Hach Portable Spectrophotometer DR 2800 manuale d’uso

DOC022.53.00720 DR 2800 USER MANUAL January 2008, Edition 2 © HACH Company , 2007–2008. All rights reserved. Printed in Germany .[...]

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3 T able of Content s Section 1 Specification s ............ ................ ................ ................ ............. ................ ................. ................ .... .......... 7 Section 2 General Information ..... ................ ................ ................. ................ ................ ................ ...............[...]

4 T able of Content s 5.3 Store, recall, send and delete data .... ....... .......... ................ ................ ................ ................ .. ..................... ...... 37 5.3.1 The data log .............. ................ ................. ................ ................ ................ ............. ............. .............[...]

5 T able of Content s 6.2 Favorite Programs . ................ ................ ................ ................ ................. ................ ....... ............ ................ ...... 81 6.2.1 Recall a favorite program ........ ................ ................ ................ ................ ................ ....... ...................[...]

6 T able of Content s[...]

7 Section 1 S pecifications S pecifications are subjec t to change without notice. Performance Specifications Operating Mode T ransmittance (%), Absorban ce and Concentration Source Lamp Gas-filled Tungsten (visible) W avelength Rang e 340–900 nm W avelength Accur acy ± 1.5 nm W avelength Reproducibility < 0.1 nm W avelength Resolutio n 1 nm [...]

8 Specifications[...]

9 Section 2 General Information 2.1 Safety information Please read this entire manual befo re unpacking, setting up o r operating this equipment. Pay attention to all danger , warning and caution statem ents. Failure to do so cou ld result in serious injury to the operator or damage to th e equipment. Make sure that the protection prov ided by this[...]

10 General Information Permissible Exposure value. Therefore, for Class 1 LASERS the output power is below the level at which it is believed eye damage will occur . Exposure to the beam of a Class 1 LASER will not result in eye injury . Class 1 LASERS may therefore be considered safe. However , Class 1 LASER products may contain LASER systems of a [...]

11 Section 3 Inst allation WA R N I N G Electrical and F ire Hazards. Use only the pr ovided power supply . Only qualified per sonnel should conduct the tasks described in this sect ion of the manual. 3.1 Unp ack the instrument The DR 2800 S pectrophotometer comes packaged with the following items: • DR 2800 spectrophotom eter • Plug-in power s[...]

12 Inst allation 3.3 Power connections Insta ll the correct adapter plug on the supplied external power supply ( Figure 1 ) by sliding the adapter on until it "clicks" into position. Correctly mounted, bo th housing of power supp ly and plug are in line. Plug the external power supply cord into th e connector on the back pane l of the ins[...]

13 Inst allation 3.4 Connection The DR 2800 has two USB interfaces as a stan dard feature, located on the back of the instrument ( Figure 2 ). The USB T ype A interface is used for communications with a printer , USB memory stick, barcode scanner , or keyboard. A USB memory stic k is used to up date instrument software. The USB T ype B interface is[...]

14 Inst allation 3.5 Cell comp artment s , Cell adapters, Light Shield and Protective Cover 3.5.1 Cell comp artment s and adapters The DR 2800 has two cell compar tments ( Figure 3 ). Only one cuvette/sample cell type at a time can be used for a measur ement. Cell comp artment #1 • 13-mm and 16-mm rou nd cuvettes/vials Note: Cell compartme nt #1 [...]

15 Inst allation 3.5.2 Inst allation of the cuvette/sample cell adapters 1. Open the cell compartmen t. 2. Select the correct adapter for the cuvette/sample cell type. 3. Insert the adapter so the arro w on top of the adapter point s to the left ( Figure 5 on page 16 ) and th e orientation t ab fit s the groove in the comp artment openin g. The cuv[...]

16 Inst allation 3.5.3 Use of the light shield for measurements The light shield ( Figure 6 on page 17 ) prevent s light interference when using 13 mm and 16 mm vial test s and must be in place before meas urements can be taken in cell compartmen t #1. The light shield is required only when using 13 mm or 16 mm vial tests. Figure 5 Installation of [...]

17 Inst allation The DR 2800 is shipped with the light shield installed. Remove the light shield before using cell comp artment #2. The light shield can be stored in the Protective Cove r ( Figure 8 o n page 18 ) Inst allation of the light shield 1. Open the cell compartmen t. 2. Insert the light shield so the ar row on the light shield points to t[...]

18 Inst allation 3.5.4 Protective Cover Figure 8 Protective Cover 1 Protective Cover 3 Protective Cover with inse rted cuvette/ sample cell adapter A in position A. 2 Protective Cover (inside view) The inside of the Protective Cover is intended to house the cuvette/sample cell a dapters. The recesses for holding the cuvette/sample cell adapters are[...]

