Princeton 4300 manual

i 4411 -0100 Version 2.E September 15, 2011 *4411-0 100*[...]

ii  Copyright 200 3-2011 Princeton I nstruments, a div ision of Roper Scien tific, Inc. 3660 Quakerbridg e Rd Trenton, NJ 08619 TEL: 800-874- 9789 / 609- 587-9797 FAX: 609-587- 1970 All rights reserv ed. No part of this publication m ay be reproduced by any m eans without the written permission of Pri nceton Instrum ents, a division of Roper Sci[...]

iii Table of Contents Chapter 1 Introduction ......................................................................................... 9 When Flexibility Counts ..................................................................................................... 9 Fiberoptic Experti se ...............................................................[...]

iv PI - SCX System Manual Version 2.E iv Verifying C ontroller Voltag e Setting ................................................................................ 28 Mounting the Cam era ................................................................................................ ....... 29 Installing the App lication Softwa re ...................[...]

Table of Conten ts v v TTL In ........................................................................................................................ 61 Buffered vs. Latch ed Inputs ....................................................................................... 62 TTL Out ....................................................................[...]

vi PI -SC X System Manual Version 2.E vi Controller TTL I /O ................................................................................................ ........... 88 Controller I /O .................................................................................................................... 88 Controller I nterface ...................[...]

Table of Conten ts vii vii Figure 15. PI- SCX:4300 and PI -SCX:4096 Cam era Back Panel ................................... 39 Figure 16. Block Diagram of Lig ht Path in Sy stem ......................................................... 41 Figure 17. Exam ple of WinView Dat a Acquired from First Light Procedu re ................. 45 Figure 18. Array [...]

viii PI - SCX System Manual Version 2.E viii Tables Table 1. PCI Driver Files and Loca tions ................................ ......................................... 32 Table 2. USB Driv er Files and Loc ations ........................................................................ 34 Table 3. Cam era Timing Modes ...............................[...]

9 Chapter 1 Introduction When Flexibility Coun ts Princeton I nstruments knows tha t a camera desig ned to detect x- ray photons for sci entific research needs to be flexible. X-ray scattering from complex cry stals such as proteins requires hig h dynamic range and low-noise readout, w hereas scattering f rom m aterials like polym ers, fibers, and [...]

10 PI - SCX System Manual Version 2.E 10 Low -Noise Configurations The PI- SCX system provides eith er a forced-air or wat er-cooling option to reduce dark current. The the rmoelectric (Peltier) design and p recision elect ronics provide v ery hig h precision as well a s the cap ability to set the requ ired tem perature. For higher perfo rmance, mo[...]

Chapter 1 Introduction 11 11  ST -1 33 C on tr ol ler : C on tr ols th e c am e ra an d ex pe ri m en t, i nc lud ing t he te m p er atu re set po in t. C ol lec ts t h e dat a a n d t ra nsf ers it to th e co m put e r for fu rth er pro ce ss i ng .  Camera-Control ler Cable: This 10' cable ( 6 0 50 -0 4 8 4) connects from the DETECTOR [...]

12 PI - SCX System Manual Version 2.E 12 About this Manual Ma nual Organization This manual prov ides the user w ith all the info rmation needed to install a PI-SCX camera and place it in oper ation. Topics cov ered include a d etailed descrip tion of the cam era, installation, c leaning, specific ations and more. Notes: 1. The general iden tifier [...]

Chapter 1 Introduction 13 13 Safety Related Symbols Used in this Manual C a ut ion ! T h e use o f thi s s y m bo l on eq ui pm e n t ind ica tes t hat o ne o r m o re n ea rby it e m s s h o ul d n o t be o pe ra ted w it h o ut f ir s t con su lt i ng t he ma nu al . Th e sa m e sy mb o l app e a rs in t he ma nua l a dja c en t t o th e te x t t[...]

14 PI - SCX System Manual Version 2.E 14 Precautions To prevent perm anently dam a ging the system, pleas e observe the following preca utions:  Always switch of f and unpl ug the ST-133 Controlle r before chang ing your system configuration in any way.  The CCD array is very sensitiv e to static elec tricity. Touching th e CCD can dest roy i[...]

15 Chapter 2 System Component Descriptions PI -SCX Camera CCD Array: PI - SCX currently offers thre e CCD form ats: the 1340x1300 array with 20x20 µm pixels, the 2084x2084 a rray with 24x24 µ m pixels, and the 4096x409 6 array with 15x15 µm pixels. All are sci entific grade full-fram e a rrays with MPP. The application of ind ium tin oxide (I TO[...]

