Leica ASP300 manuale d’uso

Instruction manual Leica ASP300 V1.3 English - 12/2003 Always keep this manual together with the instrument. Read this instruction manual carefully before working with the instrument. Leica ASP300 Advanced Smart Processor Automated vacuum tissue processor[...]

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3 Leica ASP300 NOTE The information, numerical data, notes and value judgments contained in this manual rep- resent the current state of scientific knowl- edge and state-of-the-art technology. We are under no obligation to update the present manual according to the latest tech- nical developments, nor to provide our cus- tomers with additional copi[...]

4 Instruction manual V 1.3 – 12/2003 Table of contents 1 . Important notes .............................................................................................................. ............... 6 1.1 Symbols used in this manual ................................................................................................... 6 1.2 Design[...]

5 Leica ASP300 Table of contents 5.2.6 Editing the stations .................................................................................................................. 4 6 5.2.7 Reagent groups ........................................................................................................................ 4 7 5. 3 Pr o gr am s .......[...]

6 Instruction Manual V 1.3 – 12/2003 1.1 Symbols used in this manual Warnings appear in a grey box and are marked by a warning triangle . Notes, i.e. important user information appear in a grey box and are marked by an information symbol . Flammable solvents and reagents are marked with this symbol. Instrument surfaces which become hot during ope[...]

7 Leica ASP300 2. Safety 2. 1 Safety instructions This instruction manual includes important in- formation related to the operating safety and maintenance of the instrument and it is an im- portant part of the product. If additional requirements on accident prevention and environmental protec- tion exist in the country of operation, this instructio[...]

8 Instruction manual V 1.3 – 12/2003 2. Safety 2. 2 Warnings The safety devices installed in this instrument by the manufacturer only constitute the basis for accident prevention. Primarily responsible for accident-free operation is above all the institution which owns the instrument and, in addition, the designated personnel who operates, servic[...]

9 Leica ASP300 Warnings - working with the instrument The Leica ASP300 may only be operated by trained laboratory personnel and as per the present instruction manual. In case of emergency switch off mains (item no. 5 in Fig. 3) at the rear of the instrument and unplug the power cord. The instrument must be installed so that the mains switch at the [...]

10 Instruction manual V 1.3 – 12/2003 2. 3 Integrated safety devices The instrument is equipped with the following protection features: • overpressure protection, • protection against excessive vacuum, • overcurrent protection, • overheating protection. Overpressure protection The design of the pressure system ensures that no dangerous le[...]

11 Leica ASP300 3. Instrument components and specifications 3. 1 Specific instrument options • R eagent M anagement S ystem (RMS). • The specimen cassette baskets may be used with or without cas- sette dividers and spring separators designed to separate cassettes and improve fluid penetration. • In-process fluid recirculation (‘tidal agitat[...]

12 Instruction manual V 1.3 – 12/2003 3. 2 Overview - instrument components 3. Instrument components and specifications 2 3 4 5 13 6 7 14 15 16 17 18 19 20 21 22 12 1 1 8 10 9 1 Fig. 1 23[...]

13 Leica ASP300 Overview - Instrument components 1 - Basic instrument - processor module 2 - Basic instrument - reagent module 3 - Wax bath lids 4 - Retort lid 5 - Touch screen 6 - Cover flap of instrument console 7 - Instrument console comprising: 8 - Printer port 9 - Local alarm connection 1 0 - Remote alarm connection 1 1 - Floppy disk drive 1 2[...]

14 Instruction manual V 1.3 – 12/2003 3. Instrument components and specifications 3. 3 Standard delivery - packing list Leica ASP300 basic standard delivery: 1 Leica ASP300 - Basic instrument 0476 34100 1 Accessory kit consisting of: 0476 35929 - 1 Basket hook 0476 34713 - 3 Specimen baskets, assy., including lid, dividers, springs and handle 047[...]

15 Leica ASP300 3. 4 Technical data Nominal voltage: Two factory-preset voltage settings (not user-adjustable): 100 to 120 V + 1 0% or 230 to 240 V + 1 0% Nominal frequency: 50 to 60 Hz Main fuses: 2 fast-blowing melting fuses, 20 x 5 mm, UL-listed Order no. • 100 to 120 V; F 10 A 250 VAC 69411002 • 230 to 240 V; F 5 A 250 VAC 69411000 Nominal [...]

16 Instruction manual V 1.3 – 12/2003 Retort Capacity: max. 300 cassettes Reagent volume (in liters): 4.3 Temperature (paraffin): 40 to 65 °C Temperature (processing reagents): ambient or 35 to 55 o C Temperature (cleaning reagents): 50 to 65 °C Temperature accuracy: + 1 K Filling time: approx. 90 sec Drain time : approx. 80, 120, 140 sec (adju[...]

