Leica Sp9000 manuel d'utilisation

Instruction Manual Leica SP9000 V 2.2 English – 08/2007 Always keep this manual near the instrument. Read carefully prior to operating the instrument. Leica SP9000 Automatic knife sharpener[...]

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3 Leica SP9000 – Automatic knife sharpener The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and state- of-the-art technology as we understand it follow- ing thorough investigation in this field. We are un- der no obligation to update the present manual periodic[...]

4 Instruction manual V 2.2 – 08/2007 Table of contents 1. Important Not es .............................................................................................................. 5 1. 1 Symbols used in this manual and their meaning ............................................................... 5 1. 2 Designated use / mi suse .............[...]

5 Leica SP9000 – Automatic knife sharpener 1.1 Symbols used in this manual and Warnings appear in a grey box and are marked by a warning triangle: Useful notes, i.e. important user information appear in a grey box and are marked by an in- formation symbol: Figures in brackets refer to item num- bers in drawings or to the drawings themselves. Inst[...]

6 Instruction manual V 2.2 – 08/2007 General This instruction manual includes important instructions and information related to the operating safety and maintenance of the instrument. The instruction manual is an important part of the product. It must be read carefully before using the instrument for the first time and must always be kept with th[...]

7 Leica SP9000 – Automatic knife sharpener Potential hazard Caution: risk of injury when touch- ing the knives and blades as these are extremely sharp. Warning: Avoid touching live parts under any circumstances! 2 . 2 Safety instructions for hand- ling the instrument 2. Safety 2. 1 General information on instrument design and safe handling This i[...]

8 Instruction manual V 2.1 – 04/2003 3. Instruments properties 3.1 Technical Data Power supply : Overall Dimensions : Model 930 is 115 V / 60 Hz Width : 370 mm Model 940 is 230 V / 50 Hz Height : 460 mm Depth : 540 mm 3.2 General Description Your technical skill is the key to the successful performance of any labora- tory equipment. While the Lei[...]

9 Leica SP 9000 – Automatic knife sharpener 3. Instrument properties 3.3 Overview - SP9000 Plastic cover Glass Plate Glass plate thumbscreen Plate positional dial Knife holder Hone glass plate holder Timer Off Lap Fine Coarse[...]

10 Instruction manual V 2.2 – 08/2007 4. Installation 4.1 Check list The Leica Microtome Knife Sharpener is shipped with all accessories re- quired for coarse and fine knife honing of knife edges and for dressing glass hone plate surfaces when necessary. 1 . Coarse abrasive, 2 bottles, for coarse honing procedure. 2. Fine abrasive, 2 bottles, for[...]

11 Leica SP 9000 – Automatic knife sharpener 4.2 Installing the instrument 1. Place the sharpener on a sturdy table or laboratory bench and remove tags and retaining tape used for shipment. 2. Assure that the plastic cover fits securely upon the base and is firmly supported when opened. 3. Be sure that the push-button switch is „OFF”. Plug th[...]

12 Instruction manual V 2.2 – 08/2007 Key to sharpening success The starting point of any successful sharpening technique is the careful ex- amination of the condition of the knife edge using a microscope. Periodic re- examination during sharpening is equally important. Please remember ! The width of the entire cutting facet is only from 0.1 to 0[...]

13 Leica SP 9000 – Automatic knife sharpener For the first resharpening of a new Leica knife or one that has been factory reconditioned by Leica, the honing process must begin with coarse honing. This is necessary in order to develop the sec- ond facet as is explained in the section on ,,Factory Recondition- ing”. Again, please note that micros[...]

14 Instruction manual V 2.1 – 04/2003 5.2. Put honing plate on holder Two plates are supplied, each with two usable sides. When the plate is properly positioned, tighten the thumbscrew. (Figure 14) 5.3. Apply coarse abrasive to the plate (Shake the abrasive very thoroughly until all particles are in suspension. If necessary remove dispenser top a[...]

15 Leica SP9000 – Automatic knife sharpener Fig. 15.1 Fig. 15.2 5.4. Attach knife With the two clamps facing ,up” and clamp screws loosened, install knife so that the end with the Leica trademark to your right ( Figure 15.1 ). This places the slotted end of the knife to your left as you face the front of the Leica Knife Sharpener. Tighten the t[...]