19 Inst allation 3.6 Mobile use of the DR 2800 for field analysis If the DR 2800 spectrophotometer is used to carry out field analyzes, it may be necessary to t ake a number of measures to compensate for the fluctuating ambient lig ht conditions. Y ou sh ould take the measures described be low if the following warning is displayed af ter you take a[...]

20 Inst allation 3.6.1 Position the Protective Cover 1. After you have inserted the zero solution cell or the sample cell, position the Protective Cover over the open cell co mpartment. The lettering "Protective Cover" must be reada ble from the display side of the instrument. 2. Press the Protective Cover lightly into the correct positio[...]

21 Inst allation 3.7 Beam p ath Figure 10 shows the be am path of the DR 2800: Figure 10 Beam path 1 T ungsten lamp 8 S plitter mirror 2 Entrance slit 9 Reference-e lement 3 Heat-protection glass 10 Cell compartment #2 4 Grating 11 Lens 5 Exit slit 12 Measurement element 6 Lens 13 Cell compartment #1 7 Filter wheel 14 LED[...]

22 Inst allation[...]

23 Section 4 S t a rt Up 4.1 Power the ins trument on and off 1. Plug in to the p ower supply (laborator y analysis) or inser t the battery (field analysis). 2. The push-button switch on the back of the instrument switches the instrument on (press for ab out 1 second) and of f (press for about 3 to 5 seconds). An acoustic signal confirms that the i[...]

24 Star t U p If the instrument detect s any deviation re lative to the last calibration, a system check has to be carried out. 4. Remove the cuvette from the cell compartment and close the lid. 5. Then press Start . The system check is carried out (duration approx. 6 minutes). Note: Further error messages du ring self check, see Section 8 on page [...]

25 Section 5 S t andard Operations 5.1 Overview 5.1.1 Tip s for the use of the touch screen The entire screen is to uch-activate d. T o make a selection, pr ess the screen with a fingernail, fingertip, pencil eraser or a stylus. Do not press the screen with a sharp object, such as the tip of a ball point pen. • Do not place anything on top of the[...]

26 St an dard Operations 5.1.3 Main Menu A variety of modes may be selected fr om the ”Main Menu“. The following table br iefly describes each menu option. T able 2 ”Main Menu“ Options DR 2800 Option Function Stored Pro grams / Barcode Programs (HACH LANGE Programs) S tored programs are pre-programmed methods t hat make use of HACH reagents[...]

27 St andard Operations 5.2 Instrument Setup mode 1. Select Instrument Setup in the ”Main Menu“. A selection of functions appears in order to configure the functions of the instrument. 5.2.1 Operator ID Use this option to enter up to 30 set s of operator initials (up to 5 characters each) into the instrument. T his feature help s record which o[...]

28 St an dard Operations 5.2.2 Sample ID Use this option to ente r up to 100 Sample Identif ication tags (up to 13 character s each) into th e instru ment. Sample IDs can be used to specify the sample location or ot her sample specif ic information. 1. Press Sample ID in the Instrument Setup. 2. Press New to enter a new Sample I D. 3. Use the alpha[...]

29 St andard Operations 5.2.3 Date and time 1. Press Date & Time in the Instrument Setup. 2. The date and time are subdivided over a number of fields. Press the appropriate field and use the arrow keys to change the value. 3. Press OK to confirm. The inst rument will return to Instrument Setup. 5.2.4 Display and sound preferences 1. Press Displ[...]

30 St an dard Operations 2. Select one of the options under S t andby mode to se t the length of the idle period th at can elapse before the instr ument switches to the S tandby mode whe n running on b attery power . Note: In standby mode, the backlighting of the screen is switched off. T ouchin g the display caus es the lighting to switch on again[...]

31 St andard Operations 5.2.6 PC and printer The instrument is provided with 2 USB interfaces, which are located on the back of the instrument (see Figure 2 on page 13 ). These interfaces c an be used for expor ting data and graphics to a pr inter , updating dat a and for dat a communication to a personal computer . These interfaces can be used for[...]

32 St an dard Operations Printer Setup: • Resolution: Print quality • Paper: Paper size Note: If an optional Thermal Pri nter is connected, the function "Auto Send" on/off is available. 4. Select Auto-Send: On to send all measured dat a automatically to the Thermal printer . Note: The option Auto-Send is not available for any othe r p[...]

33 St andard Operations 7. Press Paper to select the paper size. Select between • Letter • Legal • Executive • A4 8. Press OK to confirm. Note: Press OK again to return to the PC & Printer menu. 5.2.6.2 Print dat a 1. Press Recall Dat a in the Main Menu. 2. Select the data sou rce, where the data to be printed are stored. 3. A list is d[...]