16 PI - SCX System Manual Version 2.E 16 connected to the P eltier. CCD temperature is cont rolled and m onitored by the host computer and the ST - 133 Control ler. Electronics: The cam er a electronics encl osure contains th e preamplifier and array driver board. This design keeps all signal leads to the pream plifier as short as p ossible and als[...]

Chapter 2 System C omponent Descriptions 17 17 ST - 133 Controller Electronics: The M odel ST - 133 is a compact, hig h performance CCD Camera Controller for ope ration with Prin ceton Instrum ents cam eras. Designed for hig h speed and high perform ance image acquis ition, the ST - 1 33 offers data transf er at speeds up to 5 megapixel per second,[...]

18 PI - SCX System Manual Version 2.E 18 Always turn the pow er off at t he Controller befo re connecting or disconnecti ng any cable that interconnec ts the cam era and controller o r serious dam age to the CCD m ay result. This dam age is NOT covered by the manufactu rer’s warranty. Figure 3. ST-133 R ear Panel WARNING![...]

Chapter 2 System C omponent Descriptions 19 19 Rear Panel Features : T h e de sc rip tio n s of t he rea r pa n el c on ne ct ors ar e k ey ed to Figure 3 . D ep en di ng o n y our sy st em , e ith e r the TA XI or th e U SB 2 .0 I nt erf a c e Con tro l M o d u le w ill b e ins tal l ed in th e se con d fr om the le ft slo t ( as y ou fa ce t h e [...]

20 PI - SCX System Manual Version 2.E 20 # Feature 12. Shutter Sett ing Selector: Sets the shutter hold volta ge. Con tact factory for the cor rect shutter setting for a re mote x-r ay shutter. 1 3. R emo t e Sh u t te r C on ne c to r : Prov i de s sh utt er- d riv e an d h ol d vo l t ag es f or a P ri n ce t on In s t rum ent s 25 mm ex t er na [...]

Chapter 2 System C omponent Descriptions 21 21 T A X I: Th e s tan dar d 2 5' ( 7.6 m) c ab le (6 05 0- 0148 - CE ) h as D B- 9 M ale co nn ec tor s w ith s cr ew- d o wn l ock ing h a rd w ar e. The TA XI ( Se ri a l co m m u n ic ati o n ) cab le int erc o nn ect s t he Se ri al Com c on nec t or o n the r ea r o f t he ST -1 33 w ith th e P[...]

22 PI - SCX System Manual Version 2.E 22 User Manuals PI -SCX System User Manual: This m anual descri bes how to instal l and use the PI-SC X system components. WinView/32 User Manual: This m anual descr ibes how to inst all and use the application prog ram. A PDF v ersion of this m anual is provided o n t he instal lation CD. Additional in formati[...]

23 Chapter 3 Installation Overview The list and diagrams below briefly describe the sequence of actions required to hookup your system and prepare to gather data. Refer t o t he indic ated references for more detailed information. T his list assumes that the application software is Princeton I nstruments WinView/32. Action Reference 1. If the syste[...]

24 PI - SCX System Manual Version 2.E 24 Action Reference 11. Turn on the com puter and beg in running WinView/3 2. When the computer boots, you m ay be a sked for the location of the in terface drivers. Chapter 4 System Setup, page 31 (PCI drivers) or page 32 (USB driv ers) WinView/32 m anual 12. Enter the hardwar e setup inform ation or load th e[...]

25 Chapter 4 System Setup Unpacking the System During the unpa cking, check the system com ponents for possible signs of shippin g damag e. If there are any, notify Princ eton Instrum ents and file a cla im with the ca rrier. If damag e is not apparent but cam era or controller sp ecifications cannot be achiev ed, internal dam age may hav e occurre[...]

26 PI - SCX System Manual Version 2.E 26 System Requirements Environmental Operating tem perature: 0ºC to +30 ºC; Operating tem perature range ov er which system specifications can b e met: +18ºC to +23ºC Relative hum idity <50% nonconden sing. Ventilation Camera: Allow at least one inch of clearance fo r side and rear air vents. ST - 133: T[...]

Chapter 4 System Setup 27 27 PI -SCX :4096 and PI-SCX :4300 Two barbed 3/8  O.D . coolant port s are located at the back of the cam era. Either po rt can be the inlet or the out let. 3/8" I D Tygon tubing is suitabl e. Coolant Temperatu re: Coolant sho uld be no colder than the follow ing temperatures: PI -SCX :1300 +25°C. Operating a PI-S[...]