17 Leica ASP300 3. Instrument components and specifications 3.4 Technical Data (continued) Hardware and Software: • Large color liquid crystal display (LCD) and touchscreen. • User-friendly, intelligent software. • 3,5“ floppy drive and printer port. • Alarm system with two remote alarm sockets. • Password-protected instrument superviso[...]

18 Instruction manual V 1.3 – 12/2003 3. 5 Compatible reagents Fixatives 1. Formalin, buffered or unbuffered 2. Formalin saline Dehydration 1. Ethanol 4. Butanol 2. Isopropanol 5. Industrial Methylated Spirits 3. Methanol Clearing 1. Xylen (recommended) 2. Toluol * 3. Chloroform * Paraffin wax 1. Paraffin Caution: Reagents other than those listed[...]

19 Leica ASP300 Moving the instrument The instrument must be installed such that the mains switch at the rear of the instrument (item no. 5 in Fig. 3) can easily be ac- cessed at any time. After the instrument has been unpacked (see unpacking instructions attached to outside of transport crate) it must be touched only at the points marked with dots[...]

20 Instruction manual V 1.3 – 12/2003 4. Setting up the instrument • Connect mains cable ( 6 ) to mains power supply socket ( 1 ) (see Fig. 3 ). • Jumper cable ( 3 ) connects the mains power supply output ( 2 ) to the electronics module input ( 4 ). • Switch ON/OFF switch ( 5 ) ON. • Switch ON/STOP switch at the right of the instrument ON[...]

21 Leica ASP300 4. Setting up the instrument • Unpack the activated carbon filter and in- sert it into the instrument (see Fig. 4 ). Make sure to insert the filter with the correct side facing upwards. See label ( 6 ) at the front of the filter - the arrow must point upwards. • Unpack the magnetic stirrer and insert into the retort. • Install[...]

22 Instruction Manual V 1.3 – 12/2003 4. 4 Setting up the data connections Printer (optional) • Lift off the instrument console flap ( 12 ). • Connect the printer to the printer port ( 7 ) via a standard Centronics cable (refer Fig. 6 ). Other connections • For additional data transfer and storage the instrument console is equipped with a s[...]

23 Leica ASP300 4. 5 Alarm functions The Leica ASP300 is equipped with two different alarm functions: Instrument alarm This alarm is generated from within the Leica ASP300. The instrument alarm is generated in all alarm situations. Remote alarm This alarm device is installed outside of the Leica ASP300. All alarms (all types of alarm) are forwarded[...]

24 Instruction Manual V 1.3 – 12/2003 • After switching it on the instrument will take a few minutes to initialize (see opposite - Fig. 7 ). • After initialization, the FAVORITES screen will be displayed ( Fig. 8 ). Screen saver • A screen saver will operate turning the screen off when no key has been pressed for a (user-) programmable time[...]

25 Leica ASP300 System diagnostics menu If, during initialization, ‘Touch here ...’ is touched (refer Fig. 7 ) and sub- sequently the supervisor pass- word is entered, the SYSTEM DIAGNOSTICS menu ( Fig. 9 ) opens up, giving access to standard in- strument settings. Attention! Serious instrument malfunctions may occur if any settings are modifie[...]

26 Instruction manual V 1.3 – 12/2003 4. 7 Touch screen functions 4.7.1 User interface - overview The Leica ASP300 is programmed and operated via a colored LCD touch screen. An on-line help feature provides detailed information on all instrument functions. HELP can be accessed from any screen by pressing the HELP button. Button symbols To activat[...]

27 Leica ASP300 4.7.2. Access control The Leica ASP300 may be configured to allow two levels of user ac- cess. Operator access level: • Operators may run programs and view results. On this level, the OP- ERATOR symbol is displayed in the upper right corner of the touch screen. Supervisor access level: • Supervisors may perform all Operator func[...]

28 Instruction manual V 1.3 – 12/2003 4. Setting up the instrument 4.8 Check list for first operation (at supervisor access level) Parameter settings Enter instrument name and select lan- guage. Activate printer (optional). Enter carbon filter age warning and pump warning threshold. Set all parameters as required, especially wax bath set temperat[...]

29 Leica ASP300 5. 1 Reagent handling 5.1.1 Fill/drain reagents (other than wax) 1. Manually changing the reagents • Remove the appropriate reagent bottle from the Leica ASP300 re- agent cabinet, remove the bottle screw cap. • Drain/refill the reagent by pouring to/from a bulk container. • On the REAGENT STATUS screen, verify correct allocati[...]

