Leica CM3600 manuale d’uso

Leica CM3600 Cryomacrotome Instruction manual Leica CM3600 English V 6.0 08/2006 Always keep this manual near the instrument. Read carefully prior to operating the instrument Order No.: 0708 35101[...]

2 Instruction manual V 6.0 - 08/2006 Published by: Leica Microsystems Nussloch GmbH Heidelberger Str. 17 - 19 D-69226 Nussloch Germany Telephone: +49 62 24 143-0 Facsimile: +49 62 24 143-2 00 E-mail: hi sto_info@leica-microsystems.com Internet : http://www.histo-solutions.com . These are determined only by the contract pro- visions agreed between o[...]

3 Leica CM 3600 – Cryomacrotome EC Declaration of conformity We herewith declare in exclusive responsibility that the Leica CM 3600 – Cryomacrotome was developed, designed and manufactured to conform with: Council Directive 89/392/EEC, Appendix II A (Machines), Council Directive 73/23/EEC (low voltage) and Council Directive 89/336/EEC, Appendix[...]

4 Instruction manual V 6.0 - 08/2006 Index 1. Important notes ................................................................................................................... 8 1.1 Symbols in the text and their meaning ....................................................................................... ..................................... 8 [...]

5 Leica CM 3600 – Cryomacrotome Index 5.3.2 Fuses ......................................................................................................................................................... 31 5.4 PC ......................................................................................................................................[...]

6 Instruction manual V 6.0 - 08/2006 Index 6.12 Screen printout ................................................................................................................................................................... 8 3 6.13 Remote inquiry ..................................................................................................[...]

7 Leica CM 3600 – Cryomacrotome Index[...]

8 Instruction manual V 6.0 - 08/2006 1.1 Symbols in the text and their meaning Notes, i.e. important user information appear in a grey box and are marked with an information symbol . Buttons and function keys which need to be pushed on the data entry screen, are printed in bold capitals. Figures in brackets refer to item num- bers in drawings. (5) [...]

9 Leica CM 3600 – Cryomacrotome 1.4 Designated use The Leica CM3600 is an SPS-controlled and motorized precision cryomacrotome for large-surface sections, equipped with a convection- cooled cryochamber for deep-freezing, sectioning, storing and freeze- drying large biological and industrial specimens. The instrument is designed for the above desc[...]

10 Instruction manual V 6.0 - 08/2006 1.5 Warranty Leica Microsystems Nussloch GmbH guarantees that the contractual prod- uct delivered has been subjected to a comprehensive quality control proce- dure based on the Leica in-house testing standards, and that the product is faultless and complies with all technical specifications and/or agreed char- [...]

11 Leica CM 3600 – Cryomacrotome 1. Important notes[...]

12 Instruction manual V 6.0 - 08/2006 2.1 Layout of the CM3600 PC Knee lever Window Chamber lid Emergency stop switch Port and switch panel Chamber Refrigeration and control unit Socket panel Microtome (in chamber) Port and switch panel Drain tap 2. Characteristics of the Leica CM3600[...]

13 Leica CM 3600 – Cryomacrotome Drain tap Condensation water is drained through the drain tap. Refrigeration and control unit The refrigeration and control unit cools the cryochamber and controls the microtome and the entire electronics. Knee lever The knee lever is used for manual operation of the specimen sledge. Socket panel The PC and periph[...]

14 Instruction manual V 6.0 - 08/2006 2.2 Technical Data 2.2.1 Microtome type 450 C Sliding microtome Section thickness selection: 1 - 200 μm Sectioning range: 0 - 450 mm Max. sectioning speed: 80 mm/s Knife retraction after sectioning stroke: 50 - 250 μm Total vertical stroke of knife: 70 mm (standard) Total vertical stroke of knife: 100 mm (mod[...]

15 Leica CM 3600 – Cryomacrotome 2.2.3 Dimensions and weight Inner (cryochamber L x H x W): 1650 x 720 x 600 mm Outer (L x H x W): 2800 x 1090 x 920 mm (Overall dimensions with connecting cables) Outer length (total): 2800 mm Outer length (chamber only): 1940 mm Outer length (control unit with connections only): 860 mm Outer height (total): 1090 [...]

16 Instruction manual V 6.0 - 08/2006 200 V / 50 Hz, two-phase Nominal voltage: 200 V AC ± 10 % Nominal frequency: 50 Hz Power draw: 2300 VA Automatic cutout/ 2 x 8A / 1 x 10A Mains safety switch UL-listed 200 V / 60 Hz, two-phase Nominal voltage: 200 V AC ± 10 % Nominal frequency: 60 Hz Power draw: 2300 VA Automatic cutout/ 2 x 8A / 1 x 10A Main[...]

