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Table of contents for the manual
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Page 1
Lorem Ipsum Leica ES2 Leica EZ4 Leica EZ4 HD Manual[...]
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Leica E Series Manual 2 Congr atula tions! Y ou have just purchased a L eica E Series stereo- microscope - an excellen t choice! The Leica E series is per fectly suited for practical instruc- tion in schools and universities as w ell as f or routine tasks in the laboratory and industrial production and testing. Viewing entire objec ts under magni?[...]
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Leica E Series Manual 3 Chapter Overview Safety and Use 6 Leica ES2 10 Leica EZ4 and Leica EZ4 HD 18 Eyepiec es (only for Leica EZ4) 29 Photograph y Using the Leica EZ4 HD 37 The Camer a Menu 51 Care and T ransport 62 Specications 64 Dimensions 67[...]
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Leica E Series Manual 4 Leica ES2 Overview : Leica ES2 11 T ranspor t, Placement and S torage 12 The Corr ect Interpupillar y Distance 13 Using the LED illuminator 14 F ocusing 15 Magnication Display 16 Adjusting the Resistance of the F ocus Drive 17 Leica EZ4 and Leica EZ4 HD Overview : Leica EZ4 19 Instrument variant: Leica EZ4 HD 20 T ranspor[...]
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Leica E Series Manual 5 Con tents ( Continued) The Camer a Menu Optional Remote Contr ol 52 Viewing Images and Movies with the Optional Remote C ontrol 53 Calling up the Camera Menu 54 COL OR (Automatic White Balance) 55 COL OR (Manual Whit e Balance) 56 EXPOSURE 57 RESOL UTION 58 SETUP CAMERA (Camera Settings) 59 SETUP USER (User-dened Settings[...]
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Leica E Series Manual 6 S af ety and U se[...]
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Leica E Series Manual 7 Before installing, operating or using the instrument, it is mandatory to read this user manual. In particular , please observe all safety instructions. The "S af ety Concept" brochure contains additi- onal safety information r egarding service work, requirements and the handling of the micro- scope, accessories[...]
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Leica E Series Manual 8 Intended use The E Series microscopes are optical instru- ments for improving the visibility of objec ts through magnication and illumination. It is used for observing and documenting. Place of use Use the instrument in enclosed, dust-free rooms at +10 °C to +40 °C only . Pr otect it from oil, chemicals and extreme h[...]
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Leica E Series Manual 9 Intervention from the user The microscopes of the E Series contain no components that can be maintained or repaired by the user. Exc eptions are menti- oned specically in this manual. Unless other wise specied in this manual, repair and ser vice work may be carried out by authorized Leica technicians only . [...]
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Leica E Series Manual 10 L eica ES2[...]
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Leica E Series Manual 11 Overview: Leica ES2 1 2 3 5 7 6 4 1 10× xed eyepiec es for eyeglass wear ers 2 Carrying handle 3 T wo-level magnication changer 10× and 30× 4 Focusing driv e 5 Integrat ed LED incident light illumination 6 Integrat ed LED transmitted illumination 7 Switch for incident and tr ansmitted light[...]
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Leica E Series Manual 12 Built-in carrying device All Leica E Series microscopes are equip - ped with a carr ying handle for conveni- ent and safe transport. Setting up the microscope 1. Place the microscope on a a t tabletop . 2. Connect the power supply to a grounded socket. T ransport, Placement and Stor age Packing the device a way 1. Af[...]
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Leica E Series Manual 13 Setting the eye distance 1. Look into the ey epieces. 2. Hold the eyepieces with both hands. Push the eyepieces t ogether or separate them until you see a circular image . Note for ey eglass wear ers If you wear glasses , f old the ey ecups back – otherwise, fold the eyecups forwards. The C orrect Interpupillary D ist[...]
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Leica E Series Manual 14 Iluminating the specimen 1. Position a specimen in the middle of the baseplate. 2. Switch the two LED illumina tors on or o as desired. After switching o , the LEDs remain lit f or about another 4 seconds. Tips on use Ϙ Use incident light for plastic, opaque objects. Ϙ Use transmitted light for transmitted light[...]
