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Leica L2 manual

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Table of contents for the manual

  • Page 1

    Leica StereoZoom ® User Manual[...]

  • Page 2

    Leica StereoZoom User Manual 2 Contents Page Information Safety rules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7 Description: Features, lens system . . . . . . . . . . . . . . . . . . .8 Models . . . . . . . . . . . . [...]

  • Page 3

    Leica StereoZoom User Manual 3 Dear Customer Thank you for the trust you have shown in choosing one of our products. W e hope you will have much enjoyment and success with your new stereomicroscope from Leica Microsystems. With its new StereoZoom ® line the Swiss Stereomicro- scopy Business Unit offers a comprehensive range of stereomicroscopes fo[...]

  • Page 4

    Read the instructions for use and safety instructions before putting the stereomicroscope into operation. Leica S4 E, S6 E, S6, S6 T , S6 D and S8 APO stereomicroscopes are precision optical instruments for making technical and scientific objects, object details or specimens more easily visible by means of magnification. Stands, illumi- nators and [...]

  • Page 5

    5 Leica StereoZoom User Manual W orkplaces with stereomicroscopes facilitate and improve the viewing task, but they also impose high demands on the eyes and holding muscles of the user . Dependent upon the duration of uninterrupted activity , this may lead to asthenic trouble and muscular -skeletal pain so that suitable measures must be taken to re[...]

  • Page 6

    Make sure that the instrument is set for the correct mains voltage. Incorrect setting can damage the instrument. Only devices with the correct power rating may be connected to the transformers. Overloading can result in damage to the instrument. • Disconnect the mains cable from the mains supply before changing lamps. • Never put your hand in t[...]

  • Page 7

    7 Leica StereoZoom User Manual User Manual Y our instrument is accompanied by a printed English user manual. Additional language versions and information can be found on the interactive CD-ROM. User manuals and updates are available for download on our homepage at www .stereozoom.com. Y ou will find the address of the closest local representative a[...]

  • Page 8

    The optical system of the Leica StereoZoom ® line con- sists of two beam paths converging at 12°. Since the pairs of objectives are very close together , the stereo- microscopes can be constructed very “slim” towards the base. This has a number of advantages: less space required for use on bonders and machines, unimpeded working on the object[...]

  • Page 9

    Features StereoZoom ® Zoom Magnification Viewing Extras angle Leica S4 E 4.8:1 6.3 × –30 × 38° ErgoLens Leica S6 E 6.3:1 6.3 × –40 × 38° ErgoLens Leica S6 6.3:1 6.3 × –40 × 60° ErgoLens Leica S6 T 6.3:1 6.3 × –40 × 38° T erminator ErgoLens Leica S6 D 6.3:1 6.3 × –40 × 38 ° Video/photo tubes Ergono- mic objectives Leica S8 [...]

  • Page 10

    10 Leica StereoZoom User Manual – Description StereoZoom ® Leica S4 E StereoZoom ® Leica S6 E StereoZoom ® Leica S6 Leica Design by Christophe Apothéloz[...]

  • Page 11

    11 Leica StereoZoom User Manual – Description StereoZoom ® Leica S6 T StereoZoom ® Leica S6 D StereoZoom ® Leica S8 APO[...]

  • Page 12

    12 Leica StereoZoom User Manual Design Leica StereoZoom ® System diagram 2 Leica S6 E 10 446 294 Leica S6 10 446 295 10 445 363 10 445 367 10 446 359 10 445 387 10 446 394 10 446 339 10 446 340 10 446 341 10 447 106 10 446 395 10 446 228 10 446 301 10 446 303 10 447 040 10 315 306 10 446 347 10 446 348 Low Eyepoint Eyepieces Eyepieces, fixed Eyepi[...]

  • Page 13

    13 Leica StereoZoom User Manual 3 Leica S6 T 10 446 296 Leica S6 D 10 446 297 10 446 342 10 446 343 10 446 344 10 446 345 13 312 610 10 446 299 10 376 070 13 312 714 High Eyepoint Eyepieces Eyepieces, fixed Eyepieces, adjustable* ergonomic ergonomic 10 × /23 10 447 136 10 × /23 10 447 137 * Adjustable eyepie c es a cc ept r eti c les 16 × /15 10[...]

  • Page 14

    1 Incident-light base with stage plate 2 T ransmitted-light base with glass stage plate 3 Focus column with microscope carrier 4 StereoZoom ® optics carrier 5 Optional additional objective 6 Eyepieces, fixed and/or adjustable The components 14 Leica StereoZoom User Manual Design, Basic equipment 5 4 3 2 1 6[...]