17 Leica CM 3600 – Cryomacrotome 2.2.6 General parameters Protective class: I Pollution degree: 2 Overvoltage installation category: II 2.3 Instrument parts The Leica CM3600 consists of a sliding microtome in a large-volume cryochamber. The instrument is designed for cryosectioning large speci- mens or for processing specimens with the so-called [...]

18 Instruction manual V 6.0 - 08/2006 Prior to each sectioning stroke, a stepper motor feeds the microtome knife downwards towards the specimen by a selected amount of microns (section thickness). During each return stroke of the specimen sledge, the stepper motor automatically carries out a knife retraction movement, programmable between 50 and 25[...]

19 Leica CM 3600 – Cryomacrotome 2. Characteristics of the Leica CM3600[...]

20 Instruction manual V 6.0 - 08/2006 3. Safety 3.1 Safety instructions This instruction manual contains important in- structions and information regarding the opera- tional safety and maintenance of the instrument. The instruction manual is an important part of the product, which must be read carefully prior to setup and use and must always be kep[...]

21 Leica CM 3600 – Cryomacrotome 3.3 Safety standards The Leica CM3600 was built and tested in accordance with the safety regulations for electrical measuring, control, regulating and laboratory devices listed below: • DIN EN 292, • DIN EN 55011, • DIN EN 61010-1, • IEC 1000-4, • IEC 50082-1, • MedGV. In order to maintain this conditi[...]

22 Instruction manual V 6.0 - 08/2006 3.4.2 Knee lever Never leave the instrument unattended when the knee lever is folded out ( 1 ) to prevent the microtome from being set in motion acciden- tally. Always swing the knee lever upwards before leaving the instru- ment. 3.4.3 Window The window is heatable and equipped with a lock button ( 2 ). To open[...]

23 Leica CM 3600 – Cryomacrotome 3.5 Special safety instructions The safety devices installed in the Leica CM3600 by the manufacturer only constitute the basis for accident prevention. Primarily responsible for accident-free operation is above all the institu- tion which owns the instrument and, in addition, the designated personnel who operates,[...]

24 Instruction manual V 6.0 - 08/2006 As you work through the instruction manual, familiarizing yourself with the instrument, do not yet install a knife or specimen. Below you find a summary of all warnings contained in this instruction manual. Any use of the instrument not described in this instruction manual is considered improper. Always swing t[...]

25 Leica CM 3600 – Cryomacrotome Prior to any work involving the knife, microtome or inside the cryochamber, swing the knee lever upwards. For any work involving the knife, microtome or inside the cryochamber always wear special protective gloves. For reasons of safety and accuracy, the maximum trimming thickness should not exceed 100 μm per sec[...]

26 Instruction manual V 6.0 - 08/2006 4. Site requirements 4.1 Site requirements at place of installation If possible at all, the room should be air-conditioned. Relative humidity ≤ 60 %. Room temperature should not exceed 22 °C. If ambient conditions are not maintained as specified, instrument perfor- mance may be negatively affected (lowest sp[...]

27 Leica CM 3600 – Cryomacrotome For removing the microtome (approx. 190 kgs) from the cryochamber, a ceiling crane capable of carrying that weight should be available. If no ceiling crane is installed yet, we recommend the special Leica crane „KR-CM3600“. 4.2 Electrical connections Country: Europe USA Japan Installation cord cross section: 2[...]

28 Instruction manual V 6.0 - 08/2006 5.1 Unpacking and installation Only duly trained personnel may unpack and install the Leica CM3600. Please contact your local Leica sales organization for appropriate advice. 5.2 Standard delivery The standard delivery includes: • 1 Leica CM3600, consisting of: • Cabinet • Refrigeration and control unit ?[...]

29 Leica CM 3600 – Cryomacrotome 5.3 Port and switch panel Control lamps Connecting block Mains switch Automatic cutout Ground fault circuit interruption Connection for compressed air Connection for foot switch Interface RS 232 Switch for socket panel External alarm signal switch Connection for external alarm Socket panel Control lamp 5. Installa[...]

30 Instruction manual V 6.0 - 08/2006 5.3.1 Port and switch functions Mains switch Switch in position “0“ - instrument disconnected from mains except for the socket panel. Socket panel switch Control light is on (red), when the socket panel switch is ON. Foot switch connection Optionally, a foot switch can be connected for the purpose of sectio[...]

