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Olympus Microscope manual

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Table of contents for the manual

  • Page 1

    MICROSCOPE COMPONENTS GUIDE Choosing The Ideal UIS2 Optics Components For Y our Equipment 2006-11[...]

  • Page 2

    The wide range of Olympus components introduced here allows users in such diverse fields as resear ch, inspection and production to take advantage of the quality , flexibility and outstanding optical performance of the UIS2 Optical System. That's why installing Olympus microscope components is, quite simply , the right choice for your equipmen[...]

  • Page 3

    CONTENTS 2 EYEPIECES/FILAR MICROMETER EYEPIECE --------------------------------- 33 Widefield eyepieces WHN10x, WHN10 x-H, CROSSWHN10x, WH15 x --------------------- 33 Super widefield eyepieces SWH10x-H, MICROSWH10 x, CROSSSWH10x ------------------------------ 33 Filar micrometer eyepiece U-OSM --------------------------------------- 33 REVOLVING N[...]

  • Page 4

    light between the objective lens and tube lens, allowing the creation of user -specific or task- specific optical systems. T o establish real flexibility with such a system, it is necessary to eliminate the occurrence of coma aberration. *In UIS2/UIS objective lenses, the parfocal distance is designed at 45mm and the focal length of the tube lens i[...]

  • Page 5

    4. Lightening Weight has been r educed to appr oximately 2/3 that of conventional products by using an aluminum objective lens barrel cover . This has the effect of lightening the load on the devices at objective lens up/down, suppressing vibrations by lowering the inertial force at objective lens switching, etc. (MPLFLN series, LMPLFLN series) 5. [...]

  • Page 6

    5 SYSTEM DIAGRAM 5 6 SHUTTER SHUTTER 5 6 SHUTTER U-AN U-AN360-3 U-AN360IR LG-SF LG-PS2 U-LH100HGAPO U-LH100HG U-LH75XEAPO U-LH100-3 U-LH100L-3 U-LH100IR Super widefield trinocular observation tubes Widefield trinocular observation tubes U-TLU U-TLUIR U-25ND6, U-25ND25, U-25LBD, U-25IF550, U-25L42, U-25FR, U-BP1100IR, U-BP1200IR U-PO3 U-POIR SZ2-STU[...]

  • Page 7

    6 SYSTEM DIAGRAM MOTORIZED UNIT SYSTEM DIAGRAM BXFM-A SYSTEM DIAGRAM See manual U-D5BDREMC U-D6REMC U-FWO BX-RFAA U-AN360RAF U-AFA1M BX-RLAA U-LH100-3 U-LH100L-3 U-FWR Intermediate tubes (Refer to pages 31-32) Observation tubes (Refer to pages 29-30) U-DICRHC U-DICR U-DICRH U-AN360-3 U-25ND6, U-25ND25 U-25LBD U-25IF550 U-25L42 U-25FR U-PO3 U-AN U-H[...]

  • Page 8

    7 UIS2/UIS OBJECTIVE LENSES MPL (Plan) FLN-100 BD ■ Objective lens series list ■ Meaning of abbreviations shown on objective lens ■ Objective lens notation ■ Features of objective lens series None: Brightfield BD: Brightfield/darkfield BDP: Brightfield/darkfield/ polarizing IR: IR LCD: LCD None: UIS N: UIS2 Number: Objective lens magnificat[...]

  • Page 9

    UIS2 OBJECTIVE LENSES 8 M Plan SemiApochr omat MPLFLN series Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. The lineup consists of 7 objectiv e lenses ranging from 1.25x to 100x, and secures a W.D. of 1mm or longer. Since the exit pupil position of the 5x-100x objective lenses is standardized, the posit[...]

  • Page 10

    UIS2 OBJECTIVE LENSES 9 Long WD M Plan SemiApochr omat (WD: Working Distance) LMPLFLN series Long working distance Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. Suitable with samples having a height difference and in preventing collision, as the working distance is long. Also, since the exit pupil posi[...]

  • Page 11

    10 UIS2 OBJECTIVE LENSES Unit: mm M Plan Achr omat MPLN series Plan Achromat objective lenses providing excellent image flatness up to F.N. 22. MPLN 5x 0.10 20.0 36 64 50 4.4 98 MPLN 10x 0.25 10.6 18 80 100 2.2 18 MPLN 20x 0.40 1.3 9 111 200 1.1 6.1 MPLN 50x 0.75 0.38 3.6 113 500 0.44 1.4 MPLN 100x 0.90 0.21 1.8 116 1000 0.22 0.73 Numerical Apertur[...]

