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GE UV-1 manual

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Table of contents for the manual

  • Page 1

    P H A R M A C I A B I O T E C H Monitor UV -1 User Man ual Edition A G 59-7797-01[...]

  • Page 2

    Important user information Reading this entire manual is recommended for full understanding and use of this product. ● The exclamation mark within an equilateral triangle is intended to alert the user to the presence of important operating and maintenance instructions in the literature accompanying the instrument. Should You have any comments on [...]

  • Page 3

    Contents 1. Int r oductio n ....................................................................................... . 4 2. General Descriptio n ........................................................................ . . 5 2. 1 Basic principl e ............................................................................ . 5 2. 2 Optical uni t .....[...]

  • Page 4

    1. Introduction 1. Introduction The UV-1 is a fixed wavelength UV monitor , consisting of a control unit and an optical unit that can be positioned up to 10 meters apart. A mercury lamp is the stable light source , furthermore a built-in reference cell eliminates baseline drift. The output signal can be recor- ded in either AU or %T. Sensitivity ra[...]

  • Page 5

    2. General Description 2. General Description 2.1 Basic principle GE Healthcare LKB Monitor UV-1 consists of an optical unit containing the flow cell , lamp , filter assemblies and preamplifiers , and a control unit containing the signal processing circuits. The two units are connected via a multi-core cable hardwired from the control unit to the o[...]

  • Page 6

    2. General Description Fig. 2 Block diagram The photocurrent from each detector is amplified in a pre-amplifier before passing to the signal processing circuitry in the control unit. If transmission is to be monitored , the signal passes directly to the low pass filter. If AU is to be monitored , the reference cell light intensity (IR) is compared [...]

  • Page 7

    2. General Description 2.2 Optical unit F r ont panel cont r ols Fig. 3. Optical unit. F ront panel. No. Item Description 1 Cel l holder The complete cell holder is rem o v ed b y tu r ning the lo c king (Fig . 4:6) knob on the rear panel 2 Sampl e inlet Inlet f or sample fl o w 3 Sampl e outlet Outlet f or sample fl o w 4 Re f erenc e inlet Inlet [...]

  • Page 8

    2. General Description Rear panel contr ols Fig. 4. Optical unit. Rear panel. No. Item Description 6 Locking knob The cell holder is in loc ked position when the loc king knob is turned fully in the direction of the arrow 7 Filter inlet Filters, con verters or aper tures are inser ted in the positions indicated. When inser ting them, align 8 Conv e[...]

  • Page 9

    2. General Description 2.3 Cont r ol unit F r ont panel cont r ols Fig. 5. Control unit. F ront panel. No. Item Description 12 Absorbanc e r ange Selector f or the desired absorbance r ange selector 0.01, 0.02, 0.05, 0.1, 0.2, 0.5, 1 or 2 A U full scale deflection . In the position SHO R T , the signal output te r minals are disconnected from the r[...]

  • Page 10

    2. General Description Rear panel controls Fig. 6. Control unit. Rear panel. No . Item Description 17 18 19 20 21 22 Mains voltage selector Fuse holder Signal outputs Mains inlet Optical unit inlet Ground terminal Selects mains voltage 110, 130, 220 and 240 V Mains fuses: 1 x 250 mA for 110-130 V 60 Hz 1 x 125 mA for 220-240 V 50 Hz The output sign[...]

  • Page 11

    3. Installation 3. Installation 3.1 Site UV-1 should be installed on a stable , flat surface away from all sour- requirements ces of vibration. The atmosphere should be free of both excess humi- dity and corrosive or contaminated vapours which may form deposits on the component in the optical path. UV-1 can be installed either in a coldroom or at a[...]

  • Page 12

    3. Installation Mains voltage Voltage selector setting Fuse 3.4 Installation of the filter 110 V 110 250 mA 130 V 130 250 mA 220-230 V 220 125 mA 240 V 240 125 mA 4. Select the mains cable corresponding to your mains outlet. Discard unwanted mains cable immediately. Connect the instrument to a grounded mains outlet. Note: Do NOT switch on. Note: Sp[...]

  • Page 13

    3. Installation 3.5 Installation of the flow cell Monitor U V -1 accepts flow cells for Standa r d Ch r omatograph y , FPLC and industrial applications. P r oduct Material of P ath T otal dead Illuminate d Pressure Application Code No. wetted pa r ts length v olume v olume limit area S-2 Fluoro-plasti c , 2 mm 80 µl 2 µl 0.3 M P a Standard 19-484[...]

  • Page 14

    3. Installation 3.6 3.7 Connecting The optical unit may be placed on the bench or mounted on the optical unit laboratory scaffolding. For scaffolding mounting , mount the support rod on the optical unit. The rod may be mounted horizontally or vertically (Fig. 4:9). Tighten the the Allen screw firmly. The optical unit should be placed as close as po[...]