31 Leica CM 3600 – Cryomacrotome GFCI switch Protects the entire instrument. Automatic cutout Protects the refrigerating sets and all electronic components. 5.3.2 Fuses The Leica CM3600 is equipped with the following fuses: • GFCI switch • Automatic cutout • Fuse protecting the mains switch • Fuse protecting the socket panel GFCI switch A[...]

32 Instruction manual V 6.0 - 08/2006 Fuse protecting the mains switch If the mains switch fuse has blown, please call Technical Service. S Fuse protecting socket panel If the socket panel fuse has blown, please proceed as follows: Prior to exchanging the fuse in the socket panel, switch the instrument mains switch off and unplug the instrument fro[...]

33 Leica CM 3600 – Cryomacrotome 5.4 PC The Leica CM3600 is equipped with a commercial PC, monitor, keyboard, mouse and an internal modem. For further information, please refer to the manual of the PC. The control PC as well as all peripherals connected to it (printer, monitor, etc.) are set to an input voltage of 230V/50Hz. For reasons of compat[...]

34 Instruction manual V 6.0 - 08/2006 6. Software 6.1 Start and log-in procedure Requirements: PC must be on and the Microsoft Windows XP ® operating system must have booted and be ready for service. Doubleclicking on Icon „CM3600“ starts the application. Registration mask 1. Type the user name in the identification field. 2. Type the correct [...]

35 Leica CM 3600 – Cryomacrotome 6.2 Description of the window elements The window surface is divided into several areas: • Title bar • Tool bar • Work space • Status bar 6.2.1 Title bar The „Title bar“ displays information on instrument name and software version number. 6.2.2 Tool bar The „Tool bar“ provides quick access (via mou[...]

36 Instruction manual V 6.0 - 08/2006 Icons Clicking on the icon opens the „Main window“, which also opens up after completion of the start and log-in procedure. Clicking on the icon opens the „Parameter settings“ window. Clicking on the icon opens the „Temperature curve“ window. Clicking on the icon opens the „Chronological event lis[...]

37 Leica CM 3600 – Cryomacrotome Clicking on the icon opens the „Label printing“ window. When activated, the icon will light up in green. Clicking on the icon activates the „Foot switch“. When acti- vated, the icon will light up in green and the current sectioning data will be saved. The ‘Stop’ icon appears immediately when the emerge[...]

38 Instruction manual V 6.0 - 08/2006 6.3 Initialization To be able to work with the instrument, after completion of the start and log-in procedure the microtome must be initialized. For initialization, proceed as follows: 1. In the „Main window“, click on the INIT button blinking in yellow color (in ‘Mode’ field). The „Safety check - ini[...]

39 Leica CM 3600 – Cryomacrotome Once initialization has been started, it can only be interrupted by pressing the emergency stop switch. For further informa- tion, refer to chapter 3.4.1. After the initialization procedure has been completed, the color of the INIT button will first change from yellow to green and after a few sec- onds, the captio[...]

40 Instruction manual V 6.0 - 08/2006 6.4 Main window The main window either opens up automatically after comple- tion of the start and log-in procedure or by clicking on the „Main window“ icon. The work space of the main window is divided as follows: • Chamber temperature • Time • Automatic defrost • Automatic dehydration • Knife mov[...]

41 Leica CM 3600 – Cryomacrotome 6.4.1 Chamber temperature Indication of set or actual chamber temperature. See chapter 6.5.1 ‘Parameter settings’ on how to set the chamber tem- perature. 6.4.2 Time Indication of real time and date. See chapter 6.5.1 ‘Parameter settings’ on how to set the time. 6.4.3 Automatic defrost cycle Indication of [...]

42 Instruction manual V 6.0 - 08/2006 6.4.4 Automatic dehydration Indication of start time, start date and duration of the automatic dehydra- tion. See chapter 6.5.1 Parameter settings on how to set the automatic dehy- dration parameters. MANUAL DEHYDRATION button Clicking on this button immediately starts a dehydration cycle. Clicking on this butt[...]

43 Leica CM 3600 – Cryomacrotome The red „Retraction“ caption is displayed only during the return stroke of the sledge. The ‘Knife movement’ icons can be activated only in MANUAL Mode. For further information, see chapter 6.4.9. The entry limit for actual and set position may vary between 70 - 200 μm, depending on the instrument version.[...]