28 PI - SCX System Manual Version 2.E 28  S up er V G A m oni tor an d g rap hic s c ard s up po rt i ng a t lea s t 25 6 co lo rs w it h a t l eas t 1 Mby te o f m em o ry . Mem ory r eq u ir em en t i s d epe n d en t o n de sir e d d isp lay re sol uti o n .  Two-button Micro soft ® -com patible serial mouse or Log itech ® three-button s[...]

Chapter 4 System Setup 29 29 To Check the Con trolle r's Voltage Sett ing: 1. Look at the lowe r righthand corner on the rear o f the Control ler. The current v oltage setting (100, 120, 220, or 240 VAC) is displayed on th e Power Input Mo dule. 2. If the setting is co rrect, continu e with the ins tallation. I f it is not correct, fo llow the[...]

30 PI - SCX System Manual Version 2.E 30 PI -SCX:4300 On the bott om of the cam era towards t he rear are three threaded holes that can be used to m ount the cam er a. Two of the holes are M8 × 1.25 t ap (25 mm  deep) and the third one is 1/ 4  20 tap (0.5  deep ). Figure 9 show s the location of the mounting holes and their dis tan[...]

Chapter 4 System Setup 31 31 Setting up the Commun ication Interface PI - SCX cam era systems require e ither an installed P rinceton I nstruments (RSPI ) PCI card or an instal led USB2.0 inter face card in the hos t com puter. The type of interface card is dictated by the I nterface Control Modu le installed in the S T -133 con troller. Setting up[...]

32 PI - SCX System Manual Version 2.E 32 To Install the PC I Card Driver The following information assum es that you have a lready installed the WinView/3 2 or WinSpec/32 softw are. 1. After you hav e secured the PCI card in the com puter and replaced the cover, turn the computer on. 2. At bootup, Window s will try to install the new hardw are. If [...]

Chapter 4 System Setup 33 33 Microsoft driv er. Instructions for m aking the replacement are inc luded in "T o U p da te th e OrangeUSB USB 2.0 Driver" . To Update the OrangeUSB USB 2.0 Driver: This procedure i s highly recomm ende d when a laptop computer will be us ed to communicate w ith the ST-133. As s tated before, we recomm end the[...]

34 PI - SCX System Manual Version 2.E 34 To Install the Pr inceton Ins truments USB2 Interface: The following information assum es that:  You have v erified that the host computer meets the required spec ifications for USB 2.0 comm uni cation with the PI- SCX system (see pag e 28 ).  A USB 2.0 board and its driver are in stalled in the host c[...]

Chapter 4 System Setup 35 35 Connecting the Interface (Controller-Computer) Cable TAXI ® Cable (6050-0148-CE) Turn the Contro ller power OFF (OFF = 0, ON = | ) and the Compute r power OFF before connecting or di sconnecting the Cont roller-Com puter (TAXI) cable. To Connect the TA XI Cab le: 1. Verify that the C ontroller pow er is OFF. 2. Verify [...]

36 PI - SCX System Manual Version 2.E 36 Entering the Default Camera System Parameter s into WinX (W inV iew /32 , W i nS pec /32 , o r W inXT est / 32 ) Software changes implem ented in WinX version 2.15. 9.6 affected the w ay in which default param eters were ent ered for camera sy stems. Therefor e, two sets of in structions are included. Follow[...]

Chapter 4 System Setup 37 37 Figure 12. RSConf ig dialog 4. Open the Win X application and, from Setup|Hardwar e…, run the Hardware Setup wizard. 5. When the PVCAM dia log (Figure 13 ) is displayed, cl ick in the Yes radio b utton, click on Next and continue through t he wizard. Af ter the wizard is finished, the Controller/C amera tab ca rd will[...]

38 PI - SCX System Manual Version 2.E 38 To Secure the Tub ing: a. Remove the reta ining nuts f or the fitting and slide them ov er the outside of the plastic tubing. b. Slide the tubing over the barb on the fitting. c. Slide the retain ing nuts to t he end of the tubi ng and tighten them to the threads of the fitting. d. Insert the fitting into th[...]

Chapter 4 System Setup 39 39 Note: S ystems are now shipped with fittings that allo w you to select either a right angle or straig ht port co nfiguration. Figure 15. PI-SCX :4300 and PI-SCX:4096 C amera Back Pan el Do not disturb the Vacuum Pum pi ng fitting or the Indium- tipped Vacuum Seal on the PI - SCX:4300. Damag e to the vacuum fittings and [...]

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41 Chapter 5 Operation Introduction Once the PI- SCX camera has been in stalled, cam era operation is bas ically straightforward. I n most applicat ions you sim ply establish optim um perform ance using the Focus mode (WinVie w/32 or WinSpec /32), set t he target detecto r temperature, wa it until the tem perature has stabiliz ed at the set tem per[...]