  • Page 12

    UIS2 OBJECTIVE LENSES 11 LCD Long WD M Plan SemiApochr omat LCPLFLN-LCD series Perfect objective lens series for observation of LCD panels and other samples through a glass substrate. Aberration correction matched to the glass thickness is accomplished using a correction ring. Objective lens LCPLFLN20 xLCD LCPLFLN50xLCD LCPLFLN100xLCD Corresponding[...]

  • Page 13

    UIS OBJECTIVE LENSES 12 Unit: mm ø 5.6 ø 16.5 ø 20 ø 28 WD=0.9 34.7 38.7 40.1 (44.1) 45 4.5 ø 20.32 36.8 40.7 41.7 (44.7) WD=0.3 45 4.5 ø 8 ø 16.6 ø 20.2 ø 28 ø 20.32 ø 5.3 ø 16.5 ø 20.2 40.7 41.5 (44.65) WD =0.35 45 4.5 ø 20.32 ø 28 36.8 MPlanApo20x MPlanApo100x MPlanApo50x M Plan Apochr omat MPlanApo series Highest class Plan Apoch[...]

  • Page 14

    13 UIS OBJECTIVE LENSES IR Long WD M Plan SemiApochr omat/IR M Plan SemiApochr omat LMPlan-IR series/MPlan-IR IR objective lenses which compensate for aberrations from visible to near infrared light. Ideal for the observations of semiconductor interiors and the back surface of a chip package as well as CSP bump inspection. 34.3 30.3 22.5 ø 21 ø 2[...]

  • Page 15

    UIS2 OBJECTIVE LENSES 14 M Plan SemiApochr omat BD (BD:Brightfield/Darkfield) MPLFLN-BD series Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. The series secures a W.D. of 1mm or longer. Since the exit pupil position of the 5x-150x objective lenses is standardized, the position of the DIC prism does not [...]

  • Page 16

    15 UIS2 OBJECTIVE LENSES Screw: W26x0.706 Unit: mm MPLFLN 5xBDP 0.15 12.0 36 95.5 50 4.4 59 50 5.3 59 MPLFLN 10xBDP 0.25 6.5 18 83.3 100 2.2 18 100 2.7 18 MPLFLN 20xBDP 0.40 3.0 9 88.5 200 1.1 6.1 200 1.3 6.1 MPLFLN 50xBDP 0.75 1.0 3.6 100.5 500 0.44 1.4 500 0.53 1.4 MPLFLN 100xBDP 0.90 1.0 1.8 101.5 1000 0.22 0.73 1000 0.27 0.73 Numerical Aperture[...]

  • Page 17

    UIS2 OBJECTIVE LENSES 16 Long WD M Plan SemiApochr omat BD LMPLFLN-BD series Long working distance Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. Suitable with samples having a height difference and in preventing collision, as the working distance is long. Also, since the exit pupil position of the 5x-1[...]

  • Page 18

    UIS2 OBJECTIVE LENSES 17 M Plan Achr omat BD MPLN-BD series Plan Achromat objective lenses providing excellent image flatness up to F.N. 22. MPLN 5xBD 0.10 12.0 36 137 50 4.4 98 MPLN 10xBD 0.25 6.5 18 155 100 2.2 18 MPLN 20xBD 0.40 1.3 9 162 200 1.1 6.1 MPLN 50xBD 0.75 0.38 3.6 157 500 0.44 1.4 MPLN 100xBD 0.90 0.21 1.8 160 1000 0.22 0.73 Numerical[...]

  • Page 19

    18 UIS OBJECTIVE LENSES ø 26 41 42.5 43.2 (44.69) 4.5 WD=0.31 ø 10 ø 23 ø 27 ø 29 ø 32 45 MPlanApo100xBD M Plan Apochr omat BD MPlanApo-BD Highest class Plan Apochromat objective lens that maximize performance in brightfield and darkfield observations. All aberrations are corrected at the highest level, while providing high N.A. MPlanApo100xB[...]

