Operations Program Numb er: S pecific test number , with whic h the progra m can subseq uently be called up from the selection list in the User Programs menu or the Favorites menu. 2. Use the alphanumeric keyp ad to enter a program number between 950 and 999. The lowest available number a ppears automatically . 3. Press OK . Note: If th[...]

63 Advanced Operations 6.1.1.1 Single wavelength settings If the Single Wavelength m ode is selected, the following parame ters can be defined: Unit s: Select the required unit from the list and press Next . Note: Units of measure not included in this list can be added in the edit program under Program Options , Edit . Select Units , Edit and th en[...]

64 Advanced Operations Chemical form: Enter the chemical formula used in the display to represent the analysis parameter . Use the alphanumeric keyp ad to enter the chemical form and press Next to enter Calibration settings. 6.1.1.2 Multi wavelength settings If the Multi W avelength mode is se lected, the follo wing p aramete rs can be defined: Uni[...]

65 Advanced Operations List of available absorbance for mulas A 1 is the absorbance at waveleng th 1, A 2 is the absorbance at waveleng th 2 and so on K 1 is the factor at wavelength 1, K 2 is the factor at wavelength 2 and so on If a subtraction has to be completed, the factor s can be entered with a minus sign. Wa v e l e n g t h λ x : Press a ?[...]

66 Advanced Operations Chemical form: Enter the chemical formula used in the display to represent the analysis parameter . Use the alphanumeric keyp ad to enter the chemical form and press Next to enter Calibration settings. 6.1.1.3 Calibration settings for single and multi wavelength mo de A method is calibrated by dete rmining the absorbance valu[...]

67 Advanced Operations 2. T o enter the standard concentrations and cor responding absorbance value s in the displayed t able, press the " + " symbol. Use the alphanume ric keypad to enter the values. Press OK and enter th e corresponding absorbance value. Press OK . The entered dat a are displayed in the t able. Repeat the sequence for e[...]

68 Advanced Operations Calibration by r eading st andards 1. Press Read St andards and press Next . 2. T o enter the standard con centrations in the displa yed table, press the " + " symbol. Use the alphanumeric keyp ad to enter the standa rd concentration. Press OK . 3. Press the "+" s ymbol agai n (see arrow ) and ente r the n[...]

69 Advanced Operations 9. Press Force 0 to change the setting from Of f to On . The curve then passes throug h the origin of the coordinate system. Note: This may have an adverse effect on the correlation coefficient (r 2 ). 10. Press Ta b l e to display the table again. 11 . When the tab le has been completed and the curve type has been chosen, pr[...]

70 Advanced Operations 6.1.1.4 Store a user program The input of the basic data is complete. An overview of the variable program d ata is displayed. 4. T o enter more specifications or change existing ones, highlight the appropriate line and press Edit . 5. Select St or e to save the user program. 6. Press the PC & Printer icon to send the prog[...]

71 Advanced Operations Timer 1 / T imer 2 / Timer 3 / T imer 4: This function can define time interv als for up t o four timers. T imer designations such as Shake, W ait and Swirl can be assigned. 1. Highlight the appropriate line in the overview of the program data an d press Edit . 2. The timers are activated or deact ivated with the check boxes [...]

72 Advanced Operations 6.1.2 Free programming program type Free Programming is an advan ced option for entering original user-devel oped methods. When the Free Programming option is selected, an overview of the specifications of the progra mmed test is displayed. Each inpu t option can be modified to d evelop the user method. Refer to Table 10 for [...]

73 Advanced Operations • Should individual program seq uences be repeate d? The element s of a measuring sequence, such as zero and sample measurement s and the timer(s) (reaction ti mes, waiting times, etc.) are individually defined. 6.1.2.2 Enter a new element of a me asuring sequence Important Note: Each co mponent of the measurement process m[...]

74 Advanced Operations Process Ti mer key 1. Press the Process Timer key to enter any wait ing, reaction or handling tim es that have t o be taken into acc ount. Use th e alphanumeric keyp ad to enter the time. Press OK and co nfirm the input by pressing OK again. Note: This time is integrate d into the measurement process. Note: The entered measur[...]

75 Advanced Operations Delete an element of a measuring sequence Select the appropriate line and press Delete . The element is deleted. Insert an element of a measur ing sequence Select the line in the mea suring sequence where the insertion is be made and press New . A new element can be entered at the selected position. When the inpu t is complet[...]

76 Advanced Operations Refer to Table 11 for detailed information on the Edit formula keys. Note: The evaluation formula is built up successively in the disp lay in accordance with the input. Note: The arrow key del etes the most recent ly entered element of the formula. T able 1 1 Edit formula key descriptions Screen Key Description Abs/V ariables[...]