  • Page 15

    Never loosen the 3 screws on the right-hand side of the focus column. 왘 Remove stage plate. 왘 Insert 3 hexagon head screws through the under- side of the baseplate and screw tight in the focus column. 왘 Replace stage plate. 왘 Remove glass stage plate. 왘 Undo locking device. 왘 Place incident-light stand on the transmitted-light base and [...]

  • Page 16

    왘 Insert optics carrier carefully in the microscope carrier . 왘 Fix optics carrier in the desired position with the clamping screw . 왘 Screw selected objective tight in counterclockwise direction. 왘 Screw the lens shield tightly into the thread on the Stereo- Zoom ® or onto the additional objective. 왘 Push eyepiece into the eye- piece tu[...]

  • Page 17

    Soft eyecups Leica L2 cold-light source ➔ Stand High-performance lighting 17 The eyepieces are supplied with soft eyecups, which can be attached to achieve the following: – Prevent eye infections if several users are working at the same instrument (see p. 5). – Protect your glasses from being scratched. With its fibre-optic light guides the L[...]

  • Page 18

    18 The following graticules and stage micrometers are available for calibration: Graticule 10mm/0.1mm Graticule 5mm/0.1mm Graticule 5mm/0.05mm Graticule 100 div ./0.002" Graticule 100 div ./0.001" Graticule 150 div ./0.0005" Crosshair Stage micrometer 50mm, 0.1/0.01mm divisions Stage micrometer 1", 0.001" divisions For phot[...]

  • Page 19

    19 Leica StereoZoom User Manual – Assembly 왘 Pick up the graticules at the edge so as not to leave fingerprints and slide into the holder from the side. 왘 Put the insert back into the eyepiece and press firmly in place. 왘 Insert the eyepiece into the eyepiece tube and line up the graticule by turning the eyepiece in the eyepiece tube. The m[...]

  • Page 20

    20 Leica MPS30 / MPS60 Photoautomats 11 541 038 0,55 × – 1,1 × 11 543 703 11 543 706 10 445 932 10 446 120 8 × 10 445 304 10 × 10 445 305 16 × 10 404 207 10 445 541 10 162 226 Adapter SLR SLR camera housings 10 445 929 1/2” , 0,5 × 10 445 928 1/3” , 0,32 × 11 541 006 1”, 1 × 11 541 007 2/3”, 0,63 × 11 541 016 1/2” , 0,5 × 1”[...]

  • Page 21

    21 Leica StereoZoom ® S6 D and S8 APO are equipped with a built-in video/photo tube that allows for simple, quick set- ting up of digital, film and analog video cameras. Please ask your Leica advisor about the options. Y ou can find detailed information about Leica camera systems, accessories and image management software in the corresponding manu[...]

  • Page 22

    22 1 Magnification changer , right-hand pinion knob with magnification scale 2 S6 models: zoom limiter stop 3 Focusing drive 4 Fixing screw fixes the optics carrier in the microscope carrier 5 Adjustable eyepiece tubes: Interpupillary distance adjustable from 55–75mm 6 Eyepieces and eyecups 7 Lamp bracket mounting thread (on both sides and at rea[...]

  • Page 23

    23 • Pay great attention when setting up your stereomicro- scope. Y ou will only benefit fully from the outstanding optical and ergonomic advantages of the instrument if you set it up precisely in accordance with the directions already given. • Arrange your workplace as well as possible, and pay attention to the heights of chair and table. • [...]

  • Page 24

    24 The eyepieces are supplied with detachable eyecups (see also p. 5). If you do not wear spectacles and want the eyecups to fit snugly round your eyes: 왘 Attach the eyecups to the eyepieces. Y ou wear spectacles for your work. 왘 Fold the eyecups downwards. • The folded-down eyecups will protect your spectacle lenses against scratching. T o f[...]

  • Page 25

    25 Leica StereoZoom User Manual – Operating Is the focusing drive too stiff/too loose or does the set-up slip down unassisted? Y ou can tension the focusing drive to suit the weight of the set-up and your own needs: 왘 T ake one pinion knob in each hand and turn in opposing directions until the desired tension is reached. The optics carrier can [...]