34 St an dard Operations 4. Select Auto-Send: On to send all measured dat a automatically to the PC. Note: If Auto-Send: Off is selected, the PC & Printer icon must be pressed, in order to send data to the PC. Note: The remote funct ion is onl y fo r monitoring the data transfer . 5.2.7 Password The Password menu cont ains a variety of security[...]

35 St andard Operations 4. Press Security List to lock various functions for unauthorized users. 5. Highlight the d esired funct ions to cont rol. 6. Confirm the Security List wi th OK to return to the Password menu. 7. Press On to highlight the new settings of the Security List. 8. Enter the n ew Password again to conf irm. 9. Press OK to return t[...]

36 St an dard Operations 3. Press Off to deactivate the settings of the Security List. 4. Press OK to return to Instrument Setup. Note: Use this function to dele te the former Password or to enter a new one.[...]

37 St andard Operations 5.3 S tore, recall, send and delete dat a 5.3.1 The dat a log The Data Log will store up to 500 readings taken in the modes: S tored Programs, Barcode Programs, User Programs, Favorite Programs, Single W avelength and Multi W avelength. A complete record of the analysis is stored, including the Date, T ime, Results, Sample I[...]

38 St an dard Operations 4. The function Filter Settings is used to search for specific items. 5. Highlight On to turn on the filt ers to select data by • Sample ID • Operator ID • St a r t D a t e • Parameter or any combination of the four . 6. Press OK to confirm the se lection. The ch osen items are liste d. 7. Press Vie w De tai ls to g[...]

39 St andard Operations 5.3.1.4 Delete stored dat a from the dat a log 1. Press Recall Dat a in the Main Menu. 2. Press Dat a Log>Options>Delet e . 3. Highlight Single Po int or Filtered data or All dat a and press OK to confirm. Note: The number in parentheses is the total number of data set s assigned to this selection.[...]

40 St an dard Operations 5.3.2 Time Course The instrument can store 20 T ime Course Dat a sets. The dat a can be stored m anually at the user's discre tion after viewing the data. 5.3.2.1 Dat a Storage f rom Time Course 1. Press the Stor e i con in the ”Options“ menu from T ime Course mode after a reading is taken. The S tore Data list ope[...]

41 St andard Operations 2. Press Graph to look at det ails. Note: Press V iew Summary to return to the Recall Data list. 3. Press Ta b l e to look at details. Note: Press V iew Summary to return to the Recall Data list. 5.3.2.3 Send Dat a from T ime Course Option 1: 4. Press Recall Dat a in the ”Main Menu“ and then Tim e Co ur se to send the da[...]

42 St an dard Operations Option 2: 5. Press Time Course and then Options , More... and Send Dat a to send the dat a to a USB me mory stick or to a printer . The files will be automatically send as CSV files (Comma Separa ted V alue) to a file ”TCDat a“ (T ime Co urse Data ). The file name will be formatted as: ”TCY ear_Month_Day_Hour_Minute_S[...]

43 St andard Operations 5.4 S tored Programs The instrument cont ains more than 200 prog rammed procedures. They can be accessed through the S tored Programs menu. 5.4.1 Select a saved t est/method; enter user -specific basic data 1. Press S tored Programs in the Main Menu to view an alphabetical list of stored pr ograms with progra m numbers. The [...]

44 St an dard Operations Store Of f/On With the Sto r e O n settin g, all measurement data are stored automa tically . W ith the St or e Of f setting, no measurement data are stored. % T rans/Conc/A bs To switch to % transmitt ance, co ncentration or absorbance readings Send Dat a icon / Send Data T o send Da ta to a printer , computer or USB memor[...]

45 St andard Operations 5.4.3 Use of pr ogram timers Some procedures do not re quire the use of timers. Other procedures require several tim ers. These timers are pre-program med into each Stor ed P ro g ra m , along with a description of the activity to be performed dur ing the timed period. 1. Press the Time r icon on the display . 2. Press OK to[...]

46 St an dard Operations 5.4.4 Set the dilution factor The Dilution Factor function is used to calculate the original concentration of a sample that has been diluted by a known ratio. For example, dilution factor can bring the analyze d concentration within the test range. 1. Press Options > More… > Dilution Factor . The number entered at t[...]

47 St andard Operations Read a stand ard before setting S tandard Adjust to On. 1. Follow the entire procedure, u sing a known standar d for the sample. 2. After rea ding the concentration, press Options>More>S t andard Adjust . 3. If S tandard Adjust is set to Off , turn it On . The Current Reading will show th e concentration. The box on th[...]

48 St an dard Operations 1. Press Options>More>Chemical Form . 2. Select the Chemical Form. Note: The stoichiometric conve rsion of the measuremen t result is carried out automatically . Note: The selected Chemical Form wi ll appear on the display . T est results will be calculated and displayed in this chemical form. 5.4.6.1 Change of the de[...]