77 Advanced Operations Enter the ne xt calibration formula (C2 or Cn) 5. Highlight C2: Off a nd press Edit . 6. Select C2: Off again and press Edit . The display switches to C2: On . 7. Highlight the next line C2 = to define the formu la and press Edit . In addition to the keys described in Table 11 , on ly one other function is available: +– ÷ [...]

78 Advanced Operations Select Conc. key If an already defined formula, in this case C1, is to be take n into account in the formula for C2, p ress Select conc. Enter the nu mber of th e formula (e.g. 1 for C1 ) and pres s OK to confirm. Cn can now be linked with a mathematical operation. Note: The Cn concentrations that are to be calcu lated are nu[...]

79 Advanced Operations 6.1.3 Select a user program 1. Press User Programs in th e Main Menu to view an alphabetical list of user pr ograms with progra m numbers. The User Programs list will appear . Note: Use the scroll bar to scroll throug h the list quickly . Note: Highlight the selection by pre ssing it or press Select by Number to search for th[...]

80 Advanced Operations 6.1.4.1 Add to Favorites 1. Press Add to Favorites and press OK to confirm. The program is ad ded to the Favorites. 6.1.4.2 Edit 1. Press Edit and pres s OK to confirm. An overview of the specificati ons of the programm ed test is displayed. More in formation about th e input options is pr ovided in the section 6.1.2 on page [...]

81 Advanced Operations 6.2 Favorite Programs The most frequently used tests/methods in the St ored Programs menu and the User Programs menu can also be added to the list of favorites to simp lify their selection. T o add Stored Programs and/or User Programs to the favorites list or the favorite prog rams, see section 6.1.4 on page 79 . 6.2.1 Recall[...]

82 Advanced Operations 6.3 S t andard Addition – monitoring/check ing result s The accuracy of measured values (their correspondence with the actual concen tration of the analyte in the sample) and their precision (corre spondence of the measurement re sults obt ained from several samples containing the same concentration of the test analyte) can[...]

83 Advanced Operations e. Does the te st require t he sample to be at a cer tain temperature? f. W as the pH of the sample in the correct range? g. Is the pipette volume correct? 2. Check the used reagent s by repeating the stan dard addition procedure with fr eshly prepared re agents. If none of these measures reso lves the problem, please cont ac[...]

84 Advanced Operations Peak volume/samp le volume methods 1. Select Stored Progr ams in the main menu. Select the requir ed program. 2. Press Star t . 3. Analyze a sample without added st andard solution in accordance with the instruction s in the Procedures Manual. When the measurement is complete, leave the sample cuvette/cell in the cuvette/samp[...]

85 Advanced Operations 6. Press the key of the value to change it. Use the alphan umeric keypad to change the value. Press OK to confirm. 7. Press the keys to enter the st andard addition volumes. Use the alphanumeric keyp ad to enter the new dat a and press OK . Description of the t able of readings • The first column sh ows the standard a dditi[...]

86 Advanced Operations 11 . After all th e standard addition solu tions have been measured, press Graph . The regressio n line through the standard a ddition data points is displayed. The correlation coef ficient r 2 indicates how c lose the data points are to th e line. If the correlation co efficient = 1, the curve is linear . The concentration s[...]

87 Advanced Operations 6.4 Single W a velength (absorbance, concentration and tran smitt ance measurement s) The Single W avelength mode can be used in thr ee ways. For sample measurement s at a single wavelength, the instr ument can be programmed to measure the absorba nce, % transmittance or concentration of the analyte . Absorbance me asures the[...]

88 Advanced Operations Concentration factor: 1. Press Concentration Factor: Off in the Options m enu. Press On to highlight this feature. 2. Press the ”Factor“ key and use the alphan umeric keypa d to enter the f actor by w hich absorban ce reading s are to be multiplied. Press the ”Unit“ key to select the units for concentration measuremen[...]

89 Advanced Operations Reading mode: 1. T o highlight th e required mode, start by p ressing Reading mode . 2. Select the required mode , then press OK , then Return to return to the result display . 6.4.2 T ake single w avelength measurement s (single reading) 1. Insert the blank cuvette /cell into the cuvette/sample cell holder . Press Zero . Not[...]

90 Advanced Operations 6.5 Multi-W avelength mode – measureme nt s at more than one wavelength In the multi-wavelength mode, absorbance values can be measured at up to four wavelengths and the result s can be mathematically processed to obt ain sums, dif ferences an d relationship s. Absorbance measures the amount of light ab sorbed by the sample[...]