44 Instruction manual V 6.0 - 08/2006 KNIFE DOWN button on the right Clicking on this button moves the knife holder fast downwards for a short while. If you click on and hold this button, the knife holder will continue to move downward until the left mouse button is released. While active, the button blinks in yellow color. PRESELECTION button Clic[...]

45 Leica CM 3600 – Cryomacrotome 6.4.6 Extraction system The extraction function can only be activated when an extrac- tion unit is connected to the instrument. For further informa- tion, refer to chapter 9.1. Button EXTRACTION ON/OFF in continuous mode Clicking on this button activates or deactivates continuous extraction. When activated, the bu[...]

46 Instruction manual V 6.0 - 08/2006 Click on the activated set value field to modify the setting. PAUSE button Clicking on this button stops the sectioning program. Clicking on the button once more restarts the sectioning program, resuming the current section where if was left off. 6.4.8 Sledge speed Indication of current sectioning or return str[...]

47 Leica CM 3600 – Cryomacrotome 6.4.9 Mode of operation To be able to work with the instrument, after completion of the start and log-in procedure the microtome must be initialized. For further informa- tion, refer to chapter 6.3. Buttons prior to initialization Buttons after initialization INIT button Clicking on INIT opens the ‘Initializatio[...]

48 Instruction manual V 6.0 - 08/2006 6.4.10 Cutting window Indication of the left and right block edge, graphically displayed by blue lines. They indicate the maximum horizontal stroke of the microtome sledge on a scale from 0 - 500 mm. Indication of the actual position of the microtome sledge. The sledge position is graphically displayed by the g[...]

49 Leica CM 3600 – Cryomacrotome 6.5 Parameter settings Click on „Parameter settings“ to open the corresponding window. The work space of the parameter settings window is divided as follows: • Parameter setting • Password list • Configuration • Reference voltages • Language selection • Initialization 6. Software[...]

50 Instruction manual V 6.0 - 08/2006 6.5.1 Parameter setting Set chamber temperature Click on this indication to change the currently selected value. Tempera- ture values from -30 to +1° C can be selected. Alarm temperature Click on this indication to change the currently selected value. Tempera- ture values from -25 to -5° C can be selected. Th[...]

51 Leica CM 3600 – Cryomacrotome Time and date Indication of time and date. Clicking on this indication opens the dialog box „System time“. Clicking on individual parameters (day, month, year, hour or minutes) the corresponding setting can be changed. Click OK to confirm. Start time 1 defrost Indication of start time of the 1st automatic defr[...]

52 Instruction manual V 6.0 - 08/2006 Dehydration date Dehydration start time Dehydration duration Indication of start date and time, and duration of the automatic dehydra- tion cycle. Clicking on one of the parameters opens the dialog box „Dehy- dration time“. Click on individual parameters (day, month, year, hour or minutes) to change the cor[...]

53 Leica CM 3600 – Cryomacrotome Cutting speed Return speed Indication of cutting and return speed. Click on one of the indications to change the currently selected value. Values from 0 - 80 mm/sec can be selected. Left block edge Right block edge Indication of left and right block edge. Click on the individual indication to change the currently [...]

54 Instruction manual V 6.0 - 08/2006 6.5.2 Password list To access the ‘User configuration’, the registered user must be an administrator. The „user configuration“ indication consists of the following columns: • No. Indication of the program-controlled running number. • Active Indication of status of the user selected. Active users are[...]

55 Leica CM 3600 – Cryomacrotome CLOSE button Clicking on this button closes the ‘User configuration’ window. Doubleclicking on a line in the configuration indication opens the dialog box „User configuration“. In the „user configuration“ dialog box the following settings can be selected: • No. Indication of the running number. No ch[...]

56 Instruction manual V 6.0 - 08/2006 • Access rights Click on the USER RIGHTS line to open a drop down menu. User property ‘Administrator’ or ‘User’ can be selected. Administrators have full access to all application functions. Users do not have access to the password list and to the configuration parameters in the “Parameter settings?[...]

57 Leica CM 3600 – Cryomacrotome 6.5.3 Configuration To access ‘Configuration’, the registered user must be an administrator. The „Configuration“ window contains the following tabs: • Text main study • Text substudy • Measured values • General • Labels • Comments OK button Clicking on this button saves the entries and closes t[...]

58 Instruction manual V 6.0 - 08/2006 Tabs „Text main study“ and „Text substudy“ The structure of the two tabs is identical. A header and 7 freely selectable comments for each main and/or substudy can be defined. The text entered into the 8 entry fields is copied into the dialog box for creating main and substudies. Each individual text can[...]

