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Olympus FV300 manual

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Table of contents for the manual

  • Page 1

    CONFOCAL LASER SCANNING BIOLOGICAL MICROSCOPES FV300 FLUOVIEW[...]

  • Page 2

    1 FV300 CONFOCAL LASER SCANNING MICROSCO PERFORMANCE FOR THE PERSONAL USER The FV300 is the ideal choice of laser scanning microscopes for personal users. Its optical system is fully integrated, from scanner to microscope, and not only delivers outstanding optical sectioning, but also ensures the easy, flexible expandability required for any future[...]

  • Page 3

    2 OPE q e r t y u i o !0 !1 !2 w q Optical fiber for laser introduction w Beam collimator e Polarizer r Dichromatic mirror t Excitation dichromatic mirror y XY galvanometer mirror scanners u Pupil lens i Collector Lens o Pinhole turret !0 Emission beam splitter slider !1 Barrier filter slider !2 Photo multiplier[...]

  • Page 4

    Preset the conditions for image acquisition and loading Storage of Acquisition Settings enables immediate, one-touch recall of all the relevant experimental settings and conditions. Adding new conditions or altering existing ones is quick and easy. AOTF: flexible control of the laser intensity to meet the specific demands (optional) The laser expos[...]

  • Page 5

    Image tool bar X-Y-Z scanning operations and time- lapse observations both produce multiple images, which can be displayed in sequence simply by clicking the sequential mode button. Channel selection and image zooming are also available on the same menu. 4 Thumbnail display Data stored in the gallery window are displayed as thumbnails for easy view[...]

  • Page 6

    5 X -t Slant Line-t FreeLine-t Y -t Y-Z-t X-Y-t ZoomIn-t X-Z-t X-Y-t X-Y-Z-t ZoomIn-Z-t Patch clamp BX61WI fixed stage upright microscope+translation stage Image acquisition ROI designation Intensity versus time measurement Calcium sparks in isolated cardiac myocyte Dr. Sandor Gyorke Texas Technical University X -t X -t Time Course Using different [...]

  • Page 7

    6 The 440nm diode laser can be added for CFP/YFP FRET imaging. A 440nm diode laser is optionally available for CFP/YFP imaging. The 440nm laser line ideally excites CFP, with minimal disturbance to YFP, and is therefore suitable for CFP/YFP FRET experiments. The high performance LSM objectives, PLAPO40XWLSM and PLAPO60XWLSM, are precisely corrected[...]

  • Page 8

    7 PAPP for FRAP Application PAPP: Programmable Acquisition Protocol Processor Easy, reliable flow of experiments for fluorescence recovery after photobleaching. AOTF and PAPP function for effective FRAP (Fluorescence Recovery After Photobleaching) Fluorescence recovery after photobleaching can be analyzed on any designated area by means of the AOTF[...]

  • Page 9

    8 Multi-point time lapse system High-magnification multi-point time lapse observation of living cells. Wide-ranging specimen observations for improved experiment throughput Use of a motorized XY stage allows the analysis of time lapse changes in many points scattered over a wide area. The system is therefore effective for work with thick specimens,[...]

  • Page 10

    9 Tile display Topographic projection Height of 3D structure indicated by color. X-Z Y-Z X-Y-Z X -Z Y -Z Using multiple 3D images to obtain accurate 3D structure analysis. 3D Imaging Easy Z axis operation and setting The upper and lower limit of Z scanning can be specified interactively by actually scanning the sample or by direct input of the nume[...]

  • Page 11

    10 Link with patch clamping data • With PCs linked through a LAN, Physiolink can synchronize electrophysiological and confocal image data simultaneously. • With the same time stamp recorded in the two PCs, it is possible to access and analyze an image and its patch clamping data corresponding to the Physiolink software time scale. • The patch[...]

  • Page 12

    11 Lucifer Yellow: retina ganglion cell TexasRed: dopamine-operated amacrine cell Prof. Shigetada Nakanishi Dept. of Biological Sciences, Kyoto Univ. Faculty of Medicine Applications Gallery Morphology Structure of PtK2 cell Nucleus: DAPI (Blue) Actin: FITC (Green) Mitochondria: Mito Tracker (Red) Microtubules: Cy5 (White) Neuron Rat tongue taste b[...]

  • Page 13

    12 Fluorescent Proteins C elegans expressing beta-integrin fused to GFP Dr. Xioping Xhu and Dr. John Plenefisch University of Toledo, Dept. of Biology GFP-labeling of Drosophila adults Expression of DsRed in a zebrafish embryo Extended focus image of 5µmx30 slice Pr. Yasuhiro Kamei, Pr. Shunsuke Yuba Institute for Molecular and Cellular Biology Os[...]

  • Page 14

    Fluorescence Dyes and Filters 300 400 500 600 700 BA530RIF BA550RIF BA650RIF BA430-460 BA465-495 BA480-495 BA470-520 BA510-540 BA520-550 BA505-550 BA505-525 BA480-510 BA535-565 BA540-590 BA560-600 BA585-615 BA510IF BA560IF BA565IF BA585IF BA590 BA610IF BA625IF BA660IF BA585-640 440 LD 405 LD 458 Multi Argon 488 Multi Argon 515 Multi Argon 543 Green[...]

  • Page 15

    Objectives for fixed stage upright microscopes (using WI-UCD, WI-DICTHRA) 14 Item Specifications Laser light Visible light laser source Select from the following laser, to mounted on laser combiner source Multi-line Ar laser (458nm, 488nm, 515nm, Total 40mW), Ar laser (488nm,10mW), Kr laser (568nm, 10mW), HeNe (G) laser (543nm,1mW), HeNe (R) laser [...]

  • Page 16

    ● All brands are trademarks or registered trademarks of their respective owners. ● Monitor images are simulated. Specifications are subject to change without any obligation on the part of the manufacturer. This catalog is printed by enviromentally-friendly waterless printing system with soy ink. ISO 900 1 Certification Design and production adh[...]