  • Page 20

    BXFM frame BXFM-F Widely used system that allows use in combination with fiber illumination, motorized revolving nosepiece and telan lens unit. Can easily be integrated into other equipment. Attach to the equipment by rear bolt mounting screw or pillar mounting hole. 19 MICROSCOPE SYSTEM BXFM Weight: 1.9kg Unit: mm 98 110 80 58.5 0.5 (13) (13) 55 2[...]

  • Page 21

    MICROSCOPE SYSTEM BXFM 20 BXFM BXFM-F+BXFM-ILH+BXFM-ILHSPU Accommodates the reflected light brightfield/darkfield and fluorescence illuminators. Weight: 3.2kg Unit: mm 11 Light axis Light axis 165 130 83 45 40 72 23 7 3.5 Holder mounting position Pillar axis Revolving nosepiece mounting position Objective lens mounting position Stroke Specimen posi[...]

  • Page 22

    MICROSCOPE SYSTEM BXFM 21 ø 32 169 (Stroke) 19-49 124 84 208 290 20 40 45 106 187 92.5 Specimen surface BXFM-S combination sample BXFM-F+BXFM-ILHS+TR30-2+U-KMAS+U-LH100L-3 Weight: 5.5kg (exclude objective lens) Unit: mm BXFM-S BXFM-F+BXFM-ILHS Compact focusing unit suitable for building into existing equipment. Weight: 2.4kg Unit: mm 20 Stroke 7 2[...]

  • Page 23

    MICROSCOPE SYSTEM BXFM 22 U-ST Compact stand Weight: 5kg Unit: mm Weight: 1.8kg 106 73 143 88 ø 110 133 ø 32 49 133 20 35 ø 40 130 503 ø 25 Ø 32 214~435 622 350 300 180 º 90 50.5 30 º 250 300 Stands A wide variety of stands are available to suit different applications and purposes. Item Specifications 1 Diameter of focusing arm or ø 32mm fi[...]

  • Page 24

    ILLUMINA TION UNITS 23 Universal r eflected light illuminator BX-URA2 Suitable for observations ranging from brightfield to fluorescence. Six mirror units can be attached to this reflected light illuminator simultaneously. Reflected light illuminator for BF/DF BX-RLA2 ND filters are linked when exchanging between brightfield and darkfield. Unit nam[...]

  • Page 25

    ILLUMINA TION UNITS 24 170 (Distance to the light axis) Convex section(2-4) for positioning 3.5 Less than R6 21 82 (Revolving nosepiece relief dimension) 5 5.5 (6) (11) 2.5 52 (Revolving nosepiece relief dimension) 100 ( ± 0.1) 62 ( ± 0.1) 37 ± 0.1 (45 ° location face) Location face Location face 2 45 ° ( ± 10') 4-M5 depth12 or more 36 ([...]

  • Page 26

    LAMP HOUSING & ACCESSORIES 25 Reflected light illuminator for BF U-KMAS Very compact reflected light illuminator with reduced depth. Weight: 1.2kg Unit: mm ø 75 88 198 155 21 64 6 21 Unit name Description Weight (g) U-25LBD LBD filter slider 20 U-25IF550 IF550 filter slider 20 U-25ND6 ND filter 20 U-25ND25 ND filter 20 U-25FR Frost filter slid[...]

  • Page 27

    LAMP HOUSING & ACCESSORIES 26 Lamp housings Various different lamp housings are available, for use with different light sources: choose to suit the intended purpose. Unit: mm U-LH100HGAPO 100W mercury apo lamp housing U-LH100HG 100W mercury lamp housing U-LH75XEAPO 75W xenon apo lamp housing U-LH100-3/U-LH100IR/U-LH100L-3 100W halogen lamp hous[...]

  • Page 28

    LAMP HOUSING & ACCESSORIES 27 31.5 (dimensions for installation) Darkfield light excluding tube which is built into the BX-URA2. (42.5) 21 37.5 ø 59 ø 75 43 Lamp housing accessories For the 100W halogen lamp, the external power supply TH4-100/200 with an intensity adjustment switch and an ON/OFF switch, both are located close to the operator&[...]

