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Roper 4411-0070 manuel d'utilisation

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  • Page 1

    4411-0070 Version 2.A September 6, 2001 [...]

  • Page 2

    Copyrig ht 2001 Roper Scientific, Inc. 3660 Quakerbridge Rd Trenton, NJ 08619 TEL: 609-587- 9797 FAX: 609-587- 1970 All rights reserv ed. No part of this publication m ay be reproduced by any means w ithout the written permission of Roper Scientific, I nc. Printed in the United States of Am erica. SpectroPro is a registered tradem ark of A cton Res[...]

  • Page 3

    Table of Contents Introduction .......................................................................................................... 7 Manual Overview ................................................................................................................ 7 Safety Related Sym bols Used I n This Manual ..................................[...]

  • Page 4

    iv NTE/CCD Detector Manual Version 2.A Chapter 5 Microscopy A pplications ................................................................. 27 Introduction ................................................................................................................... .... 27 Mounting the Detector on the Microscope ..............................[...]

  • Page 5

    Table of Contents v Figures Figure 1. NTE/CCD Detectors and Cameras .................................................................... 9 Figure 2. NTE Detector Rear Panel ................................................................................. 11 Figure 3. Annotated Spectrograph Drawing ...................................................[...]

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    vi NTE/CCD Detector Manual Version 2.A This page intentionally left blank.[...]

  • Page 7

    Introduction Manual Overview This manual prov ides the user with all the information needed to install a NTE/CCD Detector and place it in operation. Topics covered include a detailed description of the NTE Detector, installation, microscopy applications, cleaning , specifications and more. Chapter 1 , General Informat i on provides an overv i ew of[...]

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    8 NTE/CCD Detector Manual Version 2.A This page intentionally left blank.[...]

  • Page 9

    Chapter 1 General Information Figure 1. NT E/CCD Det ectors and Camer as Descriptio n The NTE/CCD air-cooled detector or cam era is ideally suited for m edium light lev el applications. State-of- the-art CCD array s are available for the NTE/CCD that enable outstanding perform ance in a wide range of applications for spectroscopy, biological imag i[...]

  • Page 10

    10 NTE/CCD Detector Manual Version 2.A The NTE/CCD detector is available in both C-m ount and F-mount config urations. See Chapter 5 for detailed information on microscopy.[...]

  • Page 11

    Chapter 2 Detector Setup This chapter covers the setup procedures for both im aging and spectroscopic applications. General Instru ctions The following item s are applicable to both im aging and spectroscopic system s. Connecting the D etector Each detector is supplied with a cable to connect to the controller. Make sure that the controller is off,[...]

  • Page 12

    12 NTE/CCD Detector Manual Version 2.A suitable Gain setting. I n situations where the A/D rang e exceeds that of the array, it will generally be better to set the Gain to HI so that the signal can be spread over as m uch of the A/D rang e as possible. This is a particularly im portant consideration in absorbance measurem ents. Users who consistent[...]

  • Page 13

    Chapter 2 Detector Setup 13 Square-form at CCDs can also be used for spectroscopy, although they are m ore often used in imag ing applications. For square form at CCDs (e.g. 512 × 512 or 1300 × 1340), the user may orient the CCD to achieve binning along either direction of the CCD. Binning along the rows (perpendicular mode) m inimizes cross- tal[...]

  • Page 14

    14 NTE/CCD Detector Manual Version 2.A Mounting Di rect i ons 1. Mount the flange to the detector using the two half-ring s and the screws provided. Note that the tapered side of each half- ring faces the adapter (Figure 5). 2. Next, thread the 10-32 hex screws halfway into three of the six tapped holes in the spectrograph’s exit plane. 3. Positi[...]

  • Page 15

    Chapter 2 Detector Setup 15 Second Shutter Type Mount i ng Di rections The second shutter mount, used with all A cton Research spectrographs, requires no disassem bly. Mount it to the spectrograph as shown in Figure 7. Adapter Spectrometer Figure 7. Entrance Slit Shutter for Acton Spectrogr aphs The shutter cable should be connected to the Shutter [...]

