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Agilent Technologies G1978B manual

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  • Página 1

    Agilent T echnologies Agilent G1978B Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide[...]

  • Página 2

    2 Multimode Source for 6100 Series Singl e Quad LC/MS Set-U p Guide Notices © Agilent T echnologies, Inc. 2008 N o p a rt o f t h i s m a n u a l m a y be r e p r o d u c e d i n any form or by any means (including elec- tronic storage and retrieval or translation into a foreign language) without prior agree- ment and written consen t from Agilent[...]

  • Página 3

    Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 3 In This Guide This guide explains how to install, maint ain and troubleshoot your nanoelectospray ion sour ce. 1 Installation Thi s c ha pte r tel ls y ou ho w to i ns tall the mul ti mod e i on so ur ce . 2 Ve r i f i c a t i o n Thi s c ha pte r tel ls y ou ho w to i ns tall the mu[...]

  • Página 4

    4 Multimode Source for 6100 Series Singl e Quad LC/MS Set-U p Guide[...]

  • Página 5

    Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 5 Content 1 Installation 7 Installation 8 Step 1. Prepare to install 8 Step 2. Install the HV control PCA and cables 9 Changing Sources 13 T o remove the m ultimode source 13 T o convert from multimode to ESI, APCI or APPI 14 T o convert from ESI, APCI or APPI to the multimode source 1[...]

  • Página 6

    6 Multimode Source for 6100 Series Singl e Quad LC/MS Set-U p Guide Contents[...]

  • Página 7

    7 Agilent G1978B Multimode Source fo r 6100 Series Single Quad LC/MS Set-Up Guide Agilent T echnologies 1 Installation Installation 8 Step 1. Prepare to install 8 Step 2. Install the HV control PCA and cabl es 9 Changing Sources 13 T o remove the multimode source 13 T o convert from multimode to ESI, APCI or APPI 14 T o convert from ESI, APCI or AP[...]

  • Página 8

    8 Multimode Source for 6100 Series Singl e Quad LC/MS Set-U p Guide 1 Installation Installation Installation This section explains ho w t o insta ll t he G1978B multimode source on a 6100 Series Single Quad LC/MS instrument. The G1978A multimode source is not suppor ted on these instr uments. The 6100 Series Single Quad LC/MS wit h multimode source[...]

  • Página 9

    Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 9 Installation 1 Step 2. Install the HV control PCA and cables • V alv e BD-APCI supply , p/nG1960-60802 • V alv e BD-APCI Needle Interlock, p/n G1960-60856 Figure 2 From left to right: G1946-80058, G1960-60802 and G1960-60856 Step 2. Install the HV control PCA and cables 1 Tur n o[...]

  • Página 10

    10 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 1 Installation Step 2. Install the HV control PCA and cables Figure 4 Connecting th e valve PCA to the multimode HV p ower supply . 6 Install t he APCI HV po wer suppl y. The APCI HV po wer supply is located at the e nd of the A UX Mo du le. 7 Connect ribbon cable betw een va lv e P[...]

  • Página 11

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 11 Installation 1 Step 2. Install the HV control PCA and cables Figure 6 Connecting the V cap and V chamber cables to the powe r supply . 9 Connect the long ribbon cable, p/n G1960-60802, from the APCI HV po w er sup ply to the va lv e PC A. Figure 7 Connecting the A PCI HV power sup[...]

  • Página 12

    12 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 1 Installation Step 2. Install the HV control PCA and cables Figure 9 Connecting the HV PCA to the chassis. 12 Close t he A UX Module cover and reconnect all cables. 13 Inst all the multimo de source to t he system and c onnect all connectors. Figure 10 Installing th e multimode sou[...]

  • Página 13

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 13 Installation 1 Changing Sources Changing Sources T o remove the multimode source Do the follo wing ste ps to remov e the m ultimode source. 1 Set the source temperatures f or vaporizer heater and dr ying gas heater t o minimum values to cool the source. Use the T une > Instr um[...]

  • Página 14

    14 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 1 Installation To convert from multimode to ESI, APCI or APPI 10 Place the source sh ipping co ver on the source. T o convert from multimode to ESI, APCI or APPI 1 If the s ource to be inst alled is an APPI source, disconnect the multimode high vo ltage PCA RS-232 serial cable fr om[...]

  • Página 15

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 15 Installation 1 To convert from ES I, APCI or APPI to the multimode source T o convert from ESI, APCI or APPI to the multimode source 1 Swi tch to the MSD T une view . 2 Select Instr ument/Set Spray Chamber and set all gas f l o ws and tem pera tur es to 0 . • Dr ying Gas (L/min)[...]