30 Instruction Manual V 1.3 – 12/2003 Select station Press key symbol Fig. 12 → 5. Operation Remote drain • On the screen, select reagent bottle no. 3 and the bulk container ( Remote ). The press STATION DRAIN . • Check for leaks. Remote fill • On the screen, select reagent bottle no. 3 and the bulk container ( Remote ). The press STATION[...]

31 Leica ASP300 5. Operation 5.1.2 Changing the wax Draining the wax • Connect the paraffin drain hose ( 2 ) as shown in Fig. 13 and insert the loose end of the hose into a remote collecting container. Fig. 13 • Select the wax bath to be drained and the remote container (‘Remote’). Then press STATION DRAIN . • Several queries will be disp[...]

32 Instruction Manual V 1.3 – 12/2003 Fig. 14 → → • Remove the splash protector. • Fill the wax bath ( Fig. 15 ) with wax pellets up to the top. • Press BACK and REAGENT STATUS to ac- cess the reagent status table ( Fig. 14 ). High- light the wax bath you just filled and press SET AS FULL . • After about 90 minutes, add another 0.5 kg[...]

33 Leica ASP300 Fig. 16 → → Adding molten paraffin • Molten wax may have a maximum temperature of 70 ° C. • If filling with molten wax, do not fill above the MAX level (see Fig. 15 ). • After a wax bath has been filled, it must be marked as ‘full’ in the REAGENT STATUS screen. Software control • If the wax baths are filled with mol[...]

34 Instruction manual V 1.3 – 12/2003 5. 2 Setting up the instrument parameters From the start screen, press MENU to go to the MENU FUNCTIONS screen, then press MORE . In the MORE MENU FUNCTIONS screen, press SYSTEM SETUP . 5.2.1 System setup → 5. Operation SYSTEM SETUP screen (Fig. 17) . The DISPLAY/BUZZER field is practically self-explanatory[...]

35 Leica ASP300 Program Options Selection of the options on how programs are carried out. • STATION ALLOCATION : By age or Sequential By age - RMS is activated, reagents will automatically be used in order of increasing cleanliness. Sequential - reagents will be used in sequential order of stations. • WAX BATH ORDER : Auto or 1. ; 2. ; 3. Wax b[...]

36 Instruction manual V 1.3 – 12/2003 Security To determine the level of access to the instrument. • SUPERVISOR PASSWORD : Enabled or Disabled Enabled - A password is required for access to the supervisor level. Disabled - All instrument and software functions are fully accessible to all staff. • LOCK MODE : Enabled or disabled. Enabled - No [...]

37 Leica ASP300 5.2.2 Editing the reagent list Adding new reagents 1. Enter the reagent name and allocate the new reagent to the reagent group it belongs to. 2. Define the stations (bottles) that will be filled with the new reagent. 3. Fill the stations (bottles) according to the reagent list. Enter reagent names Enter reagent names via the REAGENT[...]

38 Instruction manual V 1.3 – 12/2003 Allocation of reagents to the correct reagent group is the basis for compatibility monitoring. Allocation to the wrong reagent group can lead to reagent cross contamination. Entering / modifying reagent thresholds • Highlight the reagent to be modified. • Press the headline key symbol of the parameter to [...]

39 Leica ASP300 Deleting reagents • Highlight the reagent to be deleted in the SET UP REAGENTS AND WARNING THRESHOLDS screen. • Press DELETE . • Press OK in the screen prompt. Please remember that a reagent which is already used in a pro- gram cannot be deleted. Adding new reagents to stations Go to SMART FUNCTIONS to remote-fill the reagent [...]

40 Instruction manual V 1.3 – 12/2003 5. Operation 5.2.3 V ie wi ng t he pr og ra m list This list ( Fig. 20 ) displays all programs currently existing in the ASP300. You can create: • up to 15 processing programs, • 3 retort cleaning programs, • 1 wax cleaning program. At supervisor access level: • the processing program names can be edi[...]

41 Leica ASP300 5. Operation 5.2.4 Adding and/or modifying programs Creating a new program • You must be logged on at supervisor level. • In the VIEW/EDIT PROGRAMS screen ( Fig. 20 ) highlight a program as similar as possible to the program you wish to create. • Press COPY to copy the selected program. The new program will have the same name [...]