59 Leica CM 3600 – Cryomacrotome For the configuration of a main or substudy see chapter 6.10. The following measured values can be selected: • Set chamber temperature • Actual chamber temperature • Alarm temperature • Set section thickness • Actual section thickness • Knife holder position • Dehydration • Comment Tab „General?[...]

60 Instruction manual V 6.0 - 08/2006 Company/lab Enter the name of the company or the laboratory. The name will be displayed in the „information bar“, on the right side, see chapter 6.2.4. Printer for label printing Clicking on this line opens a drop down menu. If a printer for label printing is connected, it can be selected in this menu. Font[...]

61 Leica CM 3600 – Cryomacrotome Die export file is filed as a text file and according to the export path selected. Millitron Provided an external Millitron measuring device is connected to the PC, the corresponding serial connecting port can be selected via the drop down menu. Activating the millitron Clicking on the check box activates the exte[...]

62 Instruction manual V 6.0 - 08/2006 The following information can be represented as variables: @a Main study - name @b Main study - comment line 1 @c Main study - comment line 2 @d Current date (date) @e Main study - comment line 3 @f Main study - comment line 4 @g Main study - comment line 5 @h Main study - comment line 6 @i Main study - comment[...]

63 Leica CM 3600 – Cryomacrotome „Comments“ tab Frequently used comments can be entered into this tab. Button NEW Clicking on this indication opens the dialog box ‘Comment on section’ Here you can enter a comment on a section. Clicking on OK saves the entry and closes the window. Clicking on Cancel closes the ‘Comment on section’ dial[...]

64 Instruction manual V 6.0 - 08/2006 6.5.4 Reference voltages Indication of the current reference voltage in Volt. Buttons 1 to 8 Clicking on these buttons changes the reference voltage. The individual reference voltage values are predefined and cannot be changed. The display will read the new selected value. Activated buttons will light up in gre[...]

65 Leica CM 3600 – Cryomacrotome 6.6 Temperature curves Click on the temperature curve symbol to open the correspond- ing window. The window contains the following window panes: • Work space • Diagram The „Temperature curves“ window is only for failure analysis by Leica Service engineers. 6. Software Work space Diagram[...]

66 Instruction manual V 6.0 - 08/2006 6.6.1 Work space REFRESH button Clicking on this button updates the diagram display. RULER button If you click on this button, a ruler will be displayed in the diagram. Use the mouse cursor to move ruler to a certain measuring value position in order to read the measured value more exactly. PRINT button Clickin[...]

67 Leica CM 3600 – Cryomacrotome Clicking on one of the check boxes in the work space, the 8 temperature sensors of the instrument can be displayed individually in the diagram. The font color of the check boxes helps to identify the corresponding measured values in the diagram, which are displayed in the same color. The following check boxes can [...]

68 Instruction manual V 6.0 - 08/2006 6.6.2 Diagram The diagram indicates the following: • Temperature in degree centigrade on the y-axis • Time and date on the x-axis • Graphical representation of the 8 temperature measuring points in the instrument by colored lines The diagram can be enlarged as follows: 1. Within the diagram, click and hol[...]

69 Leica CM 3600 – Cryomacrotome 6.7 Chronological event list Click on „Event list“ to open the corresponding window. The events list is a table displaying the events chronologically, in order of date and time. In this list all events concerning the program are saved. The work space is divided up as follows: • Work space • Event table 6. [...]

70 Instruction manual V 6.0 - 08/2006 6.7.1 Work space FILTER button Clicking on this indication opens the dialog box „Filter“ Here you can define a time filter limiting the event table indication to one day, one week or one month. To set a filter, proceed as follows: 1. Click on one of the check boxes DAY , WEEK or MONTH to define the desired [...]

71 Leica CM 3600 – Cryomacrotome Comment Clicking on an event in the events list activates the „Comment“ field. You can enter a comment regarding the selected event. After confirmation with the ENTER key, the note will be displayed in the event table. Number Number of events displayed in the events list. Print button Clicking on this button c[...]

72 Instruction manual V 6.0 - 08/2006 6.7.2 Event table The table is divided into the following columns: • Date/time Date and time of the events. • User Name of the user who was logged in when the event occurred. • Message Event messages (short description of event). • Comment Comments on the events. For further information, refer to chapte[...]

73 Leica CM 3600 – Cryomacrotome 6.8 Alarm list Click on the „Alarm list“ symbol to open the corresponding window. Chronological display of the alarm list as a table, in order of date and time. In this list, all events concerning the program are saved. The window contains the following window panes: • Work space • Event table 6. Software [...]

