Leica M205 FA manuale d’uso

Leica M165 FC Leica M205 F A User Manual[...]

Leica M-series User Manual 2 General Instructions Safety concept Before using your microscope for the first time, please read the "Safety concept" br ochure included with your instrument. It contains addi - tional information about handling and care . Use in clean rooms The Leica M series can be used in clean rooms without any problems .[...]

Leica M-series User Manual 3 Impor tant Safety Notes User Manual The individual modules of the Leica M stereo - microscopy series include an interactive CD-ROM with all relevant user manuals in 20 other languages. Keep it in a safe place, and readily accessible to the user . User manu - als and updates are also available for you to download and pri[...]

Leica M-series User Manual 4 Symbols U sed W arning of a danger • This symbol indicates especially impor - tant information that must be read and complied with. F ailure to comply can cause the following: Hazards to personnel ρ F unctional disturbances or damaged instru - ρ ments W arning of hazardous electrical voltage  This symbol indic[...]

Leica M-series User Manual 5 Safety Instruc tions Description The individual modules fulfill the highest requirements for obser vation and documenta - tion of Leica ster eomicroscopes of the M series . Intended use Refer to "Safety Conc ept" booklet ▶ Non-intended use Refer to "Safety Conc ept" booklet ▶ Never use M series [...]

Leica M-series User Manual 6 Safety Instruc tions (cont' d.) Repairs, service work Refer to "Safety Conc ept" booklet ▶ Only original Leica M icrosystems spare ρ parts may be used. Before opening the instruments, switch off ρ the power and unplug the pow er cable.  T ouching the live circuit can cause injury. T ranspor t Use [...]

Leica M-series User Manual 7 Safety Instruc tions (cont' d.) • Direct contact with eyepieces is a poten - tial transmission method for bacterial and viral infections of the eye. The risk can be kept to a minimum by using personal eyepieces for each individual or detachable eyecups. Light sources: Safety regula tions Prot ective measures o[...]

Leica M-series User Manual 8 Safety Instruc tions (cont' d.) Supply unit Always unplug the supply unit from the power supply: When installing and disassembling the ρ lamp housing Before opening the lamp housing ρ When replacing the high-pressure mercur y ρ lamp and other par ts, such as the heat- absorbing filter or the collector During ma[...]

Leica M-series User Manual 9 Con tents General Instructions 2 Impor tant Safety Notes 3 Symbols Used 4 Safety Instructions 5 The Leica M Series for F luorescenc e Congra tulations! 1 3 A Step T owards Infinity 1 4 The Electronics: Comf ort, Convenience and Saf ety 1 5 The Modular Design: Everything is Relative 1 6 Maximum Compatibility 1 7 On W e [...]

Leica M-series User Manual 10 Quick Start Guid e The F astest Route to Success 6 4 Overview of an M S eries Microscope 6 5 The Corr ect Interpupillar y Distance 6 6 Using the Eyepiec es 6 7 F ocusing 6 8 Adjusting the Resistance of the F ocus Drive 6 9 Changing Magnification (Zoom) 7 0 Ratchet Steps and Magnification L evels 7 1 Parfocality: More[...]

Leica M-series User Manual 11 Sy stem Illuminatio n Leica LED5000 MCI™ 10 6 Leica LED5000 RL 10 8 Leica EL6000 – About the Instrument 10 9 Leica EL 6000 – Operation 11 1 Leica EL 6000 – T roubleshooting 11 2 Cleaning and Maintenance 11 3 Fluoresc enc e Filt er Changer 11 5 FIM – Fluoresc ence Intensity Manager 11 6 About Fluoresc ence Mic[...]

Leica M-series User Manual 12 T he L eica M S eries f or F luoresc ence[...]

Leica M-series User Manual 13 Congr atula tions! Congra tulations on obtaining your new Leica M series stereomicroscope . W e are convinced it will exceed your expectations, as never before have we applied our decades of experience in the areas of optics, mechanical engineering and ergonomics in such an uncompr omising manner . The Leica M series e[...]

Leica M-series User Manual 14 A Step T owards Infinity Ever since their introduction by Horatio S. Greenough, stereomicroscopes have worked according to the optical principles based primarily on Ernst Abbe's research. For over a century, ingenious optics designers and engi - neers hav e w orked t o push magnification, r eso - lution and imag[...]

Leica M-series User Manual 15 The Electronics: Comf ort, Con v enience and Safety for Y our Experiments Never before have electronics been used as extensively in a L eica series as in the new M series lineup. Optics carrier , stand, base and illuminator are all connected using electrical contacts—which provides a number of advan - tages. Reliabil[...]

Leica M-series User Manual 16 Have a special r equest? Let us know! Leica Microsystems enjo ys an exceptional repu - tation when it comes to devising customer- specific solutions. If you have a special request that cannot be met with standard parts, c ontact your Leica consultant . We have a solution for every problem. The Modular Design: E veryth[...]

Leica M-series User Manual 17 Leica engineers were careful to ensure that the new Leica M series—like its predecessors— remains compatible with existing series. T his means that objec tives, bases, tubes and so on can be reused. Objectives All new objec tives of the Leica M series are par focal, meaning that when used with the objective nosepie[...]

Leica M-series User Manual 18 On W e Go If your new Leica microscope has already been assembled and commissioned by your Leica consultant, click her e to skip through the instal - lation instructions and go directly to the Quick Start Guide on Page 63 . If, on the other hand, you are assembling the microscope yourself , continue with the " Ass[...]

Leica M-series User Manual 19 Assembly[...]

Leica M-series User Manual 20 The first step is to c onnect the focusing column of the M series to the corresponding base . T ools used Hex socket screw driver , 3 mm ρ Assembling the column adapter 1. Securely install the column adapter on the column using the four included screw s. Assembling the focusing column 2. Securely screw the focusing c[...]