16 Instruction manual V 2.2 – 08/2007 5.6 Set coarse honing knob Switch the coarse honing knob on the left-hand side of the control panel fully to the left so the direction of the knob is towards „Coarse”. 5.7 Set Timer at 30 minutes When beginning coarse honing operation, set the automatic timer for 30 mi- nutes. This initial setting of 30 m[...]

17 Leica SP 9000 – Automatic knife sharpener 5.10 Clean knife - inspect condition Wipe knife with clean cloth moistened with solvent and inspect the cutting facet under a microscope (1OOX). Keeping in mind that the coarse honing action proceeds from the back of the facet to the edge of the knife, check to see how much of the surface has been hone[...]

18 Instruction manual V 2.1 – 04/2003 6. Fine coarse procedure Coarse honing must be followed by fine honing using the Leica Fine Abra- sive. As illustrated in Figure 10, the coarse honing action produces a single facet. In proceeding with the fine honing operation, a second cutting facet is ground on the knife at the cutting edge as shown in Fig[...]

19 Leica SP 9000 – Automatic knife sharpener The fine honing procedure is identical to coarse honing with three excep- tions: 1. Lower honing plate to fine honing position by turning honing dial on the left-hand side of the control panel fully to the right. Note red dot. 2. Apply a ribbon of the Leica Fine Abrasive to the plate. After extensive t[...]

20 Instruction manual V 2.2 – 08/2007 6. Fine coarse procedure After Fine Honing is Complete Shut off instrument by depressing ,OFF” button. Clean knife and wipe dry care- fully (Figure 20.1) . Where atmosphere is corrosive and the knife is to be stored for any length of time, lubricate with a good grade of light, neutral oil. After the knife h[...]

21 Leica SP 9000 – Automatic knife sharpener Abb. 21.1 Abb. 21.2 7. Redressing hone glass plates In order to eliminate excessive redressing time on the hone plates, it is sug- gested that users accumulate no more than three or four hours sharpening time on any plate surface. The honing action of the knife against the frosted surface of the glass [...]

22 Instruction manual V 2.2 – 08/2007 3. If the plastic glass retainers are not in place, install them on the posts on the platform and tighten the two thumb screws in each one (Figure 15). The two glass retainers may be left in place during knife sharpen- ing. 4. Position one hone glass on the plate holder and tighten thumbscrew (fin- ger tight [...]

23 Leica SP 9000 – Automatic knife sharpener 7. Insert the two lips of the bridge assembly into the slot behind the front panel. Then, with the spring mounted suction cups on the top plate, press down on the bridge and tighten the thumb screw (Figure 19). 8. Set the timer for 10 minutes and push the „LAP“ button. 9. When the machine stops, wa[...]

24 Instruction manual V 2.2 – 08/2007 The new, or factory reconditioned, Leica Knife has very precise wedge and cutting facet angles. These angles permit the knife to ,meet“ the glass hone plate in the correct manner for proper coarse and fine honing. For this reason, please remember : 1. The use of only new or factory reconditioned Leica Knive[...]

25 Leica SP 9000 – Automatic knife sharpener Should the occasion ever arise, the Leica Knife Sharpener can be re- conditioned at the Leica factory in Nussloch, Germany or at many of the Leica Microsystems Technical Service Centres worldwide. This is a cus- tomer service provided at a nominal charge. 1 . CLEANING : Plastic cover and outside enamel[...]

26 Instruction manual V 2.2 – 08/2007 Appearance Problem Cause Correction 8. Trouble shooting Fine facet fades out at ends of knife (opposite side of knife has even bevel). or Fine facet fades out at centre of knife (opposite side of knife has even bevel). Microscopic curvature on one side of knife. Continue coarse honing. 1. Recondition at facto[...]

27 Leica SP9000 – Automatic knife sharpener 9. Warranty and service Warranty Leica Biosystems Nussloch GmbH guarantees that the contractual prod- uct delivered has been subjected to a comprehensive quality control proce- dure based on the Leica in-house testing standards, and that the product is faultless and complies with all technical specifica[...]

28 Instruction manual V 2.2 – 08/2007 . . . . . . . . . . . . . . . . . . . . . . . . . . . . Anne De Greef-Safft President Biosystems Division Leica Biosystems Nussloch GmbH Postfach 1120 D-69222 Nussloch October 11, 2007 We herewith declare, in exclusive responsibility, that the instrument Leica SP9000 – Automatic Knifesharper was developed, [...]

29 Leica SP 9000 – Automatic knife sharpener Peoples Republic of China[...]

30 Instruction manual V 2.2 – 08/2007 Notice[...]