  • Page 29

    LAMP HOUSING & ACCESSORIES 28 Unit: mm Unit: mm Weight: 1.6kg Weight: 210g LG-PS2* Light source LG-SF Light guide U-DULHA Double lamp house adapter 8 76 235 251 10 10 126 86 130 ø 15 (Light guide mounting position) Groove: Width3, Depth1 10 ø 10.1 ø 13 ø 12 ø 25 ø 15 30 31 61 25 20 1,000 *The types of model varies by country in use. 202 1[...]

  • Page 30

    OBSERV A TION TUBES 29 Weight: 350g ø 60 57.6 • For attachable video camera adapters, refer to video camera adapters system diagram page (pages 5-6). U-TLU Single port tube with lens U-TLUIR Single port tube with lens for IR Widefield trinocular observation tubes Trinocular observation tubes with widefield of view. Compatible with F.N. 22. 33.5 [...]

  • Page 31

    30 OBSERV A TION TUBES 117.223 61.981 139.400 88.000 2.837 70.330 149.081 20 ° 0' 0" 42 ° 0' 0" 95.929 101.929 318.527 328.338 337.718 72.200 Super widefield trinocular observation tubes Trinocular observation tubes with super widefield of view. Compatible with F.N. 26.5. 55.6 79.6 180.8 49.9 68.6 98.8 173.8 220.8 82.3* 63.1 1[...]

  • Page 32

    Intermediate tubes Various accessories for various observation need. 31 INTERMEDIA TE TUBES & ACCESSORIES 45 42 ø 75 ø 70 ø 138 96 42 Unit: mm Weight: 1.3kg U-ECA Magnification changer 2x Provides 1x and 2x intermediate magnifications. Weight: 1.3kg U-CA Magnification changer Provides 1x, 1.2x, 1.6x and 2x intermediate magnifications. Weight[...]

  • Page 33

    INTERMEDIA TE TUBES & ACCESSORIES 32 Weight: 1kg U-DP Dual port Use this intermediate tube to divide the light path. Weight: 500g U-DP1xC Dual port 1x Combine with U-DP to obtain a 1x image. Weight: approximately 500g U-EPA2 Eyepoint adjuster Raises eyepoint by 30mm. Weight: 1.2kg U-APT Arrow pointer Projects an arrow into the field of view. Un[...]

  • Page 34

    EYEPIECES/FILAR MICROMETER EYEPIECE 33 Eyepieces Eyepieces for UIS2 optical system. Filar micr ometer eyepiece U-OSM Used for precise measurement in the field of view. WHN10x Widefield eyepiece WHN10x-H CROSSWHN10x Widefield eyepieces WH15x Widefield eyepiece SWH10x-H MICROSWH10x CROSSSWH10x Super widefield eyepieces (25) 18.7 43.7 29.6 ø 36.5 ø [...]

  • Page 35

    REVOL VING NOSEPIECES U-P4RE Centerable quadruple revolving nosepiece with slider slot for DIC U-P6RE Centerable sextuple revolving nosepiece with slider slot for DIC Weight: 1kg Weight: 1kg 104 (114.4) 38 40 48.2 76.4 ø 102.4 116.5 40 47.2 (125.6) 38 87.6 ø 116.5 34 116.5 40 ø 116.5 (125.6) 38 87.6 47.2 Revolving nosepieces for BF objective len[...]

  • Page 36

    REVOL VING NOSEPIECES JAPAN 2A00002 JAPAN U-D6BDRE U-D6BDRE ø 102.4 40 48.2 34.8 76.4 104 (111.2) 40 48.2 ø 102.4 38 76.4 (114.4) 104 116.5 40 Ø 116.5 (125.6) 38 87.6 47.2 U-D6BDRE W26 ✕ 0.706 W20.32 ✕ 0.706 (4) 4 8 ø 28.2 +0.2 0 ø 30 35 Revolving nosepieces for BF/DF objective lenses Choose from following 3 types. Use of adapter to mount [...]

  • Page 37

    36 2/3" CCD 1/2" CCD Field of view (F.N.) Projection area Practical field of view (mm) = Projection area (Field Number) Objective lens magnifications 2/3" CCD 1/2" CCD 1/3" CCD U-TV1x-2 1x 11 8 6 U-TV0.63xC 0.63x 17.5 12.7 9.5 U-TV0.5xC-3 0.5 x 22 16 12 U-TV0.35xC-2 0.35x — 22 17.1 U-TV0.25xC 0.25x —— 24 ]Video camera[...]

