Leica M125 B manual

Living up to Life Leica M 1 25 B Leica M 1 65 C B User Manual[...]

Leica M series User Manual 2 All rights to this documentation ar e held by Leica Microsystems CMS GmbH. Repr oduction of text or illustrations (in whole or in part) by print, photocop y , microfilm or other method (including electronic systems) is not allowed without express written permission fr om Leica Microsystems CMS GmbH. The instructions co[...]

Leica M series User Manual 3 F unc tion of the Microsc opes The Leica M125 B and L eica M165 C B micro - scopes, f or which this User Manual has been written, are designed f or routine examinations of cell and tissue cultures , liquids, and sedi - ments. This includes examining specimens taken from the human body for the purpose of gaining in[...]

Leica M series User Manual 4 General Notes Use in clean rooms The Leica M series can be used in clean r ooms without any problems . Cleaning ★ Do not use any unsuitable cleaning agents, chemicals or techniques for cleaning . ★ Never use chemicals to clean color ed sur faces or ac cessories with rubberized parts. T his could damage the surfaces,[...]

Leica M series User Manual 5 Impor tant Safety Notes User Manual This User Manual describes the special func - tions of the individual modules of the Leica M stereomicr oscopy series and contains impor - tant instructions for their operational safety , maintenance , and accessories. Y ou can combine individual system articles with articles from ext[...]

Leica M series User Manual 6 Symbols U sed W arning! Safety hazard! • This symbol indicates especially impor - tant information that is manda tory to read and observe. F ailure to comply can cause the following: ★ Hazards to personnel ★ F unctional disturbances or damaged instruments W arning of hazardous electrical voltage  This symbol[...]

Leica M series User Manual 7 Safety Instruc tions Description ★ The individual modules fulfill the highest requirements f or obser va tion and docu - mentation of Leica stereomicroscopes of the M series. Intended use of instrument ★ Leica Microsystems microscopes are opti - cal instruments for impro ving the visibility of objects or specimens [...]

Leica M series User Manual 8 Safety Instruc tions (con tinued) Responsibilities of person in charge of instrument ★ These Safety Instructions must be available at the workplace. Ensure that: ★ The M series stereomicr oscopes and accessories are oper ated, maintained and repaired b y authorized and trained person - nel only . ★ All operators h[...]

Leica M series User Manual 9 Safety Instruc tions (con tinued) Legal regulations ★ Observe the generally applicable statu - tory and countr y-specific r egulations for accident prev ention and environmental protection. EC Declaration of C onformity ★ Electrically operated accessories are constructed based on the state of the art of technology [...]

Leica M series User Manual 10 Safety Instruc tions (con tinued) Light sources: saf ety regulations ★ Light sources pose a pot ential risk from glare. Therefor e, lamps have t o be operated in closed housings and in installed condi - tion. ★ Never look directly into the beam path (blinding hazard). ★ Do not select a white, strongly r eflectiv[...]

Leica M series User Manual 11 Con tents Quick Start Guide The F astest Route to Success 29 Overview of an M S eries Microscope 30 The correct interpupillary distance 31 Using the Eyepiec es 32 F ocusing 33 Adjusting the Resistance of the F ocus Drive 34 Changing the Magnification (Zoom) 35 Click Stops and Magnifications 36 Parfocality: More Comfo[...]

Leica M series User Manual 12 Optical Acc essories The Differ ent T ypes of Objectives 46 Bases Leica TL5000 Ergo T ransmitted Light Base: C ontrols 48 Leica TL5000 Ergo T ransmitted Light Base: Opera tion 49 T echnical Data Leica TL5000 Ergo T ransmitted Light Base 52 Appendix Calculating the T otal Magnification and Field of View Diameter 54 Ca[...]

Leica M series User Manual 13 T he Leica M S eries[...]

Leica M series User Manual 14 Congr atulations! Congra tulations on obtaining your new Leica M series ster eomicroscope. W e are convinc ed it will exceed y our expectations, as never befor e have we applied our decades of experience in the ar eas of optics, mechanical engineering and ergonomics in such an uncompr omising manner . The Leica M serie[...]