Leica M-series User Manual 21 Assembling the F oc using Column with an Incident-ligh t Base When using an incident-light base, the focus - ing column and motorized focus are installed directly on the base; no extension plate is required . T ools used Hex socket screw driver , 3 mm ρ Assembly 1. Place the f ocusing column on the side. 2. Insert the[...]

Leica M-series User Manual 22  Depending on the work situation, it is useful to restrict the maximum trav el path of the stereomicroscope. This prevents injury during handling of the sample caused by fingers or hands becoming trapped or the specimen touching the objective or even being damaged by it. Readjusting the motorized focus The motorize[...]

Leica M-series User Manual 23 T ools used Hex socket screw driver , 4 mm ρ Assembling the optics carrier 1. Place the optics carrier on the focusing column so that the screw fits into the thread provided and the lug fits int o the groov e. 2. Pr ess the optics carrier backwards to the focusing column and scr ew it in place using your other hand.[...]

Leica M-series User Manual 24 T ube All intermediate tubes that fit between the optics carrier and the binocular tube are fitted in the same manner . T ools used No tools requir ed. ρ Prepara tions 1. Unscrew the positioning screw and r emove the protective co ver . Assembling the tube 2. Push the tube (for example , the inclined binocular tube)[...]

Leica M-series User Manual 25 Ey epieces T ools used No tools requir ed. ρ Magnification range Y ou can ex tend the overall mag nification range using available 10×, 16×, 25× and 40× wide - field eyepieces for persons w earing glasses. Prepara tion 1. If you want to use an optional gra ticule, insert it now (Page 79). 2. Remove the plastic [...]

Leica M-series User Manual 26 Objective T ools used No tools requir ed. ρ Prepara tion 1. Remove the protective cap on the optics carrier by turning it. Attaching the objective • Hold the objective firmly during assem - bly and disassembly so that it does not fall onto the stage plate. This applies par ticu - larly to the 2x planapochromatic[...]

Leica M-series User Manual 27 UV Prot ection S creen: Assembly T ools used Allen key ρ Intended use The UV protection screen in front of the speci - men plane prevents the user from looking directly into the UV rays . Safety Notes • UV radiation can damage the eyes. Theref ore, it is mandatory to install the UV filter and adjust it correctly[...]

Leica M-series User Manual 28 Prepara tions • Hold the objectives firmly during assembly and disassembly so that they do not fall onto the stage plate . Move the drive housing all the way upwards ρ and remove the optics carrier , if the carrier has already been installed. Assembly 1. Remove the transport anchor from the objective nosepiece. [...]

Leica M-series User Manual 29 Objective Nosepiece – A djusting Parfocality The following procedure only has to be carried out once. Afterwards, both objectives are par fo - cal, meaning that the specimen remains in focus when the objective nosepiece is rotated . This procedure must be repeated if you replace either of the two objectives with anot[...]

Leica M-series User Manual 30 T ransmitted-light Base TL ST Unpacking the base The base is deliver ed with the adapter plat e installed. Make sure the instruments are unpacked on a flat , sufficiently dimensioned, and non-slip sur face. Focusing driv e and column 1. Unscrew the ex tension plate from the base using the Allen key provided . 2. Attac[...]

Leica M-series User Manual 31 FluoC ombi III™ – Preparations  Before assembling the FluoCombi™, you have to disconnect all instruments from the pow er supply . F ailure to obser ve these instructions can result in damage to the micro - scope and the connected instruments. Prepara tions 1. Remove the two screws used as transport anchors. 2.[...]

Leica M-series User Manual 32 FluoC ombi III™ – Assembly 1. Guide the FluoC ombi III™ in from below , while holding it flat. Ensure that it is aligned with the holder on the focusing column at the left and right. 2. Hold the FluoC ombi III™ securely and screw it to the focusing column using the provided scr ew .[...]

Leica M-series User Manual 33 Assembling the optics carrier 1. Unscrew the three screws and the adapter ring on the optics carrier . 2. Attach the optics carrier to the FluoCombi III and screw it into plac e. 3. Open the iris diaphragm complet ely . 4. Assemble the tube (see P age 24). 5. Assemble the illuminator (see P age 51 ff.) FluoC ombi III?[...]

Leica M-series User Manual 34 FluoC ombi III™ – Adjusting the Parfocality Setting up the objective nosepiece  The following configuration only has to be carried out once. It ensures that the par focality remains intact, and thus the speci - men does not need to be brought into focus again. 1. Place a specimen under the micr oscope. 2. Rotat[...]

Leica M-series User Manual 35 FluoC ombi III™ – Adjusting the Parfocality (cont'd .) 9. By turning the objective ring, focus on the specimen un til the specimen appears abso - lutely sharp . 10. Enlarge the view just enough for the cen ter of the specimen to be visible in the field of view . 11. Refocus. 12. Tighten the clamping scr ew (s[...]

Leica M-series User Manual 36 4. Cent er the objec tive over the specimen by loosening or tightening the screw on one side of the objective holder while turning the screw on the other side in the opposite direction. FluoC ombi III™ – Centering the Objectives  The following configuration only has to be carried out once. It ensures that the s[...]

Leica M-series User Manual 37 FluoC ombi III™ – Filter and UV Pr otection S creen Inserting the dichroic mirror 1. Pull the dummy filter carrier out of the holder . 2. Insert the corresponding dichroic mirror . Assembling the UV protection screen 1. Screw the UV prot ection screen to the holder provided on the optics carrier . 2. Orient the UV[...]

Leica M-series User Manual 38 Removing the transport anchors • Before you can use the transmitted- light base for the first time, it is abso - lutely necessar y to remove the two transpor t anchors. T ransmitted-light Base TL BFDF: Befor e First U se Anchor of switching slide Anchor of mirror[...]