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Leica E Series Manual 15 F ocusing raises and lowers the stereomi- croscope using the focusing drive. The specimen is brought into sharp f ocus as soon as it is in the focal point of the objective. The focusing drive can be operated either lef t- or right-handed. Focusing 1. Set the magnication changer to the lowest level (10). 2. Set the wo[...]
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Leica E Series Manual 16 Magnication Display Y ou can read the congured magnica- tion on the right -hand rotary knob of the Leica ES2. The additional magnication provi- ded by the eyepieces is already incorporated in the scale.[...]
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Page 17
Leica E Series Manual 17 Adjusting the Resistanc e of the F ocus Drive Adjusting the resistance Is the focusing drive too easy or too dicult to adjust? No problem - the resistanc e can be adjusted individually depending on your perso- nal prefer ences: 1. Grip the drive knobs with both hands and turn them towar ds each other until the desired re[...]
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Leica E Series Manual 18 L eica EZ4 and Leica EZ4 HD[...]
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Leica E Series Manual 19 Overview: Leica EZ4 1 2 3 5 7 6 4 1 10x xed eyepiec es for eyeglass wear ers 2 Carrying handle 3 Magnication changer 4 Focusing driv e 5 Integrat ed LED incident light illumination 6 Illumination control 7 Integrat ed LED transmitted illumination[...]
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Leica E Series Manual 20 Instrument varian t: Leica EZ4 HD 1 2 3 5 6 9 8 7 4 1 10x xed eyepiec es for eyeglass wear ers 2 Integrat ed digital camer a with SD card slot 3 Carrying handle 4 Magnication changer 5 IR receiv er for optional remote c ontrol 6 Focusing driv e 7 Integrat ed LED incident light illumination 8 Illumination control 9 Int[...]
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Page 21
Leica E Series Manual 21 Built-in carrying device All Leica E Series microscopes are equip - ped with a holder for convenien t and safe transport. Setting up the microscope 1. Place the microscope on a a t tabletop . 2. Connect the power supply to a grounded socket. T ransport, Placement and Stor age Packing the device a way 1. After use, wi[...]
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Page 22
Leica E Series Manual 22 The C orrect Interpupillary D istanc e Setting the eye distance 1. Look into the ey epieces. 2. Hold the eyepieces with both hands. Push the eyepieces t ogether or separate them until you see a circular image . Note for ey eglass wear ers If you wear glasses , f old the ey ecups back – otherwise, fold the eyecups forw[...]
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Leica E Series Manual 23 Automatic shut o If the illumination is not operated for 60 minutes, it switches o automatically . The illumination can be r eactivated by pr essing a button on the membrane keypad. LED illumination 1. Position a specimen in the middle of the baseplate. 2. Switch the two LED illumina tors on or o as desired: Ϙ[...]
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Leica E Series Manual 24 Disabling the automatic shuto T o disable the automatic shuto , follow these steps: 1. Switch the microsc ope o . 2. Press and hold the and buttons on the light control unit simultaneously while you switch on the micr oscope. The LEDs ash twice slowly and then remain switched on. The automatic shut o is now d[...]
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Leica E Series Manual 25 Three-level incident light illumina tion The incident light illumination is made up of 5 LEDs. T wo, three or v e diodes are switched on depending on the level. A ccord- ingly , the angle of incidence of the light beam on the object is switched from high to low . Illumination types Press 1× Five diodes: gr eatest br[...]
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Leica E Series Manual 26 F ocusing F ocusing raises and lowers the stereomi- croscope using the focusing drive. The specimen is brought into sharp f ocus as soon as it is in the focal point of the objective. The focusing drive can be operated either lef t- or right-handed. Focusing 1. Set the magnication changer to the lowest level (8). 2. S[...]
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Leica E Series Manual 27 Magnication Display Y ou can read the congured magnica- tion on the right -hand rotary knob of the Leica EZ4 models. The additional magnication provided by the eyepieces is already incorpor a- ted in the scale Leica EZ4 models with open tubes On the EZ4 model without eyepieces, only the magnication fa[...]