42 Instruction manual V 1.3 – 12/2003 5. Operation Fig. 21 Editing program steps • To edit a step, highlight the corresponding line and press the re- spective headline. • In the entry windows that pop up enter / select the program step set- tings. ↓ ↓↓ ↓ ↓[...]

43 Leica ASP300 Editing program steps (continued) Setting a delay step A so-called ‘delay step’ ensures that a program is finished at a specified time. • Highlight the program step you wish to define as a delay step. • Press DELAY . The delay symbol is moved to the selected step, thus defining the step as delay step. Copying steps • Highl[...]

44 Instruction manual V 1.3 – 12/2003 5.2.5 Favorites (maximum of 5 programs) Favorites can be programmed to end either: • “as soon as possible“ (ASAP) or • at a predetermined finish time. Adding and/or modifying Favorites • Highlight the program line to be altered. • Press PROGRAMS . • Select the desired program and press OK to con[...]

45 Leica ASP300 Assigning a finish day • Press DAY . • Select the desired week day in the list and press OK to confirm. Assigning an icon to a “Favorite“ • Press SYMBOL in the table header. • Select the desired icon and press OK to confirm. The first icon on the list is empty - select this option, if you do not want to assign an icon to[...]

46 Instruction Manual V 1.3 – 12/2003 Fig. 23 ↓ → → Adding / changing reagents Highlight the desired station. • Press REAGENT NAME . • The SELECT REAGENT entry window pops up. Only those reagents that are compat- ible with the respective station will be available for selection. • Highlight the desired reagent and press OK to confirm. [...]

47 Leica ASP300 Fig. 24 ↓ → → 5.2.7 Reagent groups Selecting a new color for a reagent group • Highlight the line containing the reagent color you wish to change. • Press COLOR in the table header: the entry window SELECT COLOR pops up. • Select the desired color and press OK to confirm. • If you want to close the entry field without [...]

48 Instruction Manual V 1.3 – 12/2003 Fig. 25 Fig. 26 5. 3 Programs Programs can be started from two different screens: In the Favorites screen, press ALL PROGRAMS . A ”Favorite” is a processing program that is frequently used. All settings are already programmed. Only the number of blocks has to be entered (if this function is enabled). Star[...]

49 Leica ASP300 Fig. 28 ↓ Fig. 29 ↓ Fig. 27 5.3.1 Editing a selected program To modify a program prior to starting it, press EDIT to access the Edit Program screen. ↓ Redefine the program finish time: • Press FINISH TIME . In the entry window, set a new finish time. ↓ • Enter the day when you want the program finished. • Enter the fin[...]

5. Operation Access the RUN OPTIONS function to display the options selected for the current program. • LEVEL CHECK enabled: The lower level sensor in the retort is acti- vated. • RECIRCULATION enabled: During each processing step, the reagent is pumped periodically from the bottle to the retort and back for continuous and efficient mixing of f[...]

51 Leica ASP300 • Press START to start the program. Press OK to confirm the query that follows. Enter the number of blocks, if the NUMBER OF BLOCKS op- tion has been enabled. • The FINISH TIME button shows the time when the current program will be finished. • Before a program in progress can be modified, the PAUSE symbol must be pressed. To m[...]

52 Instruction Manual V 1.3 – 12/2003 → 5. 4 Reagent status In the reagent status screen ( Fig. 32 ) you can display and update the actual reagent status of each station and you can set the stations as full or empty. Fig. 32 • Warning thresholds that trigger warning messages appear on a red background. • To change a reagent status, highligh[...]

53 Leica ASP300 5. Operation 5. 5 System Monitor The SYSTEM MONITOR screen supplies important infor- mation on the Leica ASP300 system status. After replacing the filter press RESET . ROTARY VALVE RETRIES: If a figure higher than 50 is indicated, a preventive maintenance is recommended. FILL/DRAIN: This screen displays the average fill / drain time[...]

54 Instruction Manual V 1.3 – 12/2003 Detailed information on how to create and install customer-specific help files can be found in the “What is Custom Help?“ help text. Accessing ‘Help’ in the event of error messages • Press DETAILS , or • Press the HELP button on the error message. 5. 6 On-line help The Leica ASP300 features an ext[...]

55 Leica ASP300 → → ↓ ↓ Printing run logs Printing is possible when the PRINT symbol is displayed. For the PRINT symbol to be displayed, a printer must have been configured for the instrument. The following lists and protocols can be printed: • reagent list, • stations list, • reagent status list, • all programs, • run log, • er[...]