42 PI - SCX System Manual Version 2.E 42  In A cquire mode, every fram e of data collected can b e automatical ly stored (the completed datase t may incl ude multiple fram es with one or m ore accumulations ). This mode would ordinarily be selected during actual data col lection. One limitati on of Acquire m ode operation is that if data acqu is[...]

Chapter 5 Operation 43 43 First Light The following paragraphs prov ide st ep -by- step instructions for verifying the opera tion of your PI-SC X system. The intent of th is simple proced ure is to he lp you gain basic familiarity w ith the operation of y our system and to show that it is function ing properly. The procedure do es not require an X-[...]

44 PI - SCX System Manual Version 2.E 44 Controller/Camera tab (Setup|Hard ware): Contr oller and Cam era parameters should be set autom at ically to the pr oper values fo r your system. H owever, you can click on the Load Defaults From Controller button on this tab to load the default settings.  Use PVCA M: If you are using the USB 2. 0 interfa[...]

Chapter 5 Operation 45 45 E x pe rim ent S e tu p T imi n g tab (A cq ui sit ion |Ex per im en t S e t up …) :  Timing Mode: F ree Run  Shutter Contro l: Normal  Safe Mode vs . Fast Mode : Fast Mode General tab (Displ ay|Layout…): Se lect Horizont al and Vertical Cross Sections. Acquiring Data If you are using WinView/32 and the comput[...]

46 PI - SCX System Manual Version 2.E 46 Power Down Procedure It is recomm ende d that the cam era warm-up be careful ly controlled. Prope r warm-up will be achieved by proc eeding as foll ows: 1. While running WinView, gradua lly bring the cam era tem perature up in 10°C steps from its curren t lock tem perature to ambient tem perature. To accom [...]

Chapter 5 Operation 47 47 Exposure Time Exposure tim e (set on the Experime nt Setup|Main tab) is the tim e between S tart Acquisition and S top Acquisition commands sen t by the application software to the camera. I n combination wi th triggers, t hese comm ands control when cont inuous cleaning of the CCD stops and when t he accumulated s ignal w[...]

48 PI - SCX System Manual Version 2.E 48 as st abl e c on tr o l is re- es ta bli sh ed . Thi s is n or m al b eh av io r an d sh ou ld n o t be a c a us e f o r co nc er n. Onc e te m pe rat ure lo ck i s e sta b l is h e d, th e te m p er at u r e is the rm os tat e d t o w ith i n ±0 .0 5° C. T h e c ont ro lle r i s eq uip pe d wi th an L ED [...]

Chapter 5 Operation 49 49 Saturation When signal lev els in som e part of the im age are very hig h, charge generat ed in one pixel may exceed the "well capacity" o f the pixel, sp illing over into adjacent pixe ls in a process called "bloom ing." I n this case a more frequent readou t is advisab le, with signal averaging to enh[...]

50 PI - SCX System Manual Version 2.E 50 The upper left drawing in Figure 19 represents a CCD after exposure but before the beg inning of readout. The capital letters repr esent diff erent amounts of charge, including both signal and dark charge. This secti on explains readou t at full reso lution, where every pixel is digitized separately. Readout[...]

Chapter 5 Operation 51 51 as image pixe ls, the binning of large sections m ay result in saturation and “bl oom ing”, or spilling of charg e back into the image area. Figure 20 shows an example of 2  2 bi nning. Each pix el of the im age displayed by the software represen ts 4 pixels of th e CCD array. Rectangular bins of any size a re possi[...]

52 PI - SCX System Manual Version 2.E 52 This technique i s also usefu l in high lig ht level experiments, wh ere the cam era is again photon shot- noise limited. Sum ming multiple pixels in software correspond s to collect ing more photons, and results in a be tter S/N ratio in the measu rement. Output Amplifier Selection The output am plifier amp[...]

Chapter 5 Operation 53 53 Example: The foll owing descr iptions assum e that the actual incoming lig ht level is identical in al l three instanc es. The numbers used il lustrate the effe ct of changing an analog gain settin g and do not ref lect actual perfor mance: gain at the Low, Mediu m, and High setting s depends on the CCD installed and the a[...]

54 PI - SCX System Manual Version 2.E 54 ADC Offset ADC offset (al so known as base line offset) provid es another way of dealing with d ark charge (see "Dark Charg e" , page 48). By offsetting the baseline signal, m uch of the background is ig nored during conversion. Offsetting the bas eline is ac complished by adding a voltag e to the [...]