74 Instruction manual V 6.0 - 08/2006 6.8.1 Work space In the work space the following parameters can be selected: FILTER button Clicking on this indication opens the dialog box „Filter“ For further information, refer to chapter 6.7.1. Comment Clicking on an event in the alarm list activates the ‘Comment’ field. You can enter a comment rega[...]

75 Leica CM 3600 – Cryomacrotome 6.8.2 Event table The table is divided into the following columns: • Occurred Indication of date and time when the alarm started • Cleared Indication of date and time when the alarm stopped • Confirmed Indication of date and time when the registered user confirmed the alarm message. For further information, [...]

76 Instruction manual V 6.0 - 08/2006 6.8.3 Error codes: meaning and troubleshooting Error code Error message Trouble shooting Error 01 Error 02 Error 03 Error 04 Error 05 Error 06 Error 07 Error 08 Error 09 Error 10 Error 11 Knife holder - limit position exceed- ed. Limit position of sledge exceeded Chamber - Alarm temperature ex- ceeded Power fai[...]

77 Leica CM 3600 – Cryomacrotome 6.9 Chamber illumination Click on the symbol to switch on the illumination of the cryochamber. Another mouse click will switch the chamber illumination back off. Active icons will light up in yellow. 6.10 Section documentation Click on the ‘Section documentation’ symbol to open the corresponding window. The wi[...]

78 Instruction manual V 6.0 - 08/2006 6.10.1 Tree diagram Indication of all main studies or substudies in a tree diagram. For naviga- tion purposes, click on the preceding plus or minus sign. The different study types are identified by the preceding colored folder symbols: • Yellow folder symbol Main studies (several). • Green folder symbol Mai[...]

79 Leica CM 3600 – Cryomacrotome 6.10.2 Studies protocol By clicking on a main study in the tree diagram, the following indication will be displayed in the „Studies protocol“ area: The table is divided into the following columns: • No. Running number automatically allocated by the application. • Date Date when the main study was created. [...]

80 Instruction manual V 6.0 - 08/2006 When clicking on an existing substudy in the tree diagram the following indication will be displayed in the „studies protocol“ area: The table is divided up as follows: • No. Indication of the running number automatically allocated by the application, in the order of date of creation of the main study. ?[...]

81 Leica CM 3600 – Cryomacrotome • Comment Comments can be entered by the user • Knife holder position, Knife holder position at the time the section was made 6. Software[...]

82 Instruction manual V 6.0 - 08/2006 6.10.3 Event table If you click on a main or substudy in the tree diagram, the following indication will be displayed in the „Event table“: The table is divided into the following columns: • No. Indication of the running event number automatically allocated by the application. • Date/time Indication of [...]

83 Leica CM 3600 – Cryomacrotome 6.11 Photo mode Clicking on the photo symbol activates or deactivates the „Photo mode“ and moves the microtome sledge to the me- chanical limit stop. When active, the symbol will light up in green. The photo mode enables you to position the specimen or rather the microtome sledge exactly for photos to be taken[...]

84 Instruction manual V 6.0 - 08/2006 6.13 Remote inquiry Clicking on the „Remote inquiry“ symbol opens the dialog box ‘Safety check - initialization’. Carry out the required safety query and subsequently confirm each safety query by mouse click on the corresponding check box. Having confirmed all safety queries, click on OK . The pcAnywher[...]

85 Leica CM 3600 – Cryomacrotome To establish a connection proceed as follows: 1. Doubleclicking on Icon „CM3600“ establishes the connection. The „pcAnywhere window waiting“ window will open up. Click on CANCEL to stop the connection from being established. After the connection has been successfully established, the window „Remote Conne[...]

86 Instruction manual V 6.0 - 08/2006 6.14 Help Clicking on the „Help“ symbol starts Adobe Acrobat Reader ® and opens the manual in pdf format. 6.15 Saving Click on the „Saving“ symbol to save the section data. ‘Saving’ must be clicked on as a first step to enable section documenta- tion via the foot switch. For subsequent steps, refer[...]

87 Leica CM 3600 – Cryomacrotome 6.18 User log-in / log-out Clicking on the symbol logs off the user currently logged in. Click again to open the ‘Start-and-login’ window. For further information, refer to chapter 6.2. Logging off does not automatically stop or delete the actions and settings initiated in the application. 6.19 Quitting the ap[...]

88 Instruction manual V 6.0 - 08/2006 7. Operating the Leica CM3600 To ensure proper operation of the Leica CM3600 as well as use of the specimen stages and the specimen blocks themselves, we strongly recommend to attend a Leica application training. 7.1 Check list The checklist below provides an overview on how to successfully operate the Leica CM[...]