  • Página 16

    16 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 1 Installation To convert from ESI, APCI or APPI to the multimode source Figure 11 Standard spray shiel d and capillary cap for ESI or A PCI 10 Remov e the capillar y cap. If need ed, moisten a clean clot h with isopropyl alcohol and wipe the capillar y cap. See Figur e 1 2 . Figure[...]

  • Página 17

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 17 Installation 1 To convert from ES I, APCI or APPI to the multimode source Figure 13 Multimode spray shield 13 Screw the multimo de spray shield into the holder for the spra y shield. See Fig ure 1 4 . Figure 14 Multimode spray shie ld installed 14 Remov e the shippi ng co ver from[...]

  • Página 18

    18 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 1 Installation To convert from ESI, APCI or APPI to the multimode source Figure 15 Multimode Spray Cha mber 15 Inst all the spray chamber on the spray chamber mount. Figure 16 Multimode source with I-Button 16 Install t he nebulizer on t he mu ltimode source spra y chamber . I-Butto[...]

  • Página 19

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 19 Installation 1 To convert from ES I, APCI or APPI to the multimode source Figure 17 No nebulizer on top of the multimode source 17 Connect the 1/8-inch nebulizer gas tu bing from the LC/MS mainframe to the nebulizer gas f itting. See Fi gu re 18 . Figure 18 Nebulizer with ga s tub[...]

  • Página 20

    20 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 1 Installation To convert from ESI, APCI or APPI to the multimode source 18 Connect the LC/MS sample tubing to the LC/MS divert er valv e inlet filter . See Figu re 19 on page 20. Figure 19 LC/MS sample tub ing connected to LC/MS in let filter 19 If you are installin g the multimod [...]

  • Página 21

    21 Agilent G1978B Multimode Source fo r 6100 Series Single Quad LC/MS Set-Up Guide Agilent T echnologies 2 V erification T o verify sensitivity for 6120 Qu ad - G1978B Multimode, Positiv e SIM Modes 22 T o verify sensitivity for 6130 and 614 0 Quads - G1978B Multimode, Positive SIM Modes 24 T o verify sensitivity for 6130 and 614 0 Quads - G1978B M[...]

  • Página 22

    22 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 2 Verification To verify sensitivity for 6120 Quad - G1978B Multimode, Positive SIM Modes T o verify sensitivity for 6120 Quad - G1978B Multimode, Positive SIM Modes Preparation 1 Transfer 1 mL of 5 ng/µL reserpine (A gilent G2423A) to a 50 mL v olumetric f lask. Use a clean g radu[...]

  • Página 23

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 23 Verification 2 To verify sensitivity for 6120 Quad - G1978B Multimode, Positive SIM Modes Sensitivity V erification for Mult imode in ESI Positive SIM Mode 1 Star t up t he ChemSt ation software. 2 Change the view to MSD T une , and star t an Autotune. After the auto tu ne com ple[...]

  • Página 24

    24 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 2 Verification To verify sensitivity for 613 0 and 6140 Quads - G1978B Mu ltimode, Positive SIM Modes When the meth od is finished, a repor t prints showing the signal-t o-noise ratio f or the five blank and five sample peaks, and a blank-subtracted average of the sample peaks. The [...]

  • Página 25

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 25 Verification 2 To verify sensitivity for 6130 and 6140 Quad s - G1978B Multimode, Positive SIM Modes Sensitivity V erification for Multim ode in ESI, Positive SIM Mode 1 Star t up t he ChemSt ation software. 2 Change the view to MSD T une , and star t an Autotune. After the auto t[...]

  • Página 26

    26 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 2 Verification To verify sensitivity for 613 0 and 6140 Quads - G1978B Mu ltimode, Positive SIM Modes experiments t o achieve maxi mum instrument sensitivity 7 Review the results. When the meth od is finished, a repor t prints showing the signal-t o-noise ratio f or the five blank a[...]

  • Página 27

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 27 Verification 2 To verify sensitivity for 6130 and 6140 Quad s - G1978B Multimode, Positive Scan Modes T o verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive Scan Modes Preparation 1 Transfer 1 mL of 5 ng/µL reserpine (A gilent G2423A) to a 50 mL v olumetric f[...]