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Leica E Series Manual 28 Adjusting the Resistanc e of the F ocus Drive Adjusting the resistance Is the focusing drive too easy or too dicult to adjust? No problem - the resistanc e can be adjusted individually depending on your perso- nal prefer ences: 1. Grip the drive knobs with both hands and turn them towar ds each other until the desired re[...]
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Leica E Series Manual 29 E y epiec es (only f or L eica EZ4)[...]
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Leica E Series Manual 30 Changing eyepiec es V arious eyepieces are available for the Leica EZ4, allo wing the st ereomicroscope to be adap- ted even better t o the specic requirements . The following eyepiec es ar e available for selec- tion: Ϙ Eyepieces replac eable, xed or adjustable: 10×/20, 16×/16 or 20×/12 Ϙ Eyepieces for eyeglass [...]
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Leica E Series Manual 31 Ey epieces and E y ecups ( Only for Leica EZ4, Open V ersion) Eyeglass eyepiec e with adjustable diopter and straight eyecups Eyepiec e for users who do not wear eyeglasses with adjustable diopter and inclined eyecup Fix ed eyepiece with inclined eyecup . Eyecups The eyecups can be r eplaced. Risk of infection Direct co[...]
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Leica E Series Manual 32 Dioptric Correction The adjustable eyepieces for the Leica EZ4 are also available with built-in diop - tric corr ection, allowing the microscope to be used without glasses even by those with vision problems. The correction comprises a maxi- mum of ±5 diopter settings. Users with normal vision do not need adjus- table e[...]
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Leica E Series Manual 33 Dioptric Correction ( Con tinued) 7. Look into the ey epiece. 8. Slowly rotate the eyepiece individually in the "–" direction until the eye sees the object sharply imaged. 9. Select the highest magnication and ref o- cus if necessary. Now , if you adjust the magnication from the highest to the lowest level[...]
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Leica E Series Manual 34 Graticules ( O nly f or Leica EZ4, Open V ersion) Graticul es The transparent graticules can be inser- ted in the adjustable eyepieces of the Leica EZ4. They enable easy measurements at the specimen. F or equipment with a graticule in one eyepiece , two adjustable eyepieces are needed. Since one ey e is focussed on [...]
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Leica E Series Manual 35 V ariant 2: 16× ey epieces On the 16× adjustable eyepieces, the inser t is xed to the ey epiece by a screw ed connection. 1. Screw the insert out counterclockwise. V ariant 1: 10× and 20× ey epieces The 10× and 20× adjustable eyepieces include an insert at the bottom with a snap mount. 1. Gently pr ess the two smal[...]
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Leica E Series Manual 36 Inser ting the graticules is the same for all eyepi- ece types. 1. Place the insert with the knurled side on the work surface. 2. Grasp the graticule so that you see the scale in laterally corr ect form. 3. Carefully take hold of the edge of the grati- cule and push it sideways into the holder in the insert. Make sure tha t[...]
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Leica E Series Manual 37 Photogr aph y U sing the Leica EZ4 HD[...]
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Leica E Series Manual 38 Leica EZ4 HD: Overview of the instrument 1 3 2 4 1 SD card slot 2 Status LED 3 Black multifunction button 4 Red multifunction button 1 3 2 1 USB connection 2 Pinhole switch 3 HDMI connection[...]
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Page 39
Leica E Series Manual 39 General Instructions General Instructions Ϙ The software for control is included on the DVD provided with the instrument . Ϙ Install the software before connecting the Leica EZ4 HD to the PC or Mac. Ϙ As soon as the EZ4 HD is connec ted to a power cable and the main switch is turned on, the integr ated camera is also rea[...]
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Leica E Series Manual 40 The Leica EZ4 HD puts some extremely interes- ting additional func tions at your ngertips. The integrat ed digital camera is designed for the sole purpose of giving you the best possible quality for your documentation – with par ticu- larly easy handling. Real Full HD displa y The integrated HDMI output allows the micr[...]
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Leica E Series Manual 41 Please do not discard any of the packing materials. They should be used for safely storing and transporting the instrument should the need arise. DVD with Leic a software , drivers and manuals USB cable HDMI cable SD card Optional: Remote control with battery ( Type CR2025) Standard deliv ery[...]