49 St andard Operations 4. Press Options>More>Rea gent Blank . 5. Press On to hig hlight the Reagent Blank function. 6. The concentration shown on the key is the measured value of the reagent blank. T o use this value for more analyses of this paramete r , press OK . 7. T o enter a previously recorded reagent bla nk value, press the key and u[...]

50 St an dard Operations 4. Remove zero solution and insert sample cuvette/cell into the cell compartment. 5. Press Read . The result w ill be displayed. 6. For data storag e, see section 5.3.1 on page 37 . 5.4.9 Add stored programs to the favorite programs list The Favorites men u simplifies test selection by creating a list of the most freque ntl[...]

51 St andard Operations 5.5 Barcode Programs A special barcode reader in cell comp artment #1 automa tically reads the barcode on the 13 mm cu vette/vial as the cuvette/vial completes a single rotation. The instrum ent uses th e barcode identification to automatically se t the correct wavelength for the analysis and calculates the result immediatel[...]

52 St an dard Operations • Otherwise, press Barcode Programs in the Main Menu. and insert the blank or sample cu vette/vial (depending on the working procedure ) in cell compartmen t #1. The measurement is st arted automatically and the results ar e displayed. T o evaluate other cuvette/vial test s and other p arameters, insert the prepared cuvet[...]

53 St andard Operations 5.5.3.1 Change of the default setting of the chem ical form 1. Insert the blank or sample cuvette/cell (depending on the working procedure) into the cell comp artment. 2. In the result display , press Options>More>Chemical Form . 3. A list of available evaluation forms appear . Select the new default setting. 4. Press [...]

54 St an dard Operations 5.5.5 Sample blank T urbidity and color in the sample ma trix can falsify the results of a photometric analysis. The inte rference factors come from the sample or are created by reactions with the reagents. The influence of turbidity and/or color can be eliminated or reduced by taking a sample b lank readin g. In the barcod[...]

55 St andard Operations 5.5.6 Up date/edit barcode tests 5.5.6.1 Manual up date of a barcode test Using the data provided in the barcod e, the instrument automatically set s the measurement wavelength and factors. If a discrepancy is detected between the barcode data and the stored data or a new te st is identified, the instrument r equests an up d[...]

56 St an dard Operations Enter the V ersion 1. Highlight the line containing Ve r s i o n and press Edit . Use the alphanumeric keyp ad to enter the name indicated in the working proced ure. 2. Press OK to confirm. Enter the Me asurement Process For detailed info rmation on entering the m easurem ent process , see section 6.1.2 on page 72 . Check t[...]

57 St andard Operations Enter the V ariables (Factors, W avelength and Coversion Factors) For detaile d information on entering the V ariables, see section 6.1.2 on page 72 . Check the working procedur e in advance to determine wheth er this point needs to be changed . 1. Highlight the Va r i a b l e s line in the data overvie w and press Edit . 2.[...]

58 St an dard Operations 5.5.6.2 Up date an existing barcode test Note: Only in very few cases does the re vision of a test requi re all test specifications to be updated. Option 1: Manual up date of test dat a 1. Insert the light shield in cell compartment #2. 2. The instrument needs to be at the Main Menu or press Barcode Programs in the Main Men[...]

59 St andard Operations 5.5.6.3 Program a new test 1. The instrument needs to be at the Main Men u or press Barcode Programs in the Main Menu. 2. Insert the sample cuvette/vial or blank (dependi ng on the working procedure) into the cell comp artment. 3. After "Program not available", a data overview is displayed, including the specif ica[...]

60 St an dard Operations 6. Press OK to return to the System Checks menu. Note: When the instrument software has be en updated, a prompt to rest art the instrument is displayed.[...]

61 Section 6 Advanced Operations 6.1 User Programs User programs provide the op portunity to complete "made to measure" analysis. The User Progra ms database is em pty when the instrum ent leaves the factory and is used to acco mmodate programs cre ated by users specific need s. Here are a few examp les of entrie s: • Programming of use[...]

62 Advanced Operations Program Numb er: S pecific test number , with whic h the progra m can subseq uently be called up from the selection list in the User Programs menu or the Favorites menu. 2. Use the alphanumeric keyp ad to enter a program number between 950 and 999. The lowest available number a ppears automatically . 3. Press OK . Note: If th[...]

63 Advanced Operations 6.1.1.1 Single wavelength settings If the Single Wavelength m ode is selected, the following parame ters can be defined: Unit s: Select the required unit from the list and press Next . Note: Units of measure not included in this list can be added in the edit program under Program Options , Edit . Select Units , Edit and th en[...]