Leica M-series User Manual 39 T ransmitted-light Base TL BFDF Standard delivery The base is deliver ed with the adapter plat e installed. The selected stage (Leica IsoPro™ mechanical stage or Leica standard stage 10 447 269), and the focusing drive will have to be mounted later . Stage assembly The Leica TL BFDF transmitted-light base can be equi[...]

Leica M-series User Manual 40 The base is deliver ed with the adapter plat e installed. The selected stage (Leica IsoPro™ mechanical stage or Leica standard stage 10 447 269), and the focusing drive will have to be mounted later . Ensure that the instruments are unpacked on a level, adequa tely sized, and nonskid underlay . Stage assembly The Lei[...]

Leica M-series User Manual 41 IsoPro™ Manual Mechanical Stage: Assembly Before the Leica IsoP ro™ mechanical stage is mounted to the base, the axis containing the control buttons is attached either on the lef t or the right side of the mechanical stage. Leica IsoPro™ Mechanical Stage Before the Leica IsoP ro™ mechanical stage is mounted to [...]

Leica M-series User Manual 42 Leica IsoPro™ Manual Mechanical Stage: Assembly (con t' d.) 1. T ake the glass pla te from the mechanical stage and turn it around. 2. Change the gear rod from the left to the right-hand side. Control assembly 1. T ake the glass pla te from the mechanical stage and turn it around. 2. Attach the axis with the con[...]

Leica M-series User Manual 43 Leica IsoPro™ Manual Mechanical Stage: Assembly (con t' d.) 3. Attach the mechanical stage evenly to the three threaded holes . 4. Now move the mechanical stage as far as it will go in the direction of the column. 5. Insert the glass plate into the mechanical stage. Focusing driv e and column 1. Unscrew the ex t[...]

Leica M-series User Manual 44 Basics The transmitted light bases of the Leica TL series ( TL BFDF , TL RC™, TL RCI™) are supplied with an installed ex tension plate. The selected stage (Leica IsoPro™ mechanical stage or standard stage 10 447 269) and the focusing drive will have to be moun ted later . The motorized mechanical stage is a sensi[...]

Leica M-series User Manual 45 3. Remove the two M3 screws and the holder from the mechanical stage. 4. Remove the two M4 screws and the sleeve from the mechanical stage. 5. Remove the four shock-absorbing cartons from the mechanical stage.  After remo val, keep all the transport anchors in the plastic bag provided for future transport. Leica Iso[...]

Leica M-series User Manual 46 Leica IsoPro™ Mot orized Mechanical Stage: Assembly (cont' d.) The bases The TL BFDF , TL RC™ and RCI™ transmitted-light bases can be equipped with three different stages: Standard stage, manual and automated IsoPro™ mechanical stage . The selected stage is mounted on the base befor e commissioning . Y ou[...]

Leica M-series User Manual 47 Leica IsoPro™ Mot orized Mechanical Stage: Assembly (cont' d.) Mechanical stage for X- Y stage control module 1. Plug the CTL2 plug of the mechanical stage motor into one of the three available C TL2 inter faces. 2. Plug the CTL2 plug of the Leica PSC contr ol - ler into another CTL2 inter face. The third C TL2 [...]

Leica M-series User Manual 48 The new Leica M-series features extensive encoding with which various microscope data and settings can be read out, transferred to the PC and reproduc ed later . The inter face to the optics carrier is on the ρ column. The terminals The connection to the PC and to other instru - ments is made using the terminals on th[...]

Leica M-series User Manual 49 The integrated cable duc t in the column enables a neat cable layout around the microscope. F or example, the USB or FireW ire cables of the camera can be stow ed in the cable duct. Feeding the cables 1. Unscrew the three screws on the cable duct. 2. Remove the c over of the cable duct. 3. Place the cables in the cable[...]

Leica M-series User Manual 50 Cables: Diagram TL RCI™ DCI Controlbo x PSC SmartT ouch PC USB Cross Stage Smart Move Pow er/USB LED5000 RL LED5000 MCI™ Footswit ch(es) Please use the Leic a A pplication Suite to configur e the functions of the system. Alternative installa tion 115/230 V[...]

Leica M-series User Manual 51 The L eica LED5000 MCI™ (f or "Multi C ontrast Illumination") is installed using t wo screws. For optimum accessibility, the optics carrier should be remov ed during assembly (see Page 23.) Constrain ts The Leica LED5000 MCI™ cannot be used together with the objective nosepiece. Assembly • 1. Hold th[...]

Leica M-series User Manual 52 Under certain cir cumstances, the light source must not be moved along with the optics carrier . A typical example is multifocus images in which the Z-stack changes while the angle of incidence of the light must remain the same. F or such purposes, the Leica LED5000 MCI™ is directly fastened to the column. Installati[...]

Leica M-series User Manual 53 The ring illuminator , the Leica LED5000 RL ("Ring Light"), is installed on the objective using a single scr ew . It has been optimized for a work - ing distance between 60 and 70 mm. Constrain ts The Leica LED5000 RL can be used in conjunc - tion with the planapochromat 1× and planapo - chromat 0.63× objec[...]

Leica M-series User Manual 54 • The manufacturer assumes no respon - sibility or liabilit y for any use outside of the intended use or use outside of the specifica - tions fr om Leica M icrosystems Wetzlar GmbH or any risks resulting fr om such use. In such cases, the D eclaration of Conformity shall be invalid . • This (IVD) device is n[...]

Leica M-series User Manual 55 Leica EL6000 – Safety Notes (cont' d.) • The Leica EL6000 compac t light source generates high-energy light with invisi - ble componen ts. There is a risk of being dazzled or blinded by light! Never look directly into the light guide output of the instrument or into the output of the light guide connected t[...]

