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Leica SP2600 manual

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Table of contents for the manual

  • Page 1

    Leica SP2600 Ultramilling attachment Supplementary instruction manual Leica SP2600 To be used only in conjunction with instruction manual Leica SM2500 . V1.2 English  07/2002 Always keep this manual near the instrument. Read carefully prior to operating the instrument.[...]

  • Page 2

    [...]

  • Page 3

    3 Leica SP2600  Ultramilling attachment The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and state- of-the-art technology as we understand it follow- ing thorough investigation in this field. We are under no obligation to update the present manual according to[...]

  • Page 4

    4 Supplementary instruction manual V 1.2  07/2002 Table of contents 1. Important information ........................................................................................................ ........ 6 1.1 Symbols in the text and their meaning ....................................................................................... ........[...]

  • Page 5

    5 Leica SP 2600  Ultramilling attachment Important information 6. Troubleshooting .............................................................................................................. ......... 44 7. Cleaning, disinfection and maintenance ............................................................................ 45 7.1 Cleaning and di[...]

  • Page 6

    6 Supplementary instruction manual V 1.2  07/2002 1. Important information 1.2 Designated use The Leica SP2600 is designated to prepare milled surfaces and thin slices of specimen materials such as plastics and metals as well as of organic materials (such as bones, teeth) in medicine, biol- ogy and industry. The Leica SP2600 ultramilling attachm[...]

  • Page 7

    7 Leica SP 2600  Ultramilling attachment 1. Important information 1.3 Information on this instruction manual Instrument type This supplementary instruction manual must be used together with the Leica SM2500 sliding microtome instruction manual describing the basic instru- ment Leica SM2500, which is needed to operate the Leica SP2600 ultramill- [...]

  • Page 8

    8 Supplementary instruction manual V 1.2  07/2002 2. Safety 2.1 Safety regulations This instrument has been built and tested in accordance with the same di- rectives and standards as the Leica SM2500. For a complete list of the directives and standards applied please refer to chapter 2.1, page 8 of the Leica SM2500 instruction manual. In order t[...]

  • Page 9

    9 Leica SP 2600  Ultramilling attachment 2. Safety 2.2 Built-in safety devices The instrument is equipped with the following safety devices: Plexiglass protective cover  The miller is equipped with a protective cover made of plexiglass ( 1 ). When the cover is moved to the upper limit position ( 1, Fig. 2.1 ) the miller is automatically block[...]

  • Page 10

    10 Supplementary instruction manual V 1.2  07/2002 Be sure to read and comply with the safety instructions, warnings and cau- tions in this chapter as well as in chapter 2 of the Leica SM2500 instruc- tion manual, even if you are already fa- miliar with the operation and use of other Leica products. Safety instructions - general The Leica SP 260[...]

  • Page 11

    11 Leica SP2600  Ultramilling attachment Safety instructions - transport and installation Caution! Only the vacuum cleaner offered by Leica or other vacuum cleaner brands rated at 230 V/ 50 Hz and a maximum power draw of 4 A can be connected directly to the SM2500 control unit (see page 17), and this only in countries where the mains supply is 2[...]

  • Page 12

    12 Supplementary instruction manual V 1.2  07/2002 Safety instructions - cleaning disinfection and maintenance Only authorized service technicians may access the internal components of the instrument for service and repair! Cleaning detergents appropriate for the ultramilling attachment. Varnished surfaces:  mild laboratory detergents (slight[...]

  • Page 13

    13 Leica SP 2600  Ultramilling attachment 3. Technical Data Ultramilling attachment Milling spindle (special design) ......................................................................................... type T SAV 60 x 160 Motor, rotation speed selectable in 100-rpm steps ............................................................ 500 to 3.[...]

  • Page 14

    14 Supplementary instruction manual V 1.2  07/2002 4. Installation 4.1 Overview - Leica SP2600 Ultramilling attachment / basic instrument Leica SM2500 - instrument parts / functions 1. Leica SM2500 - basic instrument (sliding microtome) 1.1 Leica SP2600 - ultramilling attachment 1.2 Carrier for ultramilling attachment SP2600 (when not in use) 1.[...]

  • Page 15

    15 Leica SP2600  Ultramilling attachment 4. Installation 4.2 Leica SP2600 standard delivery Standard delivery includes: 1 Basic instrument Leica SP2600 (Ultramilling attachment) 1 Calibrating device with dial gauge for miller adjustment 3 Holders, each with two springs, for pre- and finishing millers, one holder delivered attached to the instrum[...]