  • Page 16

    16 NTE/CCD Detector Manual Version 2.A To Mount the Lens on t he Det ect or: 1. Locate the large indicator dot on the side of the lens. 2. Note the corresponding dot on the front side of the adapter. 3. Line up the dots and slide the lens into the adapter. 4. Turn the lens counterclockwise until a click is audible. The lens is now locked in place. [...]

  • Page 17

    Chapter 3 Cooling the Detector Introduct ion The NTE/CCD detector is desig ned for cooled operation. A four- stage Peltier effect thermoelectric cooler, driv en by closed-loop proportional- control circuitry, cools the CCD. A thermal sensing diode attached to the cooling block of the detector monitors its temperature. A n internal fan draws air thr[...]

  • Page 18

    18 NTE/CCD Detector Manual Version 2.A 4. On the top side of the Temp knob is a rectang ular window that denotes hundreds of degrees C. Each com plete turn of the knob is - 100°C. Around the mov eable part of the knob are num bers from 0 to 99, in increments of 2. Turn the k nob until the correct value (0) appears in the hundreds’ box. 5. Then t[...]

  • Page 19

    Chapter 3 Cooling the D etector 19 Detector loses Temperature Lock The internal temperature of the detector is too hig h. This mig ht occur if the operating environm ent is particularly warm or if you are attempting to operate at a temp erature colder than the specified limit. I f this happens, an internal thermal overload switch w ill disable the [...]

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    20 NTE/CCD Detector Manual Version 2.A This page intentionally left blank.[...]

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    Chapter 4 Focusing Introduct ion Detectors for either im aging or spectroscopic applications must be focused for m aximum resolution. Im aging applications require adjustment of both the lens and the lens adapter. Spectroscopic applications demand both focusing and alignment of the detector to the spectrograph. One of the limitations of scientific [...]

  • Page 22

    22 NTE/CCD Detector Manual Version 2.A fluorescent overhead lamps have good calibration lines as well. I f there are no “line” sources available, it is possible to use a broad band source, such as tungsten, for the alig nment. I f this is the case, use a wavelength setting of 0.0nm for alignm ent purposes. 2. With the Model SP300i properly conn[...]

  • Page 23

    Chapter 4 Focusing 23 Throughput: The throughput of a lens is determined by its aperture, which can ordinarily be set to a num ber of different values, or f/ stops. The higher the number the sm aller the aperture and the lower the throughput. Depth of field considerations mak e it imperative that focusing be done at maxim um aperture (sma llest f/)[...]

  • Page 24

    24 NTE/CCD Detector Manual Version 2.A Imaging Field of View When used for two-dim ensional i maging applicat ions, PI CCD detectors c losely imitate a standard 35 mm detector. Sinc e the CCD is not the same size as the fil m plane of a 35 mm detector, the field of view at a g iven distance is som ewhat different. Princeton Instruments, Inc D O B S[...]

  • Page 25

    Chapter 4 Focusing 25 Remount the lens to the adapter and set the lens focus adjustment to the target distance. 4. Power up the system and, using the application software, select FreeRun , and Safe mode (Asynchronous). 5. Choose a fast exposure (0.1 s) and begin data collection by selecting Focus (WinView/32). 6. Note the im age on the com puter mo[...]

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    26 NTE/CCD Detector Manual Version 2.A This page intentionally left blank.[...]

  • Page 27

    Chapter 5 Microscopy Applications Introduct ion This chapter discusses the setup and optimiz ation of your dig ital imag ing sy stem as applied to microscopy . Since scientific grade cooled CC D im aging system s are usually em ployed for low light level m i croscopy, the m aj or goal is to m aximiz e the light throug hput to the detector. I n orde[...]

  • Page 28

    28 NTE/CCD Detector Manual Version 2.A Mounting the De tec tor on the Micros c ope The detector is connected to the microscope v ia a standard type m ount coupled to a microscope specific adapter piece. There are two basic detector- mounting desig ns, the C-m ount (standard) and the F-mount (optional). The C- mount employ s a standard size thread t[...]

  • Page 29

    Chapter 5 Microscopy Applications 29 Microscope Type Diagnostic Instruments Bottom Clamp Type Leica DMR L-clam p Leitz All ty p es NLW-clamp Nikon Optipho t, Diap hot, Eclipse O-clamp Oly mpus BH -2, B- MAX, IMT-2 V-cl am p Zeis s Ax ioscope, A xioplan , Ax ioplan 2, A xiophot Z-clam p Zeiss Axiovert ZN-clamp Table 1. Bo tto m Clamps for Different [...]