64 Advanced Operations Chemical form: Enter the chemical formula used in the display to represent the analysis parameter . Use the alphanumeric keyp ad to enter the chemical form and press Next to enter Calibration settings. 6.1.1.2 Multi wavelength settings If the Multi W avelength mode is se lected, the follo wing p aramete rs can be defined: Uni[...]

65 Advanced Operations List of available absorbance for mulas A 1 is the absorbance at waveleng th 1, A 2 is the absorbance at waveleng th 2 and so on K 1 is the factor at wavelength 1, K 2 is the factor at wavelength 2 and so on If a subtraction has to be completed, the factor s can be entered with a minus sign. Wa v e l e n g t h λ x : Press a ?[...]

66 Advanced Operations Chemical form: Enter the chemical formula used in the display to represent the analysis parameter . Use the alphanumeric keyp ad to enter the chemical form and press Next to enter Calibration settings. 6.1.1.3 Calibration settings for single and multi wavelength mo de A method is calibrated by dete rmining the absorbance valu[...]

67 Advanced Operations 2. T o enter the standard concentrations and cor responding absorbance value s in the displayed t able, press the " + " symbol. Use the alphanume ric keypad to enter the values. Press OK and enter th e corresponding absorbance value. Press OK . The entered dat a are displayed in the t able. Repeat the sequence for e[...]

68 Advanced Operations Calibration by r eading st andards 1. Press Read St andards and press Next . 2. T o enter the standard con centrations in the displa yed table, press the " + " symbol. Use the alphanumeric keyp ad to enter the standa rd concentration. Press OK . 3. Press the "+" s ymbol agai n (see arrow ) and ente r the n[...]

69 Advanced Operations 9. Press Force 0 to change the setting from Of f to On . The curve then passes throug h the origin of the coordinate system. Note: This may have an adverse effect on the correlation coefficient (r 2 ). 10. Press Ta b l e to display the table ag ain. 11 . When the t able has been completed and the curve type has been chosen, p[...]

70 Advanced Operations 6.1.1.4 Store a user program The input of the basic data is complete. An overview of the variable program d ata is displayed. 1. T o enter more specifications or change existing ones, highlight the appropriate line and press Edit . 2. Select St or e to save the user program. 3. Press the PC & Printer icon to send the prog[...]

71 Advanced Operations Timer 1 / T imer 2 / Timer 3 / T imer 4: This function can define time interv als for up t o four timers. T imer designations such as Shake, W ait and Swirl can be assigned. 1. Highlight the appropriate line in the overview of the program data an d press Edit . 2. The timers are activated or deact ivated with the check boxes [...]

72 Advanced Operations 6.1.2 Free programming program type Free Programming is an advan ced option for entering original user-devel oped methods. When the Free Programming option is selected, an overview of the specifications of the progra mmed test is displayed. Each inpu t option can be modified to d evelop the user method. Refer to Table 10 for [...]

73 Advanced Operations • Should individual program seq uences be repeate d? The element s of a measuring sequence, such as zero and sample measurement s and the timer(s) (reaction ti mes, waiting times, etc.) are individually defined. 6.1.2.2 Enter a new element of a me asuring sequence Important Note: Each co mponent of the measurement process m[...]

74 Advanced Operations Process T imer key 1. Press the Process T imer key to enter any w aiting, react ion or handling tim es that have t o be taken into acc ount. Use th e alphanumeric keyp ad to enter the time. Press OK and co nfirm the input by pressing OK again. Note: This time is integrate d into the measurement process. Note: The entered meas[...]

75 Advanced Operations Deleting an element of a meas uring sequence Select the appropriate line and press Delete . The element is deleted. Inserting an elemen t of a measurin g sequence Select the line in the mea suring sequence where the insertion is be made and press New . A new element can be entered at the selected position. When the inpu t is [...]

76 Advanced Operations Refer to Table 11 for detailed information on the Edit formula keys. Note: The evaluation formula is built up successively in the disp lay in accordance with the input. Note: The arrow key del etes the most recent ly entered element of the formula. T able 1 1 Edit formula key descriptions Screen Key Description Abs/V ariables[...]

77 Advanced Operations Enter the ne xt calibration formula (C2 or Cn) 1. Highlight C2: Off a nd press Edit . 2. Select C2: Off again and press Edit . The display switches to C2: On . 3. Highlight the next line C2 = to defin e the formula and pr ess Edit . In addition to the keys described in Table 11 , on ly one other function is available: +– ÷[...]

78 Advanced Operations Select Conc. key If an already defined formula, in this case C1, is to be take n into account in the formula for C2, p ress Select conc. Enter the nu mber of th e formula (e.g. 1 for C1 ) and pres s OK to confirm. Cn can now be linked with a mathematical operation. Note: The Cn concentrations that are to be calcu lated are nu[...]