  • Página 28

    28 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 2 Verification To verify sensitivity for 613 0 and 6140 Quads - G1978B Mu ltimode, Positive Scan Modes Sensitivity V erification for Multim ode in ESI, Posi tive Scan Mode 1 Star t the ChemSt ation software. 2 Change the view to MSD T une , and star t an Autotune. After the auto tu [...]

  • Página 29

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 29 Verification 2 To verify sensitivity for 6130 and 6140 Quad s - G1978B Multimode, Positive Scan Modes When the me thod is f inished, a repor t prints sho wing t he signal-to-noise ratio f or the f ive blank and five sam ple peaks, and a blank-subtracted av erage of t he sample pea[...]

  • Página 30

    30 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 2 Verification To verify sensitivity for the G1 978B Multimode using Multiple FIA method in Mixed Mode operation T o verify sensitivity for the G1978B Multimode using Multiple FIA method in Mixed Mode operation Preparation 1 Do an autotune if necessar y. After the auto tu ne has co [...]

  • Página 31

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 31 Verification 2 To verify sensitivity for the G1 978B Multimode using Multiple FIA method in Mixed Mode operation d Click OK . 8 Repeat f or the MMCheckSL _CI.M data f ile. Use the same subdirector y and change Dat a File to Multi_CI . 9 Repeat f or the MMCheckSL _MX.M data f ile. [...]

  • Página 32

    32 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 2 Verification To do an autotune T o do an autotune Tuning t he multimode source is done in MM-APCI mode only. A utotune is done from the same menu as with all sources. • Fr om the MSD Tune view , select Instrument > Autotune menu it em. The tune report will have a header with [...]

  • Página 33

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 33 Verification 2 Example of multimode verification report Example of multimode verification report[...]

  • Página 34

    34 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 2 Verification Example of multimode verification report[...]

  • Página 35

    35 Agilent G1978B Multimode Source fo r 6100 Series Single Quad LC/MS Set-Up Guide Agilent T echnologies 3 Methods T o setup a method to use the multimode source 36 T o create a method for positive/negative mixed mode operation 37 T o create a method for altern ating ESI and APCI operation 39 Th i s c hap te r d e sc ri bes the ta sks that yo u n e[...]

  • Página 36

    36 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 3M e t h o d s To setup a method to use the multimode source T o setup a method to use the multimode source T o ha ve y our method use a multimode source, fo llo w these steps: 1 Open the MSD Spray Chamber dialog bo x by clicking Instrument > MSD Spray Chamber in the Met hod and [...]

  • Página 37

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 37 Methods 3 To create a method for posit ive/negative mixed mode opera tion T o create a method for positive/negative mixed mode operation 1 Open the MSD Spray Chamber dialog bo x by clicking the Instr ument > Set Up M SD S ig na ls in the Met hod and Run Control vie w . 2 Select[...]

  • Página 38

    38 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 3M e t h o d s To create a method for posit ive/negative mixed mode op eration Figure 21 Positive/Negativ e polarity switching using Set Up MSD Signals dialog box Polarity options for S ignal 1 and Signal 2[...]

  • Página 39

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 39 Methods 3 To create a method for alternating ESI and APCI operation T o create a method for alternating ESI and APCI operation 1 Open The MSD Spray Chamber dialog bo x by clicking Instrument > MSD Spray Chamber in the Method and Run Control view . 2 Set Met hod Spray Chamber to[...]

  • Página 40

    40 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 3M e t h o d s To create a method for altern ating ESI and APCI operation 9 Click the OK button. Figure 23 MM-ES and MM- APCI switching using Set Up MSD Si gnals dialog box NOTE In general, use the mi xed mode operation (MM-ES+ APCI setting with Si gnal 1) instead of alternating MM-[...]

  • Página 41

    Multimode Source for 6100 Series S i ngle Quad LC/MS Set-Up Guide 41 Index Index A autotune , 32 C converting from ESI, APCI or AP PI , 15 converting to E SI or APCI , 14 D diverter valv e inlet filter , 20 E ESI convert from , 15 convert to , 14 I installation , 7 , 21 L LC/MSD sample tubing , 20 M method alternating ESI and APCI , 39 basic setup [...]

  • Página 42

    42 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide Index[...]

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    © Agilent T echnologi es, Inc. 2008 Printed in USA First Editio n, December 20 08 *G1978-90070* G1978-90070 www .agilent.com Agilent T echnologies In This Book This book contains installation, operation, maintenance and troubleshooting instruction fo r the Multimode Source fo r 6100 Series Single Quad LC/MS.[...]