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Page 42
Leica E Series Manual 42 The camera is normally in what is known as "HD mode" after being switched on. In this mode, it outputs a high-resolution signal in 16:9 wide- screen format. The images can then be saved directly to an SD card. If the Leica EZ4 HD is connected to a computer via USB and the Leica software provided is star- ted, the [...]
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Leica E Series Manual 43 Saving to SD card Images can be saved directly to an inser- ted SD card. The card has to be formatt ed using the "F A T" le system. When the SD memory card is full, the LED on the camera is illuminated in red. No more images can be captured . Changing between PC and HD mode In order to take phot os [...]
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Leica E Series Manual 44 Capturing an image 1. Inser t the SD memory card into the SD car d slot until you hear it click into position. The LED illuminates in green. The camera is now ready to sav e images to the SD card . 2. Press the red image capture button briey . Y ou hear an audible signal as conrmation and the camera LED ashes green[...]
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Leica E Series Manual 45 Cables 1. Connect the camera and the HDMI device via the integrat ed HDMI port. The HDMI cable required for this purpose is included in the standard deliv ery of the Leica EZ4 HD . Live images and captured images can be output in high resolution via the HDMI cable: Ϙ F ull HD: 1920×1080 pixels Ϙ HD ready: 1280×7[...]
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Leica E Series Manual 46 F or instructions on operating the instru- ment in PC mode, refer to the DVD inclu- ded in the standard deliver y of the Leica EZ4 HD. The 10× eyepieces of the Leica EZ4 HD have a eld of view of 20 mm. Unlike the circular object eld through the eyepieces, the image section of the camera is rectangular and has[...]
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Leica E Series Manual 47 Aspect ratio If the Leica EZ4 HD is connected to a computer and is in PC mode, images are always output in a 4:3 size ratio and in a low er resolution – even if a 16:9 monitor or television set is connected via HDMI. The image is neces- sarily compressed on such a device . In this case, we recommend that you not conne[...]
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Leica E Series Manual 48 In PC mode, the camera image is alwa ys shown in the preview window of the Leica soft war e. In addition, another computer monitor or television can be connected via the HDMI interface. The image resolution on the attached HDMI or television scr een is limited t o the live image resolution inside the LAS EZ software[...]
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Leica E Series Manual 49 W e rec ommend carrying out a new white balance adjustment whene - ver you change the illumination type or color temperatur e. This is par ticularly necessary if you are working with an ex ternal light source that can be switched from yellow (low inten- sity) to blue (high intensity). T he new white balance generates a neut[...]
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Leica E Series Manual 50 Restoring F ac tory Settings F actory settings 1. Press the red multifunction button for 10 seconds to delete all individual settings and reset the instrument to the factor y settings. 10 sec.[...]
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Leica E Series Manual 51 T he C amer a Menu If you purchased the optional remot e, y ou will have ac cess to the camera menu.[...]
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Leica E Series Manual 52 The remote control does not work if the camera is in PC mode. 1. Start video recording 2. Stop video rec ording 3. Save still image to SD car d 4. Stop/continue liv e image Pause/play mo vie clip 5. Playback mode 6. Show / hide information box 7. Arrow keys f or navigation 8. OK / conrm 9. Call up the camer a menu 1 [...]
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Leica E Series Manual 53 3. T o return to live view pr ess any button on the camera or press the key 1, 2 or 9 on the remote con trol (see previous page). If y ou have pur chased the optional remote control , you can display the images and videos captured by the HD camera directly on a HD screen. 1. Miniature view: (press 1×) Ϙ Select miniatu[...]
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Leica E Series Manual 54 The remote control does not work if the camera is connected to a computer and is in PC mode. The menus of the camera ar e also no longer displayed on the monitor . In this case, press the black multifunction button for 5 seconds to switch to HD mode (refer also to page 42). Opening and closing the camera menu 1. Poin t [...]
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MAIN MENU SUBMENU Leica E Series Manual 55 The "COL OR" function makes it possible to adapt the camera to the ambient light so that color-neutr al images can be acquired. Enabling automatic white balanc e 1. Press the ▤ butt on on the remote con trol. 2. Call up the "C OL OR" entry . 3. Set the value for "WB MODE"[...]