  • Page 26

    Leica StereoZoom User Manual 26 Operating On the S6 models and for the S8 APO it is possible to fix the maximum and minimum zoom. The same function can be used to set a fixed level of magnification. 1. Set the minimum zoom level at 1 using the stop on the left-hand pinion knob: 왘 Loosen the hexagon head screws on the left-hand pinion knob using t[...]

  • Page 27

    27 Leica StereoZoom User Manual – Operating 2. Set the maximum zoom level at 3.2 using the stop on the right-hand pinion knob: 왘 Loosen the hexagon head screws on the right-hand pinion knob. 왘 T urn the right-hand pinion knob to 3.2. 왘 Move the stop on the right- hand pinion knob backwards until it touches the built-in zoom stop. 왘 T ight[...]

  • Page 28

    28 Operating Diopter adjustment and setting parfocality Preparations Bring the test object into focus If you adjust the diopters at the adjustable eyepiece as described below , the focus will remain constant, or par- focal, from minimum to maximum magnification. In other words, you will not have to refocus when you change magnification, but only wh[...]

  • Page 29

    29 Leica StereoZoom User Manual – Operating 왘 Without looking into the eye- pieces, turn the eyelens of the adjustable eyepiece as far as possible in the “+” direction. 왘 Close the eye that is looking into the fixed eyepiece and look into the adjustable eyepiece with the other eye. 왘 View the test object and slowly turn the eyelens cloc[...]

  • Page 30

    30 Leica StereoZoom User Manual Special notes Setting up diopter with two adjustable eyepieces 왘 For Leica S6 D and S8 APO, set pinion knob to Vis position. 왘 Set approximate working distance by means of focusing drive (working distances of various objectives see p.38–40). 왘 Set up lighting 왘 Set viewing distance (p.23). 왘 Set ‘0’ d[...]

  • Page 31

    Leica StereoZoom User Manual – Special notes 31 왘 Set lowest magnification. 왘 Do not look into the eyepiece! 왘 T urn lens counterclockwise in the ‘+’ direction until it stops. 왘 Now look into the eyepiece. 왘 Close one eye. 왘 With the other eye, observe the test object and turn the lens slowly clockwise in the ‘–’ direction, [...]

  • Page 32

    32 It is possible to switch between the observation and photo beam paths. Light distribution: – in ‘Vis’ position: 100% light in both eyepieces/no light in the video/photo beam path – in ‘Doc’ position: 100% light in the right eyepiece/no light in the left eyepiece/100% light in the video/photo beam path Focusing and framing down the le[...]

  • Page 33

    … the field of vision is in shadow? • Adjust interpupillary distance and ensure that it is correct (p.23). • Check pupil position (p.23). … the image will not stay in focus? • Insert eyepieces correctly (p.16). • Correct diopter adjustment exactly as described (p.28–31). … the focusing drive slides down or is difficult to turn? • [...]

  • Page 34

    34 In this section we would like to explain how you can take care of your microscope and give you a few tips on cleaning. • against moisture, vapours, acids, alkalis and caustic substances. Never keep chemicals close to your microscope. • against improper handling. Never fit other equipment connectors or dismantle optical systems and mechanical[...]

  • Page 35

    35 V arious components are made of plastic or are plastic- coated to make them pleasant to touch and handle. However , incorrect cleaning with unsuitable detergents can damage the plastic. Please observe the following do’ s and don’ts: • in an ultrasonic cleaner . The plastic may become brittle and eventually break. • with caustic or aceton[...]

  • Page 36

    Leica StereoZoom User Manual 36 Y ou are working with a high-performance precision instrument, whose quality we guarantee. The warranty covers manufacturing and materials faults, but not damage resulting from negligence or incorrect handling. If you treat your valuable optical instrument with due care, it will repay you with the decades of reliable[...]

  • Page 37

    37 37 Leica StereoZoom User Manual – Special notes M O Objective magnification M E Eyepiece magnification z Magnification changer setting N FOV Eyepiece field number . Field numbers are printed on the eyepieces: 10 × / 23 , 16 × / 16 , 20 × / 12 , 10 × / 23 B, 16 × / 14 B, 25 × / 9.5 B, 40 × / 6 B Example: M O 1.6 × objective M E 20 × /1[...]

  • Page 38

    Leica StereoZoom User Manual 38 with additional objectives 0.32 × 0.5 × 0.63 × 0.75 × 1.6 × 110mm 300mm 200mm 155mm 130mm 55mm T otal magnifi c ation Working distance Eyepieces Zoom position *4.0: S 6 40 × /6B 10 445 303 6.3 8.0 10.0 12.5 16.0 20.0 25.0 32.0 40.0 10.1 12.8 16.0 20.0 25.6 32.0 40.0 51.2 64.0 12.6 16.0 20.0 25.0 32.0 40.0 50.0 [...]