91 Advanced Operations λ / Absorbance formula: 1. Press Absorbance For mula . 2. The formula selected in the to p key determines the number of wavelength and coef ficent keys that will appear below . T o change the absorbance formula, pr ess the top key , select a formula from the displayed list and press OK . When a new formula is selected, the n[...]

92 Advanced Operations 3. T o change a wavelength, press one o f the ” λ x:“ keys. Enter the desired wavelength coef ficient into the numeric keypad . Press OK to confirm. 4. T o change a coefficient, press one of the ”K X :“ keys. Enter the desired coef ficient into the numeric keypad. Press OK to confirm. Note: The instrument allows entr[...]

93 Advanced Operations 6.5.2 Complete a measurement in the multi wavelength mode 1. Insert the blank cuvette /cell into the cuvette/sample cell holder . Press Zero . Note: The Read key does not be come active until th e zero measurement has been completed. 2. Insert the sample cu vette/c ell into the cuvette/sample cell holder. Press Read . Note: T[...]

94 Advanced Operations 6.6 W avelength Scan mode – recordin g of absorbance and transmittance spectrums In the wavelength scan mode, the absor bance of the light in a solution over a defined wavelength spectrum is measured. The measurement result s can be displayed as a curve, as percent transmitta nce (%T) or as absorbance (Abs). The collected d[...]

95 Advanced Operations λ Setting wavelength 1. Press the λ key in the Options menu to select the wavelength range and the wavelen gth step. 2. Press the upper lef t key to open the numer ic keypad and select the minimum wave length. Press OK to confirm. 3. Press the upper right ke y to open the numeric keyp ad and select the maximum wavelength. P[...]

96 Advanced Operations 5. According to the selected wavelength range, select the interval from the actively displayed wa velength steps. In summery maximally 780 measuring step s can be accomplished during a Scan. Note: The maximum wavele ngth adjusts aut omatically if the difference between the maximum and minimum wavelength is not a multiple of t[...]

97 Advanced Operations 4. Press Return to return to the scan mode. Note: The Integral is shown instead of the date on the disp lay . Note: For the next scan measurement the setting for the Integral will be On . Scale & units 1. Press Scale & Unit s . 2. Highlight the required unit s ( Abs or %T ). 3. Highlight Auto or Manual scalin g on the[...]

98 Advanced Operations 3. Press Zero . ”Zeroing....“ appears below the g raph as the baseline scan begins. 4. Insert the prepared sample cuvett e/cell into the cuv ette/sample cell holder . 5. Press Read . Below the graph ”Reading...“ appears an d a graph of the absorbance or transmittance values at the scanned wavelengths is displayed cont[...]

99 Advanced Operations 6.6.3 Work with reference scans There are two options to wo rk with Reference Scan : Option 1: 1. Press Reference: Off in the Options menu to select another scan to display on the same sc reen with the current scan. Highlight the required scan number and press Highlight Reference . Note: After selecting a reference scan the R[...]

100 Advanced Operations Option 2: 1. Insert the blank cuvette/cell into the cuvette/sample cell holder . Press Zero . 2. Insert the sample cuve tte/cell into the cuvette/sample cell holder . Press Read . • The newly plotted wa velength scan curves are sh own in black. • The absorbance o r transmittance va lue and the associate d wavelength are [...]

101 Advanced Operations 6.7 T ime course of ab sorbance/transmitt ance The T ime Course Mode is used to collect dat a in either absorbance or transmitt ance for a user-specified length of ti me. After th e data are collected, they can be displayed in eithe r graphic or tabular format. 6.7.1 Time course setup p arameters Press Ti me Course mode in t[...]

102 Advanced Operations Time & interval: 1. Press Time & Interval in the Options menu. 2. Input the total time an d the reading time and press OK to confirm. Note: In total 500 measuring steps are possible. T o select a total time and a time interval that would cause this number of measurements to be exceeded, the time interval is defined a[...]

103 Advanced Operations • Select Mark to mark the next data po int collected. This mark is not used by the instrument, but is available for the user and may indicate a significant even t, such as the additio n of a sample or other reagent. Th e mark is also shown in the table. • Select Sto p to stop taking sample readings. 6.7.3 Analysis of tim[...]

104 Advanced Operations 6.7.3.1 Navigation of a time scan or a time scan analysis After a time sca n has been completed, the time and the absorbance/transmitt ance dat a are displayed as a curve. Where the cursor is positioned on the curve, the elap sed time up to this point and the corresponding abso rbance are highlighted. T able 17 Naviga ting t[...]