55 Chapter 6 Advanced Topics Introduction Previous chapter s have discussed sett ing up the hardware and the so ftware f or basic operation. This chapter discus ses topics as sociated with experiment synchro nization (se t up on the Experime nt Setup |Timing tab in WinV iew and WinSpec).With the excepti on of Edge Trigg er, the topics are address e[...]

56 PI - SCX System Manual Version 2.E 56 Cleans m odes, opens the shutter a s soon as the S T-133 is ready to receiv e an Exter nal Sync pulse. This is required if the time between the External Sy nc pulse and the ev ent is less than a few m illiseconds, the t ime it takes th e shutter to open. The shutter tim ing is shown in the timing diagram s t[...]

Chapter 6 Advanced Topics 57 57 programm ed exposure period. As soon as the exposure is complete, the shu tter closes and the CCD array is read out. Because the ex ternal shutte r requires a finite amount of t ime to open com pletely (shutter open time m ay be 5-28 m s depending on the shutter ), the External Sync pulse trigger edge provided by th [...]

58 PI - SCX System Manual Version 2.E 58 Note: If EX T SYNC is still active ( in Figure 25, this m eans t hat if it is still LO W) at the end of the readou t, the hardw are may interpret th is as a second sy nc pulse, and so on. Figure 25. Timing D iagram for the External Sync Mode (- edge trigger) External Sync with Continuous Cleans Another tim i[...]

Chapter 6 Advanced Topics 59 59 completed, to prev ent the CCD from getting "ou t of step." As expec ted, the respon se latency is on the or der of one v ertical shift tim e, fro m 1- 30 µs depending on the array. This latency does not prevent th e incoming signal from being detected, sin ce photo- generated elec trons are still collecte[...]

60 PI - SCX System Manual Version 2.E 60 One disadvantage o f the Safe Mode is that events m ay be missed during the experi ment, since the ST - 133 is d isabled for a short time after e ach frame. Figure 28. Chart o f Safe Mode and Fa st Mode Operat ion[...]

Chapter 6 Advanced Topics 61 61 TTL Control Fully supported by WinView/WinS pec Version 2.5 wh en the commun ication protocol is TAXI (PCI), this fea ture is not suppor ted when the p rotocol is USB 2.0. Introduction Princeton I nstruments' Win View and WinSpec software packag es incorporates WinX32 Automation, a program m ing language that ca[...]

62 PI - SCX System Manual Version 2.E 62 Table 4 illustra tes this coding for decimal v alues 0 throug h 7. Obviously this t able could easily be extend ed to show the coding for values a ll the way to 255. Decimal Equiv. TTL IN/OUT 8 1= dec 128 TTL IN/OUT 7 1=dec 64 TTL IN/OUT 6 1=dec 32 TT L IN/OUT 5 1=dec 16 TTL IN/OUT 4 1=dec 8 TTL IN/OUT 3 1=d[...]

Chapter 6 Advanced Topics 63 63 Pin # Assignment Pin # Assignment 1 IN 1 14 IN 2 2 IN 3 15 IN 4 3 IN 5 16 IN 6 4 IN 7 17 IN 8 5 GND 18 GND 6 EN/CLK 19 Reserved 7 (future use) 20 GND 8 GND 21 OUT 2 9 OUT 1 22 OUT 4 10 OUT 3 23 OUT 6 11 OUT 5 24 OUT 8 12 OUT 7 25 GND 13 Reserved Figure 29. TTL In/Ou t Connector Table 5. TTL In/Out Connector Pin out T[...]

64 PI - SCX System Manual Version 2.E 64  Shielded Metaliz ed hood (Radio Sh ack part no 27 6-1536A).  BNC connec tor(s) type UG- 88 Male BNC connector (R adio Shack part no 27 8-103). Example Suppose you needed to build a cab le to monitor t he line TTL OU T 1. One approa ch would be to bui ld a cable as sembly as describ ed in the follow in[...]

65 Chapter 7 Troubleshooting Do not attach or r emov e any cables while th e PI-SCX system is powered on. Introduction The PI- SCX system is desig ned to be highly rel iable. Nevertheles s, problem s could develop. In m ost instances, the best recourse will be to contact the factory for g uidance. Most often the sy stem will be m ost reliably and q[...]

66 PI - SCX System Manual Version 2.E 66 No CCD Named in the Har dware Wizar d:CCD dialog (Versions 2.5.19 .0 and earlier) Page 79 Program Error message Page 79 Removing/Installing a P lug-In Module Page 80 Securing the Detector -Controller Cable Slide Latch Page 82 Serial violation s have occurred. Che ck interface cab le. Page 83 Baseline Signal [...]