Leica M-series User Manual 56 Leica EL6000 – Safety Notes (cont' d.) Electrical safety F or indoor use only . Supply voltage 100–240 V~ (±10%) Fr equency 50–60 Hz Po wer consumption Max. 210 V A F uses 5×20, 2.5 A, slow-blowing , breaking capacity H, see Page 113 Ambient temperatur e 0 – 40 °C Relative humidity 10%–90% Overvoltage[...]

Leica M-series User Manual 57 Setting up the instrument • Set up the L eica EL6000 so that the fron t is readily accessible and visible . The ventilation slots on the sides and back ρ panel of the device must not be cov ered. Maintain a free room of at least 150 mm on ρ the rear panel of the instrument. Instructions for lamp replacement •?[...]

Leica M-series User Manual 58 Leica EL6000 – Prepar ation (c ont' d.) 4. Carefully lay the Leica EL6000 on its side so that the opening faces you . This makes it easier to insert the lamp. 5. Pull the pressure bolt back towards the front plat e using the lever . 6. Insert the lamp. Ensure that the gr oove in the con tact sur face of the lamp[...]

Leica M-series User Manual 59 Leica EL6000 – Connection to the Fluor escenc e Lamp Housing • Always connec t the light guide to the lamp housing of the stereomicroscope in order to prevent danger to the user from the high-energy light. 1. Unscrew the positioning screw and r emove the protective cover of the fluorescence lamp housing. 2. Ins[...]

Leica M-series User Manual 60 Leica EL6000 – Prepar ation (c ont' d.) 7. When the lamp makes full contact with the lamp mount, release the lev er . 8. Connect the plug of the lamp with the coupling in the instrument. Ensure that no wires are touching the reflector of the lamp. 9. Check to make cer tain that a heat-absorb - ing filter has b[...]

Leica M-series User Manual 61 Leica EL6000 – Replacing the Lamp The procedure for replacing the lamp does not differ from the installation procedure outlined on Page 57, ex cept for the fac t that the existing lamp has to be remov ed first. Replacing the lamp  The lamp used in the instrument becomes very hot during operation and has high int[...]

Leica M-series User Manual 62 Leica EL6000 – Connection to the Fluor escenc e Lamp Housing (cont' d.) 6. Insert the long end of the light guide into the light output of the Leica EL6000. There must be a noticeable click. • Only use a light guide with a light input of the t ype "S torz long" , as otherwise damage to the instrum[...]

Leica M-series User Manual 63 Q uick Star t Guide[...]

Leica M-series User Manual 64 The F astest Route t o Success Y our Leica stereomicrosc ope has been delivered in completely assembled condition by your Leica par tner , and naturally you want to get right to work. Ther efore , your next step should be to study the Q uick Star t Guide, which outlines the most important steps at a glance. This manual[...]

Leica M-series User Manual 65 Overview of an M Series M icroscope 1 Eyepieces for spectacle wearers with dioptric correction and eyecups 2 Trinocular tube 3 Fluorescence housing 4 Filter Changer 5 Display 6 Motorized Focus 7 Objective Nosepiece 8 Leica SmartTouch 9 UV protection screen 1 2 3 4 5 7 9 8 6[...]

Leica M-series User Manual 66 The C orrect Interpupillary D istance The interpupillary distance is correctly set if you see a single circular image field when look ing at a specimen. If y ou ar e still a novice microscope user , you may need a shor t time to become accustomed to this. Not to worry—after a little while, it will become automatic .[...]

Leica M-series User Manual 67 If you wear glasses: 1. Hold the eyepiece firmly and rotate the eyecups clockwise towards the rear , as otherwise the viewing distance is too great. 2. If an eyepiece is equipped with the inte - grated dioptric c orrection, turn the value to the "0" mark. By the way , one benefit of viewing with eyeglasses [...]

Leica M-series User Manual 68 F oc using F ocusing raises or lowers the stereomicroscope using the focusing drive. The specimen detail is brought into sharp focus as soon as it is in the focal point of the objective. The focusing drive can be operated either ρ left- or right-handed. Focusing The inner , coarse adjustment is used for ρ covering g [...]

Leica M-series User Manual 69 Adjusting the Resistanc e of the F oc us Drive Adjusting the resistance Is the focus movement too loose or too tight? Does the outfit tend to slide downwards? The resistance can be adjusted individually depend - ing on the equipment weight and personal prefer ences as follow s: 1. Grip the outer driv e knobs with both[...]

Leica M-series User Manual 70 Changing Magnification (Zoom) All M series microscopes have an integrated zoom. The name indicates the zoom range cover ed: Leica M165 C = 16.5:1 ρ Leica M205 C = 20.5:1 ρ The rotar y knob for the zoom can be used either left or right-handed. Zooming 1. Look int o the eyepieces . 2. F ocus on the specimen. 3. Rotate[...]

Leica M-series User Manual 71 The zoom butt on can optionally be operat ed either with or without ratchet steps. Continu - ous z oom is possible when the ratchet steps are disabled, which many users find convenient. On the other hand, when the ratchet steps are enabled, photographs , measurement results etc. can be repr oduced more ac curately . E[...]

Leica M-series User Manual 72 Parfocality: More Comf or t and Con venience for Y our W ork All Leica stereomicroscopes are parfocally matched, meaning that you can view a focused specimen from the lowest to the highest magnification without having to r efocus. The focus needs only be readjusted if you want to see a specimen location that is locate[...]

Leica M-series User Manual 73 The iris diaphragm in the optics carrier of your M series microscope fulfills the same purpose as the iris diaphragm in a camera: it regulates the av ailable light, which changes the depth of field. The "depth of field" (or "focus depth") is the area of a specimen that is brought into sharp focus[...]

Leica M-series User Manual 74 E y epieces[...]