  • Page 38

    Video camera port This port can be attached directly to the trinocular observation tube as well as to the single port tube with lens. VIDEO CAMERA ADAPTERS 37 U-TV1x-2 Video port 1x 22 ø 60 28 ø 64 Video camera mount adapters Allows attachment to video cameras with C, Bayonet, Sony and F mounts. Use with the U-TV1x-2. Focus by amount of screwing [...]

  • Page 39

    MOTORIZED UNITS 38 (169) 84 40 45 41 87 (487) 400 64.8 81.5 211.5 86.2 115 80 108 11.8 12 107 Illuminator cable length: 1.800mm Weight: 5.5kg(exclude objective lens) Illuminator cable length: 1.800mm Weight: 4.3kg BX-RLAA+U-D6REMC+U-LH100-3 Motorized BF/DF reflected light illuminator+motorized Nomarski DIC sextuple revolving nosepiece+100W halogen [...]

  • Page 40

    MOTORIZED UNITS 39 68.3 190.5 115 U-D5BDREMC Motorized quintuple BD revolving nosepiece with slider slot for DIC U-D6REMC Motorized sextuple revolving nosepiece with slider slot for DIC U-P5REMC Motorized centerable quintuple revolving nosepiece with slider slot for DIC 146 32 7 ° 105 108 332 (depth) 310 125 212 216 * Extension cord U-RMT (1700mm)[...]

  • Page 41

    MOTORIZED UNITS 40 Unit: mm ø 78 R88 147.5 108 135 170.5 148 312.5 9 56 58.2 58.4 14.8 62.5 71 * Consult your Olympus dealer about the motorized focus. Cable length: 2000mm Weight: 3.3kg U-AFA1M Active auto focus unit Weight: 1.0kg U-FWR Motorized reflected filter wheel Accomplish maximum 6 filter position exchange 2000 147.9 180.5 130 58.5 24.5 4[...]

  • Page 42

    MOTORIZED UNITS 41 51 77 206 310.5 77 133 341.6 334 56 56 169 92 54 70 4 75.5 82.3 50 91.5 210 70 100 214 104 33.6 3 BXFMA-F Motorized illumination with power focus A motorized microscope unit for integration with your equipment. Motorized operations such as revolving nosepiece up/down, objective lens switching, aperture diaphragm open/close, and b[...]

  • Page 43

    DEEP UL TRA VIOLET OBSERV A TION SYSTEM 42 Deep ultraviolet observation system This module adds a deep ultraviolet (248nm) optical system to a general microscope. An ultra-high resolution observation is executed by using an extremely short wavelength ray. Unit: mm 108 258.5 69 79 ø 8 (light guide) 34 30.4 105.8 2000 +200 0 or 5000 +200 0 (39.5) 6 [...]

  • Page 44

    1. Field Number (F.N.) and Practical Field of View The field number (F.N.) is referred to as the diaphragm size of eyepiece in mm unit which defines the image area of specimen. The diaphragm diameter actually seen through eyepiece is known as the practical field of view (F.O.V.) which is determined by the formula: F.O.V. = Eyepiece F.N. (mm) Object[...]

  • Page 45

    • Total magnification on the video monitor: m (ob) =50 × , M (video camera adapter) is 0.5 × from Figure 1 and monitor magnification is 46.2 × from Figure 3. M (monitor observation) = M (ob) × M ( video camera adapter ) × monitor magnification =50 × 0.5 × 46.2=1155 × • Practical filed of view for video observation(horizontal side): M (o[...]

  • Page 46

    9. Aberrations A difference between an ideal image and an actual image that passes through an optical system is called an “ aberration. ” 9.1 Requirements for Ideal Image Formation The following three requirements must be satisfied to form an image with no aberration, or an ideal image. (i) All the light rays coming from a single point and pass[...]

  • Page 47

    (4) Field curvature An image plane of an object on a plane perpendicular to an optical axis does not always become a plane perpendicular to the optical axis, but it generally becomes a curved plane. This symptom is called “ field curvature. ” When field curvature is present, the image is more displaced as it becomes closer to the periphery of t[...]

  • Page 48

    Specifications are subject to change without any obligation on the part of the manufacturer. Printed in Japan M1606E-1106B • OLYMPUS CORPORATION has obtained ISO9001/14001.[...]