Chapter 7 Troubleshooting 67 67 To change the de fault Camera Name : 1. Using Notepad or a similar tex t editor, open PVCAM. INI , which is lo cated in the Windows dire ctory (C:Windows, for ex ample). You shou ld see entrie s like the ones below. [Camera_1] Type=1 Name=Camera1 Driver=apausb.sy s Port=0 ID=523459 2. Change the "Nam e=" e[...]

68 PI - SCX System Manual Version 2.E 68 3. C on fir m th e fu se ra tin g s b y rem ov ing th e tw o w h it e f use h ol d e rs . T o do s o, s im p ly in ser t t h e f lat b la d e o f th e s cre w d riv er be hin d th e fr o n t tab of ea ch f u se ho ld er an d g en t ly p ry th e a s se m b ly ou t. Figure 33. Fuse Ho lder 4. Refer to the Fuse[...]

Chapter 7 Troubleshooting 69 69 Cooling Troubleshooting Temperature Lock Cannot be Achieved or Maintained. Operating a PI- SCX:1300 cam era with coolant at a t emperature colde r than specifi ed could cause induc ed condensation in the elect ronics enclosure and possible ca tastrophic damag e to the camera. Damage resulting from this ty pe of opera[...]

70 PI - SCX System Manual Version 2.E 70 deterioration in new cameras m ay be faster than in old ones. Fo r example, a c amera that has to be repum ped after perhaps a y ear of operation, m ay not have to be pumped again for several y ears. In any cas e you m ay notice a gradual dete rioration in tem perature control perform ance indicat ive of vac[...]

Chapter 7 Troubleshooting 71 71 Data Overrun Has Occurred message Because of mem ory constraints and the way that USB t ransfers data, a "Da ta overrun has occurred" m essage may be displayed during data ac quisition. If this m essage is dis played, take one or m ore of the follow ing actions: 1. Minimiz e the number of prog rams running [...]

72 PI - SCX System Manual Version 2.E 72 interface/cam era and is referenced by the Hardware Wizard when y ou are setting up WinView with USB for the first time: 1. I f y ou h av e n ot alr ead y d on e so , cl ose W in Vie w /3 2. 2. Ma ke su re t he S T- 1 33 i s conn ec te d to th e ho st com put e r an d th at it is tu rne d on . 3. Run RSConfi[...]

Chapter 7 Troubleshooting 73 73 Demo, High Speed PCI, and PCI(Timer) are Choices on Hardware Wizard:Interface dialog (Versions 2.5.19.0 and earlier) If there is an ins talled Princeton I nstruments (RSPI ) high speed card in the host co mputer and you want to op erate a cam era using the USB 2.0 i nterface, t he PVCAM.I NI file (created by RSC onfi[...]

74 PI - SCX System Manual Version 2.E 74 5. Using Notepad or a similar tex t editor, open P VCAM.INI , which is located in the Windows dire ctory (C:Windows, for ex ample). If the contents of the file look like: C ha ng e th e h ea din g s so th e c o n te n ts no w l o ok l ik e: [Camera_1] Type=1 Name=Cam era1 Driver=rspipc i.sys Port=0 [Camera_[...]

Chapter 7 Troubleshooting 75 75 Detector Temperature, Acquire, and Focus are Grayed Out (Versions 2.5.19.0 and earlier) These functions a nd others w ill be deactivated i f you hav e installed a cam era being run under USB 2.0 and h ave opened WinView/32 wit hout hav ing first turned on the ST - 133 . They will also be d eactivated if you hav e ins[...]

76 PI - SCX System Manual Version 2.E 76 Note: The [Cam era_#] must be chang ed so the cam era supported by the USB interface will be recognized (the US B driver is "apaus b.sys"). Fo r consistency, you may also want to chang e the camera nam es. 6. Save the file. Wi th the ST - 13 3 connected and on, open WinView/32. The formerly g rayed[...]

Chapter 7 Troubleshooting 77 77 interrupts have be en user assig ned. When a PCI card is installed, the BI OS checks for available address es and interrupt l evels and automatical ly assigns them so that there are no PCI address o r interrupt conf licts. However, bec ause the BIO S doesn't know about the user-assigned I SA I/O address and in t[...]

78 PI - SCX System Manual Version 2.E 78 Note: Changing the order of the PCI car ds, that is, plugging them into different slots, could change the ad dress and interrup t assignm ents and possibly resolv e the confl ict. However, this woul d be a trial and e rror process w ith no g uarantee of succe ss. Diagnostics Software Many diagnostic s progra[...]