Leica M-series User Manual 75 Magnification F ac tors of the Ey epieces An eyepiece not only makes it possible to look passively into the micro - scope, but also has a critical effect on the maximum magnification. The magnification factor is between 10x and 40x. The following e yepieces ar e available f or the M series: Magnification Dioptric [...]

Leica M-series User Manual 76 Health Notes Pot ential sources of infection • Direct contact with eyepieces is a potential transmission method for bacterial and viral infections of the ey e. The risk can be kept to a minimum by using individual eyepieces or detachable eyecups. Eyecups can be ordered separately . Please contact your Leica partne[...]

Leica M-series User Manual 77 All Leica eyepiec es are also available with built- in dioptric correction, allowing the microscope to be used without glasses even by those with vision problems. The correction comprises ±5 diopter settings. Using the Dioptric Corr ection 1. Set the dioptric correction of both eyepieces to the mid position ("0&q[...]

Leica M-series User Manual 78 Adjusting 1. Set the dioptric c orrection for both eyepieces to "0" . 2. Select the lowest magnification and focus on a flat specimen. 3. Select the highest magnification and read - just the sharpness. 4. Select the lowest magnification again, but do not look into the eyepiec es. 6. Rotate the eyepieces c[...]

Leica M-series User Manual 79 Graticules 2. Clamp the graticule on the insert, applying moderate pressure. Ensure that the gr ati - cule fits tightly . 3. Screw the insert and graticule firmly in to place and replace the ey epiece in the tube. 4. Y ou can now align the graticule by rotating the eyepiece in the tube and then tighten - ing it using[...]

Leica M-series User Manual 80 Photogr aph y & V ideo[...]

Leica M-series User Manual 81 F or most microscope users, digital documen - tation has become an invaluable par t of their work. Research results can be presented in an attractive manner; measur ements on the digital image provide clarity and, in conjunc tion with the motorized IsoPro™ mechanical stage, even images of large specimens can be captu[...]

Leica M-series User Manual 82 Photo T ubes and C-mounts Application All Leica DFC cameras are equipped with a standardized C-mount inter face. In turn, the C-mount adapter for the respective trinocular tube is connected t o this int er face. This adapter creates a solid mechanical c onnection between the microscope and camera and ensures opti - mum[...]

Leica M-series User Manual 83 T rinoc ular Video/Phototube 50% Use With its third beam path, the trinocular video/ phototube 50% enables you to simultaneously view and photograph a specimen. The av ailable light is divided as follows: 50% is available for the two ey epieces. ρ 50% of the light is diver ted to the video/ ρ photo beam path. Assembl[...]

Leica M-series User Manual 84 Use With its third beam path, the trinocular video/ phototube 100% enables you to either view or photograph a specimen. This means that 100% of the light is available to one or the other beam path. The other beam path remains opaque or black. Assembly F asten the "trinocular tube 100%" to the optics carrier i[...]

Leica M-series User Manual 85 Microsc ope Carrier[...]

Leica M-series User Manual 86 Use The objec tive nosepiece enables you to switch between two objectives using just one hand movement , f or example bet ween a 1× and a 2× planapochromat. Parfocal work With the new M series objectives, the parfo - cality is maintained even when objec tives are changed, meaning that the specimen remains in sharp fo[...]

Leica M-series User Manual 87 Objec tiv es and Optic al A c c essories[...]

Leica M-series User Manual 88 The Differ ent T ypes of Objec tives T o meet the various requir ements regarding imaging properties, there is a choice of high- quality interchangeable planachromatic and planapochromatic objectives and also lower- priced inter changeable achromatic objectives. Achroma tic objectives are particularly ρ suited for sp[...]

Leica M-series User Manual 89 Bases[...]

Leica M-series User Manual 90 Leica TL ST T ransmitted-light Base: C ontrols 1 Adapter plate for easy assembly of focusing drives 2 Removable glass plat e 3 Controller for light int ensity 4 Adjustment for deflection mirror Rear side of the transmitted-light base TL ST 1 Screws for changing the halogen lamp 2 Pow er connection socket 3 Pow er swit[...]

Leica M-series User Manual 91 Leica TL ST T ransmitted-light Base: Operation Light intensity contr ol The left control adjusts the intensity of the 12 V/20 W halogen illumination. 1. Switch on the illumination of the base at the power switch. 2. F ocus on the specimen. 3. Set the illumination to the desired intensity using the left control. T ransm[...]

Leica M-series User Manual 92 Changing the halogen lamp  Before you change the lamp, it is abso - lutely necessary to unplug the power plug from the base to prev ent the risk of electric shock!  The halogen lamp becomes ver y hot during operation. Therefore , to avoid being burned, let the base cool off for approx. 10 minutes after switching[...]

Leica M-series User Manual 93 Leica TL BFDF T ransmitted-light Base: C ontrols 1 Adapter plate for easy assembly of focusing drives 2 Standard stage 10 447 269 3 Button to toggle between bright field and dark field 1 2 3 Extension plate of the T ransmitted-light base TL BFDF Adapter at the focusing drive Button to toggle between bright field and[...]

Leica M-series User Manual 94 Light intensity contr ol • Observe the user manual—in par ticu - lar , all safety regulations—from the manufacturers of the light guide and cold light source . Switch on the cold light source ac cording to ρ the manufac turer's user manual and adjust the brightness. T ransmitted-light control The Leica T[...]

Leica M-series User Manual 95 Leica TL RC™ / TL RCI™: Con trols V ertical column - transmitted-light base adapter plate 1 Heat sink of the integrated halogen illumina - tion (only TL RCI™) 2 Extension plate for focusing drives 3 Standard stage 10 447 269 4 Filter holder 5 Control of top and bottom flaps of the Rottermann Contrast™ 6 Button[...]

Leica M-series User Manual 96 Leica TL RCI™: The Deflection Mirror Inv erted world? Depending on the properties of the specimen (refractive index of the environment) and the perception of the viewer , it is possible that the switches described below for positive and inverted relief contrast are to be operated in r everse. This means that the low[...]

