Leica 106Z manuale d’uso

Leica Stereo- Fluorescence Systems User Manual[...]

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3 Contents Page Leica MZ10 F fluorescence stereomicroscope Description .............................................................. 4 Safety concept .......................................................... 4 Fluorescence applications ................................................ 6 Components, controls ........................................[...]

4 Description Fluorescence technique Some substances fluoresce when irradiated with short-wave light. This property is utilized in the fluorescence technique, in which certain structures and features which do not fluo- rescence can be tagged with a fluorescing dye. An example is green-fluorescing protein (GFP), which is used in molecular biology . [...]

5 Servicing and repairs – Repairs may only be carried out by Leica-trained service technicians or by technical personnel authorized to do so by the person in charge of the instrument – Only original Leica spare parts may be used – Unplug the power cable before opening the supply unit. T ouching live circuits can cause injury . Legal requireme[...]

6 Applications Natural science Application Fluorescence filter set Anatomy •Monitoring of capillaryflow •GFP 1, GFP2 Biology •Gene expression inchicken embryos, fruitflies,  •GFP 1, GFP2 threadworms and zebra fish  •Fish otoliths markedwith alizarine[...]

7 Industry Application Fluorescence filter set Electronics •Solder paste onSMDs •GFP 1, GFP2  •Epoxy resin onSMD plates •GFP 1, GFP2  •Luminescent coating ofTV monitor tubes •GFP 1, GFP2  •Quality of polymercastings for[...]

8 Overview Leica MZ10 F: Its components and controls 1. Choice of stand and of focusing drive (manual or motor -driven) 2. Microscope carrier for Leica MZ10 F 3a. Leica MZ10 F optics carrier with third beam path 3b. Integrated FLUOIII filter system 3c. Adapter for light source 3d. Rapid filter changer for any of four sets of filters 3e. Slots for l[...]

9 Filter sets Designation Excitation filter Barrier filter GFP fluorescence GFP1 425/60 nm 480 nm GFP Plus fluorescence GFP2 480/40 nm 510 nm GFP plant fluorescence GFP3 470/40 nm 525/50 UV fluorescence UV 360/40 nm 420 nm Violet fluorescence V 425/40 nm 475 nm Blue fluorescence B 470/40 nm 515 nm Green fluorescence G 546/10 nm 590 nm Filter carrie[...]

10 Leica MZ10 F Assembly 1. Incident- or transmitted-light base 2a. Manual focusing drive, or 2b. Motorized focusing drive 3. Microscope carrier for Leica MZ10 F 4. Leica MZ10 F optics carrier with FLUOIII™ filter system 5a. Lamp housing 106Z 5b. Stray-light protection 6. Binocular tube 7. UV protection screen with arm 8. 4 sets of filters 9. Lig[...]

11 Leica MZ10 F Focusing drive ➜ base of stand ▶ Connect manually-operated or motor -driven focusing drive, complete with column, to base, as described in user manual M2-105-0EN. Before using the motor focus, you must read the safety directions in the accompanying user manual M2-267- 104. Microscope carrier ➜ focusing drive ▶ Fit microscope[...]

12 Assembly Lamp housing The Leica 106Z lamp housing is equipped. For information about the supply units for the 106Z lamp housing, see page 14. Model 105Z lamp housings, if available, can also be used. Observe the safety regulations on page 5 Notes and recommendations • Donot switch onand off morethan necessary,beca[...]

13 ▶ Using the focusing knob, displace the collector: • Duringthis movement, the collector must not touch the flexible power cable. If necessary , bend the cable away ▶ Carefully close the lamp housing, making sure that the dis connect plug engages the socket ▶ Press in the disconnect plug slightly ▶ T ighten the screws ▶[...]

14 Assembly Supply unit for lamp housing 106Z with 50W mercury-vapour burner 1. On/off switch 2. Running-time meter 3. Selector for L1/L2 burners 4. Fuse 5. Selectorfor 50Hz/60Hz 6. Connector for lamp 7. Power cable The supply unit is intended for power supplies above 220V . T o run it on lower voltages a step-down transformer is required [...]

15 3 1 2 4 5 6 Hg50W Hg 100W Adjusting the mercury vapour lamp This adjustment is important for obtaining a uniform light spot and good-quality fluorescence. While adjusting the mirror image, do not project light on to the electrodes for long periods (risk of explosion through overheating) The two electrodes are imaged as an extension of the [...]

Dummy filter carrier In the dummy filter carrier there are two openings for the observation beam paths instead of the barrier filters. The third path has been closed. Use the dummy filter carrier: – if you are using less than four sets of filters – if you are working without the fluorescence illuminator for periods of up to 15 seconds. • D[...]

17 Use MZ10 F: Bringing the specimen into focus and observing it Observe the safety directions on page 4 and the recom- mendations on page 5 regarding the use of the mercu- ry-vapour lamp. Changing filters In the rapid filter changer , all four positions must be occupied, either with filter sets or with dummy filter carriers (see page 16). The acti[...]

18 Overview Outfit with stereo-fluorescence module The components 1 Choice of stand and of focusing drive (manual or motor -driven) 2 Microscope carrier 3 Leica MS5, MZ6, MZ7.5 – 16 optics carrier 4a Leica stereo-fluorescence module with choice of barrier fil- ter 4b Filter insert for excitation filter 4c Light stop 4d Filter holder with an indiv[...]

19 Filter sets Excitation Dichromatic Barrier filters beam splitters filters GFP fluorescence 425/60nm 470nm GG475 GFP-Plus fluorescence 480/40nm 505nm LP 510nm LP GFP-Plant 470/40nm 495nm 525/50nm UV fluorescence 360/40nm 400nm GG420 Violet fluorescence 425/40nm 460nm GG475 Blue fluorescence 470/40nm 505nm OG515 Green fluorescence 546/10nm 565nm O[...]

20 Assembly Stereo-fluorescence module 1 Incident- or transmitted-light base 2a Manual focusing drive, or 2b Motorized focusing drive 3 Microscope carrier 4 Choice of optics carrier 5 Fluorescence module 6a 106Z lamp housing 6b Stray-light protection 7 Binocular tube 8 Clamp 9 Arm 10 UV-light excluder 11 Filter carrier 12 Filterholder for excitatio[...]

21 Important: With the illuminator on, insert the filterhol- der with diaphragm into slide-in unit 3 to avoid being suddenly exposed to excessively bright light (page 22). ▶ Remove the binocular tube. ▶ Loosen the 3 socket-head screws. ▶ Remove the cover . ▶ Loosen the fastening screw and take out the filter carrier . ▶ Insert the desired[...]

22 Assembly Stereo-fluorescence module UV-light excluder ➜ side-faced column Always position the UV-light excluder so that the user never looks directly at the light spot (page 5). ▶ Using the clamping screw , secure the clamp on the side- faced column, beneath the focusing drive. ▶ Slacken the central fixing screw . ▶ Unfold the arm. ▶ T[...]

23 Use Stereo-fluorescence module Focusing and observing Observe the safety regulations on page 4 and note the recommendations on page 5 for using the mercury- vapour burner . Position the UV-light excluder correctly (see page 22): ▶  Holdthe arm ▶ Release the central clamping screw ▶ Position the UV-light excluder in front of the ob[...]

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