105 Advanced Operations 6.8 System checks 1. Press System Checks in the Main Menu. The System Checks menu contain s instrument information an d various performance te sts. 6.8.1 Instrument information 1. Press Instrument Information in the System Checks menu. 2. The model, s erial numbe r and software versio n are displa yed.[...]

106 Advanced Operations 6.8.2 Upgrade of the instrument sof tware T o obtain the sof tware for the update from the Intern et at www .hach.com : 1. Go to http://www.hach.com . 2. On the DR 5000 product page, click Lab System Sof tware / Sof tware Up date Downloads u nder the heading Downloads . 3. Locate the appropriate download an d follow the prom[...]

107 Advanced Operations 6.8.3.1 W avelength check The W avelength Check test is used to check wavele ngth accuracy at 656.1 and 486.0 nm and to check bandwidth at 656.1 nm, only . 1. Press W avelength Check in the Optical Checks menu. 2. Press λ to select the wavelength. 3. Make a choice and co nfirm with OK . 4. Press Star t . Note: During the wa[...]

108 Advanced Operations 5. The result is displayed. 6. Press Cancel to return to Optical Checks. 6.8.3.2 Noise check The Noise Check test is used to test the photometric noise in the instrument. This test can be used to test noi se at any wavelength and at an absorbance level determined by a specific sample. 1. Press Noise Check in the Optical Chec[...]

109 Advanced Operations 5. Insert sample cuvett e/cell into the cell compartment. Close the cell compartment. 6. Press Start . 7. The result will be displayed. Thir ty readings ar e averaged for the blank. The ”A verage“ and ”S td Deviation“ are calculated from 100 consecutive absorbanc e re adings (Noise, Sample, Reference) . Note: Failing[...]

11 0 Advanced Operations 6.8.3.3 Stra y light check The S tray Light t est is used t o measure the stray lig ht in the instrum ent at 220.0 nm an d 340.0 nm. 1. Press Str a y Li g ht C he c k in the Optical Checks menu. 2. Press λ to select the wavelength. 3. Make a choice and confirm with OK . 4. Press Zero . 5. Insert the sample cuve tte/cell or[...]

111 Advanced Operations 6. The ”Average“ an d ”S td Deviation“ are ca lculated from 100 successive abso rbance m easurem ents. The r esult is displayed and is to be comp ared with the nominal/stan dard data (given in the quality control certificate) of the sample cell/test filter . Note: Failings and passings will be defined by the user. 7.[...]

11 2 Advanced Operations 7. The result is displayed and is to be comp ared with the nominal/stand ard data of the test filter . If a specific sample is used, the measured re sults must be evaluated ag ainst user-define d requiremen ts. 8. Press Cancel to return to Optical Checks. 6.8.3.5 Drif t check The Drift Check test is used to test the stabili[...]

11 3 Advanced Operations 5. The Drif t Check runs 1 hour . This test take s a reading every minute for on e hour . Every 15 minutes, linear regr ession is used to calculate the slope (rate of ch ange) for the previou s 15 minute interval. 6. The last reading and slope for each 15 minute inter val are displayed. At the end of the hour , the overall [...]

11 4 Advanced Operations 3. Press Edit . An automatic menu guidance queries values (filte rs, wavelength, nominal values and to lerances) given in the quality control certificate, to the following specifications: • S tray Light • Photometrical accuracy • W avelength accuracy 4. Press OK when all values are entered and the overview is displaye[...]

11 5 Advanced Operations 8. Insert the diff erent filter in the given order on e after the other with the filter glass facing the front of the instru ment. Press Next after inserting a filter and clo sing the cell compartment After the la st measurement the result s are displayed. 9. Press PC & Printer icon to send the da ta to a USB memory sti[...]

11 6 Advanced Operations 6.8.6 Factory service The Factory Se rvice menu is passw o rd protected. This menu is not intended for customer use. 6.8.7 Service time In order to ensure a re gular inspec tion, an auto matic memory reference for the service times can be entered. Af ter switching the instrument on this memory refere nce will be activated a[...]

11 7 Advanced Operations If the next service is du e, the message " Next service is due! " is displayed afte r switching on the instrument. 6. Press OK to return to the Main Menu. Contact the man ufacturer o r distributo r to arrang e an appoin tment for the next service. 6.8.8 Instrument Backup Before the next servic e date the Ins trume[...]

11 8 Advanced Operations Note: If the Backup was already stored b efore, the message "Data already exists. Overwrite?" is displayed. Press OK to overwrite the data. If the file was stored the me ssage Instrument Backup is store d to USB stick will be displayed. 4. Press OK to return to the System Checks menu. Restore backup dat a: Importa[...]