Leica M-series User Manual 97 Leica TL RCI™: Color Intensity and T emperature 1 Potentiometer for controlling the CCIC™ (Constant Color Intensity Control) illumina - tion intensity 2 Potentiometer for controlling the color temperature The transmitted-light base TL RCI™ has two electronic potentiometers that control the color intensity (1) and[...]

Leica M-series User Manual 98 Setting the relief contrast The two switches on the left side of the trans - mitted-light base TL RC™/TL RCI™ activate two built-in flaps. The outer switch (1) controls the inverted relief contrast, and the inner switch (2) adjusts the positive relief c ontrast. Depending on the flap position, a par t of the open[...]

Leica M-series User Manual 99 Leica TL RCI™: Methods in T ransmitted Light V ertical bright-field illumination Suitable for stained amplitude specimens with sufficient contrast. The light beams are deflected ver tically through the specimen. This results in an accurate bright field with maximum brightness. Inclined transmitted light Suitable f[...]

Leica M-series User Manual 100 Leica TL RCI™: Relief Images Starting position 1. Push the deflection mirror all the way back towar ds the column. 2. T urn the deflec tion mirr or into the notch position at an angle of 45°. Positiv e relief contr ast Suitable for semitransparent and transparent specimens. The phase structures look like hills. T[...]

Leica M-series User Manual 101 Dynamic relief contrast Suitable for semitransparent and transparent specimens. By tilting the deflection mirror slightly , you can move the gaps over the entire field of view and quickly toggle bet ween positive and negative relief images. The dynamic effec t makes it easy to distinguish phase struc tures from amp[...]

Leica M-series User Manual 102 Filters f or Leica TL RC™ and TL RCI™ The transmitt ed-light bases TL RC™ and TL RCI™ can be equipped with up to three filters— available as accessories—simultaneously . By customer request, the filters are also available as one- off items. 1. Switch off the light source or click (Leica TL RCI™) the [...]

Leica M-series User Manual 103 Leica IsoPro™ (Non-mot orized): C ontrols Operating the Leica IsoPro™ mechanical stage 1. T o move the stage in the X direction, rotate the outer knob. 2. T o move the stage in the Y direction, rotate the inner control ring . 1 2[...]

Leica M-series User Manual 104 Leica IsoPro™ (Mot orized): C ontrols 1 Leica IsoPro ™ motorized mechanical stage 2 Housing with motorization 1 Sub-D interface for Leica SmartMove™ 2 3 CTL2 interfaces 3 USB interface (type B) 4 Socket for grounded power cable 1 Quick control/memory function 2 Fine control in X direction 3 Fine control in Y dir[...]

Leica M-series User Manual 105 S y st em Illumina tion[...]

Leica M-series User Manual 106 Use The L eica LED5000 MCI™ (f or "Multi C ontrast Illumination") is a universal high-output illu - minator . Three groups of 3 LEDs each can be arranged as desired around the specimen. The targeted contr ol of the LEDs enables various illumination scenarios to be implement ed. Prepara tion Hold the MCI wi[...]

Leica M-series User Manual 107 Using the ke yboard Use the ρ key to switch the illumination on or off . Use the "+" and "–" keys to adjust the ρ brightness in 10 increments . T ouch either of the two butt ons to adjust the intensity in small increments. Hold a key to change the intensity more quickly . The illumination scen[...]

Leica M-series User Manual 108 Leica LED5000 RL Use The Leica LED5000 RL ("Ring Light") generates a ver y bright and homogenous incident light. It has a diameter of 80 mm and illuminates the specimen with 48 LEDs that can be switched on and off completely or in various combinations. It is controlled using either the integra ted or via th[...]

Leica M-series User Manual 109 The Leica EL6000 compac t light source is intended for biological routine and research work, including the examination of specimens from the human body for the purpose of gain - ing information. • The Leica EL6000 compact light source is intended exclusively for operating mercury shor t-arc lamps of type HXP-R120[...]

Leica M-series User Manual 110 Leica EL6000 – About the Instrument (c ont' d.) Compatible ligh t guides The Leica EL6000 compac t light source is compatible with liquid light guides that are compatible with a "Storz long" light inlet and whose transmission cur ve fits the lamp used. Where applicable, ref er t o the lamp's dat[...]

Leica M-series User Manual 111 Leica EL 6000 – Operation Controls Running-time meter The running-time meter measures the burn - ing time of the lamp, not the switch-on time of the Leica EL6000 compact light source. The running-time meter displays its current status in this format: hours – minutes . 1. Pr ess the reset button to set the running-[...]

Leica M-series User Manual 112 Leica EL 6000 – T roubleshooting F ault description Possible faults Remedy Po wer indicator is not illumina ted after being switched on, instrument shows no function No power supply v oltage present; connecting cable not plugged in correctly/defective Check power supply v oltage and connecting cable F uses in instru[...]

Leica M-series User Manual 113 Cleaning and Maintenanc e • Before carr ying out cleaning tasks, switch off the Leica EL6000 compact light source . Use only a dry, lint-free cloth for cleaning. When cleaning, ensure that no dust or other foreign bodies enter the device thr ough the ven tilation slots. In case of damage (even external damage), [...]

Leica M-series User Manual 114 F luoresc enc e[...]

Leica M-series User Manual 115 Filt er Changer The patented FL UOIII® filter system consists of a filter changer for barrier and excitation filters, a UV shutter that can be activated/deactivated, and a filter inser t for a filter holder with your choice of filter . The filter changer can hold a total of 4 filter sets. The filter sets for[...]

Leica M-series User Manual 116 FIM – Fluor escence In tensity Manager FIM consists of a diaphragm disk with pinhole diaphragms of varying transmission capaci - ties. The intensity of the excitation light can be reduced in five fixed steps. The light can be changed from 100% to 55%, 30% and 17% down to 10%. The advantage of this procedure is its[...]