Chapter 7 Troubleshooting 79 79 No CCD Named in the Hardware Wizard:CCD dialog (Versions 2.5.19.0 and earlier) Figure 43. Hardw are Wizard: De tector/Camera/CC D dialog If you hav e installed a USB 2.0 I nterface Modu le in your ST - 133 , a blank field may be displayed in th e Detector/Cam era/CCD dialog (Figure 43) if the S T-133 control ler was [...]

80 PI - SCX System Manual Version 2.E 80 6. Reboot your com puter. 7. Restart the W inX application and b egin acquiring data or focus ing. I f you see the message ag ain, increase the DMA buffer size. Removing/Installing a Plug-In Module The ST - 133 Controller has three p lug-in slots. The An alog/Contro l module (leftm ost slot when the contro l[...]

Chapter 7 Troubleshooting 81 81 To Remove a Module: 1. Ve r if y t ha t t h e C ont rol ler h as b ee n t urn ed O F F. 2. R ot ate th e t wo loc k i ng s cr ews (o ne a t the to p o f th e m od ul e a nd o ne a t t he b o tt om ) co un te rcl o c kw is e un ti l t h ey re lea s e f rom t he c ha ssi s. 3. Th e n , gr a sp th e m od u le a nd p ull[...]

82 PI - SCX System Manual Version 2.E 82 Securing the Detector-Controller Cable Slide Latch Som e Pri nce ton I nstrum ents D ete cto r- Co ntr oll er cab le s us e a s lide latc h to se cur e th e De tect or- Con tro ller ca b le to th e DE TEC TO R con nec tor on the ba ck o f the ST- 133. In co rrec tly plug g ing this cab le in to th e c on nec[...]

Chapter 7 Troubleshooting 83 83 Serial violations have occurred. C heck interface cable. Figure 46. Serial V iolations Have Occ urred dialog This error m essage dialog will appear if you t ry to acq uire an imag e or focus the cam era and either (or both ) of the follow ing conditions exists:  The camera syst em is not turned ON .  There is n[...]

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85 Appendix A Specifications CCD Arrays Types The following list is not necessar ily current. Other chips may also be available. Contac t the factory for up- to - date information. Model CCD (WinView /32 Name) Pixel Format Pixel Size CCD Type 1300 EEV CCD36- 40 (EEV 1300x134 0F) 1300 × 1340 20 × 20 µm 100 kHz/1 MHz, Full Frame 4300 Kodak KAF 430[...]

86 PI - SCX System Manual Version 2.E 86 PI -SCX: 4096 Array Size: 4096x409 6 Pixel Size: 15x15 µm Spectral Range ( typical): 400- 1000 nm Cooling: Water Output Amplifie r: Single port Parallel Shift Ra te: 200 µs /row Pixel Well: 70 K e - min, 85 Ke - typical Quantum Effici ency: PI -SCX: 4300 Array Size: 2084x208 4 Pixel Size: 24x24 µm Spectra[...]

Appendix A Specifications 87 87 Camera Cooling PI -S C X :1 300 : - 3 0° C (f o r ce d ai r ) to - 3 5° C (w a te r c oo le d a t + 25° C ) th erm o e le c tr ic (Pe lti e r) PI -SCX:4096: -50°C with +5°C water (≤1.5:1 fiber r atio); -45° C with +0°C water (>1.5:1 fiber rat io) PI -SCX:4300: -50°C with +5°C water Passive Cooling: CCD [...]

88 PI - SCX System Manual Version 2.E 88 Dimensions All dimensions are approxi mate and subject to change. Refe r also to the outline d rawings in Appendix B. PI -SCX:1300 4.63 in (11.8 cm ) widt h; 4.63 in (11.8 cm ) height; 8.71 in (22.15 cm ) length; 10 lb (4.54 kg) w eight PI -SCX:4300 6.00 in (15.24 cm ) widt h; 6.00 in (15.24 cm ) height; 8.4[...]

89 Appendix B Outline Drawings Note: All dimensi ons are in inches un less otherw ise noted. Figure 47. ST-133A Co ntroller Figure 48. ST-133B Co ntroller[...]

90 PI - SCX System Manual Version 2.E 90 Figure 49. PI-SCX :1300[...]

Appendix B Outline Drawings 91 91 Figure 50. PI-SCX :1300/PW[...]

92 PI - SCX System Manual Version 2.E 92 Figure 51. PI-SCX :1300/1.5PW[...]

Appendix B Outline Drawings 93 93 Figure 52. PI-SCX :4096 (100 mm Fiber Optic)[...]