89 Leica CM 3600 – Cryomacrotome 7.2 Switching the instrument on Proceed as follows: 1. Switch on the mains switch. For further information, refer to chapter 5.3. The refrigeration and control unit is activated. 2. Switch on the socket panel switch. For further information, refer to chapter 5.3. 3. Switch on the PC and peripherals. For further in[...]

90 Instruction manual V 6.0 - 08/2006 7.5 Specimen stages This chapter provides instructions on how to insert the knives. There are three different types of specimen stages: • Special holder, orientable, with ball-and-socket joint, • Specimen stage, orientable, with ball-and-socket joint, • Specimen stage, non-orientable. 7. Operating the Lei[...]

91 Leica CM 3600 – Cryomacrotome When inserting the specimen stages, bear in mind the following: • Prior to sectioning, the specimen stage should have been stored inside the cryochamber for at least 24 hours, to make sure it has the correct sectioning temperature (ca. -20° C). • To mount the entire block (specimen block on specimen stage) on[...]

92 Instruction manual V 6.0 - 08/2006 7.5.1 Inserting the specimen stage, orientable, with ball-joint To insert the specimen stage, orientable, with ball-and-socket joint proceed as described below. The special holder, orientable, with ball joint is inserted the same way. Tools required: • Fork wrench (size 17). Proceed as follows: 1. Use the kne[...]

93 Leica CM 3600 – Cryomacrotome 7. Operating the Leica CM3600 1 21 31[...]

94 Instruction manual V 6.0 - 08/2006 7.5.2 Inserting the specimen stage, non-orientable Tools required: • Allen key (size 6). Proceed as follows: 1. Use the knee lever to move the sledge to the right-side reversal point. For further information, refer to chapters 3.4.2 and 6.4.9. 2. Comply with the safety instructions provided in chapter 7.5. 3.[...]

95 Leica CM 3600 – Cryomacrotome 7. Operating the Leica CM3600 12 4 6 4 3 5 5[...]

96 Instruction manual V 6.0 - 08/2006 7.6 Knife This chapter provides instructions on how to insert the knives. Two types of knives can be used in the CM3600: • Standard knives • Disposable blade holder with disposable blade Since there is a considerable risk of injury, please be sure to comply with the following safety instructions: Prior to a[...]

97 Leica CM 3600 – Cryomacrotome 7. Operating the Leica CM3600[...]

98 Instruction manual V 6.0 - 08/2006 7.6.1 Inserting standard knives To insert a standard knife, proceed as follows. Tools required: • Allen key (size 4). Proceed as follows: 1. Use the knee lever to move the sledge to the right-side reversal point. For further information, refer to chapters 3.2.2 and 6.4.9. 2. Comply with the safety instruction[...]

99 Leica CM 3600 – Cryomacrotome 7. Operating the Leica CM3600[...]

100 Instruction manual V 6.0 - 08/2006 7.6.2 Inserting the disposable blade holder with disposable blade To insert the disposable blade holder with disposable blade proceed as described below. Tools required: • Allen key (size 4). • Allen key (size 2.5). Proceed as follows: 1. Use the knee lever to move the sledge to the left-side reversal poin[...]

101 Leica CM 3600 – Cryomacrotome 7. Operating the Leica CM3600[...]

102 Instruction manual V 6.0 - 08/2006 7.6.3 Adjusting the knife angle Tools required: • Allen key (size 5). Proceed as follows: 1. Comply with the safety instructions provided in chapter 7.6. 2. Loosen screws ( 1 ). 3. Rotate knife holder ( 2 ) selecting the desired knife angle on scale ( 3 ) (recommended angle = 20°). 4. Tighten screws ( 1 ). [...]

103 Leica CM 3600 – Cryomacrotome 7.7 Setting the trimming parameters Proceed as follows: 1. On the computer screen, open the ‘Main window’. Select the desired section thickness, sledge speed, cutting window and sectioning program. For further information, refer to chapter 6.4. For reasons of safety and accuracy, the maximum trimming thicknes[...]

104 Instruction manual V 6.0 - 08/2006 7.8 Setting the sectioning parameters Proceed as follows: 1. On the computer screen, open the „Main window“, select „ MANUAL “ mode of operation. Subsequently, select the desired section thickness and the cutting window. For further information, refer to chapter 6.4. 7.8.1 Sectioning the specimen For t[...]