56 Instruction manual V 1.3 – 12/2003 6. 1 General The Leica ASP300 is equipped with a trouble shooting system. If an error is detected, detailed error messages and corresponding user instructions on what to do are displayed on the touch screen. For all correctable error conditions the instrument will take corrective actions to protect the specim[...]

57 Leica ASP300 → 6. 3 Trouble shooting • Check for error messages indicating the nature of the problem. • Press the Help button on the message for any information relating to the cause. • Check the Run Log for any information on the nature of the failure. Use the SMART SCREEN functions to individually test opera- tions such as fill, drain,[...]

58 Instruction manual V 1.3 – 12/2003 6. 4 Typical fill or drain problems Fill and drain problems may be due to several causes: 1. There is insufficient reagent • Verify that both the reagent bottles and the wax stations have sufficient reagent. 2. Insufficient pressure or vacuum The Leica ASP300 is unable to create sufficient vacuum (for a fil[...]

59 Leica ASP300 7. Cleaning and maintenance 7. 1 Clean programs You find the clean programs at the end of the programs list ( Fig. 35 ). To display a clean program, highlight the re- spective line and press EDIT . The ASP300 offers three retort clean programs and one paraffin clean program. Clean programs cannot be copied or deleted. 7.1.1 Retort c[...]

60 Instruction manual V 1.3 – 12/2003 Editing steps • To edit a program step highlight the corresponding line and press the respective table header. • To disable a step, set the number of cycles to “ 0 “. Only cleaning wa- ter steps can be disabled. The following characteristics of each step can be edited: • Reagent name - only those re[...]

61 Leica ASP300 → → 7.1.2 Wax clean programs The Leica ASP300 contains a wax clean fea- ture that removes solvents ( xylene ) from used wax. Follow the instructions on the left ( Fig. 36 ) to run a wax clean program. The duration of the wax clean program is user-programmable. Follow the instructions in Fig. 37 . Fig. 36 Fig. 37 7. Cleaning and [...]

62 Instruction manual V 1.3 – 12/2003 7.1.3 Smart clean The Smart Clean prepares the ASP300 for transport. Prior to starting a Smart Clean, a complete retort clean program must be run. To start a Smart Clean, press SMART CLEAN on the MORE MENU FUNC- TIONS screen and press OK to confirm. The Smart Clean takes approx. 15 minutes and consists of 3 p[...]

63 Leica ASP300 7. 2 General cleaning steps Paraffin wax baths Warning! Be careful as the walls of the wax baths are very hot and may cause burns! • Wipe the wax baths and lids clean. The lid can be removed for cleaning purposes. • If they are dirty, remove the wax strainers from the wax baths. Clean, dry and reinsert them. • Ensure that the [...]

64 Instruction Manual V 1.3 – 12/2003 Exchanging the activated carbon filter • Exchange the filter as shown in Fig. 40 . • Replace the filter at least every four months – make sure to insert correctly – (see arrow on label)! • Perform a Smart Clean prior to changing the filter. Fig. 40 Draining the condensate bottle • Remove and empty[...]

65 Leica ASP300 7.3.1 Daily cleaning and maintenance Cleaning the retort lid • Remove wax from the inside of the retort lid with the plastic scraper provided. Make sure to remove all wax from around the retort lid seal. • For convenience, the lid may be removed during cleaning by lifting it vertically and sliding it towards the console. Cleanin[...]

66 Instruction manual V 1.3 – 12/2003 This procedure is particularly important with bottles containing chloroform. Chloroform tends to swell the bottle seals, making them difficult to remove. This problem can be prevented by regular lubrication. Checking the retort and wax bath lid seals • Regularly check the retort and wax bath lid seals for d[...]

67 Leica ASP300 7. Cleaning and maintenance Maintenance activity daily w eekly monthly every 4 months Lubricate reagent bottle O-rings √√ ∗ and check for damage. Wipe dry retort and lid √ after completing retort clean cycle. Clean exterior of instrument √ with soft cloth and minimal amount of xylene. Check retort strainer √ for any tiss[...]

68 Instruction manual V 1.3 – 12/2003 7. Cleaning and maintenance Maintenance activity daily w eekly monthly every 4 months Inspect and, if necessary, √ clean wax bath strainers . Inspect wax bath air vent and, √ if necessary, clear. Inspect and empty condensate bottle. √ Clear inlet holes. Examine activated carbon filter. √ Replace carbo[...]

69 Leica ASP300 8. Warranty and service Warranty Leica Microsystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality con- trol procedure based on the Leica in-house testing standards, and that the product is faultless and complies with all technical specifications and/or agreed characteri[...]

70 Instruction manual V 1.3 – 12/2003 Notice[...]