79 Advanced Operations 6.1.3 Select a user program 1. Press User Programs in th e Main Menu to view an alphabetical list of user pr ograms with progra m numbers. The User Programs list will appear . Note: Use the scroll bar to scroll throug h the list quickly . Note: Highlight the selection by pre ssing it or press Select by Number to search for th[...]

80 Advanced Operations 6.1.4.1 Add to Favorites 1. Press Add to Favorites and press OK to confirm. The program is ad ded to the Favorites. 6.1.4.2 Edit 1. Press Edit and pres s OK to confirm. An overview of the specificati ons of the programm ed test is displayed. More in formation about th e input options is pr ovided in the section 6.1.2 on page [...]

81 Advanced Operations 6.2 Favorite Programs The most frequently used tests/methods in the St ored Programs menu and the User Programs menu can also be added to the list of favorites to simp lify their selection. T o add Stored Programs and/or User Programs to the favorites list or the favorite prog rams, see section 6.1.4 on page 79 . 6.2.1 Recall[...]

82 Advanced Operations 6.3 S t andard Addition – monitoring/check ing result s The accuracy of measured values (their correspondence with the actual concen tration of the analyte in the sample) and their precision (corre spondence of the measurement re sults obt ained from several samples containing the same concentration of the test analyte) can[...]

83 Advanced Operations e. Does the te st require t he sample to be at a cer tain temperature? f. W as the pH of the sample in the correct range? g. Is the pipette volume correct? 2. Check the used reagent s by repeating the stan dard addition procedure with fr eshly prepared re agents. If none of these measures reso lves the problem, please cont ac[...]

84 Advanced Operations Peak volume/samp le volume methods 1. Select Stored Progr ams in the main menu. Select the requir ed program. 2. Press Star t . 3. Analyze a sample without added st andard solution in accordance with the instruction s in the Procedures Manual. When the measurement is complete, leave the sample cuvette/cell in the cuvette/samp[...]

85 Advanced Operations 6. Press the key of the value to change it. Use the alphan umeric keypad to change the value. Press OK to confirm. 7. Press the keys to enter the st andard addition volumes. Use the alphanumeric keyp ad to enter the new dat a and press OK . Description of the t able of readings • The first column sh ows the standard a dditi[...]

86 Advanced Operations 11 . After all the standard ad dition solutions have been measured, press Graph . The regressio n line through the standard a ddition data points is displayed. The correlation coef ficient r 2 indicates how c lose the data points are to th e line. If the correlation co efficient = 1, the curve is linear . The concentration sh[...]

87 Advanced Operations 6.4 Single W a velength (absorbance, concentration and tran smitt ance measurement s) The Single W avelength mode can be used in thr ee ways. For sample measurement s at a single wavelength, the instr ument can be programmed to measure the absorba nce, % transmittance or concentration of the analyte . Absorbance me asures the[...]

88 Advanced Operations Concentration factor: 1. Press Concentration Factor: Off in the Options menu. Press On to highlight this feature. 2. Press the ”Factor“ key and use the alphan umeric keypa d to enter the f actor by w hich absorban ce reading s are to be multiplied. Press the ”Unit“ key to select the units for concentration measurement[...]

89 Advanced Operations 6.4.2 T ake single wavel ength measurements 1. Insert the blank cuvette/cell into the cuvette/sam p le cell holder. Press Zero . Note: The Read key is only active after the zero measurement has been completed. 2. Insert the sample cu vette/c ell into the cuvette/sample cell holder. Press Read . 3. For dat a storage, see secti[...]

90 Advanced Operations 6.5 Multi-W avelength mode – measureme nt s at more than one wavelength In the multi-wavelength mode, absorbance values can be measured at up to four wavelengths and the result s can be mathematically processed to obt ain sums, dif ferences an d relationship s. Absorbance measures the amount of light ab sorbed by the sample[...]

91 Advanced Operations λ / Absorbance formula: 1. Press Absorbance For mula . 2. The formula selected in the to p key determines the number of wavelength and coef ficent keys that will appear below . T o change the absorbance formula, pr ess the top key , select a formula from the displayed list and press OK . When a new formula is selected, the n[...]

92 Advanced Operations 3. T o change a wavelength, press one o f the ” λ x:“ keys. Enter the desired wavelength coef ficient into the numeric keypad . Press OK to confirm. 4. T o change a coefficient, press one of the ”K X :“ keys. Enter the desired coef ficient into the numeric keypad. Press OK to confirm. Note: The instrument allows entr[...]

93 Advanced Operations 6.5.2 Complete a measurement in the multi wavelength mode 1. Insert the blank cuvette/cell into the cuvette/sam p le cell holder. Press Zero . Note: The Read key does not be come active until th e zero measurement has been completed. 2. Insert the sample cu vette/c ell into the cuvette/sample cell holder. Press Read . 3. For [...]