  • Page 16

    4.5 Installing the Leica SP2600 ultramilling attachment  Place the carrier ( 1, Fig. 4.2 ) for the ultramilling attachment ( 3, Fig. 4.3 ) on the rear part of the Leica SM2500 microtome fitting the two pins of the carrier into the corresponding bores in the Leica SM2500 microtome.  Lower the bearing blocks ( 2 ) of the microtome as far as the[...]

  • Page 17

    4. Installation 4.6 Connecting the vacuum cleaner Milling dust is extracted by a vacuum cleaner, through a nozzle ( 1 ) located in the milling area. 230 V / 50 Hz Vacuum cleaners with a power draw of maximum 4 A can be connected directly to the SM2500 control unit and thus be switched on/off via the vacuum cleaner button on the SM2500 con- trol uni[...]

  • Page 18

    18 Supplementary instruction manual V 1.2  07/2002 4.7 Installing additional accessories  Install further accessories as described in chapters 4.5.2 and 4.5.3, pages 18 and 19 of the Leica SM2500 instruction manual.  Connect the foot switch. The foot switch must be connected - otherwise the instrument will not be operational. --> For de[...]

  • Page 19

    19 Leica SP 2600  Ultramilling attachment 5.1 Overview of chapter 5 Operation Prior to working with the Leica SP2600 ultramilling attachment, be sure to read the following instruction manuals:  The entire Leica SM2500 instruction manual except chapters 5.5.6 through 5.7.  This supplementary instruction manual Leica SP2600. Chapter 5 [...]

  • Page 20

    20 Supplementary instruction manual V 1.2  07/2002 5.2 Adjusting the pre-millers in the calibrating device Preparatory steps  Connect mains power supply ( 20 ) to miller cali- bration device ( 18 ).  Connect mains power supply ( 20 ) to mains: the light bulb is switched on.  Place magnifier ( 16 ) onto calibration device.  Loosen reg[...]

  • Page 21

    21 Leica SP2600  Ultramilling attachment Adjusting the miller The miller ( 3 ) is adjusted in the holder ( 2 ) along three different axis (x, y and z - see Fig. 5.3 , left). Adjustment along the x-axis is done outside the calibration device ( 18, Fig. 5.1 ). For adjustment along the z- and y-axis, the miller holder ( 2 ) with miller ( 3 ) has to[...]

  • Page 22

    22 Supplementary instruction manual V 1.2  07/2002 Adjustment along the z-axis 1. Pull lever ( 12 ) at the left of calibration device ( 18 ) forward as far as it will go, thus moving contact plate ( 17 ) backwards to its rear limit position. This prevents the contact plate from hitting the milling edge during the adjustment procedure. 2. Once mi[...]

  • Page 23

    23 Leica SP2600  Ultramilling attachment 6. Insert the size-5 Allen key into regulating screw ( 4 ) insert size-2.5 Allen key into regu- lating screw ( 11 ) ( Fig. 5.7 ). The arrows in Fig. 5.7 indicate clock- wise sense of rotation. 7. Tighten/loosen screws ( 11 ) and ( 4 ) alternately to adjust miller ( 3 ) along the z-axis. The miller edge is[...]

  • Page 24

    24 Supplementary instruction manual V 1.2  07/2002 Adjustment along the y-axis 1. Select reference value 1. The reference value will be needed later on to adjust the finishing miller to the same milling level as the pre- miller. Any reference value can be selected. Experience, however, shows that 1 is the best value for new millers ([...]

  • Page 25

    25 Leica SP2600  Ultramilling attachment 5.3 Adjusting the finishing millers in the calibrating device  Carry out preparatory steps as per chapter 5.2, page 20 (Adjusting the pre-millers in the cali- brating device). Twisted or deformed springs for the miller holders must be exchanged!  Insert spring and finishing miller as described[...]

  • Page 26

    26 Supplementary instruction manual V 1.2  07/2002 Adjusting the finishing miller Just like the pre-miller, the finishing miller is ad- justed in the miller holder along three different axis (see page 21). Adjustment along the x-axis As described for the pre-miller - see page 21. Adjustment along the z-axis  Carry out steps 1 - 4 as described[...]

  • Page 27

    27 Leica SP2600  Ultramilling attachment Fig. 5.12 4 11 3 7 6 Insert size-5 Allen key into regulating screw ( 4 ) insert size-2.5 Allen key into regulating screw ( 11 ) (Fig. 5.12 ) . The arrows in Fig. 5.12 indicate clock- wise sense of rotation. 7 Tighten/loosen screws ( 11 ) and ( 4 ) alternately to adjust miller ( 3 ) along the z-axis. The m[...]