  • Page 15

    4. Operation 4.1 Choice of wavelength 4. Operation The UV-1 can be operated at either 2 5 4 nm , 280 nm or 40 5 nm. The choice of wavelength will depend on the spectral properties of both the eluent and the substances to be detected. Proteins and poly- pep-tides containing aromatic amino acids are usually best detected at 280 and 2 5 4 nm. Nucleic [...]

  • Page 16

    4. Operation 4.3 Conversion T% AU OD OD table T% to AU (3 mm cell) (10 mm cell) and OD 1 5 10 15 20 25 30 32 35 40 45 50 55 60 63 65 70 75 79 80 85 89 90 95 95.5 97.7 2.000 1.301 1.000 0.824 0.699 0.602 0.523 0.500 0.456 0.398 0.347 0.301 0.260 0.222 0.200 0.186 0.155 0.125 0.100 0.097 0.070 0.050 0.046 0.022 0.020 0.010 6.667 4.337 3.333 2.747 2.3[...]

  • Page 17

    4. Operation 4.6 Basic operating procedure - AU 4.7 Basic operating procedure - Transmission (%T) 4.8 Shut down 1. 2. 3. 4. 1. 2. 3. 4. 5 . 6. 7. 1. Switch AU/ %T to AU (Fig. 5 :13). Set the range selector (Fig. 5 :12) to SHORT and zero the recorder with the recorder zero control. Set the range selector to 2 and adjust the recorder baseline with th[...]

  • Page 18

    5. Maintenance 5. Maintenance 5.1 General To ensure trouble free running , users are advised to observe the follo- precautions wing precautions: All liquids passing through the flow cell should be free of suspended particles. All liquids should be degassed to prevent air bubble formation in the flow cell. Never allow buffer solutions to dry out in [...]

  • Page 19

    5. Maintenance Cleaning with detergent: ● 1. Remove the cell holder from the optical unit. 2. Pump undiluted cleaning detergent through the cell for at least 2 hours. 3. Rinse the cell with a) distilled water (100 ml) b) ethanol/distilled water ( 5 0% v/v , 100 ml) c) distilled water (100 ml) 4. Replace the cell in the optical unit and reconnect [...]

  • Page 20

    8. Insert the new cell, being careful not to touch either of the optical surfaces. See that the inlet and outlet ports are corr ectly aligned. 9. Retighten the screw washers with the tool pr ovided. Do not use excessive force. 10. Check for leakage. 1 1. Replace the black cover . For optimum performance, it is essential that the interference filter[...]

  • Page 21

    4. Set the range selector to SHORT and zer o the recor der . 5 . Set the range selector as follows: 2 5 4 nm , range 1 280 nm , range 0.2 6. T urn Zer o fully clockwise. Response 8 mV or greater: the lamp is operating properly and the optical system is clean. Response 0 mV or very close to 0 m V : Contact a GE Healthcare r epresentative. Response l[...]

  • Page 22

    5. Maintenance 5.9 6 . Use an Allen key (2. 5 mm) to unscrew the upper of the two screws securing the lamp holder and the lamp. Note carefully the positions of the insulation sleeve and washer. 7. Disconnect the lamp from the PC-board. 8. Bend the upper part of the lamp holder slightly (2-3 mm) upwards and withdraw the mercury lamp from it by gentl[...]

  • Page 23

    6. Trouble-shooting 6. Trouble-shooting The UV-1 Monitor has been designed for trouble-free use. If good chromatographic practice is followed , very little difficulty should be experienced. Clean optical surfaces are essential if low noise levels are to be maintained. The following check list of the most frequent problems is meant to be a guide in [...]

  • Page 24

    7. Technical Specifications 7. Technical Specifications Wavelength range Lamp Filters Operating modes Full scale ranges Noise Linearity Temperature drift Long term drift Time constant Recorder output Environment Power consumption Power supply , voltage frequency Dimensions (LxWxD) Weight 2 5 4 nm , 280 nm and 40 5 nm Hg lamp: for 2 5 4 , 280 , and [...]

  • Page 25

    8. Accessories and Spare Parts 8. Accessories and Spare Parts Please order accessories and spare parts according to the designation and code numbers given below. Designation Filters Filter kit, 254 nm Filter kit, 280 nm Filter kit, 405 nm Aperture Converter 280 nm Flow cells Flow cell S-2 complete with measuring cell Flow cell 10 mm complete with m[...]

  • Page 26

    Printed in Sweden by SNITS & DESIGN AB/Västra Aros Tryckeri, Nov 1996[...]