Leica M series User Manual 15 A Step T owards Infinity Ever since their in troduction by Horatio S. Gree - nough, stereomicr oscopes have work ed accord - ing to the optical principles based primarily on Ernst Abbe's resear ch. For ov er a century, ingenious optics designers and engineers hav e worked to push magnifica tion, resolution and i[...]

Leica M series User Manual 16 The Electronics: Comf or t, C onv enience and Safety for y our Experiments Never befor e have electronics been used as extensively in a Leica series as in the new M series lineup . Optics carrier , tripod, base and illuminator are all c onnected using elec trical contacts—which provides a number of advan - tages. Fe [...]

Leica M series User Manual 17 Leica engineers w ere careful to ensur e that the new Leica M series—like its pr edecessors— remains compatible with existing series . This means that objectives, bases, tubes and so on can be reused. Objectives All new objectives of the Leica M series are par f ocal. If you prefer , you can con tinue to use the pr[...]

Leica M series User Manual 18 On W e Go If your new Leica micr oscope has already been assembled and commissioned by your Leica consultant , click here to skip through the installation instructions and go directly to the Quick Start Guide on page 28 . If, on the other hand , you are assembling the microscope y ourself, con tinue with the " Ass[...]

Leica M series User Manual 19 Assembly[...]

Leica M series User Manual 20 The first step is to c onnect the focusing column of the M series to the corresponding base . T ools used ★ Allen key , 3 mm Assembling the column adapter 1. Securely install the column adapter on the column using the four included screw s. Assembling the focusing drive 2. Securely screw the focusing c olumn to the [...]

Leica M series User Manual 21 T ools used ★ Allen key , 4 mm Assembling the Optics Carrier 1. Place the optics carrier on the focus - ing column so that the screw fits int o the thread pro vided and the lug fits into the groove. 2. Press the optics carrier backwar ds to the focusing column and screw it in plac e using your other hand. Optics ca[...]

Leica M series User Manual 22 T ube All intermediate tubes tha t fit between the optics carrier and the binocular tube are fitted in the same manner . T ools used ★ No tools requir ed. Prepara tions 1. Unscrew the positioning screw and r emove the protective co ver . Assembling the tube 2. P ush the tube (for example, the inclined binocular tub[...]

Leica M series User Manual 23 Ey epieces T ools used ★ No tools requir ed. Magnification range Y ou can extend the overall magnifica tion range using available 10×, 16×, 25× and 40× wide- field eyepieces for persons w earing glasses. Prepara tion 1. If you want t o use an optional graticule, insert it now ( page 44). 2. Remove the plastic [...]

Leica M series User Manual 24 Objective T ools used ★ No tools requir ed. Prepara tion 1. Remove the protective cap on the optics carrier by turning it. Attaching the objective • Hold the objective firmly during assem - bly and disassembly so that it does not fall onto the stage plate . This applies particu - larly to the 2x planapochromati[...]

Leica M series User Manual 25 TL5000 Ergo transmitt ed light base Unpacking the base The base is deliver ed with the adapter plate installed. Make sure the instruments ar e unpacked on a flat, sufficiently dimensioned , and non-slip sur face .  Refer also to the separa tely provided User Manual for the TL5000 Ergo trans - mitted light base. Foc[...]

Leica M series User Manual 26 The new Leica M series features extensive encoding with which various microsc ope data and settings can be read out and repr oduced later . ★ The interface to the optics carrier is on the column. The terminals The connection to other instruments is made using the terminals on the rear side of the column: 1. 2×CTL2 t[...]

Leica M series User Manual 27 The integr ated cable duct in the column enables a neat cable layout ar ound the micro - scope. F or example, the USB or FireW ire cables of the camera can be stow ed in the cable duct. Feeding the cables 1. Unscrew the three scr ews on the cable duct. 2. Remove the c over of the cable duct. 3. Place the cables in the [...]