  • Page 39

    39 Leica StereoZoom User Manual 39 12.6 16.0 20.0 25.0 32.0 40.0 50.0 64.0 80.0 20.2 25.6 32.0 40.0 51.2 64.0 80.0 102.4 128.0 25.2 32.0 40.0 50.0 64.0 80.0 100.0 128.0 160.0 20.2 25.6 32.0 40.0 51.2 64.0 80.0 102.4 128.0 31.5 40.0 50.0 62.5 80.0 100.0 125.0 160.0 200.0 50.4 64.0 80.0 100.0 128.0 160.0 200.0 256.0 320.0 18.3 14.4 11.5 9.2 7.2 5.8 4[...]

  • Page 40

    40 75mm 101mm 37mm 25mm 200mm Apochromats Achromate 0.63 ×  1.6 ×  2.0 × 0.32 × T otal magnification Working distance Eyepieces Zoom position 10.0 12.5 16.0 20.0 25.0 32.0 40.0 50.0 63.0 80.0 16.0 20.0 25.6 32.0 40.0 51.2 64.0 80.0 100.8 128.0 25.0 31.3 40.0 50.0 62.5 80.0 100.0 125.0 157.5 200.0 40.0 50.0 64.0 80.0 100.0 128.0 160.[...]

  • Page 41

    Leica StereoZoom User Manual 41 with incident-light stand Dimensions, Leica S4 E, S6 E, S6 T 161 107 23 25 81 110 351 38 ° 22 134 223 278 393 ø58 ø76 46 278 393 205 144 104 113 148 205 Leica S4 E, S6 E, S6 T Dimensions in mm[...]

  • Page 42

    42 161 107 23 25 81 110 351 38 ° 22 65 134 223 278 393 ø58 ø76 46 with transmitted-light stand Dimensions, Leica S4 E, S6 E, S6 T 144 104 113 148 205 220 278 393 205 Leica S4 E, S6 E, S6 T Dimensions in mm 42 Leica StereoZoom User Manual[...]

  • Page 43

    207 107 23 25 81 110 351 38 22 134 223 278 393 ø58 ø76 171 278 393 205 144 104 113 148 205 43 with inclining focusing drive Dimensions, Leica S6 Leica StereoZoom User Manual Dimensions, Leica S6 D 120 148 25 81 110 390 60 ° ø15.8 25 26 35 ø58 ø76 46 144 104 150.4 Leica S6 Dimensions in mm Dimensions in mm Leica S6 D with incident-light stand[...]

  • Page 44

    44 Leica StereoZoom User Manual 207 107 23 25 81 110 351 38 22 65 134 223 278 393 ø58 ø76 171 278 393 205 144 104 113 148 205 220 Leica S6 D with incident-light stand Dimensions, Leica S6 D Dimensions in mm[...]

  • Page 45

    Leica StereoZoom User Manual 45 Leica StereoZoom User Manual with incident-light stand Dimensions, Leica S8 APO 207 107 23 25 116 75 351 38 22 134 223 278 393 ø58 ø76 171 278 393 205 144 104 113 148 205 Dimensions in mm Leica S8 APO[...]

  • Page 46

    46 with transmitted-light stand Dimensions, Leica S8 APO 207 107 23 25 116 75 351 38 22 65 134 223 278 393 ø58 ø76 171 144 104 113 148 205 220 278 393 205 Dimensions in mm Leica S8 APO Leica StereoZoom User Manual[...]

  • Page 47

    47 47 Leica StereoZoom User Manual Leica Microsystems is active in microscopy , sample preparation, image analysis, confocal laser technology , medical equipment and equipment for the semiconduc- tor industry . With headquarters in W etzlar , Germany , this international technology group has grown from five companies with a long tradition – Wild,[...]

  • Page 48

    Leica Microsystems – the brand for outstanding products Leica Microsystems – an international company with a strong network of customer services Australia: Gladesville, NSW T el . + 1 800 625 286 Fax +61 2 9817 8358 Austria: Vienna T el . +43 1 486 80 50 0 Fax +43 1 486 80 50 30 Canada: Richmond Hill/Ontario T e l. + 1 905 762 20 00 Fax +1 905 [...]