Roper E manuale d’uso

57-061-001 Re v G0[...]

User Manual for CoolSNAP cf and CoolSNAP ES Systems[...]

57-061-001 Re v G0 © Copyright 2005 Roper S cientific , Inc. 3440 East Britannia Dr ive Tucson, Arizona 85706 Tel: 800.874.9789/520. 889.9933 Fax: 520.295.0299 All rights reserved. No part of this publication may be reproduced by any mea ns without the written permission of Roper Scientific, Inc. Printed in the United Sta tes of America . Co o l S[...]

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i LIMITED WARRANTY — Pho tometrics Analytical Instrumentation Photometrics , a division o f Roper Scientific, Inc. (“ Photom etrics,” us,” “we,” “our”) m akes the fo llowing limited warranties. Th ese limited warranties extend to the original purchaser (“Yo u”, “you”, "Your", "your") only and n o other pu[...]

ii Software Limited Warrant y Photometrics w arrants a ll of our man ufactured software di scs to be free f rom substantial defects in mate rials and / or workmanship und er normal use for a per iod of one ( 1) year fr om shipment. Photomet rics does not warrant that the function of the software will meet your requirements or that oper ation will b[...]

iii 8. After the warranty per iod has expir ed, you may contac t the Photometric s factory or a Photome trics- authoriz ed representative for repa ir informa tion and/ or extended warra nty plans . 9. Physically damaged units or units that have been modified are not a cceptable for repair in or out of warranty and will be retu rned as received. 10.[...]

iv Optional CoolSNAP Camera System Service Contract The optiona l Cool SNAP Camera Syst em Service Contra ct provides extended co verage for the Co olSNAP camera system. All te rms and condit ions listed in the CoolSNA P Camera System Warranty, including those pertaining to shipping costs and appropriate system use, apply to the CoolSNAP Ca mera Sy[...]

v Declaration of C onfo rmi ty Roper Scientific, Inc. decla res that th e equipment described in this documen t is a CLASS A DEVICE in conforman ce with the requ irements of the Euro pean Council Di rectives, listed below: 89/336/EEC EMC Dir ective & Ame ndments 93/6 8/EEC EMC Directive & Amen dments 73/23/EEC Product Safe ty with A mendmen[...]

vi Declaration of C onfo rmi ty Roper Scientific, Inc. decla res that th e equipment described in this documen t is a CLASS A DEVICE in conforman ce with the requ irements of the Euro pean Council Di rectives, listed below: 89/336/EEC E MC Directiv e 92/31/EEC Amend ment 93/68/EEC Amend ment 73/23/EEC Product Safet y on the application of th e laws[...]

vii Table of Contents Chapter 1. Introduction Description .................................................................................................................... ............. 1 System Components .............................................................................................................. ... 1 About This Manual ......[...]

viii Chapter 5. Basic Specifications Dimensions .............................................................................................................................. 15 CCD Specifications and O rientations ................................................................................... 17 Additional Measurements ........................[...]

1 Chapter 1 . Introduction Description CoolSNAP cf a nd CoolSN AP ES , fr om Photometrics ® , are ideal cameras for digital microscopy a nd many other biologica l applications. The sim ple, compact CoolSNAP cf incorporates a high-quality CCD (charge -coup led de vice ), a 12-b it scientific digitizer, and low-noise elec tronics to produce high-qua[...]

2 CoolSNAP cf /CoolSNAP ES About This Manual The CoolSNAP User Ma nual is divided in to five chapters . It is suggested that you read the entire ma nual before operatin g the camera in order to ens ure proper use. The chapters that fo llow this introduction are: • System Insta llation — Instructions for installin g the camera system’s hardwa [...]

Chapter 1. Introdu ction 3 Storage Require ments Store the CoolSNAP camera system in its original co ntainers. To protect the system fro m excessive hea t, cold, and moisture, sto re at an a mbient temperature betwee n 0°C and 70°C with a r elative hu midity of 0% -80% noncond ensing. Microscopes, Lenses , and Tripods The camer a has a standard t[...]

4 CoolSNAP cf /CoolSNAP ES Photometrics Customer S ervice If you have an y questions about your ca mera system, co ntact Photometrics Customer Service. When you call, please ha ve your Photometrics sales order number or equipm ent serial numbers availa ble. • Tel: 800. 874.9789/520. 889.9933 be twee n 8:00 am and 5:00 pm M ST • Fax: 520. 295.02[...]

5 Chapter 2. System Installation Carefully review the Precautions se ction on page 2 before performing a ny of the procedures outline d here. Again, use only a CoolSNAP cable and a CoolSN AP interface card w ith your CoolSNAP came ra . Using a different cable or interface card may result in permanen t damage to your sy stem. Introduction Yo u r Coo[...]

6 CoolSNAP cf /CoolSNAP ES If you are a Ma c user, the host computer for yo ur CoolSNAP camera must have the followin g: • Macintosh OS X.3 (Panther ) oper ating system • 512 MB RA M (or greater ) • CD-ROM drive • At least one un used PCI or PCI-X card slot (LVDS ca meras on ly) • At least one unus ed IEEE- 1394 port (I EEE- 1394 cameras [...]

Chapte r 2. Syst em In stallat ion 7 Connecting Your CoolSNAP Camera The CoolSNAP ca ble connects your Co olSNAP camera to the interface card. This cable is designed to serve as a co nduit both for da ta and pow er. CoolSNAP IEEE- 1394 Cable CoolSNAP LVDS Cable To conne ct your LVDS Coo lSNAP camera: 1. Conn ect either end of the Coo lSNAP LVDS cab[...]