Leica M series User Manual 28 Q uick Star t Guide[...]

Leica M series User Manual 29 The F astest Route to Suc cess Y our Leica ster eomicroscope has been deliver ed in completely assembled condition by your L eica partner, and na turally you want to get righ t to work. Therefor e, your next step should be to study the Quick Start Guide, which outlines the most important steps at a glance. This manual [...]

Leica M series User Manual 30 Overview of an M Series M icr oscope 1 Switch click stops on/off 2 Magnification changer (zoom) 3 Coarse/fine focusing 4 Transmitted light base 5 Interchangeable objective 6 Built-in iris diaphragm 7 Fastening screw for the binocular tube (or accessories) 8 Adjustable eyepiece tubes 9 Fastening screws for the eyepie[...]

Leica M series User Manual 31 The corr ec t int erpupillar y distanc e The interpupillary distance is correctly set if you see a single circular image field when looking at a specimen. If you are still a novic e microscope user , you may need a short time to become accustomed to this. Not to w orr y—after a little while , it will become automati[...]

Leica M series User Manual 32 If you wear glasses: 1. Hold the eyepiece firmly and r otate the eyecups count erclockwise towards the r ear , as otherwise the viewing distance is too great. 2. I f an ey epiece is equipped with the inte - grated dioptric c orrection, turn the value to the "0" mark. By the way , one benefit of viewing with[...]

Leica M series User Manual 33 F ocusing F ocusing raises or lowers the ster eomicroscope using the focusing drive . The specimen detail is brought into sharp f ocus as soon as it is in the focal point of the objective. ★ The focusing driv e can be operated either left- or right-handed. Focusing ★ The inner , coarse adjustment is used for coveri[...]

Leica M series User Manual 34 Adjusting the Resistanc e of the F ocus Drive Adjusting the resistance Is the focusing drive t oo loose or too tight? Does the equipment tend to slide down wards? The resistance can be adjust ed individually depending on the equipment weight and personal prefer ences as follows: 1. Grip the outer drive knobs with both [...]

Leica M series User Manual 35 Changing the Magnification (Zoom) All M series microscopes hav e an integrat ed zoom. The name indicates the zoom range cover ed: ★ Leica M125 B = 12.5:1 ★ Leica M165 C B = 16.5:1 The rotary knob for the zoom can be used either left or right-handed. Zooming 1. Look int o the eyepieces. 2. F ocus on the specimen. 3[...]

Leica M series User Manual 36 The zoom button can optionally be oper ated either with or without ratchet steps . Continu - ous zoom is possible when the click stops are disabled, which many users find c onvenient. On the other hand, when the click stops ar e enabled, photogr aphs, measurement results etc. can be repr oduced more accura tely . Enab[...]

Leica M series User Manual 37 Parfocality: More Comf or t and C onv enience for Y our W ork All Leica ster eomicroscopes are parfocally matched, meaning tha t you can view a focused specimen from the low est to the highest magnification without having to r efocus. The focus needs to be r eadjusted only if you want to view a specimen detail that is[...]

Leica M series User Manual 38 The iris diaphragm in the optics carrier of y our M series microscope has the same purpose as those in a camera shutter: it regulat es the avail - able light, which changes the depth of field. The "depth of field" (or " depth of focus") is the area of a specimen that is brought int o sharp focus. [...]

Leica M series User Manual 39 E y epiec es[...]

Leica M series User Manual 40 Magnification F ac tor of the E yepiec es An eyepiece not only makes it possible t o look passively into the micro - scope, but also has a critical eff ect on the maximum magnification. T he magnification factor is between 10x and 40x. The following e yepieces are a vailable for the M series: Magnification Dioptri[...]

Leica M series User Manual 41 Health Notes Pot ential sources of infection • Direct contact with eyepieces is a potential transmission method f or bacterial and viral infections of the eye. The risk can be kept to a minimum by using individual eyepieces or detachable ey ecups. Eyecups can be ordered separa tely . Please contact your Leica part[...]