  • Page 30

    30 NTE/CCD Detector Manual Version 2.A NLW O V L ZN Z 1X HRP 100-NIK Figure 11. Diagnostic Instr uments Bottom Clamps for Different Microscopes 1X HRP 100-NIK "L" bottom clamp Figure 12. Bottom C lamp Secured to Relay L ens[...]

  • Page 31

    Chapter 5 Microscopy Applications 31 Note: T he detector lens mount itself also has a focus adjustm ent. Although it is unlik ely that you would ev er need to use this adjustment in operation with a m icroscope (it is preferable that you use the relay -lens focus adjustm ent), it could be used if necessary. The procedure for using the adjustment is[...]

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    32 NTE/CCD Detector Manual Version 2.A This page intentionally left blank.[...]

  • Page 33

    Chapter 6 Operation Introduct ion Once the NTE/CCD Detector has been installed and its optics adjusted as explained in the preceding chapters, operation of the detector is basically straightforward. I n most applications you sim ply establish optimum perform ance using the Focus m ode (WinView/32 or WinSpec/32) and then do actual data acquisition i[...]

  • Page 34

    34 NTE/CCD Detector Manual Version 2.A If y ou observe a sudden chang e in the baseline signal y ou may hav e excessive hum idity in the vacuum enclosure of the detector. T urn off the system and have the detector repumped before resum ing normal operation. Contact the factory Technical Support D ept. for infor m ation on how to refresh the vacuu m[...]

  • Page 35

    Chapter 6 Operation 35 Note that a cam era having the 35 mm shutter can only be used with an ST- 133 equipped with the 70 V shutter option. An ST-133 that has the 70 V shutter drive option can be identified by the 70 V OPT label on its rear panel as shown in Figure 14. Note that there is a Shutter Setting push switch that sets the shutter drive v o[...]

  • Page 36

    36 NTE/CCD Detector Manual Version 2.A Shutter Overheating The 25 mm remote-m ounted shutter for spectroscopy has a built-in therm al interlock to prevent ov erloading of its coil. I f run at a high repetition rate, the shutter m ay heat enough to trig ger the interlock , disabling the shutter. If y our shutter suddenly stops running , stop the exp[...]

  • Page 37

    Chapter 7 Cleaning Turn off all power to the equipment and secure all cov ers before cleaning the units. Otherwise, damag e to the equipment or injury to you could occur. Controller an d Camera Although there is no periodic m aintenance that must be performed on the NTE/CDD Detector, users are advised to w ipe it down with a clean dam p cloth from [...]

  • Page 38

    38 NTE/CCD Detector Manual Version 2.A This page intentionally left blank.[...]

  • Page 39

    Appendix A Specifications Controller Req uirement Requires ST-133 or ST-138 with hig h power modification. Standard ST-133 or ST-138 Controllers cannot meet the NTE’s power requirem ents. CCD Arrays Note: T he following list m ay not be current. Contact the factory for up- to-date information on av ailable chips and chip performance specification[...]

  • Page 40

    40 NTE/CCD Detector Manual Version 2.A Power Maximum internal heat di ssi pation in w at t s: 90 Cooling The internal fan circulates cooling air over the heat exchang er. Openings in the side of the housing prov ide the necessary circulation access. Fan capacity at full power is 30 cfm. A ttainable lock tem perature will vary depending on array siz[...]

  • Page 41

    Appendix B Outline Drawings Note : Dimensions are in inches. COOLING AIR OUTLET TYPICAL BOTH SIDES COOLING AIR INLET TYPICAL BOTH SIDES 6.92 .593 CCD .000 FLANGE 2.598 ±. 001 CENTERING BOSS .279 WINDOW Figure 15. Spectromet er Mount: Side Vi ew Æ .358 HOLE TO Æ .203 SLO TS FOR #10-32 BOL T Æ 3.600 MOUNTING CIRCLE (3 PLACES) 4.63 4.63 EXTERNAL S[...]