8 CoolSNAP cf /CoolSNAP ES To connect your IEEE- 1394 CoolSNAP camera: 1. Connect either end of the Cool SNAP IEEE- 1394 cable to th e CoolSNAP IEEE- 1394 interfac e card that you have insta lled in the host computer. 2. Connect the other end of the CoolSN AP IEEE- 1394 interfac e cable to either DATA connector loca ted on the back of the camera (s[...]

9 Chapter 3. Operating Features Common Featu res The features described in this se ction are identical in the Co ol SNA P cf and CoolSNAP ES cameras . Features tha t are specific to the CoolSNAP ES camera a re described separa te ly in the "CoolSNAP ES " section. Readout Speed T h e CoolSNAP cf and CoolSN AP ES cameras operate at 20 MHz d[...]

10 CoolSNAP cf /CoolSNAP ES If the exposure time is 5 0 ms, which is less tha n the readout time, the camera operates in "nonoverlapped" mode (see Figure 2). The effective frame rate for this is 7.14 fps ( 1/[0.05+0.090] ). While in normal mode, the camer a firmware automatically calculates the readout time s, taking into acco unt binning[...]

Chapte r 3. Op eratin g Fea ture s 11 Application Ex amples Exampl e 1 "I have a C oolSNA P ES and wan t to operate the camera in the most sensitive setting for taking high-resol ution, single images." For this application, the ca m era should be o perated in "alternate no rmal" mode to provide the best q uantum efficiency and t[...]

12 Chapter 4. Troubleshooting If you have any difficulty while troubleshoot ing, or do not see yo ur camera syst em’s symptom s listed here, cont act P hotomet rics Custom er Service. System Does N ot Boot Normally If your operating system does not b oot normally after yo u have installed a interface card, try in stalling the new card in another [...]

13 Images Not Displayed Proper ly If the amber LED on the back of the came ra does not illuminate while images are being acquir ed and no image s appear: • Confirm that the green LED on the ba ck of the camera is illuminated, indicating th at the cam era is powered on . See Green LE D Does N ot Illuminat e (Camera H as No Power) on page 12. • C[...]

14 CoolSNAP cf /CoolSNAP ES IEEE- 1394 CoolSNAP Camera will Not Image When Attached to Certain Du al- processor or Hyperthr eading- capable PCs Some high-e nd Windows 2000/XP-e nabled comp uters with Int el Pentium or Xeon ™ processors include a memory enha ncement called Physical Addres s Extension (PAE). PAE allo ws these compters to a ddress m[...]

15 Chapter 5. Basic Specifications Dimensions C L Tripod Mount Hole on center with CCD (.25 in. - 20 UNC-2B) 4.50 in. (1 1.43 cm) 2.52 in. (6.40 cm) CoolSNAP Came ra: Front Vie w 5.01 in. (12.72 cm) 5.57 in. (14.15 cm) 2.10 in. (5.33 cm) Tripod Mount Hole (centered on face of camera) CoolSNAP LV DS Camera: Side V iew[...]

16 CoolSNAP cf /CoolSNAP ES CoolSNAP IEEE- 1394 Camera: Si de View[...]

17 CCD Specifications and Orie ntations CoolSN AP cf CoolSN AP ES Image Type Color and Mo no Mono Array Sony ICX 205AK (color ) Sony ICX205A L (mon o) Sony IC X285AL Resolution 1392 x 1040 1392 x 1040 Pixel Si ze 4.65 µm x 4.65 µm 6.45 µm x 6.45 µm Digiti zation Rate 20 MHz 20 MHz Readout Noise 15 e- rms @ 20 MHz 8 e- rms at 20 MHz Cooling Th e[...]

18 CoolSNAP cf /CoolSNAP ES Detector Rear Panel Description The following connectors, d isplay lights, an d controls are located on the back of the cameras (pictured below). DATA connector: for data transfer a nd powe r • LVDS cameras: 2 0-pin, high-dens ity connector • IEEE- 1394 cameras: 6-pin IEEE- 1394 connector EXPOSE OUT connector: BNC co[...]

Chapter 5. Basic Spec ifications 19 Auxiliary Power Supply Usage Re quirements The auxiliary CoolS NAP power supply (sho wn below) provides power to your CoolSNAP cam era, but is required only under certain conditions. If your CoolSNAP camera is connected to an I EEE- 1394 port that does not supply power (such a s a 4-pin connector on a laptop comp[...]

20 Index A Altern ate nor mal mod e · 10 Antiblooming · 1 0 Applic ation e xamples · 11 Auxiliary power su pply · 19 C Camera cable installa tion · 7 Camera description · 1 Camera features · 9 CCP orie ntation · 17 Cleaning exterior ca mera surfa ces · 3 imaging window · 3 Clocking modes altern ate no rmal · 10 nor mal · 9 Connecting yo[...]

57-061-001 Rev G 0 BENELUX Roper Scientific, BV Ir. D.S. Tuijnmanweg 10 4131 PN VIANEN, Netherlands tel: 31.347.324989 fax: 31.347. 324979 email: mailto@roperscientific .com FRANCE Roper Scientific, SARL Z.I. Petite Montagne Sud 4, rue de l'Oisans - C.E. 1702 91017 Evry Cedex, France tel: 33.160.86. 03.65 fax: 33.160. 86.07.09 email: princeton[...]