Leica M series User Manual 42 All Leica eyepiec es are also available with built- in dioptric correction, allowing the microscope to be used without glasses even by those with vision problems. The correction comprises ±5 diopter settings. Using the Dioptric Corr ec tion 1. Set the dioptric corr ection of both eyepieces to the mid position ("0[...]

Leica M series User Manual 43 Adjusting 1. Set the dioptric correction for both eyepieces to "0" . 2. Select the lowest mag nification and focus on a flat specimen. 3. Selec t the highest magnification and r ead - just the sharpness. 4. Selec t the lo west magnification again, but do not look into the eyepiec es. 6. Rotate the ey epie[...]

Leica M series User Manual 44 Graticules 2. Clamp the g raticule on the insert, applying moderate pr essure. Ensure tha t the grati - cule fits tightly . 3. Screw the insert and graticule firmly into place and replace the ey epiece in the tube. 4. Y ou can now align the g raticule by rotating the eyepiece in the tube and then tight en - ing it us[...]

Leica M series User Manual 45 Objec tiv es and Optic al A cc essories[...]

Leica M series User Manual 46 The Differ ent T ypes of Objec tives T o meet the various requiremen ts regarding imaging properties, there is a choice of high- quality interchangeable planachroma tic and planapochromatic objectives and also low er- priced inter changeable achromatic objectives. ★ Achroma tic objectives are par ticularly suited fo[...]

Leica M series User Manual 47 Bases[...]

Leica M series User Manual 48 Leica TL5000 Ergo T ransmitted Ligh t Base: Contr ols  Refer also to the separa tely provided User Manual for the TL5000 Ergo trans - mitted light base. 1 Pow er switch for the base 2 Pow er supply connection 1 On/Off switch for the light source / Press and hold for 5 seconds to r eset the base to factory default s[...]

Leica M series User Manual 49 2. Press the on/off swit ch on the right side once to turn on the illumination. Switching the transmitt ed light base on and off 1. Switch on the transmitted ligh t base with the power switch on the r ear side. Leica TL5000 Ergo T ransmitted Ligh t Base: Operation  The transmitted light base must only be connected[...]

Leica M series User Manual 50 4 Press the on/off switch on the right side once again to turn off the illumination on the base. Leica TL5000 Ergo T ransmitted Ligh t Base: Operation ( Con tinued) 3. Look through the eyepiece and adjust the light intensity using the control on the right side. The intensity of the illumination is visualized by the L[...]

Leica M series User Manual 51 T echnic al Da ta[...]

Leica M series User Manual 52 Leica TL5000 Ergo T ransmitted Ligh t Base Light source Light source LED Illuminated field: Bright field ∅ 65 mm Illuminated field: Dark field ∅ 40 mm Relief Contrast S ystem (RC™) Ye s Internal shutter/lamp contr ol Ye s Integrated filt er holder Ye s Matching of high num. aperture Ye s Remote contr ol opti[...]

Leica M series User Manual 53 Appendix[...]

Leica M series User Manual 54 Calculating the T otal Magnification and Field of Vie w Diameter Paramet er MO Magnification of objective ME Magnification of eyepiece z Magnification changer position q T ube fac tor , e.g . 1.5× for coaxial incident light, 1.6× f or 45° Ergo T ube™ r F ac tor 1.25× if the planachroma tic and planapochromati[...]

Leica M series User Manual 55 Care , Maintenance, C ontac t P ersons W e hope you enjoy using your high-perfor - mance microscope . Leica microscopes ar e renowned f or their robustness and long service life. Observing the following care and clean - ing tips will ensure that ev en after years and decades, your L eica microscope will con tinue to wo[...]

Leica M series User Manual 56 Care , Maintenance, C ontac t P ersons (continued) Protection from dirt Dust and dir t will aff ect the qualit y of your results. ★ Put a dust cov er over the microsc ope when it will not be used for a long time. ★ Use dust caps to prot ect tube openings, tubes without eyepieces , and eyepieces. ★ Keep accessori[...]

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