94 PI - SCX System Manual Version 2.E 94 Figure 53. PI-SCX :4096 (165 mm Fiber Optic)[...]

Appendix B Outline Drawings 95 95 Figure 54. PI-SCX :4300 (75 mm Fiber Optic)[...]

96 PI - SCX System Manual Version 2.E 96 Figure 55. PI-SCX :4300 (165 mm Fiber Optic)[...]

97 Appendix C Repumping the Vacuum Introduction Fiberoptic taper CCD cameras are shipped with a v acuum level below 10 -5 Torr to assure proper cooling performance and to pr event condensat ion from col lecting on the C CD. In time, the vacuum level could deter iorate to where ach ieving temperature lock wi ll no longer be possib le. If this happen[...]

98 PI - SCX System Manual Version 2.E 98 Requirements  A laboratory- type vacuum pump capable of achi eving absolute pr essures below 10 mTorr. Your vacuum system m ust have a trap (ideally cry ogenic) placed b etween the cam era and the pump to prev ent contam ination due to oil ba ckstream ing from the pump.  5/16  hex torq ue wrench for[...]

Appendix C Repumping the Vacuum 99 99 Pumping Down 1. Do not bake during pumping . Maintain ambient tem perature (≈25° C). 2. Continue pumping for 48 hr. The final vacuum must be below 10 m T orr. At the factory, a much h igher initial vacuum l evel is genera ted using a turbo pump. Although this is des irable, it is not necessary and m ay be co[...]

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101 Appendix D USB 2.0 Limitations The following information cov ers the currently k nown limitations a ssociated with operating under the USB 2.0 interfac e.  Maximum cable length is 5 meters (16.4 fee t)  1 MHz is curr ently the upp er digitization ra te limit for the S T -133 Contro ller.  Large data sets and/or long acqui sition times [...]

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Declaration of Conformity We , ROPER SCIENT IFIC (PRINCETON INS TRUMENT S) 3660 QUAKERBR IDGE ROA D TRENTON, N J 08619 Declare under our s ole responsib ility, that the prod uct ST -133 CONTROL LER w/SCX CAMER A HEAD, To which this d eclaration relates , is in con formity with genera l safety require ment for elect rical equipment stand ards: IEC 1[...]

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105 Warranty & Service Limited Warranty Princeton I nstruments, a div ision of Roper Scien tific, Inc. ("Prince ton Instrum ents", "us", "we", "our") m akes the foll owing limited warr anties. These limited warran ties extend to the original purch aser ("You", "you ") only and no o the[...]

106 PI - SCX System Manual Version 2.E 106 Sealed Chamber Integrity Limited 12 Month Warranty Princeton I nstruments warrants the sealed cham ber integrity of a ll our product s for a period of twelv e (12) months afte r shipment. I f, at anytime within twelve (12) m onths from the date of d elivery, the de tector should exper ience a sealed cham b[...]

Warranty & Service 107 Owner's Manual and Troubleshooting You should read the owner’s m anua l thoroughly before operating this pro duct. I n the unlikely event that you should en counter difficu lty operating this product, the owner’s manual should be consulted befo re contacting the P rinceton I nstruments technica l support staff or[...]

108 PI - SCX System Manual Version 2.E 108 fo rg o in g w ar ra nty is ex c lu siv e an d in li e u o f a ll o t he r w arr a nt ies , g u a ra nte es, ag re em en ts , an d s im il a r ob li g a ti o n s o f m anu f ac tur er or sel le r w it h re s p ec t t o th e re pai r o r r ep la c em en t o f any p ar t s. I n no e v ent sh all P ri n ce t [...]

109 Index #-B 64 - pin D IN con nec to r ......................................... 17, 81 A/D converters ......................................................... 53 AC power requirements ........................................... 27 Air-circulation require ment ..................................... 16 Analog gain control .........................[...]

110 PI - SCX System Manual Version 2.E 110 Dark current ............................................................. 48 Declaration of Confor mity ..................................... 103 Detector connector (ST - 133) ................................... 19 DMA Buffer size ..................................................... 43 Dual A/D converter[...]

Index 111 Preopen Shutter mode .............................................. 57 Pr oc edu r es f am il iar i za ti on an d ch eck out .................................. 43 F ir st im ages ........................................................... 43 line voltage selection a nd line fuse ...................... 67 plug-in module installatio n/remov[...]

112 PI - SCX System Manual Version 2.E 112 USB 2.0 (cont.) data overrun ......................................................... 71 installation ............................................................ 34 in ter fa ce c ar d ......................................................... 21 V Vacuum deterioration ....................................[...]