94 Advanced Operations 6.6 T ime course of absorbance/transmitt ance The T ime Co urse Mode is used to collect dat a in either absor bance or transmittance for a user-spe cified length of time. After the data are collected, they can be displayed in eith er graphic or tabula r format. 6.6.1 Time course setup p arameters Press Time Cour se mode in th[...]

95 Advanced Operations Time & interval: 1. Press Ti me & Interval in the Options menu. 2. Input the total time and the re ading time and press OK to confirm. Note: In total 500 measuring steps are possible. T o select a to tal time and a time interval that would cause th is number of measurements to be exceeded, the time interva l is define[...]

96 Advanced Operations • Select Mark to mark the next data point collected. This mark is not used by the instrument, but is available fo r the user and may indicate a significa nt event, such as the addition of a sample or other reagent. The mark is also shown in the table. • Select St op to stop taking sample readings. 6.6.3 Analysis of time c[...]

97 Advanced Operations 6.6.3.1 Navigation of a time scan or a ti me scan analysis After a time scan has been comp leted, the time and the absorbance/ transmittance data are displaye d as a curve. Where the cursor is positioned on the curve, the elap sed time up to this point and the corresponding absor bance are highlighted. 6.7 System checks 1. Pr[...]

98 Advanced Operations 6.7.1 Instrument informati on 1. Press Instrument Information in the System Checks menu. 2. The model, serial num ber and software version ar e displayed. 6.7.2 Upgrade of the instrument sof tware T o obtain the sof tware for the update from the Intern et at www .hach.com: 1. Go to http://www.hach.com . 2. On the DR 2800 prod[...]

99 Advanced Operations 6.7.3 Optical checks For each optical check, the measured r esults must be evaluated against user-specific requirement s. The check options do not define tolerances. 1. Press Optical Checks in the System Checks menu. The Optical Checks menu cont ains programs for checking the wavelength accuracy , stray light and photometric [...]

100 Advanced Operations 6.7.3.2 Stra y light check The S tray Light t est is used t o measure the stray lig ht in the instrument at 340 nm. 1. Press Str a y Li g ht C he c k in the Optical Checks menu. 2. Remove any cuvette/sample cell from the cell comp artment. 3. Insert the adapter (A) for 10-mm rect angular cells in the cell compartment #2. Pre[...]

101 Advanced Operations 6.7.3.3 Absorbance check The Absorbance Check test is used to test the photometric accuracy and repeatabilit y of the instrument. This test can be used to test ab sorbance at any wavelength by a specific sample or test filter set (Refer to section 6.7.3 .4 on page 102 ). 1. Press Absorbance Check in the Optical Checks menu. [...]

102 Advanced Operations 6.7.3.4 V erification kit The V erification Kit, LZV537, (see Section 9 on page 119 ) is designed for periodic monitori ng of scattered light, photometric accuracy and th e wavelength accuracy of the spectro photometers. When results exce ed allowable tolerances (given in the quality control certificate), cont act the manufa[...]

103 Advanced Operations 7. Remove any cuvettes/cells from the cell comp artment and press Start . 8. Insert the diff erent filter in the given order on e after the other . Press Next af ter inserting a filter . After the la st measurement the result s are displayed. 9. Press PC & Printer icon to send the data to a USB memory stick, PC or to a p[...]

104 Advanced Operations 6.7.4 Output checks If a printer is connected a test prin ting of the current screen will be printed. 6.7.5 Lamp history The Lamp History menu provides t he amoun t of time that the lamp has been on (Hours). After a lamp is replaced and the Lamp History is reset, th e display of the tot al operating time is reset to 0. 1. Pr[...]

105 Advanced Operations 6.7.7 Service time In order to ensure a regular inspection, an automatic memory reference for the service times can be ente red. After switching the instrument on this me mory reference will be ac tivated and indicated at the appropriate time. 1. Press Service Ti me in the System Checks menu. 2. Select On and then Last Serv [...]

106 Advanced Operations 6.7.8 Instrument Backup Before the next service date the Instrum ent Backup menu offer s the possibility to store all program s, measuring data, Operator ID, Sample ID, passwords and all adjustable data on a USB stick. 1. Press Instrument Backup in the System Checks menu. 2. Connect the USB memory stick ( section 3.4 on page[...]

107 Advanced Operations If the file was stored the message Instrument Backup is stored to USB stick will be displayed. 4. Press OK to return to the System Checks menu. Restore backup dat a: Important Note: All current data will be ov erwritten when restoring the Backup file! 1. Press Instrument Backup in the System Checks menu. 2. Connect the USB m[...]

108 Advanced Operations 4. Press OK to confirm after the message “Inst rument Backup from S/N XXXXXXX. Rest ore?“ is displayed. 5. After the ba ckup start the instrument again.[...]