Leica M-series User Manual 117 About Fluor escenc e Microscop y Functional principle Fluoresc ent substances light up when irradi - ated with shor t-wave excitation light, and fluo - rescence microscop y takes advantage of this property. Specimens without self-fluorescence are stained with a fluorescent substance. One such dye is green fluoresc[...]

Leica M-series User Manual 118 Rapid Filter Changers and F ilter T ypes Filter sets Excitation filter Barrier filter Designation GFP 425/60 nm (395–455 nm) 480 LP GFP1 GFP Plus 480/40 nm (460–500 nm) 510 LP GFP2 GFP plants 470/40 nm (450–490 nm) 525/50 nm (500–550 nm) GFP3 UV 360/40 nm (340–380 nm) 420 LP UV Violet 425/40 nm (405–445 [...]

Leica M-series User Manual 119 Simple filter holders Simple filter holders Three simple filter holders are included with the L eica M165 FC and the L eica M205 F A. These filter holders have t wo empty openings for the observation beam paths. The third opening, to the illumination beam path, is closed. Use this filter holder if you want to w o[...]

Leica M-series User Manual 120 Equipping the F ilter Changer • When inser ting the filter sets, av oid touching the filters in order to avoid fingerprints. Clean dir ty filters immediately using a soft, lint-free cloth and pure alc ohol. Removing the simple filter holder The Leica M165 FC and the Leica M205 F A are equipped at the fac tor[...]

Leica M-series User Manual 121 Obser vation without F luorescence  W e recommend that you first familiarize yourself with the contr ols of your Leica fluorescence microscope in transmitted light without the presence of fluorescence illumina - tion. • After switching on the high-pressure mercury burner , you must adjust the arc lamp immed[...]

Leica M-series User Manual 122 Double Iris Aperture The built-in double -iris diaphragm regulates the depth of field. Note that: The greater the depth of field, the darker ρ the image becomes. In the same way , the exposure time ρ becomes longer for photog raphs. The resolution decr eases. ρ Adjusting the double-iris diaphragm using the knurle[...]

Leica M-series User Manual 123 Commissioning the F luorescence S yst em After switching on the high-pressure mercury burner , you must adjust the ar c lamp imme - diately (see User Manual M2-216-1). Do not switch on the supply unit until you feel confi - dent using the Leica ster eomicroscope . • F ollow all safety instruc tions when handling[...]

Leica M-series User Manual 124 A cc essories[...]

Leica M-series User Manual 125 With its non-slip controllers and k nobs, the Leica Smar tMove control unit offers the right feel for handling motoriz ed Leica instruments . Controls The functions of the individual controls can be configured both directly on the L eica Smart - Move and using the L eica Application Suite. F or a detailed descriptio[...]

Leica M-series User Manual 126 With the touchscreen of the Leica Smar tT ouch™, you can control your experiment procedur es with a few hand mov ements and never lose sight of all your important optical parameters. The most important c ontrol functions on the control unit can be adjusted to y our specific needs with freely programmable rotary k n[...]

Leica M-series User Manual 127 Leica PSC Con troller Use The Leica PSC controller gives you precision control of the Leica IsoPr o™ mot orized mechan - ical stage. Connection Connect the Leica PSC controller and the Leica IsoPro™ motorized mechanical stage to the DCI box. Passing ov er the specimen quick ly Move the joystick in any desired dire[...]

Leica M-series User Manual 128 Dimensional D ra wings[...]

Leica M-series User Manual 129 Leica M165 FC Leica M165 FC with incident light base and new focusing c olumn (dimensions in mm) 385 154 340 390 440 95 154 116 431 547 292 217 385 154 340 390 440 95 154 116 431 547 292 217 385 154 340 390 440 95 154 116 431 547 292 217[...]

Leica M-series User Manual 130 Leica M165 FC (cont' d.) Leica M165 C with TL RCI™ transmitted light stand , Leica IsoPro™ manual mechanical stage and manual focusing drive 497 292 217 187 154 max. 370 X = +-75 mm 370 95 300 249 Y = +-50 mm 116 431 547 497 292 217 187 154 max. 370 X = +-75 mm 370 95 300 249 Y = +-50 mm 116 431 547 497 292 2[...]

Leica M-series User Manual 131 Leica M165 FC (cont' d.) Leica M165 C with TL RCI™ transmitted light stand and manual f ocus 340 430 85 154 356 154 292 217 106 431 537 340 430 85 154 356 154 292 217 106 431 537 340 430 85 154 356 154 292 217 106 431 537[...]

Leica M-series User Manual 132 Leica M205 F A Leica M205 F A with TL BFDF tr ansmitted light stand, Ergo Trinoc tube , DFC camera and motorized f ocusing drive 370 154 340 390 90 154 292 116 431 547 370 154 340 390 90 154 292 116 431 547 370 154 340 390 90 154 292 116 431 547[...]

Leica M-series User Manual 133 Leica M205 F A (cont' d.) Leica M205 F A with TL RCI™ tr ansmitted light stand, Ergo Trinoc tube , DFC camera and motorized f ocusing drive 385 154 340 390 440 95 154 116 431 547 292 385 154 340 390 440 95 154 116 431 547 292 385 154 340 390 440 95 154 116 431 547 292[...]

Leica M-series User Manual 134 Leica M205 F A (cont' d.) Dimensions of Leica M205 F A with TL RCI™ transmitt ed light stand, IsoPr o™ mechanical stage, Ergo T rinoc tube, DFC camera and motoriz ed focusing drive 497 187 154 max. 410 X = +-75 mm 370 95 300 249 Y = +-50 mm 292 116 431 547 497 187 154 max. 410 X = +-75 mm 370 95 300 249 Y = +[...]