  • Page 42

    42 NTE/CCD Detector Manual Version 2.A Figure 17. F-Mount: Si de View 4.63 F-MOUNT (2 3/8 - 20 THREAD NIKON ADAPTER SHOWN 4.63 Figure 18. F-Mount: Fr ont and Back View[...]

  • Page 43

    Appendix B Outline Drawing s 43 2.11 6.16 7.05 0.720 CCD 0.000 MOUNTING SURF ACE COOLING AIR INLET TYPICAL BOTH SIDES COOLING AIR OUTLET TYPICAL BOTH SIDES OPTIONAL TRIPOD MOUNT KIT (2550-0312) 0.500 2.41 ALLOW 1.5” FOR ELECTRICAL CONNECTION 2.25 Figure 19. C- M ount: Side View C-MOUNT (1.00-32 THREAD) 4.63 4.13 Figure 20. C-M ount: Front and Bac[...]

  • Page 44

    44 NTE/CCD Detector Manual Version 2.A ALLOW 1.5” FOR ELECTRICAL CONNECTION 1.52 2.97 2.25 Figure 21. Fiber Opt ic Coupled: Si de and Bottom Views Figure 22. Fiber-Opt ic Coupled: Fr ont and Back View[...]

  • Page 45

    DECLARATION OF CONFORMITY We , ROPER SCIENTIFIC (PRI NCETON INSTRUMENTS) 3660 QUAKER BRIDGE RO AD T R E N TO N , N J 08619 Declare under our sole re sponsibility, that the product ST-133A 1MHz HIGH POWER CONTROLLER w / NTE CAMERA HEAD, To which this declaration relates , is in conformity with general safety requirement for electrical equipment stan[...]

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    46 NTE/CCD Detector Manual V ers i on 2.A This page intentio nally left blank.[...]

  • Page 47

    47 Warranty & Service Limited Warr anty: Rope r Scie nt ific Ana l ytical Instrume ntation Roper Scienti fic, I nc. (“Roper Scien t ific,” u s,” “we,” “ our”) m akes the fo llowing limited warranties. These lim ited warranties extend to th e orig inal purchaser (“You ”, “you”) only and no o ther purch aser or transfe ree. [...]

  • Page 48

    48 N T E/CCD Detector Manua l V ersion 2.A VersArray ( XP) Vacu um Chambe r Limited Lifetime Warranty Roper Scien tific warrants that the cooling performance of the sy stem w ill meet o ur specifica tions over the lifeti m e of the VersA rray (XP) dete ctor or Rope r Scientif ic w ill, at its sole op tion, repair o r replace any v acuum cham ber co[...]

  • Page 49

    Warranty & S er v ice 49 Software Limite d Warranty Roper Scien tific warran ts a ll of ou r manufac tured so ftware disc s to be fre e from substan tial defe cts in m aterials and / o r workm anship under norm al use fo r a per iod of one (1) y ear from shipm ent. Roper Scie ntific do es not warrant that the fu nction of the software w i ll m [...]

  • Page 50

    50 N T E/CCD Detector Manua l V ersion 2.A 7. Warranti es exten d only to defec ts in m aterials o r work manship as limited above and do not e xtend to any product or part whic h has: • been lost or discarded by you; • been dam aged as a result of m isuse, improper insta llation, faulty or inadequate m aintenance or failure t o follow ins truc[...]

  • Page 51

    Index Accessories alignment of, 22 Ape rt u re , 1 6 Backgr ound D C level, 3 3 Baseline signal, 33 Binning along colum ns, 13 along r ows, 1 3 Bottom Clamps, 28 table of , 29 Calibration suitable light sources, 21 Cautions baseline signal shift, 34 IR contam ination, 31 scintillator & UV, 33 CCD perpendicul ar m ode operation, 12 square form a[...]

  • Page 52

    5 2 N T E/CCD Detector Manua l V ersion 2.A NTE/CCD connec ting the lens, 1 5 enclosures, 9 setu p, 11 temperatu re range, 17 Optical path, 12 Outline drawings, 41 Overexposu re, 16 Overexpos ure protection , 15 Parfocality, 29 Peltier effect, 17 Photodam age, 27 Power suppl y switches, 11 Quantum Efficiency (QE), 27 Readout pat tern subtract ion o[...]