109 Section 7 Maintenan ce CAUTION Potential Chemical, Biologic al Eye and Skin Hazards. Only qualified personnel sho uld conduct the tasks des cribed in this section of the manual. Important Note: Remove any cuvettes/cells that are still in the instrument and dispose of them or their contents using an approve d disposal method. 7.1 Cleaning requir[...]

11 0 Maintenance 7.2 Insert or change of the battery 1. Remove the cuvette from the cell compartment. 2. Switch the instrument of f. 3. Unplug the power cord. 4. T urn the instr ument over carefully and place it on a soft surface. 5. Use a screwdrive r or a coin to remove the screw from the cover . 6. Remove the cover and place it to one side. CAUT[...]

111 Maintenance 8. Push in the battery so that the contacts of the instrume nt can be plugged into the socket of the batter y . Important Note: T ake care that the battery contacts are always clean. Soiled contacts can result in contact heating and voltage drop, interfering with th e proper operation of the spectropho tometer. 9. Use a screwdriver [...]

11 2 Maintenance • The performance of the battery d ecreases with the temperatur e. 7.2.1.2 Optimal operation of the battery The battery is charg ed automatically when the plu g-in power supply on the back of the instrument is connected and is plugged into a mains socket (100–2 40 V olt/50 –60 Hz). CAUTION Use only the manufactur er's sp[...]

11 3 Maintenance 7.3 Lamp repl acement CAUTION T o avoid a possible electric s hock, disconnect the instrument from the power source be fore servicing the lamp. 1. Remove the cuvette from the cell comp artment. 2. Switch the instrument off. 3. Unplug the power cord. WA R N I N G Burn Hazard. Wa it until the lamp cools down. Contact with t he hot la[...]

11 4 Maintenance W ARNING Burn Hazard. W ait until the lamp cools down. Contac t with the hot lamp can cause burns. 9. Lift the lamp fitting carefully . 10. Put both screws to one side. 11 . Push up on the pre ssure spring. 12. Remove the halogen lamp complete with the plug p anel.[...]

11 5 Maintenance 13. Carefully unplug the halogen lamp from t he plug panel. Important Note: Ho ld the lamp by the fitting only . Avoid to uching the glass, as substances on the ski n can bake onto the lamp bu lb and thus accelerate the aging process of the lamp. 14. Plug a new halogen lamp to the panel. 15. Insert the halogen lam p with the half r[...]

11 6 Maintenance[...]

11 7 Section 8 T roubleshooting Problem/Dis play screen Likely Cause Action Attention! Please insert the light shield. Measurements with barcode cuvettes generally require the light shield. Insert the light shield. Press OK . Absorbance > 3.5! The measured abso rbance exceeds 3.5 Dilute the sample and repeat the measurement Concentratio n too hi[...]

11 8 T roubleshooting[...]

11 9 Section 9 Replacement Part s Description Cat. N o. Rechargeable lithium battery LZV551 T ungsten Lamp LZV565 Cuvette/ Sample cell adapter 10 mm (A) LZV583 Cuvette/ Sample cell adapter 1 inch (C) LZV584 Light Shield LZV646 Cell adapter for Pour-Thru Cel l (B) LZV585 Cell adapter set (cuvette adapters A, B, and C) LZV647 W all power supply LZV61[...]

120 Replacement Part s[...]

121 Section 10 Cont act Information HACH Comp any World Headquarters P .O. Box 389 Loveland, Colorado 80539-0389 U.S.A. T el (800) 227-HACH (800) -227-4224 (U.S.A. only) Fax (970) 669-2932 orders@hach.com www .hach.com Repair Service in the United St ates: HACH Company Ames Service 100 Dayton Avenue Ames, Iowa 50010 T el (800) 227-4224 (U.S.A. only[...]

122 Cont act Information[...]

123 Section 1 1 Limited W arranty Hach Company warrant s its product s to the original pu rchaser against any defect s that are due to faulty material or workmanship for a period of one ye ar fr om date of shipment unless otherwise noted in the product manual. In the event that a defect is d iscovered during the warranty period, Hach Compan y agree[...]

124 Limited W arranty[...]

125 Index A Absorbance Check ......... ................ ................ ....... 101 Absorbance Formula ................... ................ ...... 90 , 91 Alphanumeric keyp ad ............... ............. ................ .. 25 Audio signals ..................... ................ ................. ..... 29 B Barcode Prog rams ................ ...[...]

126 V Variables ............... ................ ................ ....... 55 , 57 , 78 Verification Kit ................. ................ ................ ....... 102 Version ........... ................. ................ ................ ... 55 , 56 View Table ...................... ................ ................ ......... 94 VIS-Lamp Lamp Con[...]