Leica M-series User Manual 135 Leica LED5000 MCI 132 275.3 157 83.5 112 45 112 90 45 36 22 132 275.3 157 83.5 112 45 112 90 45 36 22 132 275.3 157 83.5 112 45 112 90 45 36 22 Leica LED5000 MCI (dimensions in mm)[...]

Leica M-series User Manual 136 Leica LED5000 RL 120 ø70 30 153 153 ø80[...]

Leica M-series User Manual 137 MA TS TL 190 140 220 4 11 134 98 170 10 447 164 10 447 275[...]

Leica M-series User Manual 138 Specific a tions[...]

Leica M-series User Manual 139 Leica M165 FC / Leica M205 F A Leica M165 FC Leica M205 F A Optical data Zoom 16.5 : 1 manual 20.5 : 1 automated with F usionOptics™ Data with standard optics (1×objec - tive/10× eyepiec es) – Zoom range – Resolution – W orking distance – Object field 7.3×–120× max. 453 lp/mm 61.5 mm (planapochromat) [...]

Leica M-series User Manual 140 Leica M165 FC / Leica M205 F A (cont’ d.) Leica M165 FC Leica M205 F A Optics carrier 100% apochromatic optical syst em CMO (Common Main Objective) lead-fr ee CMO (Common Main Objective) lead-fr ee Specific sur face resistivity (housing) 2×1011 Ω/mm2 discharge time <2 seconds fr om 1000V to 100V 2×1011 Ω/m[...]

Leica M-series User Manual 141 Leica M165 FC / Leica M205 F A (cont’ d.) Leica M165 FC Leica M205 F A Additional objectives (non-parfocal) 1× planachromat, 0.8× planapochr omat, 0.63× planapochromat, 0.5× planapochr omat 1× planachromat, 0.8× planapochr omat, 0.63× planapochromat, 0.5× planapochr omat Interpupillary distance 50 mm–105 m[...]

Leica M-series User Manual 142 Leica M165 FC / Leica M205 F A (cont’ d.) Leica M165 FC Leica M205 F A Incident light stand Large incident light base with black/white stage pla te and Antishock™ feet Stages Gliding stage, MA TS heating stage, cup stage , rotatable polariza tion stage, Leica IsoP ro™ mechanical stage (manual and motorized) Illu[...]

Leica M-series User Manual 143 Leica EL6000 – Specifications Dimensions ( W×H×D) Width×height×depth 130 mm × 210 mm × 290 mm Environmen tal conditions Permitt ed ambient temperatur e -20 t o 85 °C Permitt ed relativ e humidity 10% to 90%, non-condensing Operation Permitt ed ambient temperatur e 0 to 40 °C Permitt ed relativ e humidity 10[...]

Leica M-series User Manual 144 Leica EL6000 – Specifications (cont' d.) Light output Design T ype: Storz, long Special feature Self-lock ing if no light guide is plugged in Fa n Switch-on delay Approx. 60 s after lighting the lamp Interface T ype Remote control input for internal shutter Design 9-pin D-Sub socket Remote signal level – Shu[...]

Leica M-series User Manual 145 Leica TL ST T ransmitted-light Base Light source Halogen lamp 12V/20W Quick illuminant change Ye s Illuminated area 50 mm Po wer supply unit Input voltage 100–240 V~, frequenc y 50–60 Hz energy consumption 30 W max., ambient temperatur e 10 – 40 °C Connections Power plug , power switch W eight 7.4 kg Illuminati[...]

Leica M-series User Manual 146 Leica TL BFDF T ransmitted-light Base Light source External via cold light source Illuminated area 40 mm Connections Connection for cold light guide , active f=10mm, end tube f=13mm W eight 5.8 kg Illumination types Bright field Ye s Dark field Ye s Oblique light No Relief Contrast S ystem (RC™) No CCIC ( Constant[...]

Leica M-series User Manual 147 Leica TL RC™ / TL RCI™ Leica TL RC™ Leica TL RCI™ Light source External via cold light source Halogen lamp 12V/20W Quick illuminant change – Ye s Illuminated area 35 mm 35 mm Po wer supply unit – 100–240 V~, frequency 50–60 Hz energy consumption 30 W max., ambient temperatur e 10 – 40 °C Connections[...]

Leica M-series User Manual 148 Leica IsoPro™ Mot orized XY Stage Compatibility Leica inciden t light base and TL bases ( TL BFDF , TL RC™, TL RCI™) Stage size (L × W × H) 335.5 mm × 370 mm × 41.5 mm Tr a v e l 152 mm × 102 mm (6" × 4") Speed X,Y 30 mm/s Motor resolution X,Y 0.25 µm Repeatability X, Y ± 2 µm Positioning ac c[...]

Leica M-series User Manual 149 Appendix[...]

Leica M-series User Manual 150 Calculating the T otal Magnific ation and F ield of View Diameter Paramet er MO Magnification of the objective ME Magnification of the eyepiece z Magnification of the changer position q T ube factor , e .g. 1.5× for coaxial incident light, 1.6× for 45° Ergo T ube™ r F ac tor 1.25x if the planachromatic and pl[...]

Leica M-series User Manual 151 Care , Maintenance, C ontac t P ersons W e hope you enjoy using your high-perfor - mance microscope. Leica microscopes are renowned for their r obustness and long ser vice life. Observing the following care and clean - ing tips will ensure that even after years and decades, your Leica microsc ope will continue to work[...]

Leica M-series User Manual 152 Care , Maintenance, C ontac t P ersons (cont' d.) Protection from dirt Dust and dir t will affect the qualit y of your results. Put a dust cover over the microscope when ρ it will not be used for a long time. Use dust caps to protect tube openings, ρ tubes without eyepieces , and eyepieces. Keep accessories in[...]