105 Leica CM 3600 – Cryomacrotome Proceed as follows: 1. Select manual mode of operation and the desired section thickness. 2. Activate the section documentation function. 3. Use the knee lever to move the sledge to the right-side reversal point (left edge of the specimen block just about clears the knife). 4. Cut the section and clean the specim[...]

106 Instruction manual V 6.0 - 08/2006 16. Attach the section to a dehydration frame which has already been kept in the cryochamber for a while. 17. To speed up the dehydration process once you are done with the sectioning work, the automatic or manual dehydration function of the instrument can be activated. 7.9 Dehydrating the sections To be able [...]

107 Leica CM 3600 – Cryomacrotome 7. Operating the Leica CM3600[...]

108 Instruction manual V 6.0 - 08/2006 8.1 Cleaning For cleaning and disinfection of the stainless steel cryochamber and the microtome, use commercial alcoholic disinfectants or alcohol. The outer surfaces of the cryochamber and the control unit can be cleaned with mild household cleaners or with alcohol-based disinfectants or pure alcohol. Do not [...]

109 Leica CM 3600 – Cryomacrotome 2. Switch the instrument off and unplug from mains, see chapter 5.3.1. 3. Extract the knife from the knife holder, see chapter 7.6. 4. Lock the window, see chapter 3.4.3. 5 Open the cabinet lid, see chapter 3.4.4. 6. Remove screws ( 1 ) and screws ( 2 ). When removing screws ( 1 ), make sure the spacer rings ( 3 [...]

110 Instruction manual V 6.0 - 08/2006 8.2 Maintenance 8.2.1 Oiling Be sure to check the oil level every and before each section- ing procedure. Checking the oil level Unscrew both lids ( 1 ). The oil level must always be above the small capillary tubes ( 2 ). Filling up the oil reservoirs Unscrew both lids ( 1 ) and add low temperature oil PDP 40 [...]

111 Leica CM 3600 – Cryomacrotome 8.2.2 Defrosting Three different defrost procedures can be selected: • Automatic defrost I • Automatic defrost II • Manual defrost Automatic defrost I This defrost cycle takes place automatically once every 24 hours. The defrost cycle itself is mandatory, i.e. cannot be deactivated by the user, while the st[...]

112 Instruction manual V 6.0 - 08/2006 9.1 Extraction system The Leica CM3600 can be optionally equipped with an extraction device. This device greatly reduces the buildup of cutting waste in the cryocham- ber and microtome and simplifies cleaning of the instrument. To operate the extraction device, access to compressed air of at least 5 bar is req[...]

113 Leica CM 3600 – Cryomacrotome Function The extraction device is mounted sideways to the knife holder ( 6 ). Extraction is achieved by a nozzle system located in the housing ( 5 ), which is powered by compressed air (5 bar). To facilitate optimal removal of cutting waste, the swivel-mounted extrac- tion attachment ( 4 ) must be located directl[...]

114 Instruction manual V 6.0 - 08/2006 9.1.2 Exchanging the disposable bag The cutting waste collection system (similar to a household vacuum cleaner) consists of a disposable paper bag, protected by a sealable cloth bag. Both bags are attached to the extraction tube. Proceed as follows: 1. Press together the ends of the locking ring ( 1 ) and remo[...]

115 Leica CM 3600 – Cryomacrotome 9.2 Ordering information Specimen stages Specimen stage, non-orientable, 40 x 80 mm 0022 50103 Specimen stage, non-orientable, 80 x 180 mm 0022 50105 Specimen stage, orientable, with ball-and-socket joint 120 x 200 mm 0022 50106 Specimen stage, orientable, with ball-and-socket joint 120 x 250 mm 0417 19635 Specim[...]

116 Instruction manual V 6.0 - 08/2006 Positioning tool Positioning tool for disposable blades 0417 28760 Positioning tool for steel or Tungsten Carbide knives 0417 19141 Microtome knives Knife, facet angle 20°, 160 mm long, steel 7022 50143 Knife, facet angle 35°, 160 mm long, steel 0022 50144 Tungsten carbide knife, facet angle 35°, 160 mm lon[...]

117 Leica CM 3600 – Cryomacrotome Section collecting tape Section collecting tape, 38 mm x 66 m, type 810 7022 50203 Section collecting tape, 76 mm x 66 m, type 810 7022 50206 Section collecting tape, 148 mm x 66 m, type 810 7022 50215 Section collecting tape, 110 mm x 66 m, type 4248 0391 24390 Section collecting tape, type 810, for autoradiogra[...]