11 9 Advanced Operations Note: If the USB stick is not connected, the message "Please insert USB Memory" is displayed. Connect a USB stick, in order to store the data. Press OK to confirm and press Restore again. 4. Press OK to confirm a fter the me ssag e “Instrument Backup from S/N XXXXXXX. Restore?“ is displayed. 5. After the b ack[...]

120 Advanced Operations[...]

121 Section 7 Maintenan ce CAUTION Potential Chemical, Biologic al Eye and Skin Hazards. Only qualified personnel sho uld conduct the tasks des cribed in this section of the manual. Important Note: Remove any cuvettes/cells that are still in the instrument and dispose of them or their contents using an approve d disposal method. 7.1 Cleaning requir[...]

122 Maintenance 7.2 Lamp replacement CAUTION The UV lamp generates UV light. Do not look directly at an operating lamp without wearing UV pr otective eye glasses. The Lamp comp artment is on the lef t side behind the display and is provided with ventilation on t he back side. Th e tungsten and deuterium (UV) lamp are inst alled in the lamp comp art[...]

123 Maintenance 12. Switch the instrument on. 13. Reset the Lamp History , see section 6.8.5 on page 115 . 7.2.2 Deuterium lamp (UV) replacement 1. Switch the instrument off. 2. Unplug the power cord. DANGER The instrument mu st remain switched of f until the change of lamp has been completed. WA R N I N G Burn Hazard. Wa it until the lamp cools do[...]

124 Maintenance 7.3 Fuse replacement DANGER Certain electrical circuits with in this equipment are protected by fuses against over- current conditions. For continued protection against a risk of fire, replace fuses only with th e same type and rating specified. DANGER Failed fu ses are gene rally an in dication of a prob lem with the equipment. If [...]

125 Maintenance 3. Pull out the plastic base over the power cable socket. 4. Remove the defective fuse, see Figure 1 on page 14 . 5. Insert new fuse (T 2 A H; 250 V) 6. Replace the plastic base. 7. Plug in the power cord. 8. Switch the instrument on. 7.4 Filter p ad maintenance T o determine when the filter mat needs to be replaced, inspect the fil[...]

126 Maintenance Figure 9 Base of DR 5000 with filter grid 1 Filter grid 3 Phillips screw 2 Filter pad Figure 10 Change of filter p ad 1 Filter pad[...]

127 Section 8 T roubleshooting Problem/Dis play screen Likely Cause Action Absorbance > 3.5! The measured abso rbance exceeds 3.5 Dilute the sample and repeat the measurement Concentratio n too high! Calculated concentration is higher than 999999 Dilute the sample and repeat the measurement Error Barcode controlnum ber? Please update program dat[...]

128 T roubleshooting[...]

129 Section 9 Replacement Part s Description Cat.-N o. Sipper Module DR 5000 LZV485 Peltier temperature cont roll ed Module DR 5000 LZV513 Pour-Thru Modu le DR 5000 LZV479 Multi-Cell Holder DR 5000 A23618 Carousel Holder (Sample changer) A23620 USB-Keyboard (keyboard layou t: US) LZV582 USB-Barcode Scanner (handset scanne r for entering sample IDs)[...]

130 Replacement Part s[...]

131 Section 10 Cont act Information HACH Comp any World Headquarters P .O. Box 389 Loveland, Colorado 80539-0389 U.S.A. T el (800) 227-HACH (800) -227-4224 (U.S.A. only) Fax (970) 669-2932 orders@hach.com www .hach.com Repair Service in the United St ates: HACH Company Ames Service 100 Dayton Avenue Ames, Iowa 50010 T el (800) 227-4224 (U.S.A. only[...]

132 Cont act Information[...]

133 Section 1 1 Limited W arranty Hach Company warrant s its product s to the original pu rchaser against any defect s that are due to faulty material or workmanship for a period of one ye ar fr om date of shipment unless otherwise noted in the product manual. In the event that a defect is d iscovered during the warranty period, Hach Compan y agree[...]

134 Limited W arranty[...]

135 Index A Absorbance Check ......... ................ ................ ........ 1 1 1 Absorbance Formula ................... ................ ...... 90 , 91 Alphanumeric keyp ad ............... ............. ................ .. 21 Audio signals ..................... ................ ................. ..... 25 B Barcode Prog rams .................[...]

136 T Time & Interval ................... ................ ............ 101 , 102 Time Course .......... 22 , 38 , 39 , 40 , 41 , 101 , 103 , 104 Time Course Scan Reading ............... ................ .... 102 Timer .................. ................ 25 , 43 , 53 , 54 , 56 , 87 , 90 Touch Scree n ................. ................ .........[...]