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MAIN MENU SUBMENU Leica E Series Manual 56 The "COL OR" function makes it possible to adapt the camera chip to the ambient light so that color -neutral images can be acquired . Adjusting the white balance manually (r ecommended) 1. Move the specimen out of the eld of view so that only the illumina- tion is seen. 2. Press the ▤ bu[...]
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Leica E Series Manual 57 MAIN MENU Main menu SUBMENU Submenu Manual exposure 1. Press the ▤ butt on on the remote con trol. 2. Call up the command for manual e xposure (see diagram at the left). 3. Set the value for "EXP MODE" to "M ANUAL " . 4. Correct the values for "EXPOSURE" , "GAIN" and "GAMMA &qu[...]
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Leica E Series Manual 58 MAIN MENU SUBMENU 2048X1536 1280X1080 720X480 HIGH HIGH The "RESOLUTION" menu enables you to dene the resolution for the live image on the computer , still image capture and video capture individually . T his provides optimum results in ev ery situation. LIVE (resolution of the live image) Denes the reso[...]
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Leica E Series Manual 59 Main menu Submenu The camera settings enable you to control internal parameters such as the date or time or to reset the camera. Y ou can also switch the camera from HD mode to PC mode ( or vice versa). SET DA TE TIME This command sets the date and time as well as the forma t for display- ing them. The following formats[...]
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Leica E Series Manual 60 Main menu Submenu The user-dened settings enable you to, for example, sa ve illumina- tion scenarios and retriev e them later . SET ILLUM -1 t o 3 (save illumina tion modes) These settings allow you to store up t o three di erent illumination modes and retriev e them later . Select the number of the illumination m[...]
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Leica E Series Manual 61 The camera in the Leica EZ4 HD provides various illumination modes that cover a wide variety of possible situations. Changing the illumination mode 1. Press the black multifunction button briey to display the current illumination mode on the monitor or T V set. 2. Press the button again to switch to the ne xt illumin[...]
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Leica E Series Manual 62 Car e and T r anspor t[...]
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Leica E Series Manual 63 General Instructions Ϙ Keeping all optical components clean is important for maintaining good optical per formanc e. Ϙ Always prot ect the microscope and camera with the plastic cover when the instru- ment is not in use. (Included in the delivery package of the microscope.) Be sure power to the camera is o when the dus[...]
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Leica E Series Manual 64 Specic a tions[...]
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Leica E Series Manual 65 Camera Module Camera W eight 700 g (camera only) Height 50mm Exposure time 2 msec – 2 sec Live image Max. 45 fps (1256 × 720 px) – 20 fps (1920 ×1080 px) Full frame image ac quisition 2048 × 1536 pixels, 3.1 megapixels Movie clip 720 × 480 pixels (MP4) Sensor size 6.55 mm × 4.92 mm Pixel siz e 3.2 μm × 3.2 μm Se[...]
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Leica E Series Manual 66 Mechanical and optical inter faces Ϙ Integrated camer a module Ϙ Light distribution Optical 50% / Camera 50% Ϙ Internal video magnication 0.5× Ϙ Sensor Center ed to optical axis ± 0.1 mm Ϙ Hoizontally / Vertically aligned ± 1 degree Electronic interfaces Ϙ USB 2.0, mini-USB plug Ϙ Mini-HDMI (HD ready and F ull [...]
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Leica E Series Manual 67 Dimensions[...]
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Leica E Series Manual 68 Leica ES2[...]
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Page 69
Leica E Series Manual 69 Leica ES2 ( C ontinued)[...]
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Leica E Series Manual 70 Leica EZ4 HD[...]
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Leica E Series Manual 71 Leica EZ4 HD ( C ontinued)[...]
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Leica E Series Manual 72 Leica EZ4, 10× Ey epieces[...]
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Leica E Series Manual 73 Leica EZ4, 10× Ey epieces (C ontinued)[...]
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Leica E Series Manual 74 Leica EZ4 without Ey epieces[...]
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Leica E Series Manual 75 Leica EZ4, without Ey epieces (C ontinued)[...]