  • Page 28

    28 Supplementary instruction manual V 1.2  07/2002 Adjustment along the y-axis 1 Select the same reference value as was set for the pre-miller. Pre-miller and finishing miller used for milling a specific sample must be ad- justed using the same reference value. This ensures that both milling edges hit the sample at exactly the same height. Minor[...]

  • Page 29

    29 Leica SP 2600  Ultramilling attachment 5.4 Specimen holder systems - how to combine individual components 5. Operation Configurations: 1. Base plate with dovetail guide (0383 09422) and holder for round specimens (0383 08992) = order no. 0383 08993 2. Base plate with dovetail guide (0383 09422) and specimen clamp 44 x 58 mm (0383 09415) with [...]

  • Page 30

    30 Supplementary instruction manual V 1.2  07/2002 5.5 Clamping specimen For a detailed overview of the clamping systems available, please refer to chapter, 5.4, page 29. 5.5.1 Installing the base plates in the basic instrument For detailed instructions on how to install the base plates in the basic instrument, please refer to chapters 5.5/5.5.1[...]

  • Page 31

    31 Leica SP2600  Ultramilling attachment 5.5.3 Clamping the vacuum stages on/in the base plates Clamping the vacuum stages in the base plate with vise (Fig. 5.15)  Fasten the vacuum stage on the vacuum stage adapter for the base plate with vise (with 4 Allen screws ( 1 ) / Allen key size 4). The 4 size-four Allen screws are part of the Vac[...]

  • Page 32

    32 Supplementary instruction manual V 1.2  07/2002 5. Operation 5.5.4 Connecting the vacuum pump / placing specimens onto the vacuum stages IMPORTANT: Prior to first use, every vacuum table surface must be milled once, just like a specimen surface. This ensures that the surface of the specimens milled on the table later on will be perfectly even[...]

  • Page 33

    33 Leica SP2600  Ultramilling attachment Placing specimens onto vacuum stage for 30 - 50-mm lids  Fasten a lid suited to the size of the specimen to be milled onto the vacuum stage (slotted screws are part of standard delivery of vacuum stage lids - use slotted screwdriver).  Place the specimen ( 1 ) onto the sintered sur- face ( 2 ) of th[...]

  • Page 34

    34 Supplementary instruction manual V 1.2  07/2002 5.6 Preparatory steps prior to the milling procedure For a detailed description of all control elements located on the control unit, please refer to chapters 5.3 (Overview - control panel) and 5.4 (Description of each individual control element) of the Leica SM2 500 instruction manua[...]

  • Page 35

    35 Leica SP 2600  Ultramilling attachment  Insert the specimen (see chapters 5.5.2 and 5.5.4, pages 30 and 33 of this manual) or - if you need to mill a vacuum table prior to first use - see chapter 5.5.3, page 31 of this manual). Wear protective gloves when working with potentially infectious specimen material!  Move the specimen sledge u[...]

  • Page 36

    36 Supplementary instruction manual V 1.2  07/2002 5.7 Pre-milling  Select automatic mode of operation  AUTO (button ( 5 ) - chapter 5.4.3, page 26 Leica SM 2500 instruction manual). In AUTO mode, set the milling parameters for the pre-milling procedure:  Select STOP or PHOTO position (buttons ( 23 ) or ( 26 ) chapter 5.4.8, pa[...]

  • Page 37

    37 Leica SP 2600  Ultramilling attachment Recommended milling thickness settings for pre-milling  10 µm - keep milling until surface is completely level.  5 µm - three entire sledge strokes (milling and return stroke).  3 µm - three entire sledge strokes (milling and return stroke). Do not exceed a milling thickness setting of 5 µm [...]

  • Page 38

    38 Supplementary instruction manual V 1.2  07/2002 5.7.1 Perspective view of the pre-milling procedure 5. Operation Pre-milling procedure The milling spindle carrying the pre-miller rotates clockwise (circular arrow ( 1 )) while the specimen sledge passes underneath the miller in the direction of arrow ( 2 ). The pre-miller leaves a serrated sur[...]

  • Page 39

    39 Leica SP 2600  Ultramilling attachment 5. Operation Ending the pre-milling procedure  Press RUN STOP or RUN ENABLE to stop the sledge (buttons ( 29 ), ( 30 ) - Chapter 5.4.10, page 33 - Leica SM2500 manual) - or, when working in  PROG  mode of operation, wait for the programmed number of strokes to be finished (see chapter 5.4.3, pag[...]

  • Page 40

    40 Supplementary instruction manual V 1.2  07/2002 5.8 Finishing milling  Attach the miller holder containing the finishing miller to the milling spindle (Allen screws / Allen key size 5) - see Fig. 5.21, page 34). Always slide the plexiglass cover to the upper limit position prior to attaching the miller holders to the milling spindle!  B[...]

  • Page 41

    41 Leica SP 2600  Ultramilling attachment Recommended sledge speed settings for finishing milling  Milling stroke: 0.5 mm/s to 1.0 mm/s  Return stroke: any speed Recommended rotational speed of miller for finishing milling  < 2.000 rpm Recommended mode of operation for finishing milling  Select continuous mode of operation. [...]

  • Page 42

    42 Supplementary instruction manual V 1.2  07/2002 5.8.1 Perspective view of the finishing milling procedure 5. Operation Finishing milling procedure The milling spindle carrying the finishing miller rotates clockwise (circular arrow ( 1 )) while the specimen sledge passes underneath the miller in the di- rection of arrow ( 2 ). The serrated sur[...]

  • Page 43

    43 Leica SP 2600  Ultramilling attachment Ending the finishing milling procedure  Press RUN STOP or RUN ENABLE to stop the sledge (buttons ( 29 ), ( 30 ) - chapter 5.4.10, page 33 - Leica SM2500 manual) - or, when working in  PROG  mode of operation, wait for the programmed number of strokes to be finished (see chapter 5.4.3, page 26, L[...]

  • Page 44

    44 Supplementary instruction manual V 1.2  07/2002 6. Troubleshooting 6. Troubleshooting For all instructions on error detection and trouble shooting, please refer to chapter 6, page 64 of the Leica SM2 500 instruction manual .[...]

  • Page 45

    45 Leica SP 2600  Ultramilling attachment 7. Cleaning, disinfection and maintenance 7.1 Cleaning and disinfection Only authorized service technicians may access the internal com- ponents of the instrument for service and repair! Cleaning detergents appropriate for the ultramilling attachment - varnished surfaces:  mild laboratory detergents ([...]

  • Page 46

    46 Supplementary instruction manual V 1.2  07/2002 7. Cleaning, disinfection and maintenance 7.2 Maintenance Only authorized service technicians may access the internal com- ponents of the instrument for service and repair! No liquids may enter the interior of the instrument during mainte- nance!  Carry out the maintenance steps to be done by[...]

  • Page 47

    47 Leica SP 2600  Ultramilling attachment 7.2.2 Replacing the light bulb in the calibration device  Remove base cover ( 2 ) of calibration device ( 1 ) (unscrew 4 slotted screws ( 3 )).  Loosen light bulb holder ( 5 ) (metal bridge with lamp socket) (unscrew 2 slotted screws ( 4 )).  Take replacement light bulb ( 8 ) out of sponge rubbe[...]

  • Page 48

    48 Supplementary instruction manual V 1.2  07/2002 8. Overview - accessories 8.1 Miller holders and millers Miller holder Holder for pre- and finishing miller Pre-miller  Pre-miller D - with diamond edge, cutting angle -1°, clearance angle +3° Finishing miller  Finishing miller D - with diamond edge, cutting angle +3°, clearance angle +[...]

  • Page 49

    49 Leica SP 2600  Ultramilling attachment 8.5 Vacuum stages and matching lids / perforated specimen holders  Vacuum stage for Leica SP2600 - basic body for lids 30 - 50 mm  Lid, 30 mm, for Leica SP2600 vacuum stage  Lid, 40 mm, for Leica SP2600 vacuum stage  Lid, 50 mm, for Leica SP2600 vacuum stage  Vacuum stage for Leica SP2600 [...]

  • Page 50

    50 Supplementary instruction manual V 1.2  07/2002 8.7 Additional accessories Vacuum cleaner and vacuum cleaner accessories  Vacuum cleaner Fakir S20, with connection hose 230 V / 50 Hz only  Set of vacuum cleaner bags for Fakir S 20 Illumination  Halogen spotlight illumination 230 V/50 Hz only 8 Overview - accessories[...]

  • Page 51

    51 Leica SP 2600  Ultramilling attachment 9. Warranty and service For all information on warranty and service, please refer to chapter 9, pages 74 - 76 of the Leica SM2500 instruction manual. 9. Warranty and service[...]